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Invitrogen
Rat IgG2a kappa Isotype Control (eBR2a), FITC, eBioscience™
FIGURE: 1 / 5
Rat IgG2a kappa Isotype Control (11-4321-80) in Flow
Mouse bone marrow cells were surface stained with CD45R (B220) Monoclonal Antibody, eFluor 450 (Product # 48-0452-82). Cells were then stained intracellularly, using the Intracellular Fixation & Permeabilization Buffer Set (Product # 88-8824-00) and protocol, with 0.03 µg of Rat IgG2a kappa Isotype Control, FITC (Product # 11-4321-82) (left) or 0.03 µg of Galectin 9 Monoclonal Antibody, FITC (Product # 11-9... View More
Product Details
11-4321-80
Applications
Tested Dilution
Immunohistochemistry (IHC)
Assay-Dependent
Immunocytochemistry (ICC/IF)
Assay-Dependent
Flow Cytometry (Flow)
Assay-Dependent
Control (Ctrl)
Assay-Dependent
Product Specifications
Host/Isotype
Rat
/ IgG2a, kappa
Class
Monoclonal
Type
Isotype Control
Clone
eBR2a
Conjugate
Excitation/Emission Max
498/517 nm
View spectra
Form
Liquid
Concentration
0.5 mg/mL
Purification
Affinity chromatography
Storage buffer
PBS, pH 7.2
Contains
0.09% sodium azide
Storage conditions
4° C, store in dark, DO NOT FREEZE!
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_1834375
Product Specific Information
Description: The monoclonal rat IgG2a, kappa is useful as an isotype control immunoglobulin.
Applications Reported: This rat IgG2a isotype control has been reported for use in immunocytochemistry, immunohistochemistry, and flow cytometric analysis.
Applications Tested: This rat IgG2a isotype control has been tested by flow cytometric analysis of mouse splenocytes. This can be used at the same concentration as the experimental antibody.
Excitation: 488 nm; Emission: 520 nm; Laser: Blue Laser.
Filtration: 0.2 µm post-manufacturing filtered.
Target Information
Rat IgG2a is an immunoglobulin subclass that is commonly used in research and diagnostic applications. It is produced by the rat immune system and is known for its ability to activate complement and mediate antibody-dependent cell-mediated cytotoxicity (ADCC). Rat IgG2a has a molecular weight of approximately 160 kDa and is composed of two heavy chains and two light chains. It is often used as a control or reference antibody in experiments involving rat models, and is also commonly used in flow cytometry and immunohistochemistry applications.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: IgG2; Immunoglobulin G; Immunoglobulin G2; ImmunoglobulinG; ImmunoglobulinG2
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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