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Invitrogen
Rat IgG2b kappa Isotype Control (eB149/10H5), PE, eBioscience™
FIGURE: 1 / 3
Rat IgG2b kappa Isotype Control (12-4031-82) in Flow
Mouse bone marrow cells were stained with CD11b Monoclonal Antibody, PE-Cyanine7 (Product # 25-0112-82) and 0.25 µg of Rat IgG2b kappa Isotype Control, PE (Product # 12-4031-83) (left) or 0.25 µg of CD88 (C5aR1) Monoclonal Antibody, PE (right). Total viable cells were used for analysis, as determined by Fixable Viability Dye eFluor 450 (Product # 65-0863-18).
Product Details
12-4031-82
Applications
Tested Dilution
Flow Cytometry (Flow)
Assay-Dependent
Control (Ctrl)
Assay-Dependent
Product Specifications
Host/Isotype
Rat
/ IgG2b, kappa
Class
Monoclonal
Type
Isotype Control
Clone
eB149/10H5
Conjugate
Excitation/Emission Max
565/576 nm
View spectra
Form
Liquid
Concentration
0.2 mg/mL
Purification
Affinity chromatography
Storage buffer
PBS, pH 7.2
Contains
0.09% sodium azide
Storage conditions
4° C, store in dark, DO NOT FREEZE!
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_470042
Product Specific Information
Description: The rat IgG2b, kappa monoclonal antibody is useful as an isotype control immunoglobulin.
Applications Reported: This monoclonal rat IgG2b kappa isotype control has been reported for use in flow cytometric analysis.
Applications Tested: Rat IgG2b Isotype Control has been tested by flow cytometric analysis of mouse splenocyte suspensions. It should be used at the same concentration as the experimental antibody.
Excitation: 488-561 nm; Emission: 578 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser.
Filtration: 0.2 µm post-manufacturing filtered.
Target Information
Rat IgG2b is a protein-coding gene that encodes for the immunoglobulin G2b (IgG2b) subclass of rat antibodies. IgG2b is a major subclass of rat immunoglobulins that plays a crucial role in the immune response by neutralizing pathogens and promoting opsonization. Rat IgG2b is commonly used in research applications, such as in immunohistochemistry, flow cytometry, and Western blotting.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: IgG2; Immunoglobulin G; Immunoglobulin G2; ImmunoglobulinG; ImmunoglobulinG2
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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