Invitrogen

CD38 Monoclonal Antibody (HIT2), APC, eBioscience™

15 Published Figures

29 References

View all (171) CD38 antibodies

Datasheet

Protocols

Questions & Answers

Datasheet

Protocols

Questions & Answers

Antibody Testing Data (1)
Published Figures (15)

FIGURE: 1 / 16

CD38 Antibody (17-0389-42) in Flow

Staining of normal human peripheral blood cells with Mouse IgG1 K Isotype Control APC (Product # 17-4714-81) (blue histogram) or Anti-Human CD38 APC (purple histogram). Cells in the lymphocyte gate (left) and monocyte gate (right) were used for analysis.

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Product Details

17-0389-42

Applications

Tested Dilution

Publications

Flow Cytometry (Flow)

5 µL (0.25 µg)/test

View 29 publications 29 publications

Product Specifications

Species Reactivity

Human

Published species

Human

Host/Isotype

Mouse / IgG1, kappa

Recommended Isotype Control

Mouse IgG1 kappa Isotype Control (P3.6.2.8.1), APC, eBioscience™

Class

Monoclonal

Type

Antibody

Clone

Conjugate

APC APC APC

Excitation/Emission Max

651/660 nm View spectra

Form

Liquid

Concentration

5 µL/Test

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.2, with 0.2% BSA

Contains

0.09% sodium azide

Storage conditions

4° C, store in dark, DO NOT FREEZE!

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_1834353

Product Specific Information

Description: The HIT2 monoclonal antibody reacts with the human CD38 molecule, an approximately 45 kDa type II transmembrane protein. CD38 is expressed by thymocytes, peripheral B cells including plasma cells, activated T cells, and monocytes. CD38 is a counter-receptor for CD31 and has both cyclase and hydrolase enzymatic activity.

Applications Reported: This HIT2 antibody has been reported for use in flow cytometric analysis.

Applications Tested: This HIT2 antibody has been pre-titrated and tested by flow cytometric analysis of normal human peripheral blood cells. This can be used at 5 µL (0.25 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test.

Excitation: 633-647 nm; Emission: 660 nm; Laser: Red Laser.

Filtration: 0.2 µm post-manufacturing filtered.

Target Information

CD38 (NAD+ glycohydrolase) is a type II transmembrane glycoprotein able to induce activation, proliferation and differentiation of mature lymphocytes and mediate apoptosis of myeloid and lymphoid progenitor cells. CD38 functions as a multi-catalytic ectoenzyme serving as ADP-ribosyl cyclase, cyclic ADP-ribose hydrolase and possibly NAD+ glycohydrolase or as a cell surface receptor. Antibodies to CD38 are useful in subtyping of lymphomas and leukemias, detection of plasma cells (i.e. identification of myelomas), and as a marker for activated B and T cells. CD38 participates in cell adhesion, signal transduction and calcium signaling. Further, CD38 is expressed at high levels in the pancreas, liver, kidney, malignant lymphoma and neuroblastoma. Diseases associated with CD38 dysfunction include chronic lymphocytic leukemia and Richter's Syndrome.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Product References

Immunometabolic interference between cancer and COVID-19.

Frontiers in immunology

Consonni FM,Durante B,Manfredi M,Bleve A,Pandolfo C,Garlatti V,Vanella VV,Marengo E,Barberis E,Bottazzi B,Bombace S,My I,Condorelli G,Torri V,Sica A

Published figure using CD38 monoclonal antibody (Product # 17-0389-42) in Flow Cytometry

Tue Apr 18 00:00:00 EDT 2023

B cell-intrinsic changes with age do not impact antibody-secreting cell formation but delay B cell participation in the germinal centre reaction.

Aging cell

Lee JL,Fra-Bido SC,Burton AR,Innocentin S,Hill DL,Linterman MA

17-0389-42 was used in Flow cytometry/Cell sorting to show that B cells from aged individuals are not intrinsically defective in responding to stimulation and becoming antibody-secreting cells, implicating B cell-extrinsic factors as the primary cause of age-associated impairment in the humoral immunity.

Thu Sep 01 00:00:00 EDT 2022

B cell-intrinsic changes with age do not impact antibody-secreting cell formation but delay B cell participation in the germinal centre reaction.

