Invitrogen

CD278 (ICOS) Monoclonal Antibody (C398.4A), PE, eBioscience™

CD278 (ICOS) Antibody in Flow Cytometry (Flow)

FIGURE: 1 / 7

CD278 (ICOS) Antibody (12-9949-81) in Flow

Swiss Webster mouse splenocytes were stimulated for 72 hours with Con A (Product # 00-4978-03) and then stained with CD25 Monoclonal Antibody, eFluor 450 (Product # 48-0251-82) and 0.03 µg of Armenian Hamster IgG Isotype Control, PE (Product # 12-4888-83) (left) or 0.03 µg of ICOS Monoclonal Antibody, PE (right). Total viable cells were used for analysis, as determined by Fixable Viability Dye eFluor 780 (Product # 65-0865-18).

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Product Details

12-9949-81

Applications

Tested Dilution

Publications

Flow Cytometry (Flow)

0.125 µg/test

View 15 publications 15 publications

Product Specifications

Species Reactivity

Mouse, Rhesus monkey, Rat

Published species

Human, Mouse

Host/Isotype

Armenian hamster / IgG

Recommended Isotype Control

Armenian Hamster IgG Isotype Control (eBio299Arm), PE, eBioscience™

Class

Monoclonal

Type

Antibody

Clone

C398.4A

Conjugate

PE PE PE

Excitation/Emission Max

565/576 nm View spectra

Form

Liquid

Concentration

0.2 mg/mL

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.2

Contains

0.09% sodium azide

Storage conditions

4° C, store in dark, DO NOT FREEZE!

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_466277

Product Specific Information

Description: The C398.4A monoclonal antibody reacts with ICOS (Inducible Costimulatory molecule), also known as H4/CRP-1/AILIM, and cross-reacts with mouse and rat ICOS. ICOS is a T cell specific activation molecule and a third member of the CD28/CTLA-4 family. A dimer of 47-57 kDa, ICOS is expressed on activated T cells, has potent costimulatory activity for T cell activation and proliferation and is required for humoral immune response. ICOS binds to its ligand on activated APC including B cells called B7h/B7RP-1, is thought to play a protective role in inflammatory autoimmune diseases and be involved in the development of Th2 cells. C398.4A is reported to display strong costimulation of proliferation of T cell clones, intermediate costimulation on activated T cells, and weak costimulation on fresh resting T cells. It is a good costimulator of IL-10 production especially from pre-activated cells that express high level of H4/ICOS. The epitope recognized by C398.4A is different from that bound by the ICOSL (i.e., C398.4A does not block binding of ICOSL).

Applications Reported: The C398.4A antibody has been reported for use in flow cytometric analysis.

Applications Tested: This C398.4A antibody has been tested by flow cytometric analysis of Con A-stimulated mouse splenocytes. This can be used at less than or equal to 0.125 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

Excitation: 488-561 nm; Emission: 578 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser.

Filtration: 0.2 µm post-manufacturing filtered.

Target Information

ICOS (CD278) encodes a protein that is a member of the CD28 and CTLA-4 cell-surface receptor family. It functions as a homodimer and has a crucial role in cell-cell signaling, immune responses, and the regulation of cell proliferation. ICOS has been linked to various disorders, including Immunodeficiency, Common Variable, 1 and Common Variable Immunodeficiency.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Product References

HSF1 promotes CD69⁺ Treg differentiation to inhibit colitis progression.

Theranostics

Yu L,Zhou B,Zhu Y,Li L,Zhong Y,Zhu L,Wang H,Chen H,Xu J,Guo T,Feng L,Wang X,Cai Z,Wang J,Jin H

Published figure using CD278 (ICOS) monoclonal antibody (Product # 12-9949-81) in Flow Cytometry

Tue Apr 18 00:00:00 EDT 2023

CARD11 gain-of-function mutation drives cell-autonomous accumulation of PD-1⁺ ICOS^high activated T cells, T-follicular, T-regulatory and T-follicular regulatory cells.

Frontiers in immunology

Masle-Farquhar E,Jeelall Y,White J,Bier J,Deenick EK,Brink R,Horikawa K,Goodnow CC

12-9949-81 was used in Flow cytometry/Cell sorting to reveal CARD11 as an important, cell-autonomous positive regulator of TFH, TREG and TFR cells.

