Invitrogen

CD279 (PD-1) Monoclonal Antibody (RMP1-30), FITC, eBioscience™

CD279 (PD-1) Antibody in Flow Cytometry (Flow)

FIGURE: 1 / 19

CD279 (PD-1) Antibody (11-9981-82) in Flow

Staining of 3-day Anti-Mouse CD3 and Anti-Mouse CD28 Functional Grade Purified (Product # 16-0031-82 and Product # 16-0281-82)-stimulated C57Bl/6 splenocytes with 0.25 µg of Rat IgG2b K Isotype Control FITC (Product # 11-4031-82) (blue histogram) or 0.25 µg of Anti-Mouse CD279 (PD-1) FITC (purple histogram). Total viable cells, as determined by Fixable Viability Dye eFluor® 450 (Product # 65-0863-14), were used for analysis.

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Product Details

11-9981-82

Applications

Tested Dilution

Publications

Flow Cytometry (Flow)

0.5 µg/test

View 19 publications 19 publications

Product Specifications

Species Reactivity

Mouse

Published species

Human, Mouse

Host/Isotype

Rat / IgG2b, kappa

Recommended Isotype Control

Rat IgG2b kappa Isotype Control (eB149/10H5), FITC, eBioscience™

Class

Monoclonal

Type

Antibody

Clone

RMP1-30

Conjugate

FITC FITC FITC

Excitation/Emission Max

498/517 nm View spectra

Form

Liquid

Concentration

0.5 mg/mL

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.2

Contains

0.09% sodium azide

Storage conditions

4° C, store in dark, DO NOT FREEZE!

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_465467

Product Specific Information

Description: The RMP1-30 antibody reacts with mouse PD-1 (programmed death-1), a 55 kDa member of the Ig superfamily. PD-1 contains the immunoreceptor tyrosine-based inhibitory motif (ITIM) and plays a key role in peripheral tolerance and autoimmune disease in mice. PD-1 is expressed mainly on activated T and B lymphocytes. Two novel B7 Family members have been identified as PD-1 ligands, PD-L1 (B7-H1) and PD-L2 (B7-DC). Evidence reported to date suggests overlapping functions for these ligands and their constitutive expression on some normal tissues and upregulation on activated antigen-presenting cells. RMP1-30 does not block the binding of either B7-H1-Ig or B7-DC-Ig to PD-1 transfectants.

Applications Reported: The RMP1-30 antibody has been reported for use in flow cytometric analysis.

Applications Tested: The RMP1-30 antibody has been tested by flow cytometric analysis of Con A-stimulated mouse splenocytes. This can be used at less than or equal to 0.5 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

Excitation: 488 nm; Emission: 520 nm; Laser: Blue Laser.

Filtration: 0.2 µm post-manufacturing filtered.

Target Information

Cell-mediated immune responses are initiated by T lymphocytes that are themselves stimulated by cognate peptides bound to MHC molecules on antig en-presenting cells (APC). T-cell activation is generally self-limited as activated T cells express receptors such as PD-1 (also known as PDCD-1) that mediate inhibitory signals from the APC. PD-1 can bind two different but related ligands, PDL-1 and PDL-2. Upon binding to either of these ligands, signals generated by PD-1 inhibit the activation of the immune response in the absence of "danger signals" such as LPS or other molecules associated with bacteria or other pathogens. Evidence for this is seen in PD1-null mice who exhibit hyperactivated immune systems and autoimmune diseases. Despite its predicted molecular weight, PD-1 often migrates at higher molecular weight in SDS-PAGE.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Product References

BRAF D594A mutation defines a unique biological and immuno-modulatory subgroup associated with functional CD8⁺ T cell infiltration in colorectal cancer.

Journal of translational medicine

Li W,Zhao C,Li W,Gong Y,Ma K,Lu Y,Liu X,Zhang L,Guo F

Published figure using CD279 (PD-1) monoclonal antibody (Product # 11-9981-82) in Flow Cytometry

Wed Oct 18 00:00:00 EDT 2023

Oncolytic virus M1 functions as a bifunctional checkpoint inhibitor to enhance the antitumor activity of DC vaccine.

