Invitrogen

CD11a (LFA-1alpha) Monoclonal Antibody (M17/4), FITC, eBioscience™

CD11a (LFA-1alpha) Antibody in Flow Cytometry (Flow)

FIGURE: 1 / 4

CD11a (LFA-1alpha) Antibody (11-0111-82) in Flow

Staining of BALB/c splenocytes with 0.25 µg of Rat IgG2a K Isotype Control FITC (Product # 11-4321-42) (blue histogram) or 0.25 µg of Anti-Mouse CD11a FITC (purple histogram). Cells in the lymphocyte gate were used for analysis.

CD11a (LFA-1alpha) Antibody in Immunohistochemistry (IHC)

Product Image Gallery

Product Details

11-0111-82

Applications

Tested Dilution

Publications

Immunohistochemistry (IHC)

View 1 publication 1 publication

Flow Cytometry (Flow)

0.5 µg/test

View 24 publications 24 publications

Product Specifications

Species Reactivity

Mouse

Published species

Human, Mouse

Host/Isotype

Rat / IgG2a, kappa

Recommended Isotype Control

Rat IgG2a kappa Isotype Control (eBR2a), FITC, eBioscience™

Class

Monoclonal

Type

Antibody

Clone

M17/4

Conjugate

FITC FITC FITC

Excitation/Emission Max

498/517 nm View spectra

Form

Liquid

Concentration

0.5 mg/mL

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.2

Contains

0.09% sodium azide

Storage conditions

4° C, store in dark, DO NOT FREEZE!

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_464931

Product Specific Information

Description: The M17/4 monoclonal antibody reacts with mouse CD11a, the 180 kDa integrin alpha L, also known as the lymphocyte function associated antigen-1 (LFA-1) alpha chain. LFA-1, formed by non-covalent association of CD11a with CD18 (integrin beta 2), serves as an important adhesion molecule involved in lymphocyte and granulocyte function. CD54 (ICAM-1), CD102 (ICAM-2), and CD50 (ICAM-3) are ligands for LFA-1. CD11a is expressed by all leukocytes.

Applications Reported: The M17/4 antibody has been reported for use in flow cytometric analysis.

Applications Tested: The M17/4 antibody has been tested by flow cytometric analysis of mouse splenocytes. This can be used at less than or equal to 0.5 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

Excitation: 488 nm; Emission: 520 nm; Laser: Blue Laser.

Filtration: 0.2 µm post-manufacturing filtered.

Target Information

CD11a (LFA-1 alpha, ITGAL) together with CD18 constitute leukocyte function-associated antigen 1 (LFA-1), the alphaLbeta2 integrin. CD11a is implicated in activation of LFA-1 complex. CD11a plays a central role in leukocyte intercellular adhesion through interactions with its ligands, ICAMs 1-3 (intercellular adhesion molecules 1 through 3), and also functions in lymphocyte costimulatory signaling. CD11a is expressed on the plasma membrane of leukocytes in a low-affinity conformation. Cell stimulation by chemokines or other signals leads to induction the high-affinity conformation, which supports tight binding of CD11a to its ligands, the intercellular adhesion molecules ICAM-1, -2, -3. CD11a is thus involved in interaction of various immune cells and in their tissue-specific settlement, but participates also in control of cell differentiation and proliferation and of T-cell effector functions. Blocking of CD11a function by specific antibodies or small molecules has become an important therapeutic approach in treatment of multiple inflammatory diseases. For example, humanized anti- CD11a antibody Efalizumab (Raptiva) is being used to interfere with T cell migration to sites of inflammation, binding of cholesterol-lowering drug simvastatin to CD11a allosteric site leads to immunomodulation and increase in lymphocytic cholinergic activity. Two transcript variants encoding different isoforms of CD11a have been found. Diseases associated with CD11a dysfunction include benign cephalic histiocytosis and leukocyte adhesion deficiency.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Product References

Modulating CD40 and integrin signaling in the proinflammatory nexus using a 15-amino-acid peptide, KGYY₁₅.

The Journal of biological chemistry

Vaitaitis GM,Wagner DH

Published figure using CD11a (LFA-1alpha) monoclonal antibody (Product # 11-0111-82) in Flow Cytometry

Mon May 01 00:00:00 EDT 2023

Adhesion Molecule Profile and the Effect of Anti-VLA-4 mAb Treatment in Experimental Autoimmune Encephalomyelitis, a Mouse Model of Multiple Sclerosis.

International journal of molecular sciences

Pyka-Fościak G,Lis GJ,Litwin JA

Published figure using CD11a (LFA-1alpha) monoclonal antibody (Product # 11-0111-82) in Immunohistochemistry

Fri Apr 22 00:00:00 EDT 2022

The arginine methyltransferase PRMT7 promotes extravasation of monocytes resulting in tissue injury in COPD.

