Invitrogen

CD49d (Integrin alpha 4) Monoclonal Antibody (R1-2), PE, eBioscience™

11 Published Figures

18 References

View all (56) CD49d (Integrin alpha 4) antibodies

Datasheet

Protocols

Questions & Answers

Datasheet

Protocols

Questions & Answers

Antibody Testing Data (1)
Published Figures (11)

CD49d (Integrin alpha 4) Antibody in Flow Cytometry (Flow)

FIGURE: 1 / 12

CD49d (Integrin alpha 4) Antibody (12-0492-82) in Flow

Staining of BALB/c splenocytes with 0.5 µg of Rat IgG2b K Isotype Control PE (Product # 12-4031-82) (blue histogram) or 0.5 µg of Anti-Mouse CD49d (Integrin alpha 4) PE (purple histogram). Cells in the lymphocyte gate were used for analysis.

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Product Details

12-0492-82

Applications

Tested Dilution

Publications

Flow Cytometry (Flow)

1 µg/test

View 18 publications 18 publications

Product Specifications

Species Reactivity

Mouse

Published species

Human, Mouse

Host/Isotype

Rat / IgG2b, kappa

Recommended Isotype Control

Rat IgG2b kappa Isotype Control (eB149/10H5), PE, eBioscience™

Class

Monoclonal

Type

Antibody

Clone

R1-2

Conjugate

PE PE PE

Excitation/Emission Max

565/576 nm View spectra

Form

Liquid

Concentration

0.2 mg/mL

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.2

Contains

0.09% sodium azide

Storage conditions

4° C, store in dark, DO NOT FREEZE!

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_465697

Product Specific Information

Description: The R1-2 monoclonal antibody reacts with mouse CD49d, the 150 kDa integrin alpha4 subunit. The complex of CD49d non-covalently associated with integrin beta1 (CD29), also known as VLA-4, is a receptor for fibronectin and VCAM-1 (CD106). This complex is expressed by thymocytes, peripheral lymphocytes, monocytes and eosinophils. CD49d also associates with integrin b7 and binds to the Mucosal vascular Addressin (MAdCAM-1).

Applications Reported: The R1-2 antibody has been reported for use in flow cytometric analysis.

Applications Tested: The R1-2 antibody has been tested by flow cytometric analysis of mouse splenocytes. This can be used at less than or equal to 1 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

Excitation: 488-561 nm; Emission: 578 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser.

Filtration: 0.2 µm post-manufacturing filtered.

Target Information

The integrin alpha 4 (also known as CD49d and ITGA4) belongs to the integrin alpha chain family of proteins. Integrins are heterodimeric integral membrane proteins composed of an alpha and beta chains. Alpha4 chain associates with either beta1 or beta7 chain. It has been demonstrated that the putative ligand-binding sites of both integrin alpha4beta1 and alpha4beta7 is located on the alpha4 chain. These ligands included Madcam, VCAM, and fibronectin. Madcam is known as the principal ligand for integrin alpha4beta7. Recently it was also demonstrated that HIV-1 envelope can mimic Madcam by binding to and signaling though integrin alpha4beta7, the gut mucosal homing receptor for peripheral T cells.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Product References

Low immunogenicity of malaria pre-erythrocytic stages can be overcome by vaccination.

EMBO molecular medicine

Müller K,Gibbins MP,Roberts M,Reyes-Sandoval A,Hill AVS,Draper SJ,Matuschewski K,Silvie O,Hafalla JCR

12-0492-82 was used in Flow Cytometry to test the impact of pre-erythrocytic malaria immunogenicity on the immune system response.

Fri Apr 09 00:00:00 EDT 2021

Low immunogenicity of malaria pre-erythrocytic stages can be overcome by vaccination.

EMBO molecular medicine

Müller K,Gibbins MP,Roberts M,Reyes-Sandoval A,Hill AVS,Draper SJ,Matuschewski K,Silvie O,Hafalla JCR

12-0492-82 was used in Flow Cytometry to test the impact of pre-erythrocytic malaria immunogenicity on the immune system response.

Fri Apr 09 00:00:00 EDT 2021

Diverse CD8 T Cell Responses to Viral Infection Revealed by the Collaborative Cross.

Cell reports

Martin MD,Sompallae R,Winborn CS,Harty JT,Badovinac VP

12-0492 was used in Flow cytometry/Cell sorting to advance the utility of the Collaborative Cross (CC) as a tool to analyze the immune response to viral infection.

Tue Apr 14 00:00:00 EDT 2020

A Macrophage Colony-Stimulating-Factor-Producing γδ T Cell Subset Prevents Malarial Parasitemic Recurrence.