Cell reports. Medicine

Dan J,Cai J,Zhong Y,Wang C,Huang S,Zeng Y,Fan Z,Xu C,Hu L,Zhang J,Hu J,Liu Y,Su X,Zhu W,Yan G,Liang J,Lin Y

Published figure using CD279 (PD-1) monoclonal antibody (Product # 11-9981-82) in Flow Cytometry

Tue Oct 17 00:00:00 EDT 2023

Prolidase Deficiency Causes Spontaneous T Cell Activation and Lupus-like Autoimmunity.

Journal of immunology (Baltimore, Md. : 1950)

Hodgson R,Crockford TL,Bhandari A,Kepple JD,Back J,Cawthorne E,Abeler-Dörner L,Laing AG,Clare S,Speak A,Adams DJ,Dougan G,Hayday AC,Deobagkar-Lele M,Cornall RJ,Bull KR

11-9981-82 was used in Flow Cytometry to address the question of causation and show that Pepd-null mice have increased antinuclear autoantibodies and raised serum IgA, accompanied by kidney immune complex deposition, consistent with a systemic lupus erythematosus-like disease.

Wed Mar 01 00:00:00 EST 2023

Breast cancer cell-derived extracellular vesicles promote CD8⁺ T cell exhaustion via TGF-β type II receptor signaling.

Nature communications

Xie F,Zhou X,Su P,Li H,Tu Y,Du J,Pan C,Wei X,Zheng M,Jin K,Miao L,Wang C,Meng X,van Dam H,Ten Dijke P,Zhang L,Zhou F

Published figure using CD279 (PD-1) monoclonal antibody (Product # 11-9981-82) in Flow Cytometry

Mon Aug 01 00:00:00 EDT 2022

Breast cancer cell-derived extracellular vesicles promote CD8⁺ T cell exhaustion via TGF-β type II receptor signaling.

Nature communications

Xie F,Zhou X,Su P,Li H,Tu Y,Du J,Pan C,Wei X,Zheng M,Jin K,Miao L,Wang C,Meng X,van Dam H,Ten Dijke P,Zhang L,Zhou F

Published figure using CD279 (PD-1) monoclonal antibody (Product # 11-9981-82) in Flow Cytometry

Mon Aug 01 00:00:00 EDT 2022

Functional assessment of the cell-autonomous role of NADase CD38 in regulating CD8⁺ T cell exhaustion.

iScience

Ma K,Sun L,Shen M,Zhang X,Xiao Z,Wang J,Liu X,Jiang K,Xiao-Feng Qin F,Guo F,Zhang B,Zhang L

Published figure using CD279 (PD-1) monoclonal antibody (Product # 11-9981-82) in Flow Cytometry

Fri May 20 00:00:00 EDT 2022

Translation factor eIF5a is essential for IFNγ production and cell cycle regulation in primary CD8⁺ T lymphocytes.

Nature communications

Tan TCJ,Kelly V,Zou X,Wright D,Ly T,Zamoyska R

11-9981-82 was used in Flow cytometry/Cell sorting to show that the function of the elongation factor, eIF5a, is regulated dynamically in naive CD8+ T cells upon activation by post-translational modification, whereupon it facilitates translation of specific subsets of proteins.

Sat Dec 17 00:00:00 EST 2022

CXCL10 chemokine regulates heterogeneity of the CD8⁺ T cell response and viral set point during chronic infection.

Immunity

Ozga AJ,Chow MT,Lopes ME,Servis RL,Di Pilato M,Dehio P,Lian J,Mempel TR,Luster AD

11-9981-82 was used in Flow cytometry/Cell sorting to find that the chemokine receptor CXCR3 is highly expressed on viral-specific stem-like CD8+ T cells and that one of its ligands, CXCL10, regulates the persistence and heterogeneity of responding CD8+ T cells in spleens of mice chronically infected with lymphocytic choriomeningitis virus.

