Invitrogen

EpCAM Monoclonal Antibody (VU-1D9)

Advanced Verification

This Antibody was verified by Relative expression to ensure that the antibody binds to the antigen stated.

12 References

View all (275) EpCAM (CD326) antibodies

Datasheet

Protocols

Questions & Answers

Datasheet

Protocols

Questions & Answers

Antibody Testing Data (5)
Advanced Verification (1)

EpCAM Antibody in Immunocytochemistry (ICC/IF)

FIGURE: 1 / 6

EpCAM Antibody (MA1-10195) in ICC/IF

Immunofluorescence analysis of EpCAM was performed using HT-29 and HeLa cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 2% BSA for 1 hour at room temperature. The cells were labeled with EpCAM Mouse Monoclonal Antibody (Product # MA1-10195) at 1 µg/mL in 0.1% BSA and incubated overnight at 4 degree and then labeled with Goat anti-Mouse IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alexa Fluor Plus 488 (Product # A32723) at a dilution of 1:2000... View More

EpCAM Antibody in Immunohistochemistry (Paraffin) (IHC (P))

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EpCAM Antibody — Antibody specificity was demonstrated by detection of differential basal expression of the target across cell lines tested owing to their inherent genetic constitution. Relative expression of EpCAM was observed in HT-29 cells in plasma membrane, which is a positive model for EpCAM expression, in comparison to HeLa (null for EpCAM expression) using Anti-EpCAM Mouse Monoclonal Antibody (Product # MA1-10195) in immunofluorescence. {RE}

Product Details

MA1-10195

Applications

Tested Dilution

Publications

Western Blot (WB)

1-4 µg/mL

View 3 publications 3 publications

Immunohistochemistry (IHC)

View 6 publications 6 publications

Immunohistochemistry (Paraffin) (IHC (P))

5-10 µg/mL

Immunohistochemistry (Frozen) (IHC (F))

Assay-dependent

Immunocytochemistry (ICC/IF)

4 µg/mL

View 2 publications 2 publications

Flow Cytometry (Flow)

1-3 µg/mL

View 1 publication 1 publication

Immunoprecipitation (IP)

1-2 µg/mL

Product Specifications

Species Reactivity

Human

Published species

Human

Host/Isotype

Mouse / IgG1

Class

Monoclonal

Type

Antibody

Clone

VU-1D9

Immunogen

Small cell lung carcinoma cell line H69.

View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Protein A

Storage buffer

PBS, pH 7.4

Contains

15mM sodium azide

Storage conditions

4° C, do not freeze

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_11153547

Product Specific Information

This antibody recognizes an extracellular epitope within EGF-like domain I of CD326 / EpCAM, a marker of epithelial lineages. This antibody strongly stains various normal epithelial cells and carcinomas.

Immunohistochemistry (Paraffin): Antigen retrieval: heat (sodium citrate) + trypsin.

Target Information

Ep-CAM (epithelial adhesion molecule, epithelial specific antigen, ESA) is a transmembrane glycoprotein expressed in the epithelium with a molecular weight of approximately 40 kDa, which functions as an epithelial cell adhesion molecule. Ep-CAM functions as a homotypic calcium-independent cell adhesion molecule, and has a direct impact on cell cycle, proliferation and metabolism of epithelial cells and fibroblasts due to its ability to rapidly induce the proto-oncogene c-myc and the cell cycle regulating genes cyclin A and E. Ep-CAM mediates Ca2+-independent homotypic interactions. Formation of Ep-CAM-mediated adhesions have a negative regulatory effect on adhesions mediated by classic cadherins, which may have strong effects on the differentiation and growth of epithelial cells. Ep-CAM overexpression was suggested to be associated with enhanced epithelial proliferation. Ep-CAM is highly expressed in human carcinomas, and is a marker for tumors of epithelial lineage. Ep-CAM is expressed on baso-lateral cell surface in most simple epithelia and many carcinoma types. Also, Ep-CAM reportedly distinguishes adenocarcinomas from pleural mesotheliomas.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Stable expansion of high-grade serous ovarian cancer organoids requires a low-Wnt environment.