Aging cell

Lee JL,Fra-Bido SC,Burton AR,Innocentin S,Hill DL,Linterman MA

Thu Sep 01 00:00:00 EDT 2022

Novel myeloma patient-derived xenograft models unveil the potency of anlotinib to overcome bortezomib resistance.

Frontiers in oncology

Yue Y,Cao Y,Mao X,Wang F,Fan P,Qian L,Guo S,Li F,Guo Y,Chen T,Lin Y,Dong W,Liu Y,Huang Y,Gu W

Published figure using CD38 monoclonal antibody (Product # 17-0389-42) in Flow Cytometry

Tue Aug 23 00:00:00 EDT 2022

scTAM-seq enables targeted high-confidence analysis of DNA methylation in single cells.

Genome biology

Bianchi A,Scherer M,Zaurin R,Quililan K,Velten L,Beekman R

17-0389-42 was used in Flow cytometry/Cell sorting to demonstrate that scTAM-seq can resolve DNA methylation dynamics across B-cell differentiation in blood and bone marrow, identifying intermediate differentiation states that were previously masked.

Fri Oct 28 00:00:00 EDT 2022

Kaposi's sarcoma-associated herpesvirus latency-associated nuclear antigen dysregulates expression of MCL-1 by targeting FBW7.

PLoS pathogens

Kim YJ,Kim Y,Kumar A,Kim CW,Toth Z,Cho NH,Lee HR

Published figure using CD38 monoclonal antibody (Product # 17-0389-42) in Flow Cytometry

Fri Jan 01 00:00:00 EST 2021

Highly efficient CRISPR-Cas9-mediated gene knockout in primary human B cells for functional genetic studies of Epstein-Barr virus infection.

PLoS pathogens

Akidil E,Albanese M,Buschle A,Ruhle A,Pich D,Keppler OT,Hammerschmidt W

Published figure using CD38 monoclonal antibody (Product # 17-0389-42) in Flow Cytometry

Thu Apr 01 00:00:00 EDT 2021

SARS-CoV-2 infection paralyzes cytotoxic and metabolic functions of the immune cells.

Heliyon

Singh Y,Trautwein C,Fendel R,Krickeberg N,Berezhnoy G,Bissinger R,Ossowski S,Salker MS,Casadei N,Riess O

Published figure using CD38 monoclonal antibody (Product # 17-0389-42) in Flow Cytometry

Tue Jun 01 00:00:00 EDT 2021

Enhanced CXCR4 Expression of Human CD8^Low T Lymphocytes Is Driven by S1P₄.

Frontiers in immunology

Burkard T,Dreis C,Herrero San Juan M,Huhn M,Weigert A,Pfeilschifter JM,Radeke HH

17-0389-42 was used in Flow Cytometry to investigate the influence of IL-33 and S1P receptor 4 (S1P4) on the differentiation and migration of human CD8+ T lymphocytes.

Wed Oct 20 00:00:00 EDT 2021

Enhanced CXCR4 Expression of Human CD8^Low T Lymphocytes Is Driven by S1P₄.

Frontiers in immunology

Burkard T,Dreis C,Herrero San Juan M,Huhn M,Weigert A,Pfeilschifter JM,Radeke HH

17-0389-42 was used in Flow Cytometry to investigate the influence of IL-33 and S1P receptor 4 (S1P4) on the differentiation and migration of human CD8+ T lymphocytes.

Wed Oct 20 00:00:00 EDT 2021

Transitions in lineage specification and gene regulatory networks in hematopoietic stem/progenitor cells over human development.

Cell reports

Roy A,Wang G,Iskander D,O'Byrne S,Elliott N,O'Sullivan J,Buck G,Heuston EF,Wen WX,Meira AR,Hua P,Karadimitris A,Mead AJ,Bodine DM,Roberts I,Psaila B,Thongjuea S

17-0389-42 was used in Flow cytometry/Cell sorting to demonstrate the utility of this dataset for understanding aberrant hematopoiesis through comparison to two cancers that present at distinct time points in postnatal life-juvenile myelomonocytic leukemia, a childhood cancer, and myelofibrosis, which classically presents in older adults.

Tue Sep 14 00:00:00 EDT 2021

Genetic manipulation and immortalized culture of ex vivo primary human germinal center B cells.