Mon Mar 27 00:00:00 EDT 2023

Phospholipid scramblase-1 regulates innate type 2 inflammation in mouse lungs via CRTH2-dependent mechanisms.

The Journal of clinical investigation

Hernandez-Gutierrez A,Majid S,Eberle A,Choi A,Sorkhdini P,Yang D,Yang AX,Norbrun C,He CH,Lee CM,Lee CG,Elias JA,Zhou Y

12-9949-81 was used in Flow Cytometry to demonstrate that PLSCR1 played an essential role in the pathogenesis of ILC2 responses, providing critical insights into biology and disease pathogenesis and identifying targets that can be manipulated in attempts to control IT2IR in chronic diseases such as asthma.

Tue Aug 01 00:00:00 EDT 2023

TRAF6 controls T cell homeostasis by maintaining the equilibrium of MALT1 scaffolding and protease functions.

Frontiers in immunology

O'Neill TJ,Gewies A,Seeholzer T,Krappmann D

Published figure using CD278 (ICOS) monoclonal antibody (Product # 12-9949-81) in Flow Cytometry

Mon Feb 13 00:00:00 EST 2023

Organoids capture tissue-specific innate lymphoid cell development in mice and humans.

Cell reports

Jowett GM,Read E,Roberts LB,Coman D,Vilà González M,Zabinski T,Niazi U,Reis R,Trieu TJ,Danovi D,Gentleman E,Vallier L,Curtis MA,Lord GM,Neves JF

Published figure using CD278 (ICOS) monoclonal antibody (Product # 12-9949-81) in Flow Cytometry

Tue Aug 30 00:00:00 EDT 2022

SARS-CoV-2 infection paralyzes cytotoxic and metabolic functions of the immune cells.

Heliyon

Singh Y,Trautwein C,Fendel R,Krickeberg N,Berezhnoy G,Bissinger R,Ossowski S,Salker MS,Casadei N,Riess O

12-9949-81 was used in Flow Cytometry to study the effects of SARS-CoV-2 virus infection on immune and metabolic functions in human cells.

Tue Jun 01 00:00:00 EDT 2021

MEF2D sustains activation of effector Foxp3+ Tregs during transplant survival and anticancer immunity.

The Journal of clinical investigation

Di Giorgio E,Wang L,Xiong Y,Akimova T,Christensen LM,Han R,Samanta A,Trevisanut M,Bhatti TR,Beier UH,Hancock WW

12-9949 was used in Flow cytometry/Cell sorting to study the role played by MEF2D in sustaining effector Foxp3+ Treg functions without abrogating their basal actions suggests its suitability for drug discovery efforts in cancer therapy.

Tue Dec 01 00:00:00 EST 2020

Subsets of ILC3-ILC1-like cells generate a diversity spectrum of innate lymphoid cells in human mucosal tissues.

Nature immunology

Cella M,Gamini R,Sécca C,Collins PL,Zhao S,Peng V,Robinette ML,Schettini J,Zaitsev K,Gordon W,Bando JK,Yomogida K,Cortez V,Fronick C,Fulton R,Lin LL,Gilfillan S,Flavell RA,Shan L,Artyomov MN,Bowman M,Oltz EM,Jelinsky SA,Colonna M

12-9949 was used in Flow cytometry/Cell sorting to demonstrate that innate lymphoid cells (ILCs) undergo conversion from ILC3s into ILC1-like cells in human tissues in vivo, requiring tissue factors and Aiolos in the process.

Thu Aug 01 00:00:00 EDT 2019

The Kinase Complex mTOR Complex 2 Promotes the Follicular Migration and Functional Maturation of Differentiated Follicular Helper CD4⁺ T Cells During Viral Infection.

Frontiers in immunology

Hao Y,Wang Y,Liu X,Yang X,Wang P,Tian Q,Bai Q,Chen X,Li Z,Wu J,Xie Z,Zhou X,Zhou Y,Yin Z,Wu Y,Ye L

Published figure using CD278 (ICOS) monoclonal antibody (Product # 12-9949-81) in Flow Cytometry

Tue Jul 30 00:00:00 EDT 2019

Differential cell-intrinsic regulations of germinal center B and T cells by miR-146a and miR-146b.

