Invitrogen

CD4 Monoclonal Antibody (SK3 (SK-3)), PE-Cyanine7, eBioscience™

4 Published Figures

12 References

View all (606) CD4 antibodies

Datasheet

Protocols

Questions & Answers

Datasheet

Protocols

Questions & Answers

Antibody Testing Data (1)
Published Figures (4)

FIGURE: 1 / 5

CD4 Antibody (25-0047-42) in Flow

Staining of normal human peripheral blood cells with Anti-Human CD8a FITC (Product # 11-0088-42) and Mouse IgG1 K Isotype Control PE-Cyanine7 (Product # 25-4714-80) (left) or Anti-Human CD4 PE-Cyanine7 (right). Cells in the lymphocyte gate were used for analysis.

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Product Details

25-0047-42

Applications

Tested Dilution

Publications

Flow Cytometry (Flow)

5 µL (0.06 µg)/test

View 12 publications 12 publications

Product Specifications

Species Reactivity

Human

Published species

Human

Host/Isotype

Mouse / IgG1, kappa

Recommended Isotype Control

Mouse IgG1 kappa Isotype Control (P3.6.2.8.1), PE-Cyanine7, eBioscience™

Class

Monoclonal

Type

Antibody

Clone

SK3 (SK-3)

Conjugate

PE-Cyanine7 PE-Cyanine7 PE-Cyanine7

Excitation/Emission Max

569/780 nm View spectra

Form

Liquid

Concentration

5 µL/Test

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.2, with 0.2% BSA

Contains

0.09% sodium azide

Storage conditions

4° C, store in dark, DO NOT FREEZE!

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_10547059

Product Specific Information

Description: The SK3 monoclonal antibody reacts with human CD4, a 59-kDa cell surface receptor expressed by a majority of thymocytes, a subpopulation of mature T helper cells, and at low levels on monocytes. CD4 is a receptor for the human immunodeficiency virus (HIV). SK3 blocks HIV binding and mixed lymphocyte reaction. The SK3 and RPA-T4 monoclonal antibodies do not cross-block binding, suggesting recognition of distinct epitopes.

Applications Reported: This SK3 (SK-3) antibody has been reported for use in flow cytometric analysis.

Applications Tested: This SK3 (SK-3) antibody has been pre-titrated and tested by flow cytometric analysis of normal human peripheral blood cells. This can be used at 5 µL (0.06 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test.

Light sensitivity: This tandem dye is sensitive photo-induced oxidation. Please protect this vial and stained samples from light.

Fixation: Samples can be stored in IC Fixation Buffer (Product # 00-822-49) (100 µL cell sample + 100 µL IC Fixation Buffer) or 1-step Fix/Lyse Solution (Product # 00-5333-54) for up to 3 days in the dark at 4°C with minimal impact on brightness and FRET efficiency/compensation. Some generalizations regarding fluorophore performance after fixation can be made, but clone specific performance should be determined empirically.

Excitation: 488-561 nm; Emission: 775 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser.

Filtration: 0.2 µm post-manufacturing filtered.

Target Information

The CD4 antigen is involved in the recognition of MHC class II molecules and is a co-receptor for HIV. CD4 is primarily expressed in a subset of T-lymphocytes, also referred to as T helper cells, but may also be expressed by other cells in the immune system, such as monocytes, macrophages, and dendritic cells. At the tissue level, CD4 expression may be detected in thymus, lymph nodes, tonsils, and spleen, and also in specific regions of the brain, gut, and other non-lymphoid tissues. CD4 functions to initiate or augment the early phase of T-cell activation through its association with the T-cell receptor complex and protein tyrosine kinase, Lck. It may also function as an important mediator of direct neuronal damage in infectious and immune-mediated diseases of the central nervous system. Multiple alternatively spliced transcripts have been identified in this gene [RefSeq, July 2017].

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Product References

A Novel Homozygous Stop Mutation in IL23R Causes Mendelian Susceptibility to Mycobacterial Disease.

