Invitrogen

CD16/CD32 Monoclonal Antibody (93), FITC, eBioscience™

CD16/CD32 Antibody in Flow Cytometry (Flow)

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CD16/CD32 Antibody (11-0161-82) in Flow

Staining of BALB/c splenocytes with Anti-Human/Mouse CD45R (B220) PE (Product # 12-0454-82) and 0.25 µg of Anti-Mouse CD16/CD32 FITC. Total viable cells were used for analysis.

CD16/CD32 Antibody in Immunocytochemistry, Flow Cytometry (ICC/IF, Flow)

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Product Details

11-0161-82

Applications

Tested Dilution

Publications

Immunocytochemistry (ICC/IF)

View 1 publication 1 publication

Flow Cytometry (Flow)

0.5 µg/test

View 20 publications 20 publications

Product Specifications

Species Reactivity

Mouse

Published species

Fruit fly, Human, Mouse

Host/Isotype

Rat / IgG2a, lambda

Recommended Isotype Control

Rat IgG2a kappa Isotype Control (eBR2a), FITC, eBioscience™

Class

Monoclonal

Type

Antibody

Clone

Conjugate

FITC FITC FITC

Excitation/Emission Max

498/517 nm View spectra

Form

Liquid

Concentration

0.5 mg/mL

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.2

Contains

0.09% sodium azide

Storage conditions

4° C, store in dark, DO NOT FREEZE!

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_464956

Product Specific Information

Description: The 93 monoclonal antibody reacts with an epitope shared by mouse CD16 and CD32. CD16 (Fc gamma III Receptor) and CD32 (Fc gamma II Receptor) are the low affinity receptors for the mouse IgG Fc portion and are expressed by B cells, monocyte/macrophages, NK cells, and neutrophils.

Applications Reported: The 93 antibody has been reported for use in flow cytometric analysis.

Applications Tested: The 93 antibody has been tested by flow cytometric analysis of mouse splenocytes. This can be used at less than or equal to 0.5 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

Excitation: 488 nm; Emission: 520 nm; Laser: Blue Laser.

Filtration: 0.2 µm post-manufacturing filtered.

Target Information

CD16 is a 50-65 kDa cell surface molecule that exists in two forms - a transmembranous form expressed by natural killer (NK) cells, and some T cells and a phosphatidylinositol linked form expressed by granulocytes. CD16 is a low affinity receptor for IgG (FcR III), and is an important receptor mediating ADCC (antibody dependent cell mediated cytotoxicity) by NK cells. IN comparison, CD32 is a 40 kD glycoprotein that acts as a low affinity receptor for IgG (also known as Fc gamma RII), and mediates several functions including endocytosis, activation of secretion, cytotoxicity and immunomodulation. CD32 is expressed by B cells, monocytes, granulocytes and platelets. Further, CD32 is involved in the phagocytosis of immune complexes, the regulation of antibody production by B-cells, and variations of the CD32 gene may increase vulnerability to systemic lupus erythematosus (SLE). Several transcript variants encoding different isoforms of CD32 have been found.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Product References

R274X-mutated Phf6 increased the self-renewal and skewed T cell differentiation of hematopoietic stem cells.

iScience

Lan Y,Yuan S,Guo T,Hou S,Zhao F,Yang W,Cao Y,Chu Y,Jiang E,Yuan W,Wang X

11-0161-82 was used in Flow cytometry/Cell sorting to demonstrate that Phf6R274X plays a critical role in fine-tuning T cells and HSC homeostasis.

Fri Jun 16 00:00:00 EDT 2023

Nonredundancy of IL-1α and IL-1β is defined by distinct regulation of tissues orchestrating resistance versus tolerance to infection.

Science advances

Eislmayr K,Bestehorn A,Morelli L,Borroni M,Vande Walle L,Lamkanfi M,Kovarik P

Published figure using CD16/CD32 monoclonal antibody (Product # 11-0161-82) in Flow Cytometry

Fri Mar 04 00:00:00 EST 2022

Aged hematopoietic stem cells are refractory to bloodborne systemic rejuvenation interventions.