Nature protocols

Caeser R,Gao J,Di Re M,Gong C,Hodson DJ

17-0389-42 was used in Flow Cytometry to develop a methodology for the purification and ex vivo expansion of primary, nonmalignant human germinal center B cells.

Sat May 01 00:00:00 EDT 2021

Chronic myelomonocytic leukaemia stem cell transcriptomes anticipate disease morphology and outcome.

EBioMedicine

Wiseman DH,Baker SM,Dongre AV,Gurashi K,Storer JA,Somervaille TC,Batta K

Published figure using CD38 monoclonal antibody (Product # 17-0389-42) in Flow Cytometry

Sat Aug 01 00:00:00 EDT 2020

Patient iPSC-Derived Macrophages to Study Inborn Errors of the IFN-γ Responsive Pathway.

Cells

Haake K,Neehus AL,Buchegger T,Kühnel MP,Blank P,Philipp F,Oleaga-Quintas C,Schulz A,Grimley M,Goethe R,Jonigk D,Kalinke U,Boisson-Dupuis S,Casanova JL,Bustamante J,Lachmann N

17-0389 was used in Flow cytometry/Cell sorting to report a disease modeling platform to study the role of macrophages in patients with inborn errors of IFN-γ immunity.

Wed Feb 19 00:00:00 EST 2020

IFNγ induces epigenetic programming of human T-bet^hi B cells and promotes TLR7/8 and IL-21 induced differentiation.

eLife

Zumaquero E,Stone SL,Scharer CD,Jenks SA,Nellore A,Mousseau B,Rosal-Vela A,Botta D,Bradley JE,Wojciechowski W,Ptacek T,Danila MI,Edberg JC,Bridges SL,Kimberly RP,Chatham WW,Schoeb TR,Rosenberg AF,Boss JM,Sanz I,Lund FE

Published figure using CD38 monoclonal antibody (Product # 17-0389-42) in Flow Cytometry

Wed May 15 00:00:00 EDT 2019

A transcriptionally and functionally distinct PD-1⁺ CD8⁺ T cell pool with predictive potential in non-small-cell lung cancer treated with PD-1 blockade.

Nature medicine

Thommen DS,Koelzer VH,Herzig P,Roller A,Trefny M,Dimeloe S,Kiialainen A,Hanhart J,Schill C,Hess C,Savic Prince S,Wiese M,Lardinois D,Ho PC,Klein C,Karanikas V,Mertz KD,Schumacher TN,Zippelius A

Sun Jul 01 00:00:00 EDT 2018

Vitamin D supplementation during pregnancy: Effect on the neonatal immune system in a randomized controlled trial.

The Journal of allergy and clinical immunology

Hornsby E,Pfeffer PE,Laranjo N,Cruikshank W,Tuzova M,Litonjua AA,Weiss ST,Carey VJ,O'Connor G,Hawrylowicz C

17-0389 was used in Flow cytometry/Cell sorting to investigate the effect on neonatal immunity of maternal supplementation with 4400 IU/d vitamin D3 during the second and third trimesters of pregnancy by using a subset of cord blood samples from a randomized, double-blind, placebo-controlled clinical trial.

Mon Jan 01 00:00:00 EST 2018

CD16⁺NK-92 and anti-CD123 monoclonal antibody prolongs survival in primary human acute myeloid leukemia xenografted mice.

Haematologica

Williams BA,Wang XH,Leyton JV,Maghera S,Deif B,Reilly RM,Minden MD,Keating A

17-0389 was used in Flow cytometry/Cell sorting to study whether CD16+ NK-92 cell line combined with an antibody targeting a leukaemic stem cell antigen can lead to improved survival in a relevant pre-clinical model of acute myeloid leukaemia.

Mon Oct 01 00:00:00 EDT 2018

Genetic Architecture of Adaptive Immune System Identifies Key Immune Regulators.

Cell reports

Lagou V,Garcia-Perez JE,Smets I,Van Horebeek L,Vandebergh M,Chen L,Mallants K,Prezzemolo T,Hilven K,Humblet-Baron S,Moisse M,Van Damme P,Boeckxstaens G,Bowness P,Dubois B,Dooley J,Liston A,Goris A

17-0389-42 was used in Flow Cytometry to identify eight genome-wide significant associations explaining 6%-20% of variance in immune system regulation.