Nature communications

Günes Günsel G,Conlon TM,Jeridi A,Kim R,Ertüz Z,Lang NJ,Ansari M,Novikova M,Jiang D,Strunz M,Gaianova M,Hollauer C,Gabriel C,Angelidis I,Doll S,Pestoni JC,Edelmann SL,Kohlhepp MS,Guillot A,Bassler K,Van Eeckhoutte HP,Kayalar Ö,Konyalilar N,Kanashova T,Rodius S,Ballester-López C,Genes Robles CM,Smirnova N,Rehberg M,Agarwal C,Krikki I,Piavaux B,Verleden SE,Vanaudenaerde B,Königshoff M,Dittmar G,Bracke KR,Schultze JL,Watz H,Eickelberg O,Stoeger T,Burgstaller G,Tacke F,Heissmeyer V,Rinkevich Y,Bayram H,Schiller HB,Conrad M,Schneider R,Yildirim AÖ

11-0111-82 was used in Flow Cytometry to show that in chronic obstructive pulmonary disease (COPD) patients, protein arginine methyltransferase 7 (PRMT7) expression is elevated in the lung tissue and localized to the macrophages.

Mon Mar 14 00:00:00 EDT 2022

Low immunogenicity of malaria pre-erythrocytic stages can be overcome by vaccination.

EMBO molecular medicine

Müller K,Gibbins MP,Roberts M,Reyes-Sandoval A,Hill AVS,Draper SJ,Matuschewski K,Silvie O,Hafalla JCR

11-0111-82 was used in Flow Cytometry to test the impact of pre-erythrocytic malaria immunogenicity on the immune system response.

Fri Apr 09 00:00:00 EDT 2021

Polymicrobial Sepsis Impairs Antigen-Specific Memory CD4 T Cell-Mediated Immunity.

Frontiers in immunology

Sjaastad FV,Kucaba TA,Dileepan T,Swanson W,Dail C,Cabrera-Perez J,Murphy KA,Badovinac VP,Griffith TS

11-0111-82 was used in Flow Cytometry to show CLP-induced sepsis alters the number and function of Ag-specific memory CD4 T cells, which contributes (in part) to the characteristic long-lasting immunoparalysis seen after sepsis.

Mon Apr 19 00:00:00 EDT 2021

CD4⁺ T Cell Fate Decisions Are Stochastic, Precede Cell Division, Depend on GITR Co-Stimulation, and Are Associated With Uropodium Development.

Frontiers in immunology

Cobbold SP,Adams E,Howie D,Waldmann H

11-0111-82 was used in Flow Cytometry to testing how manipulation of components of the microenvironment might be therapeutically exploited.

Mon Jan 31 00:00:00 EST 2022

Repeated Antigen Exposure Extends the Durability of Influenza-Specific Lung-Resident Memory CD8⁺ T Cells and Heterosubtypic Immunity.

Cell reports

Van Braeckel-Budimir N,Varga SM,Badovinac VP,Harty JT

11-0111 was used in Flow cytometry/Cell sorting to demonstrate that, relative to single virus exposure, repeated antigen exposure dramatically alters the dynamics of influenza-specific lung-resident primary memory CD8+ T cell populations.

Tue Sep 25 00:00:00 EDT 2018

Regulatory T Cell Migration Is Dependent on Glucokinase-Mediated Glycolysis.

Immunity

Kishore M,Cheung KCP,Fu H,Bonacina F,Wang G,Coe D,Ward EJ,Colamatteo A,Jangani M,Baragetti A,Matarese G,Smith DM,Haas R,Mauro C,Wraith DC,Okkenhaug K,Catapano AL,De Rosa V,Norata GD,Marelli-Berg FM

11-0111 was used in Flow cytometry/Cell sorting to demonstrate that glucokinase-dependent glycolysis regulates regulatory T cell migration.

Tue Nov 21 00:00:00 EST 2017

Trimethoprim-Sulfamethoxazole Prophylaxis During Live Malaria Sporozoite Immunization Induces Long-Lived, Homologous, and Heterologous Protective Immunity Against Sporozoite Challenge.

The Journal of infectious diseases

Hobbs CV,Anderson C,Neal J,Sahu T,Conteh S,Voza T,Langhorne J,Borkowsky W,Duffy PE

11-0111 was used in Flow cytometry/Cell sorting to demonstrate that repeated exposures to malaria parasites during TMP-SMX administration induces stage-specific and long-lived pre-erythrocytic protective anti-malarial immunity.