The Journal of experimental medicine

Ho TT,Dellorusso PV,Verovskaya EV,Bakker ST,Flach J,Smith LK,Ventura PB,Lansinger OM,Hérault A,Zhang SY,Kang YA,Mitchell CA,Villeda SA,Passegué E

Published figure using CD16/CD32 monoclonal antibody (Product # 11-0161-82) in Flow Cytometry

Mon Jul 05 00:00:00 EDT 2021

C/EBPα and GATA-2 Mutations Induce Bilineage Acute Erythroid Leukemia through Transformation of a Neomorphic Neutrophil-Erythroid Progenitor.

Cancer cell

Di Genua C,Valletta S,Buono M,Stoilova B,Sweeney C,Rodriguez-Meira A,Grover A,Drissen R,Meng Y,Beveridge R,Aboukhalil Z,Karamitros D,Belderbos ME,Bystrykh L,Thongjuea S,Vyas P,Nerlov C

Published figure using CD16/CD32 monoclonal antibody (Product # 11-0161-82) in Flow Cytometry

Mon May 11 00:00:00 EDT 2020

Deregulated Notch and Wnt signaling activates early-stage myeloid regeneration pathways in leukemia.

The Journal of experimental medicine

Kang YA,Pietras EM,Passegué E

Published figure using CD16/CD32 monoclonal antibody (Product # 11-0161-82) in Flow Cytometry

Mon Mar 02 00:00:00 EST 2020

Ribonuclease T2 from Aspergillus fumigatus promotes T helper type 2 responses through M2 polarization of macrophages.

International journal of molecular medicine

Chen JJ,He YS,Zhong XJ,Cai ZL,Lyu YS,Zhao ZF,Ji K

11-0161 was used in Flow cytometry/Cell sorting to reveal that high rAf RNASET2 activity is required for rAf RNASET2‚induced M2 polarization of macrophages.

Sat Aug 01 00:00:00 EDT 2020

Progranulin prevents regulatory NK cell cytotoxicity against antiviral T cells.

JCI insight

Huang A,Shinde PV,Huang J,Senff T,Xu HC,Margotta C,Häussinger D,Willnow TE,Zhang J,Pandyra AA,Timm J,Weggen S,Lang KS,Lang PA

11-0161 was used in Flow cytometry/Cell sorting to show that progranulin (PGRN) can reduce NK cell cytotoxicity through reduction of NK cell expansion, granzyme B transcription, and NK cell-mediated lysis of target cells.

Thu Sep 05 00:00:00 EDT 2019

Murine CMV induces type 1 IFN that impairs differentiation of MDSCs critical for transplantation tolerance.

Blood advances

Dangi A,Zhang L,Zhang X,Luo X

11-0161 was used in Flow cytometry/Cell sorting to provide a solid foundation for seeking effective therapies for promoting transplantation tolerance in settings of CMV infection.

Tue Mar 27 00:00:00 EDT 2018

miR-143/145 differentially regulate hematopoietic stem and progenitor activity through suppression of canonical TGFβ signaling.

Nature communications

Lam J,van den Bosch M,Wegrzyn J,Parker J,Ibrahim R,Slowski K,Chang L,Martinez-Høyer S,Condorelli G,Boldin M,Deng Y,Umlandt P,Fuller M,Karsan A

Published figure using CD16/CD32 monoclonal antibody (Product # 11-0161-82) in Flow Cytometry

Wed Jun 20 00:00:00 EDT 2018

Myeloid progenitor cluster formation drives emergency and leukaemic myelopoiesis.

