Invitrogen

CD16 Monoclonal Antibody (eBioCB16 (CB16)), APC-eFluor™ 780, eBioscience™

Advanced Verification

This Antibody was verified by Relative expression to ensure that the antibody binds to the antigen stated.

23 Published Figures

41 References

View all (168) CD16 antibodies

Datasheet

Protocols

Questions & Answers

Tech Support

Datasheet

Protocols

Questions & Answers

Tech Support

FIGURE: 1 / 25

CD16 Antibody (47-0168-42) in Flow

Staining of normal human peripheral blood cells with Anti-Human CD56 (NCAM) FITC (Product # 11-0569) and Mouse IgG1 K Isotype Control APC-eFluor® 780 (Product # 47-4714-82) (left) or Anti-Human CD16 APC-eFluor® 780 (right). Cells in the lymphocyte gate were used for analysis.

Product Image Gallery

CD16 Antibody — Staining of human peripheral blood cells. As expected based on known relative expression patterns, CD16 clone CB16 stains all granulocytes, a subset of monocytes and a subset of lymphocytes (NK cells). Details: Normal human whole blood was surface stained with CD16 (clone CB16). After staining, red blood cells were lysed using 1-step Fix/Lyse Buffer. Cells in the lymphocyte (purple histogram), monocyte (orange histogram), or granulocyte (blue histogram) gates were used for analysis. {RE}

Product Details

47-0168-42

Applications

Tested Dilution

Publications

Flow Cytometry (Flow)

5 µL (0.25 µg)/test

View 41 publications 41 publications

Product Specifications

Species Reactivity

Human

Published species

Human, Mouse, Non-human primate, Rhesus monkey

Host/Isotype

Mouse / IgG1, kappa

Recommended Isotype Control

Mouse IgG1 kappa Isotype Control (P3.6.2.8.1), APC-eFluor™ 780, eBioscience™

Class

Monoclonal

Type

Antibody

Clone

eBioCB16 (CB16)

Conjugate

APC-eFluor™ 780 APC-eFluor™ 780 APC-eFluor™ 780

Excitation/Emission Max

756/785 nm View spectra

Form

Liquid

Concentration

5 µL/Test

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.2, with 0.2% BSA

Contains

0.09% sodium azide

Storage conditions

4° C, store in dark, DO NOT FREEZE!

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_11220086

Product Specific Information

Description: The eBioCB16 monoclonal antibody recognizes CD16 (Fc gammaRIII), the low-affinity receptor for IgG with an apparent molecular weight of 50-80 kDa. CD16 is represented by two similar genes, CD16A (Fc gammaRIIIA), which exists as a hetero-oligomeric polypeptide-anchored form in macrophages and NK cells and CD16B (Fc gammaRIIIB), which exist as a monomeric GPI-anchored form in neutrophils. Furthermore, there are two known polymorphisms of CD16B, NA-1 and NA-2. Individuals homozygous for NA-2 show a lower phagocytic capacity compared with NA-1. CD16 binds IgG in the form of immune complexes and shows preferential binding of IgG1 and IgG3 isotypes and minimal binding of IgG2 and IgG4. Upon IgG binding, both CD16 isoforms initiate signal transduction cascades that lead to a variety of responses including antibody-dependent cell-mediated cytotoxicity (ADCC), phagocytosis, degranulation and proliferation.

Applications Reported: This CB16 antibody has been reported for use in flow cytometric analysis.

Applications Tested: This eBioCB16 (CB16) antibody has been pre-titrated and tested by flow cytometric analysis of normal human peripheral blood cells. This can be used at 5 µL (0.25 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test.

APC-eFluor 780 emits at 780 nm and is excited with the Red laser (633 nm). Please make sure that your instrument is capable of detecting this fluorochome.

Light sensitivity: This tandem is sensitive to photo-induced oxidation. Please protect this vial and stained samples from light.

Fixation: Samples can be stored in IC Fixation Buffer (Product # 00-8222) (100 µL cell sample + 100 µL IC Fixation Buffer) or 1-step Fix/Lyse Solution (Product # 00-5333) for up to 3 days in the dark at 4°C with minimal impact on brightness and FRET efficiency/compensation. Some generalizations regarding fluorophore performance after fixation can be made, but clone specific performance should be determined empirically.

Excitation: 633-647 nm; Emission: 780 nm; Laser: Red Laser.

Filtration: 0.2 µm post-manufacturing filtered.

