Search Thermo Fisher Scientific
Search Thermo Fisher Scientific
Invitrogen™ Lipofectamine™ LTX Reagent is a proprietary, animal-origin free formulation for the transfection of DNA into eukaryotic cells with low cytotoxicity. This reference provides a recommended procedure to transfect plasmid DNA into HEK 293, human embryonic kidney cells (ATCC No. CRL-1573) using Lipofectamine LTX Reagent.
Important guidelines for transfection
Follow these important guidelines when transfecting HEK 293 cells using Lipofectamine LTX Reagent:
Materials needed
Have the following reagents on hand before beginning:
Culture vessel | Surface area per well | Volume plating medium | Cells per well | Volume dilution medium | DNA | Lipofectamine LTX Reagent |
---|---|---|---|---|---|---|
96-well | 0.3 cm2 | 100 μl | 2.5 x 104 | 20 μl | 100 ng | 0.15 - 0.35 μl |
48-well | 1 cm2 | 200 μl | 5 x 104 | 40 μl | 200 ng | 0.30 - 0.7 μl |
24-well | 2 cm2 | 500 μl | 1.25 x 105 | 100 μl | 500 ng | 0.75 - 1.75 μl |
12-well | 4 cm2 | 1 ml | 2.5 x 105 | 200 μl | 1 μg | 1.5 - 3.5 μl |
6-well | 10 cm2 | 2 ml | 6.25 x 105 | 500 μl | 2.5 μg | 3.75 - 8.75 μl |