The TargetSeq™ Exome Enrichment System Provides Efficient Targeted Sequencing

For Research Use Only. Not intended for any animal or human therapeutic or diagnostic use.

Step-by-Step Guide to Targeted Resequencing Products

Decide on important experimental considerations: what type of variation you want to detect, in how many genes (or target regions), and how many samples?

Learn More:

Enrich your DNA using one of the following methods: PCR, long-range PCR, array hybridization, in-solution hybridization, or chromosome sorting. After enrichment, consider multiplexing strategies if you have multiple samples to process.

SequalPrep™ Long PCR kit and Normalization plate

Complete long PCR enrichment and amplicon normalization system that provides a highly efficient enrichment solution to meet NGS re-sequencing throughput capabilities.

Learn More:

Learn More:

User documentation for the 5500 Series Genetic Analysis Systems can be found on the SOLiD Community. Access is through the 5500 Series User Hub. Access is limited to 5500 Series Genetic Analysis Systems users who are registered on the solid community and have been authenticated through the process of requesting permission to join the 5500 Series User Hub.

The tools you need for each step in the targeted sequencing data analysis workflow:

 
Data Analysis StepApplied Biosystems Software3rd-Party Software***
1. Align reads to reference in color space
  • ICS
  • LifeScope™ Genomic Analysis Software
  • LifeScope™ Cluster
  • LifeScope™ Workstation
 
2. Generate quality metrics and perform mate pair rescue
  • LifeScope™ Genomic Analysis Software
  • LifeScope™ Cluster
  • LifeScope™ Workstation
 
3. Generate sequencing and alignment statistics
  • LifeScope™ Genomic Analysis Software
  • LifeScope™ Cluster
  • LifeScope™ Workstation
 
4. Identify Polymorphisms
  • LifeScope™ Genomic Analysis Software
  • LifeScope™ Cluster
  • LifeScope™ Workstation
 
5. Translate color space to base space 
6. Visualize in context of annotation
  • LifeScope™ Genomic Analysis Software
  • LifeScope™ Cluster
  • LifeScope™ Workstation
7. Convert to SRF for publishing  
*** Need XSQ --> csfasta/qual converter

Learn more

Validate results using our BigDye® Direct Cycle Sequencing Kit, for Sanger sequencing on our capillary electrophoresis-based DNA sequencers.

BigDye® Direct Cycle Sequencing Kit

Decide on important experimental considerations: what type of variation you want to detect, in how many genes (or target regions), and how many samples?

Learn More:

Enrich your DNA using one of the following methods: PCR, long-range PCR, array hybridization, in-solution hybridization, or chromosome sorting. After enrichment, consider multiplexing strategies if you have multiple samples to process.

SequalPrep™ Long PCR kit and Normalization plate

Complete long PCR enrichment and amplicon normalization system that provides a highly efficient enrichment solution to meet NGS re-sequencing throughput capabilities.

Learn More:

Learn More:

User documentation for the 5500 Series Genetic Analysis Systems can be found on the SOLiD Community. Access is through the 5500 Series User Hub. Access is limited to 5500 Series Genetic Analysis Systems users who are registered on the solid community and have been authenticated through the process of requesting permission to join the 5500 Series User Hub.

The tools you need for each step in the targeted sequencing data analysis workflow:

 
Data Analysis StepApplied Biosystems Software3rd-Party Software***
1. Align reads to reference in color space
  • ICS
  • LifeScope™ Genomic Analysis Software
  • LifeScope™ Cluster
  • LifeScope™ Workstation
 
2. Generate quality metrics and perform mate pair rescue
  • LifeScope™ Genomic Analysis Software
  • LifeScope™ Cluster
  • LifeScope™ Workstation
 
3. Generate sequencing and alignment statistics
  • LifeScope™ Genomic Analysis Software
  • LifeScope™ Cluster
  • LifeScope™ Workstation
 
4. Identify Polymorphisms
  • LifeScope™ Genomic Analysis Software
  • LifeScope™ Cluster
  • LifeScope™ Workstation
 
5. Translate color space to base space 
6. Visualize in context of annotation
  • LifeScope™ Genomic Analysis Software
  • LifeScope™ Cluster
  • LifeScope™ Workstation
7. Convert to SRF for publishing  
*** Need XSQ --> csfasta/qual converter

Learn more

Validate results using our BigDye® Direct Cycle Sequencing Kit, for Sanger sequencing on our capillary electrophoresis-based DNA sequencers.