Target Information

CD16 encodes a receptor that recognizes the Fc portion of immunoglobulin G and is involved in the clearance of immune complexes from the circulation, as well as other functions such as cellular mediated cytotoxicity and enhancement of virus infections. This gene, FCGR3A, shares a high degree of similarity with another nearby gene, FCGR3B, located on chromosome 1. The receptor encoded by this gene is expressed on natural killer (NK) cells as an integral membrane glycoprotein anchored through a transmembrane peptide, while FCGR3B is expressed on polymorphonuclear neutrophils (PMN) where the receptor is anchored through a phosphatidylinositol (PI) linkage. Mutations in this gene have been associated with immunodeficiency 20 and have been linked to susceptibility to recurrent viral infections, susceptibility to systemic lupus erythematosus, and alloimmune neonatal neutropenia. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. Diseases associated with FCGR3A include Immunodeficiency 20 and Herpes Zoster.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

How to use the Panel Builder

Watch the video to learn how to use the Invitrogen Flow Cytometry Panel Builder to build your next flow cytometry panel in 5 easy steps.

Product References

Antibody feedback regulates immune memory after SARS-CoV-2 mRNA vaccination.

Nature

Schaefer-Babajew D,Wang Z,Muecksch F,Cho A,Loewe M,Cipolla M,Raspe R,Johnson B,Canis M,DaSilva J,Ramos V,Turroja M,Millard KG,Schmidt F,Witte L,Dizon J,Shimeliovich I,Yao KH,Oliveira TY,Gazumyan A,Gaebler C,Bieniasz PD,Hatziioannou T,Caskey M,Nussenzweig MC

47-0168-42 was used in Flow cytometry/Cell sorting to indicate that pre-existing high-affinity antibodies bias germinal centre and memory B cell selection through two distinct mechanisms: (1) by lowering the activation threshold for B cells, thereby permitting abundant lower-affinity clones to participate in the immune response; and (2) through direct masking of their cognate epitopes.

Sun Jan 01 00:00:00 EST 2023

SARS-CoV-2 Delta (B.1.617.2) variant replicates and induces syncytia formation in human induced pluripotent stem cell-derived macrophages.

PeerJ

Thaweerattanasinp T,Wanitchang A,Saenboonrueng J,Srisutthisamphan K,Wanasen N,Sungsuwan S,Jongkaewwattana A,Chailangkarn T

Published figure using CD16 monoclonal antibody (Product # 47-0168-42) in Flow Cytometry

Thu Mar 09 00:00:00 EST 2023

Prognostic Lnc-S100B-2 Affects Cell Apoptosis and Microenvironment of Colorectal Cancer through MLLT10 Signaling.

Journal of oncology

Yi J,Peng F,Zhao J,Gong X

Published figure using CD16 monoclonal antibody (Product # 47-0168-42) in Flow Cytometry

Tue Feb 08 00:00:00 EST 2022

Gene-dosage effect of Pfkfb3 on monocyte/macrophage biology in atherosclerosis.

British journal of pharmacology

Guo S,Li A,Fu X,Li Z,Cao K,Song M,Huang S,Li Z,Yan J,Wang L,Dai X,Feng D,Wang Y,He J,Huo Y,Xu Y

Published figure using CD16 monoclonal antibody (Product # 47-0168-42) in Flow Cytometry

Tue Nov 01 00:00:00 EDT 2022

The memory B cell response to influenza vaccination is impaired in older persons.

Cell reports

Burton AR,Guillaume SM,Foster WS,Wheatley AK,Hill DL,Carr EJ,Linterman MA

47-0168-42 was used in Flow cytometry/Cell sorting to show that the aged human GC reaction and memory B cell response following vaccination is defective.

Tue Nov 08 00:00:00 EST 2022

Boosting corrects a memory B cell defect in SARS-CoV-2 mRNA-vaccinated patients with inflammatory bowel disease.

JCI insight

Pape KA,Dileepan T,Matchett WE,Ellwood C,Stresemann S,Kabage AJ,Kozysa D,Evert C,Matson M,Lopez S,Krueger PD,Graiziger CT,Vaughn BP,Shmidt E,Rhein J,Schacker TW,Bold TD,Langlois RA,Khoruts A,Jenkins MK

47-0168-42 was used in Flow cytometry/Cell sorting to show that patients with IBD have defects in the formation of antibody-secreting B cells and affinity-matured memory B cells that are corrected by a third vaccination.

Wed Jun 22 00:00:00 EDT 2022

Inactivated whole-virion vaccine BBV152/Covaxin elicits robust cellular immune memory to SARS-CoV-2 and variants of concern.

Nature microbiology

Vikkurthi R,Ansari A,Pai AR,Jha SN,Sachan S,Pandit S,Nikam B,Kalia A,Jit BP,Parray HA,Singh S,Kshetrapal P,Wadhwa N,Shrivastava T,Coshic P,Kumar S,Sharma P,Sharma N,Taneja J,Pandey AK,Sharma A,Thiruvengadam R,Grifoni A,Weiskopf D,Sette A,Bhatnagar S,Gupta N

47-0168-42 was used in Flow cytometry/Cell sorting to show that inactivated vaccine BBV152 induces robust immune memory to SARS-CoV-2 and variants of concern that persists for at least 6 months after vaccination.

