Invitrogen

CD278 (ICOS) Monoclonal Antibody (C398.4A), FITC, eBioscience™

CD278 (ICOS) Antibody in Flow Cytometry (Flow)

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CD278 (ICOS) Antibody (11-9949-82) in Flow

Staining of 3-day Con A-stimulated C57Bl/6 splenocytes with 0.25 µg of Armenian Hamster IgG Isotype Control FITC (Product # 11-4888-81) (blue histogram) or 0.25 µg of Anti-Mouse/Rat CD278 (ICOS) FITC (purple histogram). Total viable cells were used for analysis.

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Product Details

11-9949-82

Applications

Tested Dilution

Publications

Flow Cytometry (Flow)

0.5 µg/test

View 17 publications 17 publications

Product Specifications

Species Reactivity

Mouse, Rhesus monkey, Rat

Published species

Mouse

Host/Isotype

Armenian hamster / IgG

Recommended Isotype Control

Armenian Hamster IgG Isotype Control (eBio299Arm), FITC, eBioscience™

Class

Monoclonal

Type

Antibody

Clone

C398.4A

Conjugate

FITC FITC FITC

Excitation/Emission Max

498/517 nm View spectra

Form

Liquid

Concentration

0.5 mg/mL

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.2

Contains

0.09% sodium azide

Storage conditions

4° C, store in dark, DO NOT FREEZE!

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_465458

Product Specific Information

Description: The C398.4A monoclonal antibody reacts with ICOS (Inducible Costimulatory molecule), also known as H4/CRP-1/AILIM, and cross-reacts with mouse and rat ICOS. ICOS is a T cell specific activation molecule and a third member of the CD28/CTLA-4 family. A dimer of 47-57 kDa, ICOS is expressed on activated T cells, has potent costimulatory activity for T cell activation and proliferation and is required for humoral immune response. ICOS binds to its ligand on activated APC including B cells called B7h/B7RP-1, is thought to play a protective role in inflammatory autoimmune diseases and be involved in the development of Th2 cells. C398.4A is reported to display strong costimulation of proliferation of T cell clones, intermediate costimulation on activated T cells, and weak costimulation on fresh resting T cells. It is a good costimulator of IL-10 production especially from pre-activated cells that express high level of H4/ICOS. The epitope recognized by C398.4A is different from that bound by the ICOSL (i.e., C398.4A does not block binding of ICOSL).

Applications Reported: The C398.4A antibody has been reported for use in flow cytometric analysis.

Applications Tested: The C398.4A antibody has been tested by flow cytometric analysis of Con A-stimulated mouse splenocytes. This can be used at less than or equal to 0.5 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

Excitation: 488 nm; Emission: 520 nm; Laser: Blue Laser.

Filtration: 0.2 µm post-manufacturing filtered.

Target Information

ICOS (CD278) encodes a protein that is a member of the CD28 and CTLA-4 cell-surface receptor family. It functions as a homodimer and has a crucial role in cell-cell signaling, immune responses, and the regulation of cell proliferation. ICOS has been linked to various disorders, including Immunodeficiency, Common Variable, 1 and Common Variable Immunodeficiency.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Product References

TRAF6 controls T cell homeostasis by maintaining the equilibrium of MALT1 scaffolding and protease functions.

Frontiers in immunology

O'Neill TJ,Gewies A,Seeholzer T,Krappmann D

11-9949-82 was used in Flow Cytometry to demonstrate that TRAF6 deletion or MALT1 paracaspase inactivation are highly interdependent in causing the distinct immune pathologies.

Mon Feb 13 00:00:00 EST 2023

HSF1 promotes CD69⁺ Treg differentiation to inhibit colitis progression.

Theranostics

Yu L,Zhou B,Zhu Y,Li L,Zhong Y,Zhu L,Wang H,Chen H,Xu J,Guo T,Feng L,Wang X,Cai Z,Wang J,Jin H

Published figure using CD278 (ICOS) monoclonal antibody (Product # 11-9949-82) in Flow Cytometry

Tue Apr 18 00:00:00 EDT 2023

Organoids capture tissue-specific innate lymphoid cell development in mice and humans.

Cell reports

Jowett GM,Read E,Roberts LB,Coman D,Vilà González M,Zabinski T,Niazi U,Reis R,Trieu TJ,Danovi D,Gentleman E,Vallier L,Curtis MA,Lord GM,Neves JF

Published figure using CD278 (ICOS) monoclonal antibody (Product # 11-9949-82) in Flow Cytometry

Tue Aug 30 00:00:00 EDT 2022

Chronic stress physically spares but functionally impairs innate-like invariant T cells.

