Organism TypeHamsterThe CHO-K1 cell line was derived as a subclone from the parental CHO cell line initiated from a biopsy of an ovary of an adult Chinese hamster by T. T. Puck in 1957. The cells require proline in the medium for growth. ATCC CCL-61
TissuesOvary
PhenotypeAdherent
Primaryno
ApplicationCell Culture / Growth Conditions,Protein Expression,Stable Cell Transfection,Transfection: Gene Expression,Transient Transfection

Recommended Transfection Reagents

  • Plasmid DNA transfection and co-transfection: Lipofectamine® 3000 (51-79% transfection efficiency and 1 fold protein expression improvement over Lipofectamine® 2000)
  • RNAi transfection (siRNA/miRNA) and gene silencing: Lipofectamine® RNAiMAX