The EMBO journal

Hoffmann K,Berger H,Kulbe H,Thillainadarasan S,Mollenkopf HJ,Zemojtel T,Taube E,Darb-Esfahani S,Mangler M,Sehouli J,Chekerov R,Braicu EI,Meyer TF,Kessler M

MA1-10195 was used in Immunohistochemistry to illustrate that a low-Wnt enviorment coupled with active BMP signaling are required for generation and sustenance of HGSOC organoids.

Mon Mar 16 00:00:00 EDT 2020

Widespread GLI expression but limited canonical hedgehog signaling restricted to the ductular reaction in human chronic liver disease.

PloS one

Grzelak CA,Sigglekow ND,Tirnitz-Parker JE,Hamson EJ,Warren A,Maneck B,Chen J,Patkunanathan B,Boland J,Cheng R,Shackel NA,Seth D,Bowen DG,Martelotto LG,Watkins DN,McCaughan GW

MA110195 was used in immunohistochemistry to analyze the importance of canonical Hedgehog signaling within the primary cilia+ liver progenitor cell population in humans

Wed Aug 09 00:00:00 EDT 2017

Optimisation of immunofluorescence methods to determine MCT1 and MCT4 expression in circulating tumour cells.

BMC cancer

Kershaw S,Cummings J,Morris K,Tugwood J,Dive C

MA1-10195 was used in western blot to develop an immunofluorescence method to assess MCT1 and MCT4 in circulating tumor cells and as them as biomarkers of AZD3965 in cancer patients

Sun May 10 00:00:00 EDT 2015

New frontiers in circulating tumor cell analysis: A reference guide for biomolecular profiling toward translational clinical use.

International journal of cancer

Becker TM,Caixeiro NJ,Lim SH,Tognela A,Kienzle N,Scott KF,Spring KJ,de Souza P

MA1-10195 was used in immunocytochemistry to review the current state-of-the-art in circulating tumor cell profiling

Sun Jun 01 00:00:00 EDT 2014

Neuropilin-2 Is upregulated in lung cancer cells during TGF-β1-induced epithelial-mesenchymal transition.

Cancer research

Nasarre P,Gemmill RM,Potiron VA,Roche J,Lu X,Barón AE,Korch C,Garrett-Mayer E,Lagana A,Howe PH,Drabkin HA

MA1-10195 was used in western blot to study TGF-beta1-driven lung cancer cell epithelial-to-mesenchymal transition and the role played by neuropilin-2

Sun Dec 01 00:00:00 EST 2013

Systematic analysis and validation of differential gene expression in ovarian serous adenocarcinomas and normal ovary.

Journal of cancer research and clinical oncology

Bauerschlag D,Bräutigam K,Moll R,Sehouli J,Mustea A,Salehin D,Krajewska M,Reed JC,Maass N,Hampton GM,Meinhold-Heerlein I

MA1-10195 was used in immunohistochemistry to identify genes differentially expressed in ovaraian serous adenocarcinoma as compared to healthy ovarian tissue

Fri Feb 01 00:00:00 EST 2013

Multiple breast cancer cell-lines derived from a single tumor differ in their molecular characteristics and tumorigenic potential.

PloS one

Mosoyan G,Nagi C,Marukian S,Teixeira A,Simonian A,Resnick-Silverman L,DiFeo A,Johnston D,Reynolds SR,Roses DF,Mosoian A

MA1-10195 was used in flow cytometry to study the different molecular phenotype and tumorigenicity of 5 breast cancer cell lines derived from a single patient

Fri Jul 26 00:00:00 EDT 2013

A pilot study to explore circulating tumour cells in pancreatic cancer as a novel biomarker.

British journal of cancer

Khoja L,Backen A,Sloane R,Menasce L,Ryder D,Krebs M,Board R,Clack G,Hughes A,Blackhall F,Valle JW,Dive C

MA1-10195 was used in immunocytochemistry and immunohistochemistry - paraffin section to investigate epithelial to mesenchymal transition during metastasis of pancreatic cancer

Tue Jan 31 00:00:00 EST 2012

Transient but not stable ZEB1 knockdown dramatically inhibits growth of malignant pleural mesothelioma cells.