Sun Jan 01 00:00:00 EST 2017

From the Cover: Interplay Between IFN-γ and IL-6 Impacts the Inflammatory Response and Expression of Interferon-Regulated Genes in Environmental-Induced Autoimmunity.

Toxicological sciences : an official journal of the Society of Toxicology

Cauvi DM,Cauvi G,Toomey CB,Jacquinet E,Pollard KM

11-0111 was used in Flow cytometry/Cell sorting to study how the interplay between IFN-γ and IL-6 impacts the inflammatory response and expression of interferon-regulated genes in environmental-induced autoimmunity.

Sat Jul 01 00:00:00 EDT 2017

TGF-β receptor maintains CD4 T helper cell identity during chronic viral infections.

The Journal of clinical investigation

Lewis GM,Wehrens EJ,Labarta-Bajo L,Streeck H,Zuniga EI

11-0111 was used in Flow cytometry/Cell sorting to uncover an eomesodermin-driven CD4 T cell program that is continuously suppressed by TGF-β signalling.

Mon Oct 03 00:00:00 EDT 2016

Alterations in antigen-specific naive CD4 T cell precursors after sepsis impairs their responsiveness to pathogen challenge.

Journal of immunology (Baltimore, Md. : 1950)

Cabrera-Perez J,Condotta SA,James BR,Kashem SW,Brincks EL,Rai D,Kucaba TA,Badovinac VP,Griffith TS

11-0111 was used in Flow cytometry/Cell sorting to demonstrate that the recovery of CD4 T cells from sepsis-induced lymphopenia is accompanied by alterations to the composition and function of the Ag-specific CD4 T cell repertoire.

Sun Feb 15 00:00:00 EST 2015

Interleukin-7 and anti-programmed cell death 1 antibody have differing effects to reverse sepsis-induced immunosuppression.

Shock (Augusta, Ga.)

Shindo Y,Unsinger J,Burnham CA,Green JM,Hotchkiss RS

11-0111 was used in Flow cytometry/Cell sorting to investigate differences in immunomodulatory actions between IL-7 and anti-PD-1, to evaluate their potential use in the therapy of sepsis.

Wed Apr 01 00:00:00 EDT 2015

Anti-inflammatory functions of protein C require RAGE and ICAM-1 in a stimulus-dependent manner.

Mediators of inflammation

Braach N,Buschmann K,Pflaum J,Hudalla H,Koch L,Ryschich E,Poeschl J,Frommhold D

11-0111 was used in Flow cytometry/Cell sorting to show that RAGE and ICAM-1 are cooperatively involved in protein C-induced inhibition of leukocyte recruitment.

Sat Feb 07 00:00:00 EST 2015

Blockade of the negative co-stimulatory molecules PD-1 and CTLA-4 improves survival in primary and secondary fungal sepsis.

Critical care (London, England)

Chang KC,Burnham CA,Compton SM,Rasche DP,Mazuski RJ,McDonough JS,Unsinger J,Korman AJ,Green JM,Hotchkiss RS

11-0111 was used in Flow cytometry/Cell sorting to demonstrate the potential for therapy with anti-PD-1 in patients with life-threatening sepsis.

Sat May 11 00:00:00 EDT 2013

Pharmacological inhibition of p38 mitogen-activated protein kinases affects KC/CXCL1-induced intraluminal crawling, transendothelial migration, and chemotaxis of neutrophils in vivo.

Mediators of inflammation

Xu N,Hossain M,Liu L

11-0111 was used in Flow cytometry/Cell sorting to study the effect of a p38 MAPK inhibitor on Mac-1 expression in neutrophils after stimulation with chemokine KC.

Mon Aug 19 00:00:00 EDT 2013

Requirement of CD4 help for induction of CD8 T cell response specific for virally derived h60.

Immune network

Ryu SJ,Kang B,Kim SH,Kim TW,Chang J,Choi EY

11-0111 was used in Flow cytometry/Cell sorting to provide insights into the characterization of the CD8 T cell response specifically for antigens originating from cellular sources compared to viral sources.

Fri Jun 01 00:00:00 EDT 2012

A single-amino-acid variant of the H60 CD8 epitope generates specific immunity with diverse TCR recruitment.

Molecules and cells

Ryu SJ,Jeon JY,Chang J,Sproule TJ,Roopenian DC,Choi EY

11-0111 was used in Flow cytometry/Cell sorting to study the CD8 T-cell response to the the variant of the H60 minor histocompatibility antigen, H60H.

Sun Apr 01 00:00:00 EDT 2012

Rara haploinsufficiency modestly influences the phenotype of acute promyelocytic leukemia in mice.

Blood

Welch JS,Klco JM,Varghese N,Nagarajan R,Ley TJ

11-0111 was used in Flow cytometry/Cell sorting to test the prediction that RARA (retinoic acid receptor alpha) haploinsufficiency would contribute to acute promyelocytic leukemia (APL) pathogenesis.