Nature

Hérault A,Binnewies M,Leong S,Calero-Nieto FJ,Zhang SY,Kang YA,Wang X,Pietras EM,Chu SH,Barry-Holson K,Armstrong S,Göttgens B,Passegué E

Published figure using CD16/CD32 monoclonal antibody (Product # 11-0161-82) in Flow Cytometry

Thu Apr 06 00:00:00 EDT 2017

Rational combination of oncolytic vaccinia virus and PD-L1 blockade works synergistically to enhance therapeutic efficacy.

Nature communications

Liu Z,Ravindranathan R,Kalinski P,Guo ZS,Bartlett DL

Published figure using CD16/CD32 monoclonal antibody (Product # 11-0161-82) in Flow Cytometry

Mon Mar 27 00:00:00 EDT 2017

lncRNA requirements for mouse acute myeloid leukemia and normal differentiation.

eLife

Delás MJ,Sabin LR,Dolzhenko E,Knott SR,Munera Maravilla E,Jackson BT,Wild SA,Kovacevic T,Stork EM,Zhou M,Erard N,Lee E,Kelley DR,Roth M,Barbosa IA,Zuber J,Rinn JL,Smith AD,Hannon GJ

11-0161 was used in Flow cytometry/Cell sorting to assess lncRNA function via an in vivo RNAi screen in a model of acute myeloid leukemia.

Wed Sep 06 00:00:00 EDT 2017

Gliotoxin Suppresses Macrophage Immune Function by Subverting Phosphatidylinositol 3,4,5-Trisphosphate Homeostasis.

mBio

Schlam D,Canton J,Carreño M,Kopinski H,Freeman SA,Grinstein S,Fairn GD

Published figure using CD16/CD32 monoclonal antibody (Product # 11-0161-82) in Flow Cytometry

Tue Apr 05 00:00:00 EDT 2016

Gliotoxin Suppresses Macrophage Immune Function by Subverting Phosphatidylinositol 3,4,5-Trisphosphate Homeostasis.

mBio

Schlam D,Canton J,Carreño M,Kopinski H,Freeman SA,Grinstein S,Fairn GD

Published figure using CD16/CD32 monoclonal antibody (Product # 11-0161-82) in Flow Cytometry

Tue Apr 05 00:00:00 EDT 2016

Agonist anti-GITR monoclonal antibody and stereotactic radiation induce immune-mediated survival advantage in murine intracranial glioma.

Journal for immunotherapy of cancer

Patel MA,Kim JE,Theodros D,Tam A,Velarde E,Kochel CM,Francica B,Nirschl TR,Ghasemzadeh A,Mathios D,Harris-Bookman S,Jackson CC,Jackson C,Ye X,Tran PT,Tyler B,Coric V,Selby M,Brem H,Drake CG,Pardoll DM,Lim M

Published figure using CD16/CD32 monoclonal antibody (Product # 11-0161-82) in Flow Cytometry

Wed May 18 00:00:00 EDT 2016

Role of caveolin-3 in lymphocyte activation.

Life sciences

Tran C,Stary CM,Schilling JM,Bentley B,Patel HH,Roth DM

11-0161 was used in Flow cytometry/Cell sorting to demonstrate that Cav-3 may be a novel participant in B-cell expression, T-cell cytokine production and activation of inflammation.

Thu Jan 15 00:00:00 EST 2015

Cytometric profiling in various clinical forms of multiple sclerosis with respect to CD21+, CD32+, and CD35+ B and T cells.

Translational neurodegeneration

Zandieh A,Izad M,Fakhri M,Amirifard H,Khazaeipour Z,Harirchian MH

11-0161 was used in Flow cytometry/Cell sorting to evaluate the frequency of various immune cell subsets expressing certain CD cell surface markers in MS clinical courses.

Tue Jul 02 00:00:00 EDT 2013

Unexpected role for MHC II-peptide complexes in shaping CD8 T-cell expansion and differentiation in vivo.

