Invitrogen

CD49d (Integrin alpha 4) Monoclonal Antibody (R1-2), FITC, eBioscience™

11 Published Figures

19 References

View all (56) CD49d (Integrin alpha 4) antibodies

Datasheet

Protocols

Questions & Answers

Datasheet

Protocols

Questions & Answers

Antibody Testing Data (1)
Published Figures (11)

CD49d (Integrin alpha 4) Antibody in Flow Cytometry (Flow)

FIGURE: 1 / 12

CD49d (Integrin alpha 4) Antibody (11-0492-82) in Flow

Staining of BALB/c splenocytes with 0.25 µg of Rat IgG2b K Isotype Control FITC (Product # 11-4031-82) (blue histogram) or 0.25 µg of Anti-Mouse CD49d (Integrin alpha 4) FITC (purple histogram). Cells in the lymphocyte gate were used for analysis.

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Product Details

11-0492-82

Applications

Tested Dilution

Publications

Immunocytochemistry (ICC/IF)

View 1 publication 1 publication

Flow Cytometry (Flow)

0.5 µg/test

View 18 publications 18 publications

Product Specifications

Species Reactivity

Mouse

Published species

Mouse

Host/Isotype

Rat / IgG2b, kappa

Recommended Isotype Control

Rat IgG2b kappa Isotype Control (eB149/10H5), FITC, eBioscience™

Class

Monoclonal

Type

Antibody

Clone

R1-2

Conjugate

FITC FITC FITC

Excitation/Emission Max

498/517 nm View spectra

Form

Liquid

Concentration

0.5 mg/mL

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.2

Contains

0.09% sodium azide

Storage conditions

4° C, store in dark, DO NOT FREEZE!

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_465083

Product Specific Information

Description: The R1-2 monoclonal antibody reacts with mouse CD49d, the 150 kDa integrin alpha4 subunit. The complex of CD49d non-covalently associated with integrin beta1 (CD29), also known as VLA-4, is a receptor for fibronectin and VCAM-1 (CD106). This complex is expressed by thymocytes, peripheral lymphocytes, monocytes and eosinophils. CD49d also associates with integrin b7 and binds to the Mucosal vascular Addressin (MAdCAM-1).

Applications Reported: The R1-2 antibody has been reported for use in flow cytometric analysis.

Applications Tested: The R1-2 antibody has been tested by flow cytometric analysis of mouse splenocytes. This can be used at less than or equal to 0.5 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

Excitation: 488 nm; Emission: 520 nm; Laser: Blue Laser.

Filtration: 0.2 µm post-manufacturing filtered.

Target Information

The integrin alpha 4 (also known as CD49d and ITGA4) belongs to the integrin alpha chain family of proteins. Integrins are heterodimeric integral membrane proteins composed of an alpha and beta chains. Alpha4 chain associates with either beta1 or beta7 chain. It has been demonstrated that the putative ligand-binding sites of both integrin alpha4beta1 and alpha4beta7 is located on the alpha4 chain. These ligands included Madcam, VCAM, and fibronectin. Madcam is known as the principal ligand for integrin alpha4beta7. Recently it was also demonstrated that HIV-1 envelope can mimic Madcam by binding to and signaling though integrin alpha4beta7, the gut mucosal homing receptor for peripheral T cells.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Product References

Maternal inappropriate calcium intake aggravates dietary-induced obesity in male offspring by affecting the differentiation potential of mesenchymal stem cells.

World journal of stem cells

Li P,Wang Y,Li P,Liu YL,Liu WJ,Chen XY,Tang TT,Qi KM,Zhang Y

11-0492-82 was used in Flow cytometry/Cell sorting to show that early-life abnormal dietary calcium intake might program the adipogenic differentiation potential of BMSCs from male offspring, with significant expressions on the Wnt/β-catenin signaling pathway to aggravate high-fat-diet-induced obesity in adulthood.

Wed Oct 26 00:00:00 EDT 2022

Low immunogenicity of malaria pre-erythrocytic stages can be overcome by vaccination.

EMBO molecular medicine

Müller K,Gibbins MP,Roberts M,Reyes-Sandoval A,Hill AVS,Draper SJ,Matuschewski K,Silvie O,Hafalla JCR

Published figure using CD49d (Integrin alpha 4) monoclonal antibody (Product # 11-0492-82) in Flow Cytometry

Fri Apr 09 00:00:00 EDT 2021

Nidogen-1 Contributes to the Interaction Network Involved in Pro-B Cell Retention in the Peri-sinusoidal Hematopoietic Stem Cell Niche.

Cell reports

Balzano M,De Grandis M,Vu Manh TP,Chasson L,Bardin F,Farina A,Sergé A,Bidaut G,Charbord P,Hérault L,Bailly AL,Cartier-Michaud A,Boned A,Dalod M,Duprez E,Genever P,Coles M,Bajenoff M,Xerri L,Aurrand-Lions M,Schiff C,Mancini SJC

11-0492 was used in Flow cytometry/Cell sorting to study pro-B cells in their support of stroll cells and their interaction network allowing their retention.