Tue Oct 16 00:00:00 EDT 2018

Abnormal phenotypic features of IgM+B cell subsets in patients with chronic hepatitis C virus infection.

Experimental and therapeutic medicine

Kong F,Feng B,Zhang H,Rao H,Wang J,Cong X,Wei L

Tue Aug 01 00:00:00 EDT 2017

Effects of acute exposure to low-dose radiation on the characteristics of human bone marrow mesenchymal stromal/stem cells.

Inflammation and regeneration

Fujishiro A,Miura Y,Iwasa M,Fujii S,Sugino N,Andoh A,Hirai H,Maekawa T,Ichinohe T

17-0389 was used in Flow cytometry/Cell sorting to determine whether low dose irradiation affects the function of mesenchymal stromal/stem cells.

Fri Mar 11 00:00:00 EST 2022

PD-L1 mediated the differentiation of tumor-infiltrating CD19⁺ B lymphocytes and T cells in Invasive breast cancer.

Oncoimmunology

Guan H,Lan Y,Wan Y,Wang Q,Wang C,Xu L,Chen Y,Liu W,Zhang X,Li Y,Gu Y,Wang Z,Xie F

17-0389 was used in Flow cytometry/Cell sorting to investigate the role of B cells in human breast cancer etiology.

Mon Feb 01 00:00:00 EST 2016

Renal Transplant Recipients Treated with Calcineurin-Inhibitors Lack Circulating Immature Transitional CD19+CD24hiCD38hi Regulatory B-Lymphocytes.

PloS one

Tebbe B,Wilde B,Ye Z,Wang J,Wang X,Jian F,Dolff S,Schedlowski M,Hoyer PF,Kribben A,Witzke O,Hoerning A

17-0389 was used in Flow cytometry/Cell sorting to analyse the effect of calcineurin inhibition on peripherally circulating regulatory B cells in renal transplant recipients receiving cyclosporine A or tacrolimus.

Mon Aug 29 00:00:00 EDT 2016

SF3B1 mutant MDS-initiating cells may arise from the haematopoietic stem cell compartment.

Nature communications

Mian SA,Rouault-Pierre K,Smith AE,Seidl T,Pizzitola I,Kizilors A,Kulasekararaj AG,Bonnet D,Mufti GJ

17-0389 was used in Flow cytometry/Cell sorting to demonstrate that mutations to SF3B1 can arise from CD34(+)CD38(-)CD45RA(-)CD90(+)CD49f(+) haematopoietic stem cells and is an initiating event in myelodysplastic syndrome patients.

Tue Dec 08 00:00:00 EST 2015

Immunologic human renal allograft injury associates with an altered IL-10/TNF-α expression ratio in regulatory B cells.

Journal of the American Society of Nephrology : JASN

Cherukuri A,Rothstein DM,Clark B,Carter CR,Davison A,Hernandez-Fuentes M,Hewitt E,Salama AD,Baker RJ

17-0389 was used in Flow cytometry/Cell sorting to explore proinflammatory cytokine expression in B-cell subsets.

Tue Jul 01 00:00:00 EDT 2014

Generation of functional, antigen-specific CD8+ human T cells from cord blood stem cells using exogenous Notch and tetramer-TCR signaling.

Stem cells (Dayton, Ohio)

Fernandez I,Ooi TP,Roy K

Published figure using CD38 monoclonal antibody (Product # 17-0389-42) in Flow Cytometry

Wed Jan 01 00:00:00 EST 2014

Generation of functional, antigen-specific CD8+ human T cells from cord blood stem cells using exogenous Notch and tetramer-TCR signaling.

Stem cells (Dayton, Ohio)

Fernandez I,Ooi TP,Roy K

Published figure using CD38 monoclonal antibody (Product # 17-0389-42) in Flow Cytometry

Wed Jan 01 00:00:00 EST 2014

DNA methylation profiling in human B cells reveals immune regulatory elements and epigenetic plasticity at Alu elements during B-cell activation.

