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Currently available in North America, Europe, Middle East, Africa, and Greater China. Other regions coming soon.
Our newest multimode microplate reader is a versatile, entry level multimode microplate reader for most common absorbance, turbidimetric, fluorescence, and luminescence microplate assays. Photometric optical design is based on a xenon flash lamp and monochromator for enabling continuous wavelength selection from the UV through the visible range up to 1,000 nm. The broad wavelength ranges enable many common assays including nucleic acid and protein quantification, bacterial growth curves, ELISA, and cell viability
In Varioskan ALF, fluorescence and luminescence measurements are performed with filter-based optics for optimal specificity and sensitivity. The system comes with three pre-installed excitation and emission filter sets suitable for many common fluorometric applications like Thermo Scientific Quant-IT DNA quantitation assays, PrestoBlue and AlamarBlue cell viability assays. Additionally, five open filter positions are available for customized fluorometric and luminometric measurements. Filter slots are easy to access from the instrument side and no additional tools are required for filter exchange. Also commonly used FRET and BRET assays are easy to set up and perform thanks to Varioskan ALF unique filter-based optics design.
The automatic gain adjustment feature selects the ideal fluorometric or luminometric reading range for your instrument based on signal intensity in the well, eliminating the need to manually adjust measurement parameters. The results are consistent no matter what signals are measured.
Features
In addition to being fast, flexible, and easy to use, the Varioskan ALF multimode reader also features:
- Kinetic and endpoint measurements for a variety of applications.
- Spectral scanning for photometric measurements.
- Separate optimized measurement modes for absorbance and turbidimetric measurements.
- A self-diagnostics system includes set of initialization tests and adjustments ensuring instrument readiness for operation.
- Compatible with 6 to 384-well microplates with and without lids.
- Reads µDrop and µDrop Duo plates for microvolume analysis of DNA, RNA, and proteins.
- Performs onboard shaking and incubation for temperature-critical assays.
- Operated through SkanIt software
- Unlimited installation on multiple computers, no annual fee required.
- Includes an extensive cloud-based library of ready-made protocols.
- Two editions available—Research Edition for life sciences, Drug Discovery Edition with features for compliance with 21 CFR Part 11.
- Multiple user interface languages available.
- Automation compatibility for integration with automation interface.
- Thermo Scientific verification plates provide comprehensive and traceable validation of the instrument optical performance. Ready-made SkanIt software protocols provide easy to read reports with defined measurement parameter limits.
Specifications
| Category | Attribute | Description |
| Absorbance | Plate types | 6-384 well plates, Thermo Scientific µDrop Plates |
| Wavelength selection | Monochromator | |
| Wavelength range | 200–1,000 nm | |
| Light source | Xenon flash lamp | |
| Read out range | 0–4 Abs | |
| Linearity at 450 nm | 0–2.5 Abs, 2% | |
| Accuracy at 450 nm | 1.0% + 0.003 Abs (0–2.0 Abs), 2.0% (2.0–2.5 Abs) |
|
| Precision at 450 nm | SD <0.003 Abs or CV <1.0% |
|
| Measurement speed (starting from A1 back to A1) | 96-well microplates: ≤10 seconds @ fast mode | |
| Spectral scanning speed (Absorbance) | ≤16 s from 200 to 1000 nm with 1 nm steps @ fast mode | |
| Fluorescence Intensity | Plate types | 6–384-well plates |
| Wavelength selection | Filters | |
| Excitation wavelength range | 200–750 nm | |
| Emission wavelength range | 210–760 nm | |
| Light source | Xenon flash lamp | |
| Dynamic range | >6 decades | |
| Measurement speed (starting from A1 back to A1) | 96-well microplates: ≤20 seconds | |
| Luminescence | Plate types | 6–384 well plates |
| Wavelength selection | Direct or filters | |
| Wavelength range | 210–760 nm | |
| Dynamic range | >6 decades | |
| Measurement speed (starting from A1 back to A1) | 96-well microplates: ≤20 seconds | |
| Incubator and Shaker | Temperature range | From ambient + 4 °C to 45 °C |
| Shaking type | Linear, orbital, double orbital | |
| General Features | Measurement modes | End-point, kinetic, spectra, multipoint and kinetic spectra |
| Interface | PC software (SkanIt software) | |
| Dimensions (D x W x H) | 343 mm (W) × 610 mm (D) × 402 mm (H) | |
| Weight | 25 kg |
For Research Use Only. Not for use in diagnostic procedures.