Cell reports

Rudak PT,Choi J,Parkins KM,Summers KL,Jackson DN,Foster PJ,Skaro AI,Leslie K,McAlister VC,Kuchroo VK,Inoue W,Lantz O,Haeryfar SMM

11-9949-82 was used in Flow Cytometry to report that long-term stress surprisingly abrogates both T helper 1 (TH1)- and TH2-type responses orchestrated by invariant natural killer T (iNKT) cells.

Tue Apr 13 00:00:00 EDT 2021

Dermal Adipocyte Lipolysis and Myofibroblast Conversion Are Required for Efficient Skin Repair.

Cell stem cell

Shook BA,Wasko RR,Mano O,Rutenberg-Schoenberg M,Rudolph MC,Zirak B,Rivera-Gonzalez GC,López-Giráldez F,Zarini S,Rezza A,Clark DA,Rendl M,Rosenblum MD,Gerstein MB,Horsley V

11-9949-82 was used in Flow Cytometry to identify an essential role for adipocyte lipolysis in regulating inflammation and repair after injury in skin.

Thu Jun 04 00:00:00 EDT 2020

Toxin-Triggered Interleukin-1 Receptor Signaling Enables Early-Life Discrimination of Pathogenic versus Commensal Skin Bacteria.

Cell host & microbe

Leech JM,Dhariwala MO,Lowe MM,Chu K,Merana GR,Cornuot C,Weckel A,Ma JM,Leitner EG,Gonzalez JR,Vasquez KS,Diep BA,Scharschmidt TC

11-9949-82 was used in Flow Cytometry to study how skin colonization by commensal Staphylococcus epidermidis facilitates immune tolerance preferentially in neonates via induction of antigen-specific regulatory T cells.

Wed Dec 11 00:00:00 EST 2019

Toxin-Triggered Interleukin-1 Receptor Signaling Enables Early-Life Discrimination of Pathogenic versus Commensal Skin Bacteria.

Cell host & microbe

Leech JM,Dhariwala MO,Lowe MM,Chu K,Merana GR,Cornuot C,Weckel A,Ma JM,Leitner EG,Gonzalez JR,Vasquez KS,Diep BA,Scharschmidt TC

Wed Dec 11 00:00:00 EST 2019

Pulsatile MEK Inhibition Improves Anti-tumor Immunity and T Cell Function in Murine Kras Mutant Lung Cancer.

Cell reports

Choi H,Deng J,Li S,Silk T,Dong L,Brea EJ,Houghton S,Redmond D,Zhong H,Boiarsky J,Akbay EA,Smith PD,Merghoub T,Wong KK,Wolchok JD

11-9949 was used in Flow cytometry/Cell sorting to set the foundation and show the importance of a combinatorial therapeutic strategy using pulsatile targeted therapy together with immunotherapy to optimally enhance tumor delay and promote long-term anti-tumor immunity.

Tue Apr 16 00:00:00 EDT 2019

Non-conventional Inhibitory CD4⁺Foxp3^-PD-1^hi T Cells as a Biomarker of Immune Checkpoint Blockade Activity.

Cancer cell

Zappasodi R,Budhu S,Hellmann MD,Postow MA,Senbabaoglu Y,Manne S,Gasmi B,Liu C,Zhong H,Li Y,Huang AC,Hirschhorn-Cymerman D,Panageas KS,Wherry EJ,Merghoub T,Wolchok JD

11-9949-82 was used in Flow Cytometry to provide evidence that mouse and human circulating and intra-tumor 4PD1hi inhibit T cell functions in a PD-1/PD-L1 dependent fashion and resemble follicular helper T cell (TFH)-like cells.

Mon Jun 11 00:00:00 EDT 2018

The Kinase Complex mTOR Complex 2 Promotes the Follicular Migration and Functional Maturation of Differentiated Follicular Helper CD4⁺ T Cells During Viral Infection.

Frontiers in immunology

Hao Y,Wang Y,Liu X,Yang X,Wang P,Tian Q,Bai Q,Chen X,Li Z,Wu J,Xie Z,Zhou X,Zhou Y,Yin Z,Wu Y,Ye L

Published figure using CD278 (ICOS) monoclonal antibody (Product # 11-9949-82) in Flow Cytometry

Tue Jul 30 00:00:00 EDT 2019

Differential cell-intrinsic regulations of germinal center B and T cells by miR-146a and miR-146b.

Nature communications

Cho S,Lee HM,Yu IS,Choi YS,Huang HY,Hashemifar SS,Lin LL,Chen MC,Afanasiev ND,Khan AA,Lin SW,Rudensky AY,Crotty S,Lu LF

Published figure using CD278 (ICOS) monoclonal antibody (Product # 11-9949-82) in Flow Cytometry

Mon Jul 16 00:00:00 EDT 2018

MicroRNA-155 Protects Group 2 Innate Lymphoid Cells From Apoptosis to Promote Type-2 Immunity.

