Invitrogen

CD16 Monoclonal Antibody (eBioCB16 (CB16)), PE, eBioscience™

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This Antibody was verified by Relative expression to ensure that the antibody binds to the antigen stated.

23 Published Figures

30 References

View all (168) CD16 antibodies

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CD16 Antibody (12-0168-42) in Flow

Staining of normal human peripheral blood cells with Anti-Human CD56 (NCAM) FITC (Product # 11-0569) and Mouse IgG1 K Isotype Control PE (Product # 12-4714-81) (left) or Anti-Human CD16 PE (right). Cells in the lymphocyte gate were used for analysis.

Product Image Gallery

CD16 Antibody — Staining of human peripheral blood cells. As expected based on known relative expression patterns, CD16 clone CB16 stains all granulocytes, a subset of monocytes and a subset of lymphocytes (NK cells). Details: Normal human whole blood was surface stained with CD16 (clone CB16). After staining, red blood cells were lysed using 1-step Fix/Lyse Buffer. Cells in the lymphocyte (purple histogram), monocyte (orange histogram), or granulocyte (blue histogram) gates were used for analysis. {RE}

Product Details

12-0168-42

Applications

Tested Dilution

Publications

Flow Cytometry (Flow)

5 µL (0.06 µg)/test

View 30 publications 30 publications

Product Specifications

Species Reactivity

Human

Published species

Human

Host/Isotype

Mouse / IgG1, kappa

Recommended Isotype Control

Mouse IgG1 kappa Isotype Control (P3.6.2.8.1), PE, eBioscience™

Class

Monoclonal

Type

Antibody

Clone

eBioCB16 (CB16)

Conjugate

PE PE PE

Excitation/Emission Max

565/576 nm View spectra

Form

Liquid

Concentration

5 µL/Test

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.2, with 0.2% BSA

Contains

0.09% sodium azide

Storage conditions

4° C, store in dark, DO NOT FREEZE!

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_11043421

Product Specific Information

Description: The eBioCB16 monoclonal antibody recognizes CD16 (Fc gammaRIII), the low-affinity receptor for IgG with an apparent molecular weight of 50-80 kDa. CD16 is represented by two similar genes, CD16A (Fc gammaRIIIA), which exists as a hetero-oligomeric polypeptide-anchored form in macrophages and NK cells and CD16B (Fc gammaRIIIB), which exist as a monomeric GPI-anchored form in neutrophils. Furthermore, there are two known polymorphisms of CD16B, NA-1 and NA-2. Individuals homozygous for NA-2 show a lower phagocytic capacity compared with NA-1. CD16 binds IgG in the form of immune complexes and shows preferential binding of IgG1 and IgG3 isotypes and minimal binding of IgG2 and IgG4. Upon IgG binding, both CD16 isoforms initiate signal transduction cascades that lead to a variety of responses including antibody-dependent cell-mediated cytotoxicity (ADCC), phagocytosis, degranulation and proliferation.

Applications Reported: This eBioCB16 (CB16) antibody has been reported for use in flow cytometric analysis.

Applications Tested: This eBioCB16 (CB16) antibody has been pre-titrated and tested by flow cytometric analysis of normal normal human peripheral blood cells. This can be used at 5 µL (0.06 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test.

Excitation: 488-561 nm; Emission: 578 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser.

Filtration: 0.2 µm post-manufacturing filtered.

Target Information

CD16 encodes a receptor that recognizes the Fc portion of immunoglobulin G and is involved in the clearance of immune complexes from the circulation, as well as other functions such as cellular mediated cytotoxicity and enhancement of virus infections. This gene, FCGR3A, shares a high degree of similarity with another nearby gene, FCGR3B, located on chromosome 1. The receptor encoded by this gene is expressed on natural killer (NK) cells as an integral membrane glycoprotein anchored through a transmembrane peptide, while FCGR3B is expressed on polymorphonuclear neutrophils (PMN) where the receptor is anchored through a phosphatidylinositol (PI) linkage. Mutations in this gene have been associated with immunodeficiency 20 and have been linked to susceptibility to recurrent viral infections, susceptibility to systemic lupus erythematosus, and alloimmune neonatal neutropenia. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. Diseases associated with FCGR3A include Immunodeficiency 20 and Herpes Zoster.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Product References

SARS-CoV-2 Delta (B.1.617.2) variant replicates and induces syncytia formation in human induced pluripotent stem cell-derived macrophages.