Fri Jul 01 00:00:00 EDT 2022

mRNA vaccine-elicited antibodies to SARS-CoV-2 and circulating variants.

Nature

Wang Z,Schmidt F,Weisblum Y,Muecksch F,Barnes CO,Finkin S,Schaefer-Babajew D,Cipolla M,Gaebler C,Lieberman JA,Oliveira TY,Yang Z,Abernathy ME,Huey-Tubman KE,Hurley A,Turroja M,West KA,Gordon K,Millard KG,Ramos V,Da Silva J,Xu J,Colbert RA,Patel R,Dizon J,Unson-O'Brien C,Shimeliovich I,Gazumyan A,Caskey M,Bjorkman PJ,Casellas R,Hatziioannou T,Bieniasz PD,Nussenzweig MC

47-0168-42 was used in Flow Cytometry to report on the antibody and memory B cell responses of a cohort of 20 volunteers who received the Moderna (mRNA-1273) or Pfizer-BioNTech (BNT162b2) vaccine against SARS-CoV-21-4.

Thu Apr 01 00:00:00 EDT 2021

Broad and potent neutralizing human antibodies to tick-borne flaviviruses protect mice from disease.

The Journal of experimental medicine

Agudelo M,Palus M,Keeffe JR,Bianchini F,Svoboda P,Salát J,Peace A,Gazumyan A,Cipolla M,Kapoor T,Guidetti F,Yao KH,Elsterová J,Teislerová D,Chrdle A,Hönig V,Oliveira T,West AP,Lee YE,Rice CM,MacDonald MR,Bjorkman PJ,Růžek D,Robbiani DF,Nussenzweig MC

47-0168-42 was used in Flow Cytometry to report on the human neutralizing antibody response to TBEV in a cohort of infected and vaccinated individuals.

Mon May 03 00:00:00 EDT 2021

Broad and potent neutralizing human antibodies to tick-borne flaviviruses protect mice from disease.

The Journal of experimental medicine

47-0168-42 was used in Flow Cytometry to report on the human neutralizing antibody response to TBEV in a cohort of infected and vaccinated individuals.

Mon May 03 00:00:00 EDT 2021

Heterogeneous disease-propagating stem cells in juvenile myelomonocytic leukemia.

The Journal of experimental medicine

Louka E,Povinelli B,Rodriguez-Meira A,Buck G,Wen WX,Wang G,Sousos N,Ashley N,Hamblin A,Booth CAG,Roy A,Elliott N,Iskander D,de la Fuente J,Fordham N,O'Byrne S,Inglott S,Norfo R,Salio M,Thongjuea S,Rao A,Roberts I,Mead AJ

Published figure using CD16 monoclonal antibody (Product # 47-0168-42) in Flow Cytometry

Mon Feb 01 00:00:00 EST 2021

Guidelines for the use of flow cytometry and cell sorting in immunological studies (third edition).

European journal of immunology

Cossarizza A,Chang HD,Radbruch A,Abrignani S,Addo R,Akdis M,Andrä I,Andreata F,Annunziato F,Arranz E,Bacher P,Bari S,Barnaba V,Barros-Martins J,Baumjohann D,Beccaria CG,Bernardo D,Boardman DA,Borger J,Böttcher C,Brockmann L,Burns M,Busch DH,Cameron G,Cammarata I,Cassotta A,Chang Y,Chirdo FG,Christakou E,Čičin-Šain L,Cook L,Corbett AJ,Cornelis R,Cosmi L,Davey MS,De Biasi S,De Simone G,Del Zotto G,Delacher M,Di Rosa F,Di Santo J,Diefenbach A,Dong J,Dörner T,Dress RJ,Dutertre CA,Eckle SBG,Eede P,Evrard M,Falk CS,Feuerer M,Fillatreau S,Fiz-Lopez A,Follo M,Foulds GA,Fröbel J,Gagliani N,Galletti G,Gangaev A,Garbi N,Garrote JA,Geginat J,Gherardin NA,Gibellini L,Ginhoux F,Godfrey DI,Gruarin P,Haftmann C,Hansmann L,Harpur CM,Hayday AC,Heine G,Hernández DC,Herrmann M,Hoelsken O,Huang Q,Huber S,Huber JE,Huehn J,Hundemer M,Hwang WYK,Iannacone M,Ivison SM,Jäck HM,Jani PK,Keller B,Kessler N,Ketelaars S,Knop L,Knopf J,Koay HF,Kobow K,Kriegsmann K,Kristyanto H,Krueger A,Kuehne JF,Kunze-Schumacher H,Kvistborg P,Kwok I,Latorre D,Lenz D,Levings MK,Lino AC,Liotta F,Long HM,Lugli E,MacDonald KN,Maggi L,Maini MK,Mair F,Manta C,Manz RA,Mashreghi MF,Mazzoni A,McCluskey J,Mei HE,Melchers F,Melzer S,Mielenz D,Monin L,Moretta L,Multhoff G,Muñoz LE,Muñoz-Ruiz M,Muscate F,Natalini A,Neumann K,Ng LG,Niedobitek A,Niemz J,Almeida LN,Notarbartolo S,Ostendorf L,Pallett LJ,Patel AA,Percin GI,Peruzzi G,Pinti M,Pockley AG,Pracht K,Prinz I,Pujol-Autonell I,Pulvirenti N,Quatrini L,Quinn KM,Radbruch H,Rhys H,Rodrigo MB,Romagnani C,Saggau C,Sakaguchi S,Sallusto F,Sanderink L,Sandrock I,Schauer C,Scheffold A,Scherer HU,Schiemann M,Schildberg FA,Schober K,Schoen J,Schuh W,Schüler T,Schulz AR,Schulz S,Schulze J,Simonetti S,Singh J,Sitnik KM,Stark R,Starossom S,Stehle C,Szelinski F,Tan L,Tarnok A,Tornack J,Tree TIM,van Beek JJP,van de Veen W,van Gisbergen K,Vasco C,Verheyden NA,von Borstel A,Ward-Hartstonge KA,Warnatz K,Waskow C,Wiedemann A,Wilharm A,Wing J,Wirz O,Wittner J,Yang JHM,Yang J

