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Invitrogen
Rat IgG2b kappa Isotype Control (eB149/10H5), Brilliant Violet™ 650, eBioscience™
FIGURE: 1 / 1
Rat IgG2b kappa Isotype Control (416-4031-81) in Flow
BALB/c mouse bone marrow cells were stained with CD45R (B220) Monoclonal Antibody, FITC (Product # 11-0452-82) and staining buffer (autofluorescence) (left) or 1.0 µg of Rat IgG2b kappa Isotype Control, Brilliant Violet 650 (right). Total viable cells were used for analysis, as determined by LIVE/DEAD™ Fixable Far Red Dead Cell Stain Kit (Product # L34974).
Product Details
416-4031-81
Applications
Tested Dilution
Flow Cytometry (Flow)
Assay-dependent
Control (Ctrl)
Assay-dependent
Product Specifications
Host/Isotype
Rat
/ IgG2b, kappa
Class
Monoclonal
Type
Isotype Control
Clone
eB149/10H5
Conjugate
Excitation/Emission Max
407/646 nm
View spectra
Form
Liquid
Concentration
0.2 mg/mL
Purification
Affinity chromatography
Storage buffer
PBS, pH 7.2, with BSA
Contains
0.09% sodium azide
Storage conditions
4° C, store in dark, DO NOT FREEZE!
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2925693
Product Specific Information
Description: The rat IgG2b kappa monoclonal antibody is useful as an isotype control immunoglobulin.
Applications Reported: This eB149/10H5 antibody has been reported for use in flow cytometric analysis.
Applications Tested: This eB149/10H5 antibody has been tested by flow cytometric analysis of normal human peripheral blood and mouse bone marrow cells. Use at the same concentration as the experimental antibody.
Brilliant Violet™ 650 (BV650) is a tandem dye that emits at 649 nm and is intended for use on cytometers equipped with a violet (405 nm) laser. Please make sure that your instrument is capable of detecting this fluorochrome.
When using two or more Super Bright, Brilliant Violet™, Brilliant Ultra Violet™, or other polymer dye-conjugated antibodies in a staining panel, it is recommended to use Super Bright Complete Staining Buffer (Product # SB-4401-42) or Brilliant Stain Buffer™ (Product # 00-4409-75) to minimize any non-specific polymer interactions. Please refer to the datasheet for Super Bright Staining Buffer or Brilliant Stain Buffer for more information.
Light sensitivity: This tandem dye is sensitive to photo-induced oxidation. Please protect this vial and stained samples from light.
Fixation: Samples can be stored in IC Fixation Buffer (Product # 00-8222-49) (100 µL of cell sample + 100 µL of IC Fixation Buffer) or 1-step Fix/Lyse Solution (Product # 00-5333-54) for up to 3 days in the dark at 4°C with minimal impact on brightness and FRET efficiency/compensation. Some generalizations regarding fluorophore performance after fixation can be made, but clone-specific performance should be determined empirically.
Our internal testing suggests that Brilliant Violet™ 650 (BV650) is not compatible with methanol-based fixation.
Excitation: 407 nm; Emission: 649 nm; Laser: Violet Laser.
BRILLIANT VIOLET™ is a trademark or registered trademark of Becton, Dickinson and Company or its affiliates, and is used under license. Powered by Sirigen™.
Target Information
Rat IgG2b is a protein-coding gene that encodes for the immunoglobulin G2b (IgG2b) subclass of rat antibodies. IgG2b is a major subclass of rat immunoglobulins that plays a crucial role in the immune response by neutralizing pathogens and promoting opsonization. Rat IgG2b is commonly used in research applications, such as in immunohistochemistry, flow cytometry, and Western blotting.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: IgG2; Immunoglobulin G; Immunoglobulin G2; ImmunoglobulinG; ImmunoglobulinG2
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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