PeerJ

Thaweerattanasinp T,Wanitchang A,Saenboonrueng J,Srisutthisamphan K,Wanasen N,Sungsuwan S,Jongkaewwattana A,Chailangkarn T

Published figure using CD16 monoclonal antibody (Product # 12-0168-42) in Flow Cytometry

Thu Mar 09 00:00:00 EST 2023

Prognostic Lnc-S100B-2 Affects Cell Apoptosis and Microenvironment of Colorectal Cancer through MLLT10 Signaling.

Journal of oncology

Yi J,Peng F,Zhao J,Gong X

Published figure using CD16 monoclonal antibody (Product # 12-0168-42) in Flow Cytometry

Tue Feb 08 00:00:00 EST 2022

Gene-dosage effect of Pfkfb3 on monocyte/macrophage biology in atherosclerosis.

British journal of pharmacology

Guo S,Li A,Fu X,Li Z,Cao K,Song M,Huang S,Li Z,Yan J,Wang L,Dai X,Feng D,Wang Y,He J,Huo Y,Xu Y

Published figure using CD16 monoclonal antibody (Product # 12-0168-42) in Flow Cytometry

Tue Nov 01 00:00:00 EDT 2022

CD39/CD73 Dysregulation of Adenosine Metabolism Increases Decidual Natural Killer Cell Cytotoxicity: Implications in Unexplained Recurrent Spontaneous Abortion.

Frontiers in immunology

Zhu J,Song G,Zhou X,Han TL,Yu X,Chen H,Mansell T,Novakovic B,Baker PN,Cannon RD,Saffery R,Chen C,Zhang H

12-0168-42 was used in Flow cytometry/Cell sorting to investigate the regulation of decidual natural killer (dNK) cells and fetal extravillous trophoblast (EVT) cells by CD39 and CD73 in URSA, as well as the possible regulatory mechanism of CD39/CD73 via the TGF-β-mTOR-HIF-1α pathway using clinical samples and cell models.

Fri Apr 08 00:00:00 EDT 2022

Endothelial dysfunction markers and immune response indices in cosmonauts' blood after long-duration space flights.

NPJ microgravity

Kuzichkin DS,Nichiporuk IA,Zhuravleva OA,Markin AA,Rykova MP,Zhuravleva TV,Sadova AA,Kutko OV,Shmarov VA,Ponomarev SA

12-0168-42 was used in Flow cytometry/Cell sorting to investigat the markers of endothelial activation and damage (plasma concentrations of soluble thrombomodulin fraction (sTM), von Willebrand factor (vWF), highly sensitive C-reactive protein (hs-CRP)), as well as the level of D-dimer and compared them to the immunological parameters characterizing the state of human humoral and cellular immunity.

Wed Nov 02 00:00:00 EDT 2022

Broad and potent neutralizing human antibodies to tick-borne flaviviruses protect mice from disease.

The Journal of experimental medicine

Agudelo M,Palus M,Keeffe JR,Bianchini F,Svoboda P,Salát J,Peace A,Gazumyan A,Cipolla M,Kapoor T,Guidetti F,Yao KH,Elsterová J,Teislerová D,Chrdle A,Hönig V,Oliveira T,West AP,Lee YE,Rice CM,MacDonald MR,Bjorkman PJ,Růžek D,Robbiani DF,Nussenzweig MC

Published figure using CD16 monoclonal antibody (Product # 12-0168-42) in Flow Cytometry

Mon May 03 00:00:00 EDT 2021

Heterogeneous disease-propagating stem cells in juvenile myelomonocytic leukemia.