Genome research

Lai AY,Mav D,Shah R,Grimm SA,Phadke D,Hatzi K,Melnick A,Geigerman C,Sobol SE,Jaye DL,Wade PA

17-0389 was used in Flow cytometry/Cell sorting to investigate the profile of B-lymphocyte subsets during different stages of the humoral immune response in humans.

Sun Dec 01 00:00:00 EST 2013

Targeting CDK1 promotes FLT3-activated acute myeloid leukemia differentiation through C/EBPα.

The Journal of clinical investigation

Radomska HS,Alberich-Jordà M,Will B,Gonzalez D,Delwel R,Tenen DG

Published figure using CD38 monoclonal antibody (Product # 17-0389-42) in Flow Cytometry

Wed Aug 01 00:00:00 EDT 2012

Human tonsil B-cell lymphoma 6 (BCL6)-expressing CD4+ T-cell subset specialized for B-cell help outside germinal centers.

Proceedings of the National Academy of Sciences of the United States of America

Bentebibel SE,Schmitt N,Banchereau J,Ueno H

17-0389 was used in Flow cytometry/Cell sorting to identify and investigate the role of a previously undefined BCL6-expressing CD4(+) T-cell subset in human tonsils.

Tue Aug 16 00:00:00 EDT 2011

Further phenotypic characterization of the primitive lineage- CD34+CD38-CD90+CD45RA- hematopoietic stem cell/progenitor cell sub-population isolated from cord blood, mobilized peripheral blood and patients with chronic myelogenous leukemia.

Blood cancer journal

Wisniewski D,Affer M,Willshire J,Clarkson B

17-0389 was used in Flow cytometry/Cell sorting to investigate the purification of haematopoietic stem cells, and the potential targetable differences between primitive normal and chronic myelogenous leukaemia stem/progenitor cells.

Thu Sep 01 00:00:00 EDT 2011

Fc receptor-like 3 protein expressed on IL-2 nonresponsive subset of human regulatory T cells.

Journal of immunology (Baltimore, Md. : 1950)

Nagata S,Ise T,Pastan I

17-0389 was used in Flow cytometry/Cell sorting to study the effects of Foxp3 and IL-2 on FCRL3-/+ nTreg cell subsets.

Mon Jun 15 00:00:00 EDT 2009

Bioinformatics

Protein Aliases: 2'-phospho-ADP-ribosyl cyclase; 2'-phospho-ADP-ribosyl cyclase/2'-phospho-cyclic-ADP-ribose transferase; 2'-phospho-cyclic-ADP-ribose transferase; ADP-ribosyl cyclase 1; ADP-ribosyl cyclase/cyclic ADP-ribose hydrolase 1; ADPRC 1; cADPR hydrolase 1; CD38; CD38 antigen (p45); CD38 antigen p45; cluster of differentiation 38; Cyclic ADP-ribose hydrolase 1; ecto-nicotinamide adenine dinucleotide glycohydrolase; NAD(+) nucleosidase; NAD+ nucleosidase; T10

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Gene Aliases: ADPRC 1; ADPRC1; CD38

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UniProt ID: (Human) P28907

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Entrez Gene ID: (Human) 952

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Function(s)

NAD+ nucleosidase activity transferase activity phosphorus-oxygen lyase activity NAD(P)+ nucleosidase activity cyclase lyase metabolite interconversion enzyme

Process(es)

response to hypoxia signal transduction positive regulation of cytosolic calcium ion concentration female pregnancy positive regulation of cell growth positive regulation of B cell proliferation positive regulation of insulin secretion response to estradiol response to retinoic acid response to progesterone response to hydroperoxide response to drug negative regulation of apoptotic process negative regulation of bone resorption negative regulation of transcription, DNA-templated positive regulation of transcription, DNA-templated positive regulation of vasoconstriction B cell receptor signaling pathway long term synaptic depression response to interleukin-1 apoptotic signaling pathway

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CD38 Monoclonal Antibody (HIT2), APC, eBioscience™

CD38 Antibody (17-0389-42) in Flow

Product Details

17-0389-42

Flow Cytometry (Flow)

Species Reactivity

Published species

Host/Isotype

Recommended Isotype Control

Class

Type

Clone

Conjugate

Excitation/Emission Max

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

How to use the Panel Builder

Bioinformatics

Performance Guarantee

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