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FluoCells® prepared slide #3 (Cat. no. F24630) containing a 16 µm cryostat section of mouse kidney stained with green-fluorescent Alexa Fluor® 488 wheat germ agglutinin (Cat. no. W11261), red-orange–fluorescent Alexa Fluor® 568 phalloidin (Cat. no. A12380) and blue-fluorescent DAPI (Cat. no. D1306, D3571, D21490). The image represents an optical section obtained by simultaneous two-photon excitation of all three dyes at 797 nm using a Bio-Rad Radiance 2100 multi-photon microscope system. The image was acquired at the 2001 3-D Microscopy of Living Cells Course, University of British Columbia, Vancouver, Canada, by John Jordan, Bio-Rad Laboratories; and Iain Johnson, Life Technologies.
CA1 pyramidal neuron in a hippocampal slice filled with SBFI delivered from a patch pipette. Go ›
Two-photon excitation imaging of calcium influx in a CA1 pyramidal cell spine Go ›
HeLa cells transduced with Organelle Lights™ Mito-GFP. Go ›
Four-color staining of a muntjac cell with probes for cytoskeletal, nuclear and mitochondrial proteins Go ›
1% Agarose gel containing 16S and 23S ribosomal RNA (rRNA). SYBR® Green II RNA gel stain. Go ›
Bovine pulmonary artery endothelial cells (BPAEC). MitoTracker® Red CMXRos, SYTOX® Green nucleic acid stain, biotin-XX goat anti–mouse IgG antibody and Cascade Blue® NeutrAvidin biotin-binding protein. Go ›