Invitrogen

CD38 Monoclonal Antibody (HIT2), FITC, eBioscience™

14 Published Figures

18 References

View all (171) CD38 antibodies

Datasheet

Protocols

Questions & Answers

Datasheet

Protocols

Questions & Answers

Antibody Testing Data (1)
Published Figures (14)

FIGURE: 1 / 15

CD38 Antibody (11-0389-42) in Flow

Staining of normal human peripheral blood cells with Mouse IgG1 K Isotype Control FITC (Product # 11-4714-42) (blue histogram) or Anti-Human CD38 FITC (purple histogram). Cells in the lymphocyte gate (left) and monocyte gate (right) were used for analysis.

Product Image Gallery

Product Details

11-0389-42

Applications

Tested Dilution

Publications

Flow Cytometry (Flow)

5 µL (1 µg)/test

View 17 publications 17 publications

ELISA (ELISA)

View 1 publication 1 publication

Product Specifications

Species Reactivity

Human

Published species

Human

Host/Isotype

Mouse / IgG1, kappa

Recommended Isotype Control

Mouse IgG1 kappa Isotype Control (P3.6.2.8.1), FITC, eBioscience™

Class

Monoclonal

Type

Antibody

Clone

Conjugate

FITC FITC FITC

Excitation/Emission Max

498/517 nm View spectra

Form

Liquid

Concentration

5 µL/Test

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.2, with 0.2% BSA

Contains

0.09% sodium azide

Storage conditions

4° C, store in dark, DO NOT FREEZE!

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_1724055

Product Specific Information

Description: The HIT2 monoclonal antibody reacts with the human CD38 molecule, an approximately 45 kDa type II transmembrane protein. CD38 is expressed by thymocytes, peripheral B cells including plasma cells, activated T cells, and monocytes. CD38 is a counter-receptor for CD31 and has both cyclase and hydrolase enzymatic activity.

Applications Reported: The HIT2 antibody has been reported for use in flow cytometric analysis.

Applications Tested: The HIT2 antibody has been pre-titrated and tested by flow cytometric analysis of normal human peripheral blood cells. This can be used at 5 µL (1 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test.

Excitation: 488 nm; Emission: 520 nm; Laser: Blue Laser.

Filtration: 0.2 µm post-manufacturing filtered.

Target Information

CD38 (NAD+ glycohydrolase) is a type II transmembrane glycoprotein able to induce activation, proliferation and differentiation of mature lymphocytes and mediate apoptosis of myeloid and lymphoid progenitor cells. CD38 functions as a multi-catalytic ectoenzyme serving as ADP-ribosyl cyclase, cyclic ADP-ribose hydrolase and possibly NAD+ glycohydrolase or as a cell surface receptor. Antibodies to CD38 are useful in subtyping of lymphomas and leukemias, detection of plasma cells (i.e. identification of myelomas), and as a marker for activated B and T cells. CD38 participates in cell adhesion, signal transduction and calcium signaling. Further, CD38 is expressed at high levels in the pancreas, liver, kidney, malignant lymphoma and neuroblastoma. Diseases associated with CD38 dysfunction include chronic lymphocytic leukemia and Richter's Syndrome.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

How to use the Panel Builder

Watch the video to learn how to use the Invitrogen Flow Cytometry Panel Builder to build your next flow cytometry panel in 5 easy steps.

Product References

Immunometabolic interference between cancer and COVID-19.

Frontiers in immunology

Consonni FM,Durante B,Manfredi M,Bleve A,Pandolfo C,Garlatti V,Vanella VV,Marengo E,Barberis E,Bottazzi B,Bombace S,My I,Condorelli G,Torri V,Sica A

Published figure using CD38 monoclonal antibody (Product # 11-0389-42) in Flow Cytometry

Tue Apr 18 00:00:00 EDT 2023

B cell-intrinsic changes with age do not impact antibody-secreting cell formation but delay B cell participation in the germinal centre reaction.

Aging cell

Lee JL,Fra-Bido SC,Burton AR,Innocentin S,Hill DL,Linterman MA

Published figure using CD38 monoclonal antibody (Product # 11-0389-42) in Flow Cytometry

Thu Sep 01 00:00:00 EDT 2022

Novel myeloma patient-derived xenograft models unveil the potency of anlotinib to overcome bortezomib resistance.

Frontiers in oncology

Yue Y,Cao Y,Mao X,Wang F,Fan P,Qian L,Guo S,Li F,Guo Y,Chen T,Lin Y,Dong W,Liu Y,Huang Y,Gu W

Published figure using CD38 monoclonal antibody (Product # 11-0389-42) in Flow Cytometry

Tue Aug 23 00:00:00 EDT 2022

Kaposi's sarcoma-associated herpesvirus latency-associated nuclear antigen dysregulates expression of MCL-1 by targeting FBW7.