Thu Feb 24 00:00:00 EST 2011

Characterization of CTL Clones Specific for Single Antigen, H60 Minor Histocompatibility Antigen.

Immune network

Jeon JY,Jung KM,Chang J,Choi EY

11-0111 was used in Flow cytometry/Cell sorting to show that Zbtb20 regulates astrocyte specification in the mouse neocortex.

Fri Apr 01 00:00:00 EDT 2011

β1 integrin is critical for the maintenance of antigen-specific CD4 T cells in the bone marrow but not long-term immunological memory.

Journal of immunology (Baltimore, Md. : 1950)

DeNucci CC,Shimizu Y

11-0111 was used in Flow cytometry/Cell sorting to demonstrate that the retention of CD4+ T cells in the bone marrow following a primary immune response is β1 integrin-dependent, but is not required for long-term CD4+ T cell memory.

Fri Apr 01 00:00:00 EDT 2011

Cutting edge: attrition of Plasmodium-specific memory CD8 T cells results in decreased protection that is rescued by booster immunization.

Journal of immunology (Baltimore, Md. : 1950)

Schmidt NW,Harty JT

11-0111 was used in Flow cytometry/Cell sorting to investigate whether unrelated infections will compromise numbers of Plasmodium-specific memory CD8 T cells, showing that attrition of Plasmodium-specific cells is rescued by booster immunisation.

Fri Apr 01 00:00:00 EDT 2011

Role for E-cadherin as an inhibitory receptor on epidermal gammadelta T cells.

Journal of immunology (Baltimore, Md. : 1950)

Uchida Y,Kawai K,Ibusuki A,Kanekura T

11-0111 was used in Flow cytometry/Cell sorting to analyse functional differences between E-cadherin and α(E)β(7) integrin expressed on murine dendritic epidermal T cells.

Wed Jun 15 00:00:00 EDT 2011

Vaginal protection and immunity after oral immunization of mice with a novel vaccine strain of Listeria monocytogenes expressing human immunodeficiency virus type 1 gag.

Journal of virology

Zhao X,Zhang M,Li Z,Frankel FR

11-0111 was used in Flow cytometry/Cell sorting to demonstrate that a Listeria monocytogenes-based vaccine expressing the HIV gag gene induces strong protective immune responses.

Fri Sep 01 00:00:00 EDT 2006

Pathogenicity and immunogenicity of a vaccine strain of Listeria monocytogenes that relies on a suicide plasmid to supply an essential gene product.

Infection and immunity

Zhao X,Li Z,Gu B,Frankel FR

11-0111 was used in Flow cytometry/Cell sorting to test the viability of an attractive vaccine candidate for the induction of protective cellular immune responses.

Thu Sep 01 00:00:00 EDT 2005

Bioinformatics

Protein Aliases: alpha L integrin; antigen CD11A p180; CD11 antigen-like family member A; CD11a; Integrin alpha-L; integrin alphaL; Leukocyte adhesion glycoprotein LFA-1 alpha chain; Leukocyte function-associated molecule 1 alpha chain; LFA-1A; Ly-15; Lymphocyte antigen 15; lymphocyte function associated antigen 1, alpha polypeptide

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Gene Aliases: (p180); Cd11a; Itgal; LFA-1; LFA-1A; Ly-15; Ly-21

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UniProt ID: (Mouse) P24063

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Entrez Gene ID: (Mouse) 16408

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Function(s)

ICAM-3 receptor activity protein complex binding metal ion binding protein heterodimerization activity cell adhesion molecule binding integrin

Process(es)

T cell activation via T cell receptor contact with antigen bound to MHC molecule on antigen presenting cell cell adhesion heterophilic cell-cell adhesion via plasma membrane cell adhesion molecules leukocyte cell-cell adhesion cell-matrix adhesion signal transduction cell surface receptor signaling pathway integrin-mediated signaling pathway single organismal cell-cell adhesion regulation of cell-cell adhesion positive regulation of cell-cell adhesion positive regulation of T cell proliferation receptor clustering activated T cell proliferation positive regulation of calcium-mediated signaling

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CD11a (LFA-1alpha) Monoclonal Antibody (M17/4), FITC, eBioscience™

CD11a (LFA-1alpha) Antibody (11-0111-82) in Flow

Product Details

11-0111-82

Immunohistochemistry (IHC)

Flow Cytometry (Flow)

Species Reactivity

Published species

Host/Isotype

Recommended Isotype Control

Class

Type

Clone

Conjugate

Excitation/Emission Max

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

How to use the Panel Builder

Bioinformatics

Performance Guarantee

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