Nature communications

Cho S,Lee HM,Yu IS,Choi YS,Huang HY,Hashemifar SS,Lin LL,Chen MC,Afanasiev ND,Khan AA,Lin SW,Rudensky AY,Crotty S,Lu LF

Published figure using CD278 (ICOS) monoclonal antibody (Product # 12-9949-81) in Flow Cytometry

Mon Jul 16 00:00:00 EDT 2018

MicroRNA-155 Protects Group 2 Innate Lymphoid Cells From Apoptosis to Promote Type-2 Immunity.

Frontiers in immunology

Knolle MD,Chin SB,Rana BMJ,Englezakis A,Nakagawa R,Fallon PG,Git A,McKenzie ANJ

Published figure using CD278 (ICOS) monoclonal antibody (Product # 12-9949-81) in Flow Cytometry

Mon Sep 30 00:00:00 EDT 2019

Ablation of Transcription Factor IRF4 Promotes Transplant Acceptance by Driving Allogenic CD4⁺ T Cell Dysfunction.

Immunity

Wu J,Zhang H,Shi X,Xiao X,Fan Y,Minze LJ,Wang J,Ghobrial RM,Xia J,Sciammas R,Li XC,Chen W

12-9949-81 was used in Flow Cytometry to demonstrate that interferon regulatory factor 4 was a key transcriptional determinant controlling T cell responses during transplantation.

Tue Dec 19 00:00:00 EST 2017

IL-21 restricts T follicular regulatory T cell proliferation through Bcl-6 mediated inhibition of responsiveness to IL-2.

Nature communications

Jandl C,Liu SM,Cañete PF,Warren J,Hughes WE,Vogelzang A,Webster K,Craig ME,Uzel G,Dent A,Stepensky P,Keller B,Warnatz K,Sprent J,King C

12-9949 was used in Flow cytometry/Cell sorting to identify a mechanism by which IL-21 reinforces humoural immunity by influencing the phenotype of T follicular cells to limit their expansion.

Fri Mar 17 00:00:00 EDT 2017

Partial restoration of T-cell function in aged mice by in vitro blockade of the PD-1/ PD-L1 pathway.

Aging cell

Lages CS,Lewkowich I,Sproles A,Wills-Karp M,Chougnet C

12-9949 was used in Flow cytometry/Cell sorting to suggest that blockade of the PD-1/PD-L1 is not likely to be efficient at restoring exhausted T-cell responses in aged hosts.

Fri Oct 01 00:00:00 EDT 2010

Expression and function of inducible costimulator on peripheral blood T cells in patients with systemic lupus erythematosus.

Rheumatology (Oxford, England)

Yang JH,Zhang J,Cai Q,Zhao DB,Wang J,Guo PE,Liu L,Han XH,Shen Q

12-9949 was used in Flow cytometry/Cell sorting to show that the abnormality of ICOS costimulation in SLE patients may play an immunopathological role in pathogenesis.

Sat Oct 01 00:00:00 EDT 2005

Bioinformatics

Protein Aliases: Activation-inducible lymphocyte immunomediatory molecule; CCLP; CD278; CD28 and CTLA-4-like protein; CD28-related protein 1; CRP-1; inducible T-cell co-stimulator; Inducible T-cell costimulator

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Gene Aliases: AILIM; CCLP; CRP-1; H4; Icos; Ly115

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UniProt ID: (Rat) Q9R1T7, (Mouse) Q9WVS0

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Entrez Gene ID: (Rhesus monkey) 705788, (Rat) 64545, (Mouse) 54167

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Function(s)

molecular_function protein binding

Process(es)

T cell tolerance induction single organismal cell-cell adhesion T cell costimulation

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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CD278 (ICOS) Monoclonal Antibody (C398.4A), PE, eBioscience™

CD278 (ICOS) Antibody (12-9949-81) in Flow

Product Details

12-9949-81

Flow Cytometry (Flow)

Species Reactivity

Published species

Host/Isotype

Recommended Isotype Control

Class

Type

Clone

Conjugate

Excitation/Emission Max

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

How to use the Panel Builder

Bioinformatics

Performance Guarantee

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