Frontiers in immunology

Knolle MD,Chin SB,Rana BMJ,Englezakis A,Nakagawa R,Fallon PG,Git A,McKenzie ANJ

Published figure using CD278 (ICOS) monoclonal antibody (Product # 11-9949-82) in Flow Cytometry

Mon Sep 30 00:00:00 EDT 2019

Regulatory T Cells in Skin Facilitate Epithelial Stem Cell Differentiation.

Cell

Ali N,Zirak B,Rodriguez RS,Pauli ML,Truong HA,Lai K,Ahn R,Corbin K,Lowe MM,Scharschmidt TC,Taravati K,Tan MR,Ricardo-Gonzalez RR,Nosbaum A,Bertolini M,Liao W,Nestle FO,Paus R,Cotsarelis G,Abbas AK,Rosenblum MD

11-9949 was used in Flow cytometry/Cell sorting to study the role of regulatory T cells in hair follicles and hair follicle stem cell biology.

Thu Jun 01 00:00:00 EDT 2017

Commensal Microbes and Hair Follicle Morphogenesis Coordinately Drive Treg Migration into Neonatal Skin.

Cell host & microbe

Scharschmidt TC,Vasquez KS,Pauli ML,Leitner EG,Chu K,Truong HA,Lowe MM,Sanchez Rodriguez R,Ali N,Laszik ZG,Sonnenburg JL,Millar SE,Rosenblum MD

Wed Apr 12 00:00:00 EDT 2017

Interleukin-21 promotes germinal center reaction by skewing the follicular regulatory T cell to follicular helper T cell balance in autoimmune BXD2 mice.

Arthritis & rheumatology (Hoboken, N.J.)

Ding Y,Li J,Yang P,Luo B,Wu Q,Zajac AJ,Wildner O,Hsu HC,Mountz JD

11-9949 was used in Flow cytometry/Cell sorting to indicate that high levels of IL-21 selectively enhance Tfh cell differentiation but inhibit Tfr cell commitment and the suppressive function of Tfr cells on Tfh cells and B cells.

Mon Sep 01 00:00:00 EDT 2014

Cytokine therapy with interleukin-2/anti-interleukin-2 monoclonal antibody complexes expands CD4+CD25+Foxp3+ regulatory T cells and attenuates development and progression of atherosclerosis.

Circulation

Dinh TN,Kyaw TS,Kanellakis P,To K,Tipping P,Toh BH,Bobik A,Agrotis A

11-9949 was used in Flow cytometry/Cell sorting to examine the capacity of a modified IL-2/anti-IL-2 monoclonal antibody treatment to expand Tregs and attenuate atherosclerosis in mice.

Tue Sep 04 00:00:00 EDT 2012

Murine CD4⁺CD25⁻ cells activated in vitro with PMA/ionomycin and anti-CD3 acquire regulatory function and ameliorate experimental colitis in vivo.

BMC gastroenterology

Majowicz A,van der Marel S,te Velde AA,Meijer SL,Petry H,van Deventer SJ,Ferreira V

11-9949 was used in Flow cytometry/Cell sorting to identify a new method to generate in vitro iTreg cells (TregPMA cells) with demonstrated functionality in vivo.

Mon Dec 03 00:00:00 EST 2012

Transient blockade of the inducible costimulator pathway generates long-term tolerance to factor VIII after nonviral gene transfer into hemophilia A mice.

Blood

Peng B,Ye P,Blazar BR,Freeman GJ,Rawlings DJ,Ochs HD,Miao CH

11-9949 was used in Flow cytometry/Cell sorting to study the formation of inhibitory antibodies during treatment of hemophilia, showing that transient blockade of the inducible costimulator pathway generates long-term tolerance to factor VIII.

Mon Sep 01 00:00:00 EDT 2008

Bioinformatics

Protein Aliases: Activation-inducible lymphocyte immunomediatory molecule; CCLP; CD278; CD28 and CTLA-4-like protein; CD28-related protein 1; CRP-1; inducible T-cell co-stimulator; Inducible T-cell costimulator

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Gene Aliases: AILIM; CCLP; CRP-1; H4; Icos; Ly115

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UniProt ID: (Rat) Q9R1T7, (Mouse) Q9WVS0

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Entrez Gene ID: (Rhesus monkey) 705788, (Rat) 64545, (Mouse) 54167

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Function(s)

molecular_function protein binding

Process(es)

T cell tolerance induction single organismal cell-cell adhesion T cell costimulation

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

Learn more

CD278 (ICOS) Monoclonal Antibody (C398.4A), FITC, eBioscience™

CD278 (ICOS) Antibody (11-9949-82) in Flow

Product Details

11-9949-82

Flow Cytometry (Flow)

Species Reactivity

Published species

Host/Isotype

Recommended Isotype Control

Class

Type

Clone

Conjugate

Excitation/Emission Max

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

How to use the Panel Builder

Bioinformatics

Performance Guarantee

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