Invitrogen

CD4 Monoclonal Antibody (SK3 (SK-3)), PE-Cyanine5.5, eBioscience™

FIGURE: 1 / 5

CD4 Antibody (35-0047-42) in Flow

Staining of normal human peripheral blood cells with Anti-Human CD8a FITC (Product # 11-0088-42) and Mouse IgG1 K Isotype Control PE-Cyanine5-5 (Product # 35-4714-82) (left) or Anti-Human CD4 PE-Cyanine5-5 (right). Cells in the lymphocyte gate were used for analysis.

Product Image Gallery

Product Details

35-0047-42

Applications

Tested Dilution

Publications

Flow Cytometry (Flow)

5 µL (0.125 µg)/test

View 9 publications 9 publications

Product Specifications

Species Reactivity

Human

Published species

Human

Host/Isotype

Mouse / IgG1, kappa

Recommended Isotype Control

Mouse IgG1 kappa Isotype Control (P3.6.2.8.1), PE-Cyanine5.5, eBioscience™

Class

Monoclonal

Type

Antibody

Clone

SK3 (SK-3)

Conjugate

PE-Cyanine5.5 PE-Cyanine5.5 PE-Cyanine5.5

Excitation/Emission Max

564/692 nm View spectra

Form

Liquid

Concentration

5 µL/Test

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.2, with 0.2% BSA

Contains

0.09% sodium azide

Storage conditions

4° C, store in dark, DO NOT FREEZE!

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_11218283

Product Specific Information

Description: The SK3 monoclonal antibody reacts with human CD4, a 59-kDa cell surface receptor expressed by a majority of thymocytes, a subpopulation of mature T helper cells, and at low levels on monocytes. CD4 is a receptor for the human immunodeficiency virus (HIV). SK3 blocks HIV binding and mixed lymphocyte reaction. The SK3 and RPA-T4 monoclonal antibodies do not cross-block binding, suggesting recognition of distinct epitopes.

Applications Reported: This SK3 (SK-3) antibody has been reported for use in flow cytometric analysis.

Applications Tested: This SK3 (SK-3) antibody has been pre-titrated and tested by flow cytometric analysis of normal human peripheral blood cells. This can be used at 5 µL (0.125 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test.

Light sensitivity: This tandem dye is sensitive photo-induced oxidation. Please protect this vial and stained samples from light.

Fixation: Samples can be stored in IC Fixation Buffer (Product # 00-822-49) (100 µL cell sample + 100 µL IC Fixation Buffer) or 1-step Fix/Lyse Solution (Product # 00-5333-54) for up to 3 days in the dark at 4°C with minimal impact on brightness and FRET efficiency/compensation. Some generalizations regarding fluorophore performance after fixation can be made, but clone specific performance should be determined empirically.

Excitation: 488-561 nm; Emission: 695 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser.

Target Information

The CD4 antigen is involved in the recognition of MHC class II molecules and is a co-receptor for HIV. CD4 is primarily expressed in a subset of T-lymphocytes, also referred to as T helper cells, but may also be expressed by other cells in the immune system, such as monocytes, macrophages, and dendritic cells. At the tissue level, CD4 expression may be detected in thymus, lymph nodes, tonsils, and spleen, and also in specific regions of the brain, gut, and other non-lymphoid tissues. CD4 functions to initiate or augment the early phase of T-cell activation through its association with the T-cell receptor complex and protein tyrosine kinase, Lck. It may also function as an important mediator of direct neuronal damage in infectious and immune-mediated diseases of the central nervous system. Multiple alternatively spliced transcripts have been identified in this gene [RefSeq, July 2017].

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

How to use the Panel Builder

Watch the video to learn how to use the Invitrogen Flow Cytometry Panel Builder to build your next flow cytometry panel in 5 easy steps.

Product References

On Immunological Studies at Sirius University of Science and Technology.

Molecular biology

Astrakhantseva IV,Krut' VG,Chuvpilo SA,Shevyrev DV,Shumeev AN,Rybtsov SA,Nedospasov SA

35-0047-42 was used in Flow cytometry/Cell sorting to summarize the results of recent immunological studies performed at new Sirius University of Science and Technology.

Wed May 03 00:00:00 EDT 2023

SPARC regulation of PMN clearance protects from pristane-induced lupus and rheumatoid arthritis.

iScience

Sangaletti S,Botti L,Gulino A,Lecis D,Bassani B,Portararo P,Milani M,Cancila V,De Cecco L,Dugo M,Tripodo C,Colombo MP

Published figure using CD4 monoclonal antibody (Product # 35-0047-42) in Flow Cytometry

Fri Jun 25 00:00:00 EDT 2021

Activation of Bone Marrow Adaptive Immunity in Type 2 Diabetes: Rescue by Co-stimulation Modulator Abatacept.