PLoS pathogens

Kim YJ,Kim Y,Kumar A,Kim CW,Toth Z,Cho NH,Lee HR

Published figure using CD38 monoclonal antibody (Product # 11-0389-42) in Flow Cytometry

Fri Jan 01 00:00:00 EST 2021

Highly efficient CRISPR-Cas9-mediated gene knockout in primary human B cells for functional genetic studies of Epstein-Barr virus infection.

PLoS pathogens

Akidil E,Albanese M,Buschle A,Ruhle A,Pich D,Keppler OT,Hammerschmidt W

Published figure using CD38 monoclonal antibody (Product # 11-0389-42) in Flow Cytometry

Thu Apr 01 00:00:00 EDT 2021

SARS-CoV-2 infection paralyzes cytotoxic and metabolic functions of the immune cells.

Heliyon

Singh Y,Trautwein C,Fendel R,Krickeberg N,Berezhnoy G,Bissinger R,Ossowski S,Salker MS,Casadei N,Riess O

Published figure using CD38 monoclonal antibody (Product # 11-0389-42) in Flow Cytometry

Tue Jun 01 00:00:00 EDT 2021

Enhanced CXCR4 Expression of Human CD8^Low T Lymphocytes Is Driven by S1P₄.

Frontiers in immunology

Burkard T,Dreis C,Herrero San Juan M,Huhn M,Weigert A,Pfeilschifter JM,Radeke HH

Published figure using CD38 monoclonal antibody (Product # 11-0389-42) in Flow Cytometry

Wed Oct 20 00:00:00 EDT 2021

Synthesis of site-specific antibody-drug conjugates by ADP-ribosyl cyclases.

Science advances

Dai Z,Zhang XN,Nasertorabi F,Cheng Q,Li J,Katz BB,Smbatyan G,Pei H,Louie SG,Lenz HJ,Stevens RC,Zhang Y

Published figure using CD38 monoclonal antibody (Product # 11-0389-42) in ELISA

Mon Jun 01 00:00:00 EDT 2020

Chronic myelomonocytic leukaemia stem cell transcriptomes anticipate disease morphology and outcome.

EBioMedicine

Wiseman DH,Baker SM,Dongre AV,Gurashi K,Storer JA,Somervaille TC,Batta K

Published figure using CD38 monoclonal antibody (Product # 11-0389-42) in Flow Cytometry

Sat Aug 01 00:00:00 EDT 2020

IFNγ induces epigenetic programming of human T-bet^hi B cells and promotes TLR7/8 and IL-21 induced differentiation.

eLife

Zumaquero E,Stone SL,Scharer CD,Jenks SA,Nellore A,Mousseau B,Rosal-Vela A,Botta D,Bradley JE,Wojciechowski W,Ptacek T,Danila MI,Edberg JC,Bridges SL,Kimberly RP,Chatham WW,Schoeb TR,Rosenberg AF,Boss JM,Sanz I,Lund FE

Published figure using CD38 monoclonal antibody (Product # 11-0389-42) in Flow Cytometry

Wed May 15 00:00:00 EDT 2019

Abnormal phenotypic features of IgM+B cell subsets in patients with chronic hepatitis C virus infection.

Experimental and therapeutic medicine

Kong F,Feng B,Zhang H,Rao H,Wang J,Cong X,Wei L

Published figure using CD38 monoclonal antibody (Product # 11-0389-42) in Flow Cytometry

Tue Aug 01 00:00:00 EDT 2017

Heterogeneous leukemia stem cells in myeloid blast phase chronic myeloid leukemia.

Blood advances

Kinstrie R,Karamitros D,Goardon N,Morrison H,Hamblin M,Robinson L,Clark RE,Copland M,Vyas P

11-0389 was used in Flow cytometry/Cell sorting to study the haemopoietic hierarchy of additional mutations in blast phase chronic myeloid leukaemia, and the cellular identity of the resulting leukaemia-propagating stem cell.

Tue Dec 27 00:00:00 EST 2016

The upregulation of LAG-3 on T cells defines a subpopulation with functional exhaustion and correlates with disease progression in HIV-infected subjects.

Journal of immunology (Baltimore, Md. : 1950)

Tian X,Zhang A,Qiu C,Wang W,Yang Y,Qiu C,Liu A,Zhu L,Yuan S,Hu H,Wang W,Wei Q,Zhang X,Xu J

11-0389 was used in Flow cytometry/Cell sorting to study the role of LAG-3 on T cell functionality during persistent HIV-1 infection.

Wed Apr 15 00:00:00 EDT 2015

Generation of functional, antigen-specific CD8+ human T cells from cord blood stem cells using exogenous Notch and tetramer-TCR signaling.