Tue Mar 19 00:00:00 EDT 2019

A Macrophage Colony-Stimulating-Factor-Producing γδ T Cell Subset Prevents Malarial Parasitemic Recurrence.

Immunity

Mamedov MR,Scholzen A,Nair RV,Cumnock K,Kenkel JA,Oliveira JHM,Trujillo DL,Saligrama N,Zhang Y,Rubelt F,Schneider DS,Chien YH,Sauerwein RW,Davis MM

Published figure using CD49d (Integrin alpha 4) monoclonal antibody (Product # 11-0492-82) in Flow Cytometry

Tue Feb 20 00:00:00 EST 2018

Interleukin-18 Amplifies Macrophage Polarization and Morphological Alteration, Leading to Excessive Angiogenesis.

Frontiers in immunology

Kobori T,Hamasaki S,Kitaura A,Yamazaki Y,Nishinaka T,Niwa A,Nakao S,Wake H,Mori S,Yoshino T,Nishibori M,Takahashi H

Published figure using CD49d (Integrin alpha 4) monoclonal antibody (Product # 11-0492-82) in Flow Cytometry

Wed Mar 27 00:00:00 EDT 2019

Helminth-induced IL-4 expands bystander memory CD8⁺ T cells for early control of viral infection.

Nature communications

Rolot M,Dougall AM,Chetty A,Javaux J,Chen T,Xiao X,Machiels B,Selkirk ME,Maizels RM,Hokke C,Denis O,Brombacher F,Vanderplasschen A,Gillet L,Horsnell WGC,Dewals BG

Published figure using CD49d (Integrin alpha 4) monoclonal antibody (Product # 11-0492-82) in Flow Cytometry

Tue Oct 30 00:00:00 EDT 2018

Differentiation and Protective Capacity of Virus-Specific CD8⁺ T Cells Suggest Murine Norovirus Persistence in an Immune-Privileged Enteric Niche.

Immunity

Tomov VT,Palko O,Lau CW,Pattekar A,Sun Y,Tacheva R,Bengsch B,Manne S,Cosma GL,Eisenlohr LC,Nice TJ,Virgin HW,Wherry EJ

11-0492 was used in Flow cytometry/Cell sorting to elucidate a strategy of immune evasion by mouse norovirus via the induction of a CD8+ T cell program normally reserved for latent pathogens and persistence in an immune-privileged enteric niche.

Tue Oct 17 00:00:00 EDT 2017

A Multistage Subunit Vaccine Effectively Protects Mice Against Primary Progressive Tuberculosis, Latency and Reactivation.

EBioMedicine

Ma J,Teng X,Wang X,Fan X,Wu Y,Tian M,Zhou Z,Li L

11-0492-82 was used in Flow cytometry/Cell sorting to conclude that CMFO-DMT represents a promising candidate to prevent the occurrence of adult TB through both prophylactic and therapeutic methods, and warrants assessment in preclinical and clinical trials.

Tue Aug 01 00:00:00 EDT 2017

Resolving TYK2 locus genotype-to-phenotype differences in autoimmunity.

Science translational medicine

Dendrou CA,Cortes A,Shipman L,Evans HG,Attfield KE,Jostins L,Barber T,Kaur G,Kuttikkatte SB,Leach OA,Desel C,Faergeman SL,Cheeseman J,Neville MJ,Sawcer S,Compston A,Johnson AR,Everett C,Bell JI,Karpe F,Ultsch M,Eigenbrot C,McVean G,Fugger L

11-0492 was used in Flow cytometry/Cell sorting to reveal a protective homozygous effect that defined a signalling optimum between autoimmunity and immunodeficiency and identified TYK2 as a potential drug target for autoimmune disorders.

Wed Nov 02 00:00:00 EDT 2016

The impact of ranitidine on monocyte responses in the context of solid tumors.

Oncotarget

Vila-Leahey A,Rogers D,Marshall JS

Published figure using CD49d (Integrin alpha 4) monoclonal antibody (Product # 11-0492-82) in Flow Cytometry

Tue Mar 08 00:00:00 EST 2016

Interleukin-7 and anti-programmed cell death 1 antibody have differing effects to reverse sepsis-induced immunosuppression.

Shock (Augusta, Ga.)

Shindo Y,Unsinger J,Burnham CA,Green JM,Hotchkiss RS

Published figure using CD49d (Integrin alpha 4) monoclonal antibody (Product # 11-0492-82) in Flow Cytometry

Wed Apr 01 00:00:00 EDT 2015

Type I interferons regulate eomesodermin expression and the development of unconventional memory CD8(+) T cells.

Nature communications

Martinet V,Tonon S,Torres D,Azouz A,Nguyen M,Kohler A,Flamand V,Mao CA,Klein WH,Leo O,Goriely S

11-0492 was used in Immunohistochemistry to demonstrate that type I interferon signalling regulates the function and homeostasis of memory-like CD8(+) T cells.

Fri May 08 00:00:00 EDT 2015

Leishmania amazonensis infection impairs dendritic cell migration from the inflammatory site to the draining lymph node.