The Journal of experimental medicine

Louka E,Povinelli B,Rodriguez-Meira A,Buck G,Wen WX,Wang G,Sousos N,Ashley N,Hamblin A,Booth CAG,Roy A,Elliott N,Iskander D,de la Fuente J,Fordham N,O'Byrne S,Inglott S,Norfo R,Salio M,Thongjuea S,Rao A,Roberts I,Mead AJ

Published figure using CD16 monoclonal antibody (Product # 12-0168-42) in Flow Cytometry

Mon Feb 01 00:00:00 EST 2021

A peptide derived from chaperonin 60.1, IRL201104, inhibits LPS-induced acute lung inflammation.

American journal of physiology. Lung cellular and molecular physiology

Man F,Nadkarni S,Kanabar V,E-Lacerda RR,Gomes Ferreira S,Federici Canova D,Perretti M,Page CP,Riffo-Vasquez Y

12-0168-42 was used in Flow Cytometry to investigate the anti-inflammatory properties of chaperonin 60.1 (Cpn60.1) and its peptide fragment IRL201104 in a model of nonallergic lung inflammation.

Mon Nov 01 00:00:00 EDT 2021

Isolation of polymorphonuclear neutrophils and monocytes from a single sample of human peripheral blood.

STAR protocols

Cui C,Schoenfelt KQ,Becker KM,Becker L

12-0168-42 was used in Flow Cytometry to present a detailed protocol to isolate polymorphonuclear neutrophils and monocytes from a single sample of human peripheral blood.

Fri Dec 17 00:00:00 EST 2021

Isolation of polymorphonuclear neutrophils and monocytes from a single sample of human peripheral blood.

STAR protocols

Cui C,Schoenfelt KQ,Becker KM,Becker L

12-0168-42 was used in Flow Cytometry to present a detailed protocol to isolate polymorphonuclear neutrophils and monocytes from a single sample of human peripheral blood.

Fri Dec 17 00:00:00 EST 2021

-------A type I IFN, prothrombotic hyperinflammatory neutrophil signature is distinct for COVID-19 ARDS--.

Wellcome open research

Reyes L,A Sanchez-Garcia M,Morrison T,Howden AJM,Watts ER,Arienti S,Sadiku P,Coelho P,Mirchandani AS,Zhang A,Hope D,Clark SK,Singleton J,Johnston S,Grecian R,Poon A,McNamara S,Harper I,Fourman MH,Brenes AJ,Pathak S,Lloyd A,Blanco GR,von Kriegsheim A,Ghesquiere B,Vermaelen W,Cologna CT,Dhaliwal K,Hirani N,Dockrell DH,Whyte MKB,Griffith D,Cantrell DA,Walmsley SR

Published figure using CD16 monoclonal antibody (Product # 12-0168-42) in Flow Cytometry

Wed Feb 15 00:00:00 EST 2023

Associations between Hypertriglyceridemia and Circulating Neutrophil Subpopulation in Patients with Dyslipidemia.

International journal of inflammation

Genkel V,Dolgushin I,Baturina I,Savochkina A,Kuznetsova A,Pykhova L,Shaposhnik I

Published figure using CD16 monoclonal antibody (Product # 12-0168-42) in Flow Cytometry

Sun Apr 24 00:00:00 EDT 2022

Diurnal Variation of Plasma Extracellular Vesicle Is Disrupted in People Living with HIV.

Pathogens (Basel, Switzerland)

Bazié WW,Goyer B,Boucher J,Zhang Y,Planas D,Chatterjee D,Routy JP,Alary M,Ancuta P,Gilbert C

12-0168-42 was used in Flow Cytometry to investigate daily variations of large and small EVs, in terms of abundance and microRNA contents in people living with HIV (PLWH) receiving antiretroviral therapy (HIV+ART) and uninfected controls (HIV-).

Sat Apr 24 00:00:00 EDT 2021

SARS-CoV-2-triggered neutrophil extracellular traps mediate COVID-19 pathology.