Stem cells (Dayton, Ohio)

Fernandez I,Ooi TP,Roy K

Published figure using CD38 monoclonal antibody (Product # 11-0389-42) in Flow Cytometry

Wed Jan 01 00:00:00 EST 2014

Generation of functional, antigen-specific CD8+ human T cells from cord blood stem cells using exogenous Notch and tetramer-TCR signaling.

Stem cells (Dayton, Ohio)

Fernandez I,Ooi TP,Roy K

Published figure using CD38 monoclonal antibody (Product # 11-0389-42) in Flow Cytometry

Wed Jan 01 00:00:00 EST 2014

CD4⁺CD73⁺ T cells are associated with lower T-cell activation and C reactive protein levels and are depleted in HIV-1 infection regardless of viral suppression.

AIDS (London, England)

Schuler PJ,Macatangay BJ,Saze Z,Jackson EK,Riddler SA,Buchanan WG,Hilldorfer BB,Mellors JW,Whiteside TL,Rinaldo CR

11-0389 was used in Flow cytometry/Cell sorting to assess suppression of T-cell function by exogenous ADO and CD4+ CD73+ T cells.

Wed Jun 19 00:00:00 EDT 2013

Incomplete normalization of regulatory t-cell frequency in the gut mucosa of Colombian HIV-infected patients receiving long-term antiretroviral treatment.

PloS one

Rueda CM,Velilla PA,Chougnet CA,Rugeles MT

11-0389 was used in Flow cytometry/Cell sorting to assess the frequency and phenotype of regulatory T-cells within CMV and HIV patients on different treatment regimes.

Tue Sep 23 00:00:00 EDT 2014

Targeting CDK1 promotes FLT3-activated acute myeloid leukemia differentiation through C/EBPα.

The Journal of clinical investigation

Radomska HS,Alberich-Jordà M,Will B,Gonzalez D,Delwel R,Tenen DG

Published figure using CD38 monoclonal antibody (Product # 11-0389-42) in Flow Cytometry

Wed Aug 01 00:00:00 EDT 2012

Generation of bivalent chromatin domains during cell fate decisions.

Epigenetics & chromatin

De Gobbi M,Garrick D,Lynch M,Vernimmen D,Hughes JR,Goardon N,Luc S,Lower KM,Sloane-Stanley JA,Pina C,Soneji S,Renella R,Enver T,Taylor S,Jacobsen SE,Vyas P,Gibbons RJ,Higgs DR

11-0389 was used in Flow cytometry/Cell sorting to investigate the patterns and levels of histone modifications at bivalent genes, showing the generation of bivalent chromatin domains during cell fate decisions.

Mon Jun 06 00:00:00 EDT 2011

Bioinformatics

Protein Aliases: 2'-phospho-ADP-ribosyl cyclase; 2'-phospho-ADP-ribosyl cyclase/2'-phospho-cyclic-ADP-ribose transferase; 2'-phospho-cyclic-ADP-ribose transferase; ADP-ribosyl cyclase 1; ADP-ribosyl cyclase/cyclic ADP-ribose hydrolase 1; ADPRC 1; cADPR hydrolase 1; CD38; CD38 antigen (p45); CD38 antigen p45; cluster of differentiation 38; Cyclic ADP-ribose hydrolase 1; ecto-nicotinamide adenine dinucleotide glycohydrolase; NAD(+) nucleosidase; NAD+ nucleosidase; T10

View more View less

Gene Aliases: ADPRC 1; ADPRC1; CD38

View more View less

UniProt ID: (Human) P28907

View more View less

Entrez Gene ID: (Human) 952

View more View less

Function(s)

NAD+ nucleosidase activity transferase activity phosphorus-oxygen lyase activity NAD(P)+ nucleosidase activity cyclase lyase metabolite interconversion enzyme

Process(es)

response to hypoxia signal transduction positive regulation of cytosolic calcium ion concentration female pregnancy positive regulation of cell growth positive regulation of B cell proliferation positive regulation of insulin secretion response to estradiol response to retinoic acid response to progesterone response to hydroperoxide response to drug negative regulation of apoptotic process negative regulation of bone resorption negative regulation of transcription, DNA-templated positive regulation of transcription, DNA-templated positive regulation of vasoconstriction B cell receptor signaling pathway long term synaptic depression response to interleukin-1 apoptotic signaling pathway

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

Learn more

CD38 Monoclonal Antibody (HIT2), FITC, eBioscience™

CD38 Antibody (11-0389-42) in Flow

Product Details

11-0389-42

Flow Cytometry (Flow)

ELISA (ELISA)

Species Reactivity

Published species

Host/Isotype

Recommended Isotype Control

Class

Type

Clone

Conjugate

Excitation/Emission Max

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

How to use the Panel Builder

Bioinformatics

Performance Guarantee

We're here to help

Your items have has been added!