Immunity

Mamedov MR,Scholzen A,Nair RV,Cumnock K,Kenkel JA,Oliveira JHM,Trujillo DL,Saligrama N,Zhang Y,Rubelt F,Schneider DS,Chien YH,Sauerwein RW,Davis MM

Published figure using CD49d (Integrin alpha 4) monoclonal antibody (Product # 12-0492-82) in Flow Cytometry

Tue Feb 20 00:00:00 EST 2018

Interleukin-18 Amplifies Macrophage Polarization and Morphological Alteration, Leading to Excessive Angiogenesis.

Frontiers in immunology

Kobori T,Hamasaki S,Kitaura A,Yamazaki Y,Nishinaka T,Niwa A,Nakao S,Wake H,Mori S,Yoshino T,Nishibori M,Takahashi H

Published figure using CD49d (Integrin alpha 4) monoclonal antibody (Product # 12-0492-82) in Flow Cytometry

Wed Mar 27 00:00:00 EDT 2019

Helminth-induced IL-4 expands bystander memory CD8⁺ T cells for early control of viral infection.

Nature communications

Rolot M,Dougall AM,Chetty A,Javaux J,Chen T,Xiao X,Machiels B,Selkirk ME,Maizels RM,Hokke C,Denis O,Brombacher F,Vanderplasschen A,Gillet L,Horsnell WGC,Dewals BG

Published figure using CD49d (Integrin alpha 4) monoclonal antibody (Product # 12-0492-82) in Flow Cytometry

Tue Oct 30 00:00:00 EDT 2018

Common helical V1V2 conformations of HIV-1 Envelope expose the α4β7 binding site on intact virions.

Nature communications

Wibmer CK,Richardson SI,Yolitz J,Cicala C,Arthos J,Moore PL,Morris L

12-0492 was used in Flow cytometry/Cell sorting to elucidate the cocrystal structures of two α4Β7-blocking antibodies from an infected donor complexed with scaffolded gp120 V1V2 or V2 peptides.

Fri Oct 26 00:00:00 EDT 2018

Lactate dehydrogenase activity drives hair follicle stem cell activation.

Nature cell biology

Flores A,Schell J,Krall AS,Jelinek D,Miranda M,Grigorian M,Braas D,White AC,Zhou JL,Graham NA,Graeber T,Seth P,Evseenko D,Coller HA,Rutter J,Christofk HR,Lowry WE

12-0492-82 was used in Flow cytometry/Cell sorting to suggest that HFSCs maintain a metabolic state that allows them to remain dormant and yet quickly respond to appropriate proliferative stimuli.

Fri Sep 01 00:00:00 EDT 2017

The impact of ranitidine on monocyte responses in the context of solid tumors.

Oncotarget

Vila-Leahey A,Rogers D,Marshall JS

12-0492 was used in Flow cytometry/Cell sorting to examine the impact of oral ranitidine treatment on multiple murine tumour models.

Tue Mar 08 00:00:00 EST 2016

Laminin 521 maintains differentiation potential of mouse and human satellite cell-derived myoblasts during long-term culture expansion.

Skeletal muscle

Penton CM,Badarinarayana V,Prisco J,Powers E,Pincus M,Allen RE,August PR

12-0492 was used in Flow cytometry/Cell sorting to demonstrate laminin 521 is a superior substrate for both short-term and long-term myogenic cell culture applications compared to other commonly utilized substrates.

Tue Dec 13 00:00:00 EST 2016

Interleukin-7 and anti-programmed cell death 1 antibody have differing effects to reverse sepsis-induced immunosuppression.

Shock (Augusta, Ga.)

Shindo Y,Unsinger J,Burnham CA,Green JM,Hotchkiss RS

12-0492 was used in Flow cytometry/Cell sorting to investigate differences in immunomodulatory actions between IL-7 and anti-PD-1, to evaluate their potential use in the therapy of sepsis.

Wed Apr 01 00:00:00 EDT 2015

Two separate defects affecting true naive or virtual memory T cell precursors combine to reduce naive T cell responses with aging.

Journal of immunology (Baltimore, Md. : 1950)

Renkema KR,Li G,Wu A,Smithey MJ,Nikolich-Žugich J

12-0492 was used in Flow cytometry/Cell sorting to study the causes and components of the age-related erosion of naive T cell responses.

Wed Jan 01 00:00:00 EST 2014

Leishmania amazonensis infection impairs dendritic cell migration from the inflammatory site to the draining lymph node.

BMC infectious diseases

Hermida MD,Doria PG,Taguchi AM,Mengel JO,dos-Santos W

12-0492 was used in Flow cytometry/Cell sorting to study the effect of Leishmania amazonensis infection on the ability of inflammatory phagocyte populations to migrate from an inflammatory site to the draining lymph node.