Annals of surgical oncology

Horio M,Sato M,Takeyama Y,Elshazley M,Yamashita R,Hase T,Yoshida K,Usami N,Yokoi K,Sekido Y,Kondo M,Toyokuni S,Gazdar AF,Minna JD,Hasegawa Y

MA1-10195 was used in western blot to study the role of ZEB1 in mediating the growth of malignant pleural mesothelioma cells

Sun Jul 01 00:00:00 EDT 2012

Heritable somatic methylation and inactivation of MSH2 in families with Lynch syndrome due to deletion of the 3' exons of TACSTD1.

Nature genetics

Ligtenberg MJ,Kuiper RP,Chan TL,Goossens M,Hebeda KM,Voorendt M,Lee TY,Bodmer D,Hoenselaar E,Hendriks-Cornelissen SJ,Tsui WY,Kong CK,Brunner HG,van Kessel AG,Yuen ST,van Krieken JH,Leung SY,Hoogerbrugge N

MA1-10195 was used in immunohistochemistry to investigate Lynch syndrome with MSH2-deficient tumors

Thu Jan 01 00:00:00 EST 2009

Molecular and prognostic distinction between serous ovarian carcinomas of varying grade and malignant potential.

Oncogene

Meinhold-Heerlein I,Bauerschlag D,Hilpert F,Dimitrov P,Sapinoso LM,Orlowska-Volk M,Bauknecht T,Park TW,Jonat W,Jacobsen A,Sehouli J,Luttges J,Krajewski M,Krajewski S,Reed JC,Arnold N,Hampton GM

MA1-10195 was used in immunohistochemistry to distinguish low malignant potential serous ovarian carcinomas from invasive counterparts

Thu Feb 03 00:00:00 EST 2005

Gene expression patterns in ovarian carcinomas.

Molecular biology of the cell

Schaner ME,Ross DT,Ciaravino G,Sorlie T,Troyanskaya O,Diehn M,Wang YC,Duran GE,Sikic TL,Caldeira S,Skomedal H,Tu IP,Hernandez-Boussard T,Johnson SW,O'Dwyer PJ,Fero MJ,Kristensen GB,Borresen-Dale AL,Hastie T,Tibshirani R,van de Rijn M,Teng NN,Longacre TA,Botstein D,Brown PO,Sikic BI

MA1-10195 was used in immunohistochemistry to study gene expression patterns in ovarian carcinomas using DNA microarrays

Sat Nov 01 00:00:00 EST 2003

Bioinformatics

Protein Aliases: Adenocarcinoma-associated antigen; CD326; Cell surface glycoprotein Trop-1; EGP; EGP314; Ep-CAM; Epithelial cell adhesion molecule; Epithelial cell surface antigen; Epithelial glycoprotein; Epithelial glycoprotein 314; hEGP314; human epithelial glycoprotein-2; KS 1/4 antigen; KSA; Major gastrointestinal tumor-associated protein GA733-2; membrane component, chromosome 4, surface marker (35kD glycoprotein); Tumor-associated calcium signal transducer 1

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Gene Aliases: DIAR5; EGP-2; EGP314; EGP40; EPCAM; ESA; GA733-2; HNPCC8; KS1/4; KSA; M1S2; M4S1; MIC18; MK-1; TACSTD1; TROP1

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UniProt ID: (Human) P16422

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Entrez Gene ID: (Human) 4072

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Function(s)

protein binding protein complex binding cadherin binding involved in cell-cell adhesion

Process(es)

ureteric bud development positive regulation of cell proliferation signal transduction involved in regulation of gene expression negative regulation of apoptotic process positive regulation of transcription from RNA polymerase II promoter stem cell differentiation cell-cell adhesion negative regulation of cell-cell adhesion mediated by cadherin positive regulation of cell motility positive regulation of stem cell proliferation

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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EpCAM Monoclonal Antibody (VU-1D9)

EpCAM Antibody (MA1-10195) in ICC/IF

Product Details

MA1-10195

Western Blot (WB)

Immunohistochemistry (IHC)

Immunohistochemistry (Paraffin) (IHC (P))

Immunohistochemistry (Frozen) (IHC (F))

Immunocytochemistry (ICC/IF)

Flow Cytometry (Flow)

Immunoprecipitation (IP)

Species Reactivity

Published species

Host/Isotype

Class

Type

Clone

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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