47-0168-42 was used in Flow cytometry/Cell sorting to provide the key aspects to consider when performing flow cytometry experiments.

Wed Dec 01 00:00:00 EST 2021

Isolation of polymorphonuclear neutrophils and monocytes from a single sample of human peripheral blood.

STAR protocols

Cui C,Schoenfelt KQ,Becker KM,Becker L

Published figure using CD16 monoclonal antibody (Product # 47-0168-42) in Flow Cytometry

Fri Dec 17 00:00:00 EST 2021

-------A type I IFN, prothrombotic hyperinflammatory neutrophil signature is distinct for COVID-19 ARDS--.

Wellcome open research

Reyes L,A Sanchez-Garcia M,Morrison T,Howden AJM,Watts ER,Arienti S,Sadiku P,Coelho P,Mirchandani AS,Zhang A,Hope D,Clark SK,Singleton J,Johnston S,Grecian R,Poon A,McNamara S,Harper I,Fourman MH,Brenes AJ,Pathak S,Lloyd A,Blanco GR,von Kriegsheim A,Ghesquiere B,Vermaelen W,Cologna CT,Dhaliwal K,Hirani N,Dockrell DH,Whyte MKB,Griffith D,Cantrell DA,Walmsley SR

Published figure using CD16 monoclonal antibody (Product # 47-0168-42) in Flow Cytometry

Wed Feb 15 00:00:00 EST 2023

Evolution of Antibody Immunity to SARS-CoV-2.

bioRxiv : the preprint server for biology

Gaebler C,Wang Z,Lorenzi JCC,Muecksch F,Finkin S,Tokuyama M,Cho A,Jankovic M,Schaefer-Babajew D,Oliveira TY,Cipolla M,Viant C,Barnes CO,Hurley A,Turroja M,Gordon K,Millard KG,Ramos V,Schmidt F,Weisblum Y,Jha D,Tankelevich M,Yee J,Shimeliovich I,Robbiani DF,Zhao Z,Gazumyan A,Hatziioannou T,Bjorkman PJ,Mehandru S,Bieniasz PD,Caskey M,Nussenzweig MC,Hagglof T,Schwartz RE,Bram Y,Martinez-Delgado G,Mendoza P,Breton G,Dizon J,Unson-O'Brien C,Patel R

47-0168 was used in Flow cytometry/Cell sorting to conclude that the memory B cell response to SARS-CoV-2 evolves between 1.3 and 6.2 months after infection in a manner that is consistent with antigen persistence.

Mon Jan 04 00:00:00 EST 2021

Vaccine genetics of IGHV1-2 VRC01-class broadly neutralizing antibody precursor naïve human B cells.

NPJ vaccines

Lee JH,Toy L,Kos JT,Safonova Y,Schief WR,Havenar-Daughton C,Watson CT,Crotty S

47-0168-42 was used in Flow cytometry/Cell sorting to find that IGHV1-2 alleles traditionally thought to be incompatible with VRC01-class responses are relatively common in various human populations and that germline variation within IGHV1-2 associates with gene usage frequencies in the naive BCR repertoire.