Wed Aug 20 00:00:00 EDT 2014

PI3-kinase γ promotes Rap1a-mediated activation of myeloid cell integrin α4β1, leading to tumor inflammation and growth.

PloS one

Schmid MC,Franco I,Kang SW,Hirsch E,Quilliam LA,Varner JA

12-0492 was used in Flow cytometry/Cell sorting to suggest novel avenues for cancer therapy by manipulating the PI3Kγ-Rap1a-dependent pathway.

Wed Oct 30 00:00:00 EDT 2013

Plasmacytoid dendritic cells transport peripheral antigens to the thymus to promote central tolerance.

Immunity

Hadeiba H,Lahl K,Edalati A,Oderup C,Habtezion A,Pachynski R,Nguyen L,Ghodsi A,Adler S,Butcher EC

12-0492 was used in Flow cytometry/Cell sorting to explore the mechanisms underlying the contribution to immune tolerance conducted by peripheral dendritic cells.

Fri Mar 23 00:00:00 EDT 2012

Molecular imaging of very late antigen-4 (α4β1 integrin) in the premetastatic niche.

Journal of nuclear medicine : official publication, Society of Nuclear Medicine

Shokeen M,Zheleznyak A,Wilson JM,Jiang M,Liu R,Ferdani R,Lam KS,Schwarz JK,Anderson CJ

12-0492 was used in Flow cytometry/Cell sorting to study bone marrow-derived cellular expression of VEGFR1 and VLA-4 in the development of a premetastatic niche.

Tue May 01 00:00:00 EDT 2012

Role for E-cadherin as an inhibitory receptor on epidermal gammadelta T cells.

Journal of immunology (Baltimore, Md. : 1950)

Uchida Y,Kawai K,Ibusuki A,Kanekura T

12-0492 was used in Flow cytometry/Cell sorting to analyse functional differences between E-cadherin and α(E)β(7) integrin expressed on murine dendritic epidermal T cells.

Wed Jun 15 00:00:00 EDT 2011

Control of alpha4beta7 integrin expression and CD4 T cell homing by the beta1 integrin subunit.

Journal of immunology (Baltimore, Md. : 1950)

DeNucci CC,Pagán AJ,Mitchell JS,Shimizu Y

12-0492 was used in Flow cytometry/Cell sorting to investigate the role of the beta 1 subunit of integrin on CD4 T cell homing.

Mon Mar 01 00:00:00 EST 2010

A homing mechanism for bone marrow-derived progenitor cell recruitment to the neovasculature.

The Journal of clinical investigation

Jin H,Aiyer A,Su J,Borgstrom P,Stupack D,Friedlander M,Varner J

12-0492-82 was used in Flow Cytometry to show that integrin alpha4beta1 promotes the homing of circulating progenitor cells to the alpha4beta1 ligands VCAM and cellular fibronectin which are expressed on actively remodelling neovasculature.

Wed Mar 01 00:00:00 EST 2006

Bioinformatics

Protein Aliases: CD49 antigen-like family member D; CD49d; Integrin alpha-4; Integrin alpha-IV; integrin alpha4B; ITGA-IV; LPAM subunit alpha; Lymphocyte Peyer patch adhesion molecules subunit alpha; VLA-4 receptor, alpha 4 subunit; VLA-4 subunit alpha

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Gene Aliases: CD49D; Itga4; Itga4B

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UniProt ID: (Mouse) Q00651

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Entrez Gene ID: (Mouse) 16401

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Function(s)

fibronectin binding metal ion binding cell adhesion molecule binding protein antigen binding integrin

Process(es)

blood vessel remodeling positive regulation of leukocyte migration cell-matrix adhesion involved in ameboidal cell migration cell adhesion heterophilic cell-cell adhesion via plasma membrane cell adhesion molecules leukocyte cell-cell adhesion cell-matrix adhesion integrin-mediated signaling pathway heart development single organismal cell-cell adhesion cell migration heterotypic cell-cell adhesion substrate adhesion-dependent cell spreading receptor clustering leukocyte tethering or rolling diapedesis face development mesenchyme development chorio-allantoic fusion visceral serous pericardium development T cell migration negative regulation of protein homodimerization activity import into cell positive regulation of leukocyte cell-cell adhesion positive regulation of leukocyte tethering or rolling positive regulation of T cell migration

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CD49d (Integrin alpha 4) Monoclonal Antibody (R1-2), PE, eBioscience™

CD49d (Integrin alpha 4) Antibody (12-0492-82) in Flow

Product Details

12-0492-82

Flow Cytometry (Flow)

Species Reactivity

Published species

Host/Isotype

Recommended Isotype Control

Class

Type

Clone

Conjugate

Excitation/Emission Max

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

How to use the Panel Builder

Bioinformatics

Performance Guarantee

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