Technical Resources

G-5 Supplement (100X) liquid

For the growth and expression of glial cells (normal and tumor) of astrocytic phenotype.

G-5 Supplement is a chemically defined, 100X concentrate of Bottenstein G-5 formulation (1). This Supplement is designed for the growth and expression of primary glial cells(astrocytes) or tumor cell lines of astrocytic phenotype(astrocytes and gliomas). It can be used for continuous cell lines. It can be used as a substitute for the G-2 Bottenstein formulation.

ComponentsMolecular WeightConcentration (mg/L)mM
Vitamins
Biotin244.0100.00.40983605
Proteins
Basic FGF0.5Infinity
EGF1.0Infinity
Human Transferrin5000.0Infinity
Insulin500.0Infinity
Other Components
Hydrocortisone362.470.369.931857E-4
Selenite173.00.520.0030057803

Reference:

1. Bottenstein, J.E. (1985) Cell Culture in the Neurosciences, Bottenstein, J.E. and Harvey, A.L., editors, p. 3, Plenum Press: New York and London.

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