Ready-to-use standards enable quick instrument calibration

by John C. Rogers, Ph.D.; Michael M. Rosenblatt, Ph.D.; Eugene J. Cichon, B.S.; Rosa Viner, Ph.D.*; Monica Noonan, M.S. - 06/01/11

Routine calibration of mass spectrometers is required for optimal performance. Our new calibration solutions for Thermo Scientific Mass Spectrometers are ready-to-use liquid formulations for quick and easy instrument calibration.

Calibration solutions for Thermo Scientific Ion Trap and Orbitrap Mass Spectrometers

Calibration of Thermo Scientific Ion Trap and Orbitrap Mass Spectrometers is required for proper instrument performance. These high-quality calibration solutions are for regulated environments and busy laboratories and avoid time-consuming reagent preparation. Reagents are provided in high-purity Teflon*-coated containers tested rigorously to provide the highest batch-to-batch reproducibility (Figures 1 and 2).

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Figure 1. Representative mass spectrum for the Thermo Scientific Pierce LTQ Velos ESI Positive Ion Calibration Solution. N-butylamine, caffeine, Met-Arg-Phe-Ala (MRFA) and Ultramark 1621 are used to calibrate Thermo Scientific LTQ Velos Ion Trap and Orbitrap Mass Spectrometers in the ESI positive ionization mode. The N-butylamine is included to extend to low mass calibration range.

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Figure 2. Representative mass spectrum for the negative ion calibration. Sodium dodecyl sulfate, sodium taurocholate and Ultramark 1621, comprise the calibration mixture used to calibrate Ion Trap and Orbitrap* Mass Spectrometers in the negative ionization mode.

Calibration solutions for Thermo Scientific Triple Quadrupole Mass Spectrometers

Thermo Scientific TSQ Mass Spectrometers are calibrated with multimers of tyrosine (Tyr1, Tyr3, Tyr6). All of the components of this mixture are prepared from the highest purity raw materials and are stored in inert Teflon-coated containers (Figure 3).

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Figure 3. Representative mass spectrum for the triple-quadrupole MS calibration. Multimers of tyrosine are used to calibrate the TSQ series of Triple Quadrupole Mass Spectrometers.

LTQ ESI Positive Ion Calibration Solution

The Thermo Scientific Pierce LTQ ESI Positive Ion Calibration Solution is a mixture of highly purified ionizable molecules specifically designed for positive mode calibration of Thermo Scientific ion trap and orbitrap instruments.

Features of LTQ ESI Positive Ion Calibration Solution:

  • Strong peaks—mixture of caffeine, MRFA and Ultramark 1621 in a acetonitrile/methanol/acetic solution.
  • Ready-to-use—load the mass reference standard into a syringe and inject into the instrument
  • High-purity—mass spectrometry grade reagents in a non-leachable container
  • Stable—store at room temperature for more than one year

Learn more about Thermo Scientific Pierce LTQ ESI Positive Ion Calibration Solution

For Research Use Only. Not for use in diagnostic procedures.