Mon Sep 06 00:00:00 EDT 2021

mRNA vaccine-elicited antibodies to SARS-CoV-2 and circulating variants.

bioRxiv : the preprint server for biology

47-0168 was used in Flow cytometry/Cell sorting to suggest that the monoclonal antibodies in clinical use should be tested against newly arising variants, and that mRNA vaccines may need to be updated periodically to avoid potential loss of clinical efficacy.

Sat Jan 30 00:00:00 EST 2021

Associations between Hypertriglyceridemia and Circulating Neutrophil Subpopulation in Patients with Dyslipidemia.

International journal of inflammation

Genkel V,Dolgushin I,Baturina I,Savochkina A,Kuznetsova A,Pykhova L,Shaposhnik I

Published figure using CD16 monoclonal antibody (Product # 47-0168-42) in Flow Cytometry

Sun Apr 24 00:00:00 EDT 2022

Immunologic mechanisms of seasonal influenza vaccination administered by microneedle patch from a randomized phase I trial.

NPJ vaccines

Rouphael NG,Lai L,Tandon S,McCullough MP,Kong Y,Kabbani S,Natrajan MS,Xu Y,Zhu Y,Wang D,O'Shea J,Sherman A,Yu T,Henry S,McAllister D,Stadlbauer D,Khurana S,Golding H,Krammer F,Mulligan MJ,Prausnitz MR

47-0168-42 was used in Flow Cytometry to compare the humoral and cellular immunologic responses in a subset of participants receiving influenza vaccination by MNP to the intramuscular (IM) route of administration.

Wed Jul 14 00:00:00 EDT 2021

Evolution of antibody immunity to SARS-CoV-2.

Nature

Gaebler C,Wang Z,Lorenzi JCC,Muecksch F,Finkin S,Tokuyama M,Cho A,Jankovic M,Schaefer-Babajew D,Oliveira TY,Cipolla M,Viant C,Barnes CO,Bram Y,Breton G,Hägglöf T,Mendoza P,Hurley A,Turroja M,Gordon K,Millard KG,Ramos V,Schmidt F,Weisblum Y,Jha D,Tankelevich M,Martinez-Delgado G,Yee J,Patel R,Dizon J,Unson-O'Brien C,Shimeliovich I,Robbiani DF,Zhao Z,Gazumyan A,Schwartz RE,Hatziioannou T,Bjorkman PJ,Mehandru S,Bieniasz PD,Caskey M,Nussenzweig MC

47-0168 was used in Flow cytometry/Cell sorting to report on the humoral memory response in a cohort of 87 individuals assessed at 1.3 and 6.2 months after infection with SARS-CoV-2.

Mon Mar 01 00:00:00 EST 2021

Convergent antibody responses to SARS-CoV-2 in convalescent individuals.

Nature

Robbiani DF,Gaebler C,Muecksch F,Lorenzi JCC,Wang Z,Cho A,Agudelo M,Barnes CO,Gazumyan A,Finkin S,Hägglöf T,Oliveira TY,Viant C,Hurley A,Hoffmann HH,Millard KG,Kost RG,Cipolla M,Gordon K,Bianchini F,Chen ST,Ramos V,Patel R,Dizon J,Shimeliovich I,Mendoza P,Hartweger H,Nogueira L,Pack M,Horowitz J,Schmidt F,Weisblum Y,Michailidis E,Ashbrook AW,Waltari E,Pak JE,Huey-Tubman KE,Koranda N,Hoffman PR,West AP,Rice CM,Hatziioannou T,Bjorkman PJ,Bieniasz PD,Caskey M,Nussenzweig MC

47-0168 was used in Flow cytometry/Cell sorting to investigate if convalescent plasma samples obtained from individuals who recover from COVID-19 contain high levels of neutralizing activity.

Sat Aug 01 00:00:00 EDT 2020

Antigen-Specific Adaptive Immunity to SARS-CoV-2 in Acute COVID-19 and Associations with Age and Disease Severity.

Cell

Rydyznski Moderbacher C,Ramirez SI,Dan JM,Grifoni A,Hastie KM,Weiskopf D,Belanger S,Abbott RK,Kim C,Choi J,Kato Y,Crotty EG,Kim C,Rawlings SA,Mateus J,Tse LPV,Frazier A,Baric R,Peters B,Greenbaum J,Ollmann Saphire E,Smith DM,Sette A,Crotty S

47-0168 was used in Flow cytometry/Cell sorting to complete a combined examination of all three branches of adaptive immunity at the level of SARS-CoV-2-specific CD4+ and CD8+ T cell and neutralizing antibody responses in acute and convalescent subjects.

Thu Nov 12 00:00:00 EST 2020

SARS-CoV-2-triggered neutrophil extracellular traps mediate COVID-19 pathology.