BigDye® Direct Cycle Sequencing Kit

Application Notes

Posters

Publications

Step-by-Step Guide to Targeted Resequencing Products

Decide on important experimental considerations: what type of variation you want to detect, in how many genes (or target regions), and how many samples?

Learn More:

Enrich your DNA using one of the following methods: PCR, long-range PCR, array hybridization, in-solution hybridization, or chromosome sorting. After enrichment, consider multiplexing strategies if you have multiple samples to process.

SequalPrep™ Long PCR kit and Normalization plate

Complete long PCR enrichment and amplicon normalization system that provides a highly efficient enrichment solution to meet NGS re-sequencing throughput capabilities.

Learn More:

Learn More:

User documentation for the 5500 Series Genetic Analysis Systems can be found on the SOLiD Community. Access is through the 5500 Series User Hub. Access is limited to 5500 Series Genetic Analysis Systems users who are registered on the solid community and have been authenticated through the process of requesting permission to join the 5500 Series User Hub.

The tools you need for each step in the targeted sequencing data analysis workflow:

 
Data Analysis StepApplied Biosystems Software3rd-Party Software***
1. Align reads to reference in color space
  • ICS
  • LifeScope™ Genomic Analysis Software
  • LifeScope™ Cluster
  • LifeScope™ Workstation
 
2. Generate quality metrics and perform mate pair rescue
  • LifeScope™ Genomic Analysis Software
  • LifeScope™ Cluster
  • LifeScope™ Workstation
 
3. Generate sequencing and alignment statistics
  • LifeScope™ Genomic Analysis Software
  • LifeScope™ Cluster
  • LifeScope™ Workstation
 
4. Identify Polymorphisms
  • LifeScope™ Genomic Analysis Software
  • LifeScope™ Cluster
  • LifeScope™ Workstation
 
5. Translate color space to base space 
6. Visualize in context of annotation
  • LifeScope™ Genomic Analysis Software
  • LifeScope™ Cluster
  • LifeScope™ Workstation
7. Convert to SRF for publishing  
*** Need XSQ --> csfasta/qual converter

Learn more

Validate results using our BigDye® Direct Cycle Sequencing Kit, for Sanger sequencing on our capillary electrophoresis-based DNA sequencers.

BigDye® Direct Cycle Sequencing Kit

Decide on important experimental considerations: what type of variation you want to detect, in how many genes (or target regions), and how many samples?

Learn More:

Enrich your DNA using one of the following methods: PCR, long-range PCR, array hybridization, in-solution hybridization, or chromosome sorting. After enrichment, consider multiplexing strategies if you have multiple samples to process.

SequalPrep™ Long PCR kit and Normalization plate

Complete long PCR enrichment and amplicon normalization system that provides a highly efficient enrichment solution to meet NGS re-sequencing throughput capabilities.

Learn More:

Learn More:

User documentation for the 5500 Series Genetic Analysis Systems can be found on the SOLiD Community. Access is through the 5500 Series User Hub. Access is limited to 5500 Series Genetic Analysis Systems users who are registered on the solid community and have been authenticated through the process of requesting permission to join the 5500 Series User Hub.

The tools you need for each step in the targeted sequencing data analysis workflow:

 
Data Analysis StepApplied Biosystems Software3rd-Party Software***
1. Align reads to reference in color space
  • ICS
  • LifeScope™ Genomic Analysis Software
  • LifeScope™ Cluster
  • LifeScope™ Workstation
 
2. Generate quality metrics and perform mate pair rescue
  • LifeScope™ Genomic Analysis Software
  • LifeScope™ Cluster
  • LifeScope™ Workstation
 
3. Generate sequencing and alignment statistics
  • LifeScope™ Genomic Analysis Software
  • LifeScope™ Cluster
  • LifeScope™ Workstation
 
4. Identify Polymorphisms
  • LifeScope™ Genomic Analysis Software
  • LifeScope™ Cluster
  • LifeScope™ Workstation
 
5. Translate color space to base space 
6. Visualize in context of annotation
  • LifeScope™ Genomic Analysis Software
  • LifeScope™ Cluster
  • LifeScope™ Workstation
7. Convert to SRF for publishing  
*** Need XSQ --> csfasta/qual converter

Learn more

Validate results using our BigDye® Direct Cycle Sequencing Kit, for Sanger sequencing on our capillary electrophoresis-based DNA sequencers.

BigDye® Direct Cycle Sequencing Kit

Application Notes

Posters

Publications

For Research Use Only. Not for use in diagnostic procedures.