Tue Jan 11 00:00:00 EST 2022

M2 macrophage-derived extracellular vesicles facilitate CD8+T cell exhaustion in hepatocellular carcinoma via the miR-21-5p/YOD1/YAP/β-catenin pathway.

Cell death discovery

Pu J,Xu Z,Nian J,Fang Q,Yang M,Huang Y,Li W,Ge B,Wang J,Wei H

Published figure using CD279 (PD-1) monoclonal antibody (Product # 11-9981-82) in Flow Cytometry

Fri Jul 16 00:00:00 EDT 2021

Combination of vasculature targeting, hypofractionated radiotherapy, and immune checkpoint inhibitor elicits potent antitumor immune response and blocks tumor progression.

Journal for immunotherapy of cancer

Pierini S,Mishra A,Perales-Linares R,Uribe-Herranz M,Beghi S,Giglio A,Pustylnikov S,Costabile F,Rafail S,Amici A,Facciponte JG,Koumenis C,Facciabene A

Published figure using CD279 (PD-1) monoclonal antibody (Product # 11-9981-82) in Flow Cytometry

Mon Feb 01 00:00:00 EST 2021

Ovarian granulosa cell tumor characterization identifies FOXL2 as an immunotherapeutic target.

JCI insight

Pierini S,Tanyi JL,Simpkins F,George E,Uribe-Herranz M,Drapkin R,Burger R,Morgan MA,Facciabene A

Published figure using CD279 (PD-1) monoclonal antibody (Product # 11-9981-82) in Flow Cytometry

Thu Aug 20 00:00:00 EDT 2020

Creatine uptake regulates CD8 T cell antitumor immunity.

The Journal of experimental medicine

Di Biase S,Ma X,Wang X,Yu J,Wang YC,Smith DJ,Zhou Y,Li Z,Kim YJ,Clarke N,To A,Yang L

Published figure using CD279 (PD-1) monoclonal antibody (Product # 11-9981-82) in Flow Cytometry

Mon Dec 02 00:00:00 EST 2019

Localized Treatment with Oncolytic Adenovirus Delta-24-RGDOX Induces Systemic Immunity against Disseminated Subcutaneous and Intracranial Melanomas.

Clinical cancer research : an official journal of the American Association for Cancer Research

Jiang H,Shin DH,Nguyen TT,Fueyo J,Fan X,Henry V,Carrillo CC,Yi Y,Alonso MM,Collier TL,Yuan Y,Lang FF,Gomez-Manzano C

11-9981 was used in Flow cytometry/Cell sorting to show that localized intratumoral injection of Delta-24-RGDOX induces an in situ antovaccination of the treated melanoma, the effect of which changes the immune landscape of the treated mice, resulting in systemic immunity against disseminated s.c. and i.c. tumors.

Fri Nov 15 00:00:00 EST 2019

Autophagy Regulation of Metabolism Is Required for CD8⁺ T Cell Anti-tumor Immunity.

Cell reports

DeVorkin L,Pavey N,Carleton G,Comber A,Ho C,Lim J,McNamara E,Huang H,Kim P,Zacharias LG,Mizushima N,Saitoh T,Akira S,Beckham W,Lorzadeh A,Moksa M,Cao Q,Murthy A,Hirst M,DeBerardinis RJ,Lum JJ

11-9981 was used in Flow cytometry/Cell sorting to demonstrate a cell-autonomous role for autophagy in the regulation of CD8+ T-cell-mediated control of tumors.

Tue Apr 09 00:00:00 EDT 2019

The Kinase mTORC1 Promotes the Generation and Suppressive Function of Follicular Regulatory T Cells.