Journal of clinical immunology

Staels F,Lorenzetti F,De Keukeleere K,Willemsen M,Gerbaux M,Neumann J,Tousseyn T,Pasciuto E,De Munter P,Bossuyt X,Gijsbers R,Liston A,Humblet-Baron S,Schrijvers R

25-0047-42 was used in Flow cytometry/Cell sorting to demonstrate that impaired IL-23 immunity caused by a homozygous R381X mutation in IL23R underlies MSMD, corroborating earlier findings with a homozygous p.C115Y IL23R mutation.

Tue Nov 01 00:00:00 EDT 2022

SPARC regulation of PMN clearance protects from pristane-induced lupus and rheumatoid arthritis.

iScience

Sangaletti S,Botti L,Gulino A,Lecis D,Bassani B,Portararo P,Milani M,Cancila V,De Cecco L,Dugo M,Tripodo C,Colombo MP

Published figure using CD4 monoclonal antibody (Product # 25-0047-42) in Flow Cytometry

Fri Jun 25 00:00:00 EDT 2021

Human Mast Cell Proteome Reveals Unique Lineage, Putative Functions, and Structural Basis for Cell Ablation.

Immunity

Plum T,Wang X,Rettel M,Krijgsveld J,Feyerabend TB,Rodewald HR

25-0047 was used in Flow cytometry/Cell sorting to identify a mast-cell-specific proteome signature, indicative of a unique lineage, only distantly related to other immune cell types, including innate immune cells.

Tue Feb 18 00:00:00 EST 2020

Interleukin-6-knockdown of chimeric antigen receptor-modified T cells significantly reduces IL-6 release from monocytes.

Experimental hematology & oncology

Kang L,Tang X,Zhang J,Li M,Xu N,Qi W,Tan J,Lou X,Yu Z,Sun J,Wang Z,Dai H,Chen J,Lin G,Wu D,Yu L

25-0047 was used in Flow cytometry/Cell sorting to investigate whether IL-6 knockdown in CART-19 cells can reduce IL-6 secretion from monocytes, which may reduce the risk of adverse events.

Fri Apr 15 00:00:00 EDT 2022

Interleukin-6-knockdown of chimeric antigen receptor-modified T cells significantly reduces IL-6 release from monocytes.

Experimental hematology & oncology

Kang L,Tang X,Zhang J,Li M,Xu N,Qi W,Tan J,Lou X,Yu Z,Sun J,Wang Z,Dai H,Chen J,Lin G,Wu D,Yu L

25-0047 was used in Flow cytometry/Cell sorting to investigate whether IL-6 knockdown in CART-19 cells can reduce IL-6 secretion from monocytes, which may reduce the risk of adverse events.

Fri Apr 15 00:00:00 EDT 2022

Impaired lymph node stromal cell function during the earliest phases of rheumatoid arthritis.

Arthritis research & therapy

Hähnlein JS,Nadafi R,de Jong T,Ramwadhdoebe TH,Semmelink JF,Maijer KI,Zijlstra IA,Maas M,Gerlag DM,Geijtenbeek TBH,Tak PP,Mebius RE,van Baarsen LGM

25-0047 was used in Flow cytometry/Cell sorting to develop an experimental model to allow the research on the role of lymph node stromal cells during the earliest phases of rheumatoid arthritis.

Mon Feb 26 00:00:00 EST 2018

Characteristic patterns of HLA presentation and T cell differentiation in adult-onset Still's disease.

International journal of immunopathology and pharmacology

Jung JY,Choi B,Sayeed HM,Suh CH,Kim YW,Kim HA,Sohn S

Published figure using CD4 monoclonal antibody (Product # 25-0047-42) in Flow Cytometry

Tue Dec 18 00:00:00 EST 2018

HDAC inhibition potentiates immunotherapy in triple negative breast cancer.

Oncotarget

Terranova-Barberio M,Thomas S,Ali N,Pawlowska N,Park J,Krings G,Rosenblum MD,Budillon A,Munster PN

Published figure using CD4 monoclonal antibody (Product # 25-0047-42) in Flow Cytometry

Tue Dec 26 00:00:00 EST 2017

Alloantigen-specific regulatory T cells generated with a chimeric antigen receptor.