Proceedings of the National Academy of Sciences of the United States of America

Do JS,Valujskikh A,Vignali DA,Fairchild RL,Min B

11-0161 was used in Flow cytometry/Cell sorting to reveal a previously undefined MHC II-dependent regulation that tunes CD8 T-cell reactivity.

Tue Jul 31 00:00:00 EDT 2012

Morphine decreases bacterial phagocytosis by inhibiting actin polymerization through cAMP-, Rac-1-, and p38 MAPK-dependent mechanisms.

The American journal of pathology

Ninković J,Roy S

11-0161 was used in Flow cytometry/Cell sorting to study how long-term morphine treatment can lead to attenuation of actin polymerisation, FcgR-mediated phagocytosis, and decreased bacterial clearance.

Thu Mar 01 00:00:00 EST 2012

Morphine decreases bacterial phagocytosis by inhibiting actin polymerization through cAMP-, Rac-1-, and p38 MAPK-dependent mechanisms.

The American journal of pathology

Ninković J,Roy S

Thu Mar 01 00:00:00 EST 2012

In vivo inhibition of c-MYC in myeloid cells impairs tumor-associated macrophage maturation and pro-tumoral activities.

PloS one

Pello OM,Chèvre R,Laoui D,De Juan A,Lolo F,Andrés-Manzano MJ,Serrano M,Van Ginderachter JA,Andrés V

Published figure using CD16/CD32 monoclonal antibody (Product # 11-0161-82) in Flow Cytometry

Mon Feb 25 00:00:00 EST 2013

IL-3 is required for increases in blood basophils in nematode infection in mice and can enhance IgE-dependent IL-4 production by basophils in vitro.

Laboratory investigation; a journal of technical methods and pathology

Lantz CS,Min B,Tsai M,Chatterjea D,Dranoff G,Galli SJ

11-0161 was used in Flow cytometry/Cell sorting to implicate IL-3 as a major regulator of blood basophil level increases during Nippostrongylus brasiliensis and Strongyloides venezuelensis infection in mice.

Sat Nov 01 00:00:00 EDT 2008

Bioinformatics

Protein Aliases: CD16A; CD16B; CD32; CD32 receptor 2; Fc gamma receptor IIB; Fc gamma receptor III; Fc gamma RIIB; Fc-gamma RII; fc-gamma RIII; Fc-gamma-RII; fc-gamma-RIIB; Fcg receptor III; FCG2; FcgammaRIIB; FcgammaRIII; FCGR2C; FCGR3A; FCGR3B; FCGRIII; FCR-10; FcRII-b; FcRII-c; fcRIII; FCRIIIA; FGFR2B; IgG Fc receptor II beta; igG Fc receptor III; Low affinity immunoglobulin gamma Fc region receptor II; low affinity immunoglobulin gamma Fc region receptor II-b; low affinity immunoglobulin gamma Fc region receptor III; Ly-17; Lymphocyte antigen 17; RP11-5K23.1

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Gene Aliases: AI528646; CD16; CD32; F630109E10Rik; Fc[g]RII; Fcgr2; Fcgr2a; Fcgr2b; FcgRII; Fcr-2; Fcr-3; fcRII; Ly-17; Ly-m20; LyM-1

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UniProt ID: (Mouse) P08101

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Entrez Gene ID: (Mouse) 14130, (Mouse) 14131

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Function(s)

receptor signaling protein activity protein binding IgG binding immunoglobulin binding IgG receptor activity immunoglobulin receptor superfamily

Process(es)

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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CD16/CD32 Monoclonal Antibody (93), FITC, eBioscience™

CD16/CD32 Antibody (11-0161-82) in Flow

Product Details

11-0161-82

Immunocytochemistry (ICC/IF)

Flow Cytometry (Flow)

Species Reactivity

Published species

Host/Isotype

Recommended Isotype Control

Class

Type

Clone

Conjugate

Excitation/Emission Max

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

How to use the Panel Builder

Bioinformatics

Performance Guarantee

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