Immunity

Xu L,Huang Q,Wang H,Hao Y,Bai Q,Hu J,Li Y,Wang P,Chen X,He R,Li B,Yang X,Zhao T,Zhang Y,Wang Y,Ou J,Liang H,Wu Y,Zhou X,Ye L

11-9981-82 was used in Flow Cytometry to study the effect of mTORC1 on Tfr cell differentiation by activating the TCF-1-Bcl-6 axis during immunization or infection.

Tue Sep 19 00:00:00 EDT 2017

ILC2s regulate adaptive Th2 cell functions via PD-L1 checkpoint control.

The Journal of experimental medicine

Schwartz C,Khan AR,Floudas A,Saunders SP,Hams E,Rodewald HR,McKenzie ANJ,Fallon PG

11-9981 was used in Flow cytometry/Cell sorting to study how group 2 innate lymphoid cells regulate adaptive Th2 cell functions.

Mon Sep 04 00:00:00 EDT 2017

Antitumor adaptive immunity remains intact following inhibition of autophagy and antimalarial treatment.

The Journal of clinical investigation

Starobinets H,Ye J,Broz M,Barry K,Goldsmith J,Marsh T,Rostker F,Krummel M,Debnath J

11-9981 was used in Flow cytometry/Cell sorting to evaluate how autophagy inhibition impacts the antitumor immune response in immune-competent mouse models of melanoma and mammary cancer.

Thu Dec 01 00:00:00 EST 2016

Interdependent IL-7 and IFN-γ signalling in T-cell controls tumour eradication by combined α-CTLA-4+α-PD-1 therapy.

Nature communications

Shi LZ,Fu T,Guan B,Chen J,Blando JM,Allison JP,Xiong L,Subudhi SK,Gao J,Sharma P

Published figure using CD279 (PD-1) monoclonal antibody (Product # 11-9981-82) in Flow Cytometry

Mon Aug 08 00:00:00 EDT 2016

Negative CD4 + TIM-3 signaling confers resistance against cold preservation damage in mouse liver transplantation.

American journal of transplantation : official journal of the American Society of Transplantation and the American Society of Transplant Surgeons

Liu Y,Ji H,Zhang Y,Shen XD,Gao F,Nguyen TT,Shang X,Lee N,Busuttil RW,Kupiec-Weglinski JW

11-9981 was used in Flow cytometry/Cell sorting to explore TIM-3-Gal-9 function in a clinically relevant murine model of hepatic cold storage and orthotopic liver transplantation.

Wed Apr 01 00:00:00 EDT 2015

Costimulator B7-DC attenuates strong Th2 responses induced by Nippostrongylus brasiliensis.

Journal of immunology (Baltimore, Md. : 1950)

Ishiwata K,Watanabe N,Guo M,Tomihara K,Brumlik MJ,Yagita H,Pardoll D,Chen L,Shin T

11-9981 was used in Flow cytometry/Cell sorting to demonstrate that the inhibition of Th2 responses by B7-DC occurs independently of programmed death 1 but conceivably acts through an as yet unknown alternative receptor that enhances Th1 responses.

Mon Feb 15 00:00:00 EST 2010

Bioinformatics

Protein Aliases: CD279; hPD1; mPD-1; Programmed cell death protein 1; Protein PD-1; Protein PD1; sCD279; soluble CD279

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Gene Aliases: Ly101; PD-1; Pd1; Pdc1; Pdcd1

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UniProt ID: (Mouse) Q02242

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Entrez Gene ID: (Mouse) 18566

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Function(s)

protein binding

Process(es)

immune system process negative regulation of tolerance induction apoptotic process positive regulation of apoptotic process negative regulation of apoptotic process positive regulation of T cell apoptotic process

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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CD279 (PD-1) Monoclonal Antibody (RMP1-30), FITC, eBioscience™

CD279 (PD-1) Antibody (11-9981-82) in Flow

Product Details

11-9981-82

Flow Cytometry (Flow)

Species Reactivity

Published species

Host/Isotype

Recommended Isotype Control

Class

Type

Clone

Conjugate

Excitation/Emission Max

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

How to use the Panel Builder

Bioinformatics

Performance Guarantee

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