Announcing EVOS FL Auto Software Revision 1.7
Version 1.7 fixes a few bugs related to focus, Z-stack and scan-modes. It also introduces a new autofocus algorithm, addition of a “Quick Scan” option, and an ability to zoom in on multi-vessel maps, including the Fluidigm C1 chip, for easier and more accurate focus beacon placements. Among new features is also an algorithm that reduces vignetting using the color camera. The scale bar has been enhanced with color- and end-bar options and the ability to directly measure tile-stitched images.
Download and review the Installation Instructions before updating your software.
New features
Image acquisition, analysis, and review
- The autofocus algorithm was optimized, resulting in a faster and more accurate autofocus mechanism.
- A “Quick Scan” button was added. When pressed it captures an image at the center of each well of the selected well plate. Each image is saved according to the Quick Save settings.
- Enabling calibration of the X, Y and Z positions of Fluidigm C1 and C1 HT chips, automatic placement of image capture beacons at all cell capture sites, the ability to zoom in on the vessel map and select/de-select beacon placements and to run the vessels in either scan or time-lapse modes. Note that the 800 capture sites of the C1 HT chip must be imaged by two separate scans of the 400 sites on the left and right sectors, respectively.
Scan Mode
- A background correction option for color image scans was added. When enabled the vignetting at the corners of the image is estimated at the beginning of the scan and the corresponding correction is applied to each image. The result is reduced vignetting with the color camera. The impact of the background correction is most noticeable in scan-mode.
- The scale bar function was enhanced with options for a black or white scale bar, a toggle of the length and end-bar displays. The display of the scale-bar in zoomed images was also improved.
- The ability to measure images captured in “Scan mode” was added.
- Scanned images are now shown with pseudo-coloring turned on rather than in black and white.
- A function was added to enable zooming in on multi-well vessel maps for more accurate well selection.
Bug fixes
General bug fixes
- A bug that potentially impacted white-balance was corrected.
Focus-related bug fixes
- Correction of a problem with Capture Focus Nominal for the Cy®5.5 cube.
Z-stack-related bug fixes
- The Z-stack code has been revised to include capture of the focus-nominal position. The saved file has the text “NOMINAL” appended to the file name.
Scan mode-related bug fixes
- A bug with editing beacon locations in a previous created scan routine has been fixed; the edited locations are now used.
- A bug that risked causing failures in when acquiring images in “Scan mode” with the color camera has been corrected.