Frontiers in immunology

Santopaolo M,Sullivan N,Thomas AC,Alvino VV,Nicholson LB,Gu Y,Spinetti G,Kallikourdis M,Blom A,Madeddu P

35-0047-42 was used in Flow Cytometry to identify the role of Abatacept in reducing the demonstrated T cell activation in the bone marrow of type 2 diabetes patients and mice.

Fri Jul 02 00:00:00 EDT 2021

Th2 Biased Immunity With Altered B Cell Profiles in Circulation of Patients With Sporotrichosis Caused by Sporothrix globosa.

Frontiers in immunology

Zu J,Yao L,Song Y,Cui Y,Guan M,Chen R,Zhen Y,Li S

35-0047 was used in Flow cytometry/Cell sorting to shed light on the potential involvement of peripheral T and B cell immunity against this mycotic infection.

Mon May 31 00:00:00 EDT 2021

Interleukin-6-knockdown of chimeric antigen receptor-modified T cells significantly reduces IL-6 release from monocytes.

Experimental hematology & oncology

Kang L,Tang X,Zhang J,Li M,Xu N,Qi W,Tan J,Lou X,Yu Z,Sun J,Wang Z,Dai H,Chen J,Lin G,Wu D,Yu L

Published figure using CD4 monoclonal antibody (Product # 35-0047-42) in Flow Cytometry

Fri Apr 15 00:00:00 EDT 2022

Characteristic patterns of HLA presentation and T cell differentiation in adult-onset Still's disease.

International journal of immunopathology and pharmacology

Jung JY,Choi B,Sayeed HM,Suh CH,Kim YW,Kim HA,Sohn S

Published figure using CD4 monoclonal antibody (Product # 35-0047-42) in Flow Cytometry

Tue Dec 18 00:00:00 EST 2018

HDAC inhibition potentiates immunotherapy in triple negative breast cancer.

Oncotarget

Terranova-Barberio M,Thomas S,Ali N,Pawlowska N,Park J,Krings G,Rosenblum MD,Budillon A,Munster PN

Published figure using CD4 monoclonal antibody (Product # 35-0047-42) in Flow Cytometry

Tue Dec 26 00:00:00 EST 2017

Alloantigen-specific regulatory T cells generated with a chimeric antigen receptor.

The Journal of clinical investigation

MacDonald KG,Hoeppli RE,Huang Q,Gillies J,Luciani DS,Orban PC,Broady R,Levings MK

Published figure using CD4 monoclonal antibody (Product # 35-0047-42) in Flow Cytometry

Fri Apr 01 00:00:00 EDT 2016

CIB1 and CIB2 are HIV-1 helper factors involved in viral entry.

Scientific reports

Godinho-Santos A,Hance AJ,Gonçalves J,Mammano F

Published figure using CD4 monoclonal antibody (Product # 35-0047-42) in Flow Cytometry

Thu Aug 04 00:00:00 EDT 2016

Bioinformatics

Protein Aliases: CD4; CD4 antigen (p55); CD4 antigen p55; CD4 receptor; cd4a; fCD4; Leu-3; T-cell surface antigen T4/Leu-3; T-cell surface glycoprotein CD4

View more View less

Gene Aliases: CD4; CD4mut

View more View less

UniProt ID: (Human) P01730

View more View less

Entrez Gene ID: (Human) 920

View more View less

Function(s)

virus receptor activity glycoprotein binding receptor activity transmembrane signaling receptor activity extracellular matrix structural constituent protein binding zinc ion binding coreceptor activity immunoglobulin binding enzyme binding protein kinase binding MHC class II protein binding protein homodimerization activity protein tyrosine kinase binding

Process(es)

cytokine production adaptive immune response induction by virus of host cell-cell fusion immune response signal transduction cell surface receptor signaling pathway enzyme linked receptor protein signaling pathway transmembrane receptor protein tyrosine kinase signaling pathway positive regulation of calcium ion transport into cytosol fusion of virus membrane with host plasma membrane T cell differentiation entry into host cell T cell costimulation response to estradiol maintenance of protein location in cell response to vitamin D helper T cell enhancement of adaptive immune response positive regulation of T cell proliferation T cell selection positive regulation of interleukin-2 biosynthetic process positive regulation of protein kinase activity regulation of defense response to virus by virus positive regulation of peptidyl-tyrosine phosphorylation defense response to Gram-negative bacterium positive regulation of calcium-mediated signaling T cell receptor signaling pathway regulation of T cell activation

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

Learn more

CD4 Monoclonal Antibody (SK3 (SK-3)), PE-Cyanine5.5, eBioscience™

CD4 Antibody (35-0047-42) in Flow

Product Details

35-0047-42

Flow Cytometry (Flow)

Species Reactivity

Published species

Host/Isotype

Recommended Isotype Control

Class

Type

Clone

Conjugate

Excitation/Emission Max

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

How to use the Panel Builder

Bioinformatics

Performance Guarantee

We're here to help

Your items have has been added!