The Journal of clinical investigation

MacDonald KG,Hoeppli RE,Huang Q,Gillies J,Luciani DS,Orban PC,Broady R,Levings MK

Published figure using CD4 monoclonal antibody (Product # 25-0047-42) in Flow Cytometry

Fri Apr 01 00:00:00 EDT 2016

Evidence for Resident Memory T Cells in Rasmussen Encephalitis.

Frontiers in immunology

Owens GC,Chang JW,Huynh MN,Chirwa T,Vinters HV,Mathern GW

25-0047 was used in Flow cytometry/Cell sorting to demonstrate that a cellular immune response to an as yet unidentified antigen(s) occurs at an early stage of Rasmussen encephalitis.

Fri Mar 04 00:00:00 EST 2016

CIB1 and CIB2 are HIV-1 helper factors involved in viral entry.

Scientific reports

Godinho-Santos A,Hance AJ,Gonçalves J,Mammano F

Published figure using CD4 monoclonal antibody (Product # 25-0047-42) in Flow Cytometry

Thu Aug 04 00:00:00 EDT 2016

Long-lasting multifunctional CD8⁺ T cell responses in end-stage melanoma patients can be induced by dendritic cell vaccination.

Oncoimmunology

Wimmers F,Aarntzen EH,Duiveman-deBoer T,Figdor CG,Jacobs JF,Tel J,de Vries IJ

25-0047 was used in Flow cytometry/Cell sorting to investigate the functionality of tumour-specific CD8(+) T cells in melanoma patients upon dendritic cell vaccination, showing long-lasting multifunctional responses in end-stage patients.

Sat Jan 29 00:00:00 EST 2022

Progression of Lung Cancer Is Associated with Increased Dysfunction of T Cells Defined by Coexpression of Multiple Inhibitory Receptors.

Cancer immunology research

Thommen DS,Schreiner J,Müller P,Herzig P,Roller A,Belousov A,Umana P,Pisa P,Klein C,Bacac M,Fischer OS,Moersig W,Savic Prince S,Levitsky V,Karanikas V,Lardinois D,Zippelius A

25-0047 was used in Flow cytometry/Cell sorting to characterise the diversity and expression patterns of inhibitory receptors on tumour-infiltrating T cells from patients with non-small cell lung cancer.

Tue Dec 01 00:00:00 EST 2015

Bioinformatics

Protein Aliases: CD4; CD4 antigen (p55); CD4 antigen p55; CD4 receptor; cd4a; fCD4; Leu-3; T-cell surface antigen T4/Leu-3; T-cell surface glycoprotein CD4

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Gene Aliases: CD4; CD4mut

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UniProt ID: (Human) P01730

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Entrez Gene ID: (Human) 920

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Function(s)

virus receptor activity glycoprotein binding receptor activity transmembrane signaling receptor activity extracellular matrix structural constituent protein binding zinc ion binding coreceptor activity immunoglobulin binding enzyme binding protein kinase binding MHC class II protein binding protein homodimerization activity protein tyrosine kinase binding

Process(es)

cytokine production adaptive immune response induction by virus of host cell-cell fusion immune response signal transduction cell surface receptor signaling pathway enzyme linked receptor protein signaling pathway transmembrane receptor protein tyrosine kinase signaling pathway positive regulation of calcium ion transport into cytosol fusion of virus membrane with host plasma membrane T cell differentiation entry into host cell T cell costimulation response to estradiol maintenance of protein location in cell response to vitamin D helper T cell enhancement of adaptive immune response positive regulation of T cell proliferation T cell selection positive regulation of interleukin-2 biosynthetic process positive regulation of protein kinase activity regulation of defense response to virus by virus positive regulation of peptidyl-tyrosine phosphorylation defense response to Gram-negative bacterium positive regulation of calcium-mediated signaling T cell receptor signaling pathway regulation of T cell activation

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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CD4 Monoclonal Antibody (SK3 (SK-3)), PE-Cyanine7, eBioscience™

CD4 Antibody (25-0047-42) in Flow

Product Details

25-0047-42

Flow Cytometry (Flow)

Species Reactivity

Published species

Host/Isotype

Recommended Isotype Control

Class

Type

Clone

Conjugate

Excitation/Emission Max

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

How to use the Panel Builder

Bioinformatics

Performance Guarantee

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