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Invitrogen
Rat IgG2b kappa Isotype Control (eB149/10H5), Alexa Fluor™ 660, eBioscience™
FIGURE: 1 / 1
Rat IgG2b kappa Isotype Control (606-4031-81) in Flow
BALB/c mouse splenocytes were stained with TER-119 Monoclonal Antibody, eFluor 450 (Product # 48-5921-82) and staining buffer (autofluorescence) (left) or 0.125 µg of Rat IgG2b kappa Isotype Control, Alexa Fluor 660 (right). Cells in the lymphocyte gate were used for analysis. This data was collected on a 5-laser Cytek Aurora full spectral cytometer.
Product Details
606-4031-81
Applications
Tested Dilution
Flow Cytometry (Flow)
Assay-Dependent
Control (Ctrl)
Assay-Dependent
Product Specifications
Host/Isotype
Rat
/ IgG2b, kappa
Class
Monoclonal
Type
Isotype Control
Clone
eB149/10H5
Conjugate
Excitation/Emission Max
663/691 nm
View spectra
Form
Liquid
Concentration
0.2 mg/mL
Purification
Affinity chromatography
Storage buffer
PBS, pH 7.2
Contains
0.09% sodium azide
Storage conditions
4° C, store in dark, DO NOT FREEZE!
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2896270
Product Specific Information
Description: The rat IgG2b kappa monoclonal antibody is useful as an isotype control immunoglobulin.
Applications Reported: This eB149/10H5 antibody has been reported for use in flow cytometric analysis.
Applications Tested: This eB149/10H5 antibody has been tested by flow cytometric analysis of mouse splenocytes and normal human peripheral blood cells and should be used at the same concentration as the experimental antibody.
Alexa Fluor 660 emits at 690 nm and is intended for use on spectral cytometers where it may be multiplexed with APC, Alexa Fluor 647 and Alexa Fluor 700.
Excitation: 662 nm; Emission: 690 nm; Laser: Red Laser
Target Information
Rat IgG2b is a protein-coding gene that encodes for the immunoglobulin G2b (IgG2b) subclass of rat antibodies. IgG2b is a major subclass of rat immunoglobulins that plays a crucial role in the immune response by neutralizing pathogens and promoting opsonization. Rat IgG2b is commonly used in research applications, such as in immunohistochemistry, flow cytometry, and Western blotting.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: IgG2; Immunoglobulin G; Immunoglobulin G2; ImmunoglobulinG; ImmunoglobulinG2
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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