The Journal of experimental medicine

Veras FP,Pontelli MC,Silva CM,Toller-Kawahisa JE,de Lima M,Nascimento DC,Schneider AH,Caetité D,Tavares LA,Paiva IM,Rosales R,Colón D,Martins R,Castro IA,Almeida GM,Lopes MIF,Benatti MN,Bonjorno LP,Giannini MC,Luppino-Assad R,Almeida SL,Vilar F,Santana R,Bollela VR,Auxiliadora-Martins M,Borges M,Miranda CH,Pazin-Filho A,da Silva LLP,Cunha LD,Zamboni DS,Dal-Pizzol F,Leiria LO,Siyuan L,Batah S,Fabro A,Mauad T,Dolhnikoff M,Duarte-Neto A,Saldiva P,Cunha TM,Alves-Filho JC,Arruda E,Louzada-Junior P,Oliveira RD,Cunha FQ

12-0168 was used in Flow cytometry/Cell sorting to result unravel a possible detrimental role of NETs in the pathophysiology of COVID-19. Therefore, the inhibition of NETs represents a potential therapeutic target for COVID-19.

Mon Dec 07 00:00:00 EST 2020

SARS-CoV-2-triggered neutrophil extracellular traps mediate COVID-19 pathology.

The Journal of experimental medicine

Mon Dec 07 00:00:00 EST 2020

IL-36 Promotes Systemic IFN-I Responses in Severe Forms of Psoriasis.

The Journal of investigative dermatology

Catapano M,Vergnano M,Romano M,Mahil SK,Choon SE,Burden AD,Young HS,Carr IM,Lachmann HJ,Lombardi G,Smith CH,Ciccarelli FD,Barker JN,Capon F

Published figure using CD16 monoclonal antibody (Product # 12-0168-42) in Flow Cytometry

Wed Apr 01 00:00:00 EDT 2020

CD14⁺CD16^- monocytes are the main precursors of osteoclasts in rheumatoid arthritis via expressing Tyro3TK.

Arthritis research & therapy

Xue J,Xu L,Zhu H,Bai M,Li X,Zhao Z,Zhong H,Cheng G,Li X,Hu F,Su Y

Published figure using CD16 monoclonal antibody (Product # 12-0168-42) in Flow Cytometry

Mon Sep 21 00:00:00 EDT 2020

Cutting edge approaches for rapid characterization of airway exosomes.

Methods (San Diego, Calif.)

Hough KP,Deshane JS

12-0168 was used in Flow cytometry/Cell sorting to describe two methods of rapid exosomes characterization: (1) imaging flow cytometry using ImageStream; and (2) conventional flow cytometry using the BD Symphony A5 platform.

Fri May 01 00:00:00 EDT 2020

Circulating Neutrophils of Nonalcoholic Steatohepatitis Patients Show an Activated Phenotype and Suppress T Lymphocytes Activity.

Journal of immunology research

Antonucci L,Porcu C,Timperi E,Santini SJ,Iannucci G,Balsano C

Published figure using CD16 monoclonal antibody (Product # 12-0168-42) in Flow Cytometry

Fri May 21 00:00:00 EDT 2021

Loss of testosterone impairs anti-tumor neutrophil function.

Nature communications

Markman JL,Porritt RA,Wakita D,Lane ME,Martinon D,Noval Rivas M,Luu M,Posadas EM,Crother TR,Arditi M

Published figure using CD16 monoclonal antibody (Product # 12-0168-42) in Flow Cytometry

Tue Mar 31 00:00:00 EDT 2020

A FACS-based approach to obtain viable eosinophils from human adipose tissue.