The Journal of experimental medicine

Veras FP,Pontelli MC,Silva CM,Toller-Kawahisa JE,de Lima M,Nascimento DC,Schneider AH,Caetité D,Tavares LA,Paiva IM,Rosales R,Colón D,Martins R,Castro IA,Almeida GM,Lopes MIF,Benatti MN,Bonjorno LP,Giannini MC,Luppino-Assad R,Almeida SL,Vilar F,Santana R,Bollela VR,Auxiliadora-Martins M,Borges M,Miranda CH,Pazin-Filho A,da Silva LLP,Cunha LD,Zamboni DS,Dal-Pizzol F,Leiria LO,Siyuan L,Batah S,Fabro A,Mauad T,Dolhnikoff M,Duarte-Neto A,Saldiva P,Cunha TM,Alves-Filho JC,Arruda E,Louzada-Junior P,Oliveira RD,Cunha FQ

Published figure using CD16 monoclonal antibody (Product # 47-0168-42) in Flow Cytometry

Mon Dec 07 00:00:00 EST 2020

IL-36 Promotes Systemic IFN-I Responses in Severe Forms of Psoriasis.

The Journal of investigative dermatology

Catapano M,Vergnano M,Romano M,Mahil SK,Choon SE,Burden AD,Young HS,Carr IM,Lachmann HJ,Lombardi G,Smith CH,Ciccarelli FD,Barker JN,Capon F

Published figure using CD16 monoclonal antibody (Product # 47-0168-42) in Flow Cytometry

Wed Apr 01 00:00:00 EDT 2020

CD14⁺CD16^- monocytes are the main precursors of osteoclasts in rheumatoid arthritis via expressing Tyro3TK.

Arthritis research & therapy

Xue J,Xu L,Zhu H,Bai M,Li X,Zhao Z,Zhong H,Cheng G,Li X,Hu F,Su Y

Published figure using CD16 monoclonal antibody (Product # 47-0168-42) in Flow Cytometry

Mon Sep 21 00:00:00 EDT 2020

NKG2C^pos NK Cells Regulate the Expansion of Cytomegalovirus-Specific CD8 T Cells.

Journal of immunology (Baltimore, Md. : 1950)

Grutza R,Moskorz W,Senff T,Bäcker E,Lindemann M,Zimmermann A,Uhrberg M,Lang PA,Timm J,Cosmovici C

47-0168-42 was used in Flow Cytometry to show that NKG2Cpos NK cells interact with HLA-Ehigh CD8 T cells, which may negatively regulate the expansion of CMV-specific CD8 T cells upon activation.

Mon Jun 01 00:00:00 EDT 2020

Circulating Neutrophils of Nonalcoholic Steatohepatitis Patients Show an Activated Phenotype and Suppress T Lymphocytes Activity.

Journal of immunology research

Antonucci L,Porcu C,Timperi E,Santini SJ,Iannucci G,Balsano C

Published figure using CD16 monoclonal antibody (Product # 47-0168-42) in Flow Cytometry

Fri May 21 00:00:00 EDT 2021

Isolation of single HIV-1 Envelope specific B cells and antibody cloning from immunized rhesus macaques.

Journal of immunological methods

Wang Z,Merkenschlager J,Chen ST,Oliveira TY,Ramos V,Gordon KM,Yao KH,Jankovic M,Nussenzweig M,Escolano A

47-0168 was used in Flow cytometry/Cell sorting to describe a protocol to identify and purify single antigen-specific macaque B cells, and subsequently clone and produce macaque monoclonal antibodies.

Sun Mar 01 00:00:00 EST 2020

Loss of testosterone impairs anti-tumor neutrophil function.

Nature communications

Markman JL,Porritt RA,Wakita D,Lane ME,Martinon D,Noval Rivas M,Luu M,Posadas EM,Crother TR,Arditi M

Published figure using CD16 monoclonal antibody (Product # 47-0168-42) in Flow Cytometry

Tue Mar 31 00:00:00 EDT 2020

A broadly neutralizing macaque monoclonal antibody against the HIV-1 V3-Glycan patch.

eLife

Wang Z,Barnes CO,Gautam R,Cetrulo Lorenzi JC,Mayer CT,Oliveira TY,Ramos V,Cipolla M,Gordon KM,Gristick HB,West AP,Nishimura Y,Raina H,Seaman MS,Gazumyan A,Martin M,Bjorkman PJ,Nussenzweig MC,Escolano A

47-0168 was used in Flow cytometry/Cell sorting to report on a monoclonal antibody, Ab1485, isolated from a macaque infected with SHIVAD8 that developed broadly neutralizing serologic activity targeting the V3-glycan region of HIV-1 Env.

Wed Oct 21 00:00:00 EDT 2020

Virus-Host Interactions Between Nonsecretors and Human Norovirus.

