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Invitrogen
CD279 (PD-1) Monoclonal Antibody (RMP1-30), Super Bright™ 436, eBioscience™
FIGURE: 1 / 15
CD279 (PD-1) Antibody (62-9981-82) in Flow
BALB/c mouse splenocytes were unstimulated (left) or stimulated with CD3e and CD28 Monoclonal Antibodies, Functional Grade (Product # 16-0031-85 and Product # 16-0281-85) (right). Cells were then stained with 0.5 µg of Rat IgG2b kappa Isotype Control, Super Bright 436 (Product # 62-4031-82) (blue histogram) or CD279 Monoclonal Antibody, Super Bright 436 (purple histogram).Total viable cells were used for analysis, as determined by 7-AAD (Product # 00-6993-50).
Product Details
62-9981-82
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Mouse
Published species
Not Applicable
Host/Isotype
Rat
/ IgG2b, kappa
Recommended Isotype Control
Class
Monoclonal
Type
Antibody
Clone
RMP1-30
Conjugate
Excitation/Emission Max
413/431 nm
View spectra
Form
Liquid
Concentration
0.2 mg/mL
Purification
Affinity chromatography
Storage buffer
PBS, pH 7.2, with BSA
Contains
0.09% sodium azide
Storage conditions
4° C, store in dark, DO NOT FREEZE!
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2744824
Product Specific Information
Description: The RMP1-30 antibody reacts with mouse PD-1 (programmed death-1), a 55 kDa member of the Ig superfamily. PD-1 contains the immunoreceptor tyrosine-based inhibitory motif (ITIM) and plays a key role in peripheral tolerance and autoimmune disease in mice. PD-1 is expressed mainly on activated T and B lymphocytes. Two novel B7 Family members have been identified as PD-1 ligands, PD-L1 (B7-H1) and PD-L2 (B7-DC). Evidence reported to date suggests overlapping functions for these ligands and their constitutive expression on some normal tissues and upregulation on activated antigen-presenting cells. RMP1-30 does not block the binding of either B7-H1-Ig or B7-DC-Ig to PD-1 transfectants.
Applications Reported: This RMP1-30 antibody has been reported for use in flow cytometric analysis.
Applications Tested: This RMP1-30 antibody has been tested by flow cytometric analysis of activated mouse splenocytes. This may be used at less than or equal to 1 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.
Super Bright 436 can be excited with the violet laser line (405 nm) and emits at 436 nm. We recommend using a 450/50 bandpass filter, or equivalent. Please make sure that your instrument is capable of detecting this fluorochrome.
When using two or more Super Bright dye-conjugated antibodies in a staining panel, it is recommended to use Super Bright Complete Staining Buffer (Product # SB-4401) to minimize any non-specific polymer interactions. Please refer to the datasheet for Super Bright Staining Buffer for more information.
Excitation: 405 nm; Emission: 436 nm; Laser: Violet Laser
Super Bright Polymer Dyes are sold under license from Becton, Dickinson and Company.
Target Information
Cell-mediated immune responses are initiated by T lymphocytes that are themselves stimulated by cognate peptides bound to MHC molecules on antig en-presenting cells (APC). T-cell activation is generally self-limited as activated T cells express receptors such as PD-1 (also known as PDCD-1) that mediate inhibitory signals from the APC. PD-1 can bind two different but related ligands, PDL-1 and PDL-2. Upon binding to either of these ligands, signals generated by PD-1 inhibit the activation of the immune response in the absence of "danger signals" such as LPS or other molecules associated with bacteria or other pathogens. Evidence for this is seen in PD1-null mice who exhibit hyperactivated immune systems and autoimmune diseases. Despite its predicted molecular weight, PD-1 often migrates at higher molecular weight in SDS-PAGE.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
How to use the Panel Builder
Watch the video to learn how to use the Invitrogen Flow Cytometry Panel Builder to build your next flow cytometry panel in 5 easy steps.
Bioinformatics
Protein Aliases: CD279; hPD1; mPD-1; Programmed cell death protein 1; Protein PD-1; Protein PD1; sCD279; soluble CD279
Gene Aliases: Ly101; PD-1; Pd1; Pdc1; Pdcd1
UniProt ID: (Mouse) Q02242
Entrez Gene ID: (Mouse) 18566
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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