Scientific reports

Hernandez JD,Tew BY,Li T,Gooden GC,Ghannam H,Masuda M,Madura J,Salhia B,Jacobsen EA,De Filippis E

Published figure using CD16 monoclonal antibody (Product # 12-0168-42) in Flow Cytometry

Thu Aug 06 00:00:00 EDT 2020

Infection of human lymphomononuclear cells by SARS-CoV-2.

bioRxiv : the preprint server for biology

Pontelli MC,Castro IA,Martins RB,Veras FP,Serra L,Nascimento DC,Cardoso RS,Rosales R,Lima TM,Souza JP,Caetité DB,de Lima MHF,Kawahisa JT,Giannini MC,Bonjorno LP,Lopes MIF,Batah SS,Siyuan L,Assad RL,Almeida SCL,Oliveira FR,Benatti MN,Pontes LLF,Santana RC,Vilar FC,Martins MA,Cunha TM,Calado RT,Alves-Filho JC,Zamboni DS,Fabro A,Louzada-Junior P,Oliveira RDR,Cunha FQ,Arruda E

12-0168-42 was used in Flow Cytometry to show that SARS-CoV-2 infection of blood circulating leukocytes in COVID-19 patients may have important implications for disease pathogenesis, immune dysfunction, and virus spread within the host.

Fri Aug 07 00:00:00 EDT 2020

Identification of an activated neutrophil phenotype in polymyalgia rheumatica during steroid treatment: a potential involvement of immune cell cross-talk.

Clinical science (London, England : 1979)

Nadkarni S,Lashin H,Hollywood J,Dasgupta B,Mason JC,Perretti M

12-0168 was used in Flow cytometry/Cell sorting to indicate a clear distinction from GCA patient neutrophil and MV signatures, and provide an opportunity for further investigations on how to 'stratify' PMR patients and monitor their clinical responses through novel use of blood biomarkers.

Mon Apr 15 00:00:00 EDT 2019

Bacillus anthracis lethal toxin negatively modulates ILC3 function through perturbation of IL-23-mediated MAPK signaling.

PLoS pathogens

Seshadri S,Allan DSJ,Carlyle JR,Zenewicz LA

12-0168 was used in Flow cytometry/Cell sorting to study the effects of MAPK signalling pathways in group 3 innate immune lymphocytes using Bacillus anthracis.

Sun Oct 01 00:00:00 EDT 2017

Ex vivo culture of human atherosclerotic plaques: A model to study immune cells in atherogenesis.

Atherosclerosis

Lebedeva A,Vorobyeva D,Vagida M,Ivanova O,Felker E,Fitzgerald W,Danilova N,Gontarenko V,Shpektor A,Vasilieva E,Margolis L

Published figure using CD16 monoclonal antibody (Product # 12-0168-42) in Flow Cytometry

Fri Dec 01 00:00:00 EST 2017

Metformin-treated cancer cells modulate macrophage polarization through AMPK-NF-κB signaling.

Oncotarget

Chiang CF,Chao TT,Su YF,Hsu CC,Chien CY,Chiu KC,Shiah SG,Lee CH,Liu SY,Shieh YS

Published figure using CD16 monoclonal antibody (Product # 12-0168-42) in Flow Cytometry

Tue Mar 28 00:00:00 EDT 2017

Pulmonary sarcoidosis is associated with high-level inducible co-stimulator (ICOS) expression on lung regulatory T cells--possible implications for the ICOS/ICOS-ligand axis in disease course and resolution.

Clinical and experimental immunology

Sakthivel P,Grunewald J,Eklund A,Bruder D,Wahlström J

Published figure using CD16 monoclonal antibody (Product # 12-0168-42) in Flow Cytometry

Mon Feb 01 00:00:00 EST 2016

TNFα promotes CAR-dependent migration of leukocytes across epithelial monolayers.

Scientific reports

Morton PE,Hicks A,Ortiz-Zapater E,Raghavan S,Pike R,Noble A,Woodfin A,Jenkins G,Rayner E,Santis G,Parsons M

12-0168 was used in Flow cytometry/Cell sorting to demonstrate that trans-epithelial migration of monocyte-derived THP-1 cells is regulated by phosphorylation of the Coxsackie and Adenovirus receptor.

Thu May 19 00:00:00 EDT 2016

Involvement of Macrophages in the Pathogenesis of Familial Amyloid Polyneuropathy and Efficacy of Human iPS Cell-Derived Macrophages in Its Treatment.