Cellular and molecular gastroenterology and hepatology

Lindesmith LC,Brewer-Jensen PD,Mallory ML,Jensen K,Yount BL,Costantini V,Collins MH,Edwards CE,Sheahan TP,Vinjé J,Baric RS

47-0168 was used in Flow cytometry/Cell sorting to show that GII.2 infection results in broad antibody and cellular immunity activation that persisted for at least 30 days for T cells, monocytes, and dendritic cells, and for 180 days for blocking antibody.

Fri Jul 09 00:00:00 EDT 2021

A FACS-based approach to obtain viable eosinophils from human adipose tissue.

Scientific reports

Hernandez JD,Tew BY,Li T,Gooden GC,Ghannam H,Masuda M,Madura J,Salhia B,Jacobsen EA,De Filippis E

Published figure using CD16 monoclonal antibody (Product # 47-0168-42) in Flow Cytometry

Thu Aug 06 00:00:00 EDT 2020

Convergent Antibody Responses to SARS-CoV-2 Infection in Convalescent Individuals.

bioRxiv : the preprint server for biology

Robbiani DF,Gaebler C,Muecksch F,Lorenzi JCC,Wang Z,Cho A,Agudelo M,Barnes CO,Gazumyan A,Finkin S,Hagglof T,Oliveira TY,Viant C,Hurley A,Hoffmann HH,Millard KG,Kost RG,Cipolla M,Gordon K,Bianchini F,Chen ST,Ramos V,Patel R,Dizon J,Shimeliovich I,Mendoza P,Hartweger H,Nogueira L,Pack M,Horowitz J,Schmidt F,Weisblum Y,Michailidis E,Ashbrook AW,Waltari E,Pak JE,Huey-Tubman KE,Koranda N,Hoffman PR,West AP,Rice CM,Hatziioannou T,Bjorkman PJ,Bieniasz PD,Caskey M,Nussenzweig MC

47-0168 was used in Flow cytometry/Cell sorting to conclude that most convalescent plasmas obtained from individuals who recover from COVID-19 do not contain high levels of neutralizing activity.

Fri May 22 00:00:00 EDT 2020

Immunization expands B cells specific to HIV-1 V3 glycan in mice and macaques.

Nature

Escolano A,Gristick HB,Abernathy ME,Merkenschlager J,Gautam R,Oliveira TY,Pai J,West AP,Barnes CO,Cohen AA,Wang H,Golijanin J,Yost D,Keeffe JR,Wang Z,Zhao P,Yao KH,Bauer J,Nogueira L,Gao H,Voll AV,Montefiori DC,Seaman MS,Gazumyan A,Silva M,McGuire AT,Stamatatos L,Irvine DJ,Wells L,Martin MA,Bjorkman PJ,Nussenzweig MC

47-0168-42 was used in Flow Cytometry to develop an immunogen, RC1, that facilitates the recognition of the variable loop 3 (V3)-glycan patch on the envelope protein of HIV-1 to activate B cells that express precursors of broadly neutralizing antibodies within polyclonal repertoires.

Sat Jun 01 00:00:00 EDT 2019

Progranulin prevents regulatory NK cell cytotoxicity against antiviral T cells.

JCI insight

Huang A,Shinde PV,Huang J,Senff T,Xu HC,Margotta C,Häussinger D,Willnow TE,Zhang J,Pandyra AA,Timm J,Weggen S,Lang KS,Lang PA

47-0168 was used in Flow cytometry/Cell sorting to show that progranulin (PGRN) can reduce NK cell cytotoxicity through reduction of NK cell expansion, granzyme B transcription, and NK cell-mediated lysis of target cells.

Thu Sep 05 00:00:00 EDT 2019

Outcomes of controlled human malaria infection after BCG vaccination.

Nature communications

Walk J,de Bree LCJ,Graumans W,Stoter R,van Gemert GJ,van de Vegte-Bolmer M,Teelen K,Hermsen CC,Arts RJW,Behet MC,Keramati F,Moorlag SJCFM,Yang ASP,van Crevel R,Aaby P,de Mast Q,van der Ven AJAM,Stabell Benn C,Netea MG,Sauerwein RW

47-0168 was used in Flow cytometry/Cell sorting to evaluate the induction of immunity and the protective efficacy of the anti-tuberculosis Bacillus-Calmette Guerin vaccine against a controlled human malaria infection.

Wed Feb 20 00:00:00 EST 2019

LIGHT-HVEM Signaling in Innate Lymphoid Cell Subsets Protects Against Enteric Bacterial Infection.

Cell host & microbe

Seo GY,Shui JW,Takahashi D,Song C,Wang Q,Kim K,Mikulski Z,Chandra S,Giles DA,Zahner S,Kim PH,Cheroutre H,Colonna M,Kronenberg M

47-0168-42 was used in Flow cytometry/Cell sorting to investigate if signaling through herpes virus entry mediator (HVEM) in Innate lymphoid cells 3 is important for host defense against oral infection with the bacterial pathogen Yersinia enterocolitica.