BMC infectious diseases

Hermida MD,Doria PG,Taguchi AM,Mengel JO,dos-Santos W

11-0492 was used in Flow cytometry/Cell sorting to study the effect of Leishmania amazonensis infection on the ability of inflammatory phagocyte populations to migrate from an inflammatory site to the draining lymph node.

Wed Aug 20 00:00:00 EDT 2014

Persistent enteric murine norovirus infection is associated with functionally suboptimal virus-specific CD8 T cell responses.

Journal of virology

Tomov VT,Osborne LC,Dolfi DV,Sonnenberg GF,Monticelli LA,Mansfield K,Virgin HW,Artis D,Wherry EJ

11-0492 was used in Flow cytometry/Cell sorting to investigate the magnitude, location, and dynamics of the murine norovirus-specific immune response.

Sat Jun 01 00:00:00 EDT 2013

B cell activating factor inhibition impairs bacterial immunity by reducing T cell-independent IgM secretion.

Infection and immunity

Jones DD,Jones M,DeIulio GA,Racine R,MacNamara KC,Winslow GM

11-0492 was used in Flow cytometry/Cell sorting to determine that BAFF signalling can directly regulate secretory processes.

Sun Dec 01 00:00:00 EST 2013

Heterologous immunity triggered by a single, latent virus in Mus musculus: combined costimulation- and adhesion- blockade decrease rejection.

PloS one

Beus JM,Hashmi SS,Selvaraj SA,Duan D,Stempora LL,Monday SA,Cheeseman JA,Hamby KM,Speck SH,Larsen CP,Kirk AD,Kean LS

11-0492 was used in Flow cytometry/Cell sorting to study changes in CD8(dim) T cell populations in response to a MHV68 rodent model of latent virus-induced heterologous alloimmunity.

Mon Apr 14 00:00:00 EDT 2014

IgM production by bone marrow plasmablasts contributes to long-term protection against intracellular bacterial infection.

Journal of immunology (Baltimore, Md. : 1950)

Racine R,McLaughlin M,Jones DD,Wittmer ST,MacNamara KC,Woodland DL,Winslow GM

11-0492 was used in Flow cytometry/Cell sorting to identify a cell population responsible for IgM production in the bone marrow that is maintained in the absence of chronic infection.

Sat Jan 15 00:00:00 EST 2011

Chemerin contributes to inflammation by promoting macrophage adhesion to VCAM-1 and fibronectin through clustering of VLA-4 and VLA-5.

Journal of immunology (Baltimore, Md. : 1950)

Hart R,Greaves DR

11-0492 was used in Immunofluorescence-cell culture cells to suggest a novel role for chemerin in the recruitment and retention of macrophages at sites of inflammation.

Wed Sep 15 00:00:00 EDT 2010

Plasmacytoid dendritic cells employ multiple cell adhesion molecules sequentially to interact with high endothelial venule cells--molecular basis of their trafficking to lymph nodes.

International immunology

Matsutani T,Tanaka T,Tohya K,Otani K,Jang MH,Umemoto E,Taniguchi K,Hayasaka H,Ueda K,Miyasaka M

11-0492 was used in Flow cytometry/Cell sorting to investigate the molecular mechanisms of plasmacytoid dendritic cell migration using freshly isolated dendritic cells and high endothelial venule cells.

Sat Sep 01 00:00:00 EDT 2007

Bioinformatics

Protein Aliases: CD49 antigen-like family member D; CD49d; Integrin alpha-4; Integrin alpha-IV; integrin alpha4B; ITGA-IV; LPAM subunit alpha; Lymphocyte Peyer patch adhesion molecules subunit alpha; VLA-4 receptor, alpha 4 subunit; VLA-4 subunit alpha

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Gene Aliases: CD49D; Itga4; Itga4B

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UniProt ID: (Mouse) Q00651

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Entrez Gene ID: (Mouse) 16401

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Function(s)

fibronectin binding metal ion binding cell adhesion molecule binding protein antigen binding integrin

Process(es)

blood vessel remodeling positive regulation of leukocyte migration cell-matrix adhesion involved in ameboidal cell migration cell adhesion heterophilic cell-cell adhesion via plasma membrane cell adhesion molecules leukocyte cell-cell adhesion cell-matrix adhesion integrin-mediated signaling pathway heart development single organismal cell-cell adhesion cell migration heterotypic cell-cell adhesion substrate adhesion-dependent cell spreading receptor clustering leukocyte tethering or rolling diapedesis face development mesenchyme development chorio-allantoic fusion visceral serous pericardium development T cell migration negative regulation of protein homodimerization activity import into cell positive regulation of leukocyte cell-cell adhesion positive regulation of leukocyte tethering or rolling positive regulation of T cell migration

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If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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CD49d (Integrin alpha 4) Monoclonal Antibody (R1-2), FITC, eBioscience™

CD49d (Integrin alpha 4) Antibody (11-0492-82) in Flow

Product Details

11-0492-82

Immunocytochemistry (ICC/IF)

Flow Cytometry (Flow)

Species Reactivity

Published species

Host/Isotype

Recommended Isotype Control

Class

Type

Clone

Conjugate

Excitation/Emission Max

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

How to use the Panel Builder

Bioinformatics

Performance Guarantee

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