PloS one

Suenaga G,Ikeda T,Komohara Y,Takamatsu K,Kakuma T,Tasaki M,Misumi Y,Ueda M,Ito T,Senju S,Ando Y

12-0168 was used in Flow cytometry/Cell sorting to evaluate abnormalities in heart tissue-resident macrophages in familial amyloid polyneuropathy.

Thu Jun 15 00:00:00 EDT 2017

Retinoic acid regulates hematopoietic development from human pluripotent stem cells.

Stem cell reports

Rönn RE,Guibentif C,Moraghebi R,Chaves P,Saxena S,Garcia B,Woods NB

Published figure using CD16 monoclonal antibody (Product # 12-0168-42) in Flow Cytometry

Tue Feb 10 00:00:00 EST 2015

The potential role of CD16+ Vγ2Vδ2 T cell-mediated antibody-dependent cell-mediated cytotoxicity in control of HIV type 1 disease.

AIDS research and human retroviruses

He X,Liang H,Hong K,Li H,Peng H,Zhao Y,Jia M,Ruan Y,Shao Y

12-0168 was used in Flow cytometry/Cell sorting to characterise the two distinct and complementary Vδ2 T cell subsets and explore the respective impacts of HIV-1 on them.

Sun Dec 01 00:00:00 EST 2013

Changes in natural killer cell subsets in pediatric liver transplant recipients.

Pediatric transplantation

Pham B,Piard-Ruster K,Silva R,Gallo A,Esquivel CO,Martinez OM,Krams SM

Published figure using CD16 monoclonal antibody (Product # 12-0168-42) in Flow Cytometry

Thu Mar 01 00:00:00 EST 2012

Bioinformatics

Protein Aliases: CD16-I; CD16-II; CD16a; CD16a antigen; CD16b; CD32 receptor 2; Fc fragment of IgG low affinity IIIa receptor; Fc fragment of IgG, low affinity III, receptor for (CD16); Fc fragment of IgG, low affinity IIIa, receptor (CD16a); Fc fragment of IgG, low affinity IIIb, receptor (CD16b); Fc gamma receptor III-A; Fc gamma receptor IIIa; Fc gamma receptor IIIb; Fc-gamma receptor III-2 (CD 16); Fc-gamma receptor IIIb (CD 16); Fc-gamma receptor IIIb (CD16); Fc-gamma RIII; Fc-gamma RIII-alpha; Fc-gamma RIII-beta; Fc-gamma RIIIa; fc-gamma RIIIb; FCG2; FcgammaRIIIA; FCGR2; FCGR2A; FCGR2B; FCGR2C; FcR-10; FcRII-b; FcRII-c; IgG Fc receptor III-1; IgG Fc receptor III-2; IgG Fc receptor III-A; immunoglobulin G Fc receptor III; Low affinity immunoglobulin gamma Fc region receptor III-A; Low affinity immunoglobulin gamma Fc region receptor III-B; neutrophil-specific antigen NA; RP11-5K23.1

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Gene Aliases: CD16; CD16A; CD16B; FCG3; FCGR3; FCGR3A; FCGR3B; FCGRIII; FCR-10; FCRIII; FCRIIIA; FCRIIIb; IGFR3; IMD20

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UniProt ID: (Human) P08637, (Human) O75015

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Entrez Gene ID: (Human) 2214, (Human) 2215

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Function(s)

IgG binding immunoglobulin receptor superfamily

Process(es)

immune response Fc-gamma receptor signaling pathway involved in phagocytosis regulation of immune response positive regulation of defense response to virus by host mitophagy in response to mitochondrial depolarization xenophagy

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CD16 Monoclonal Antibody (eBioCB16 (CB16)), PE, eBioscience™

CD16 Antibody (12-0168-42) in Flow

Product Details

12-0168-42

Flow Cytometry (Flow)

Species Reactivity

Published species

Host/Isotype

Recommended Isotype Control

Class

Type

Clone

Conjugate

Excitation/Emission Max

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

How to use the Panel Builder

Bioinformatics

Performance Guarantee

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