Wed Aug 08 00:00:00 EDT 2018

Ex vivo culture of human atherosclerotic plaques: A model to study immune cells in atherogenesis.

Atherosclerosis

Lebedeva A,Vorobyeva D,Vagida M,Ivanova O,Felker E,Fitzgerald W,Danilova N,Gontarenko V,Shpektor A,Vasilieva E,Margolis L

Published figure using CD16 monoclonal antibody (Product # 47-0168-42) in Flow Cytometry

Fri Dec 01 00:00:00 EST 2017

Metformin-treated cancer cells modulate macrophage polarization through AMPK-NF-κB signaling.

Oncotarget

Chiang CF,Chao TT,Su YF,Hsu CC,Chien CY,Chiu KC,Shiah SG,Lee CH,Liu SY,Shieh YS

Published figure using CD16 monoclonal antibody (Product # 47-0168-42) in Flow Cytometry

Tue Mar 28 00:00:00 EDT 2017

Pulmonary sarcoidosis is associated with high-level inducible co-stimulator (ICOS) expression on lung regulatory T cells--possible implications for the ICOS/ICOS-ligand axis in disease course and resolution.

Clinical and experimental immunology

Sakthivel P,Grunewald J,Eklund A,Bruder D,Wahlström J

Published figure using CD16 monoclonal antibody (Product # 47-0168-42) in Flow Cytometry

Mon Feb 01 00:00:00 EST 2016

Retinoic acid regulates hematopoietic development from human pluripotent stem cells.

Stem cell reports

Rönn RE,Guibentif C,Moraghebi R,Chaves P,Saxena S,Garcia B,Woods NB

Published figure using CD16 monoclonal antibody (Product # 47-0168-42) in Flow Cytometry

Tue Feb 10 00:00:00 EST 2015

Changes in natural killer cell subsets in pediatric liver transplant recipients.

Pediatric transplantation

Pham B,Piard-Ruster K,Silva R,Gallo A,Esquivel CO,Martinez OM,Krams SM

Published figure using CD16 monoclonal antibody (Product # 47-0168-42) in Flow Cytometry

Thu Mar 01 00:00:00 EST 2012

Bioinformatics

Protein Aliases: CD16-I; CD16-II; CD16a; CD16a antigen; CD16b; CD32 receptor 2; Fc fragment of IgG low affinity IIIa receptor; Fc fragment of IgG, low affinity III, receptor for (CD16); Fc fragment of IgG, low affinity IIIa, receptor (CD16a); Fc fragment of IgG, low affinity IIIb, receptor (CD16b); Fc gamma receptor III-A; Fc gamma receptor IIIa; Fc gamma receptor IIIb; Fc-gamma receptor III-2 (CD 16); Fc-gamma receptor IIIb (CD 16); Fc-gamma receptor IIIb (CD16); Fc-gamma RIII; Fc-gamma RIII-alpha; Fc-gamma RIII-beta; Fc-gamma RIIIa; fc-gamma RIIIb; FCG2; FcgammaRIIIA; FCGR2; FCGR2A; FCGR2B; FCGR2C; FcR-10; FcRII-b; FcRII-c; IgG Fc receptor III-1; IgG Fc receptor III-2; IgG Fc receptor III-A; immunoglobulin G Fc receptor III; Low affinity immunoglobulin gamma Fc region receptor III-A; Low affinity immunoglobulin gamma Fc region receptor III-B; neutrophil-specific antigen NA; RP11-5K23.1

View more View less

Gene Aliases: CD16; CD16A; CD16B; FCG3; FCGR3; FCGR3A; FCGR3B; FCGRIII; FCR-10; FCRIII; FCRIIIA; FCRIIIb; IGFR3; IMD20

View more View less

UniProt ID: (Human) P08637, (Human) O75015

View more View less

Entrez Gene ID: (Human) 2214, (Human) 2215

View more View less

Function(s)

IgG binding immunoglobulin receptor superfamily

Process(es)

immune response Fc-gamma receptor signaling pathway involved in phagocytosis regulation of immune response positive regulation of defense response to virus by host mitophagy in response to mitochondrial depolarization xenophagy

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

Learn more

CD16 Monoclonal Antibody (eBioCB16 (CB16)), APC-eFluor™ 780, eBioscience™

CD16 Antibody (47-0168-42) in Flow

Product Details

47-0168-42

Flow Cytometry (Flow)

Species Reactivity

Published species

Host/Isotype

Recommended Isotype Control

Class

Type

Clone

Conjugate

Excitation/Emission Max

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

How to use the Panel Builder

Bioinformatics

Performance Guarantee

We're here to help

Your items have has been added!