Fluoroskan and Luminoskan Microplate Readers | Thermo Fisher Scientific

Fluoroskan, Fluoroskan FL and Luminoskan instruments are all equipped with powerful Thermo Scientific SkanIt Software, offering increased usability for setting up protocols and data analysis. Use verified protocols from the growing SkanIt Cloud Library to help save time and make your workflow more efficient. The Thermo Scientific Fluoroskan Microplate Fluorometer is a compact, robust fluorescence plate reader built to run variety of fluorometric and FRET assays with excellent performance. The Thermo Scientific Luminoskan Microplate Luminometer is a sensitive, DLReady certified luminescence plate reader built to perform superbly for a variety of luminescence applications, including flash, glow, and BRET assays. The DLReady-certified Thermo Scientific Fluoroskan FL Microplate Fluorometer and Luminometer is a dual-mode versatile workhorse for fluorometric and luminometric assays.

Features

In addition to the common features of all Microplate Reader instruments, the Fluoroskan FL, Fluoroskan fluorometric, Luminoskan Microplate Readers offer:

Reagent dispensers: simultaneous dispense and read

Enables easy and accurate reagent addition simultaneously as the instrument reads—a critical feature for flash-type reactions. All Fluoroskan and Luminoskan models are offered with three options of associated reagent dispensers: two dispensers, one dispenser, or no dispensers.

Flexibility to read 6- to 384-well plates

Allows you to choose your sample format depending on your unique research and throughput needs.

Auto-calibration and self-diagnostics

Enables accurate fluorescence data with every run. If something isn't quite right, all three reliable fluorescence and luminescence plate readers can tell you before your run.

High sensitivity for both top and bottom reading

Fiberless direct illumination optics for both top and bottom reading. Top reading is great for homogeneous, BRET and FRET assays, whereas bottom-reading becomes very important for non-homogeneous and cell-based assays. Bottom reading shortens the distance between the cell layer at the bottom of the well and the detector and minimizes any effect from the cell culture medium.

Dynamic range >6 decades

Reliably measure high and low signal samples with Fluoroskan and Luminoskan readers that provide both sensitivity and broad dynamic range in the same run.

Incubation and shaking

Incubate up to 45°C with orbital shaking.

Sample image

Multipoint well reading

Read multiple locations in a single well by stipulating in SkanIt Software where in the well you want to read. Multipoint measurements on Thermo Scientific fluorescence plate readers can also be used as an alternative or quantitative complement to imaging fluorospot assays.

Specifications

Technology	Specification	Fluoroskan	Luminoskan	Fluoroskan FL

Fluorometry	Excitation wavelength range	320–700 nm		320–700 nm
	Emission wavelength range	360–800 nm		360–670 nm
	Excitation filters	Up to 8 filters in the excitation filter wheel; 355 nm and 485 nm filters included as standard. Other filters available upon request.		Up to 8 filters in the excitation filter wheel; 355 nm and 485 nm filters included as standard. Other filters available upon request.
	Emission filters	Up to 8 filters in the emission filter wheel; 460 nm and 538 nm filters included as standard. Other filters available upon request		Up to 6 filters in the emission filter wheel. 460 nm and 538 nm filters included as standard. Other filters available upon request.
	Sensitivity	2 fmol fluorescein/well in a black 96-well plate		2 fmol fluorescein/well in a black 96-well plate
	Dynamic range	>6 decades		>6 decades
Luminometry	Spectral range		270–670 nm	270–670 nm
	Sensitivity		10 amol ATP/well using flash reaction, white 384-well plate	40 amol ATP/well using flash reaction, white 384-well plate
	Dynamic range		>6 decades	>6 decades
	Luminometric filters		Up to 6 luminometric filters in the filter wheel. All filters available upon request.
General features	Number of dispensers	Up to 3*	Up to 3*	Up to 3*
	Dispensing volume	4–995 μL in 1 μL increments	4–995 μL in 1 μL increments	4–995 μL in 1 μL increments
	Dispensing speed	30 s, 96-well plate, 5 μl/well	30 s, 96-well plate, 5 μl/well	30 s, 96-well plate, 5 μl/well
	Plate types	6- to 384-well plates	6- to 384-well plates	6- to 384-well plates
	Measurement speed	18 s, 96-well plate 60 s, 384-well plate	18 s, 96-well plate 60 s, 384-well plate	18 s, 96-well plate 60 s, 384-well plate
	Wavelength selection	Filters	Filters	Filters
	Light source	Quartz-halogen lamp		Quartz-halogen lamp
	Detector	Photomultiplier tube	Photomultiplier tube	Photomultiplier tube
	Incubator	From ambient + 3°C to 45°C, at ambient 25°C	From ambient + 3°C to 45°C, at ambient 25°C	From ambient + 3°C to 45°C, at ambient 25°C
	Plate shaking	Orbital	Orbital	Orbital
	User interface	PC software (SkanIt software)	PC software (SkanIt software)	PC software (SkanIt software)
	Computer interface	USB 2.0	USB 2.0	USB 2.0
	Dimensions (H x W x D)	340 x 420 x 420 mm 13.4 x 16.5 x 16.5 in.	340 x 420 x 420 mm 13.4 x 16.5 x 16.5 in.	340 x 420 x 420 mm 13.4 x 16.5 x 16.5 in.
	Weight	Basic unit 21 kg (46 lb) 2 optional dispensers add 2.4 kg	Basic unit 20.5 kg (45 lb) 2 optional dispensers add 2.4 kg	Basic unit 21 kg (46 lb) 2 optional dispensers add 2.4 kg

Ordering information

For Research Use Only. Not for use in diagnostic procedures.