Invitrogen

CD39 Monoclonal Antibody (eBioA1 (A1)), PE, eBioscience™

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Antibody Testing Data (1)
Published Figures (13)

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CD39 Antibody (12-0399-42) in Flow

Staining of normal human peripheral blood cells with Anti-Human CD39 PE, followed by fixation and permeabilization with the Foxp3 Staining Buffers (Product # 00-5523-00), and intracellular staining with Anti-Human Foxp3 APC (Product # 17-4776-42). Cells in the lymphocyte gate were used for analysis.

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Product Details

12-0399-42

Applications

Tested Dilution

Publications

Immunohistochemistry (Frozen) (IHC (F))

View 1 publication 1 publication

Flow Cytometry (Flow)

5 µL (0.125 µg)/test

View 16 publications 16 publications

Product Specifications

Species Reactivity

Human

Published species

Human, Non-human primate

Host/Isotype

Mouse / IgG1, kappa

Recommended Isotype Control

Mouse IgG1 kappa Isotype Control (P3.6.2.8.1), PE, eBioscience™

Class

Monoclonal

Type

Antibody

Clone

eBioA1 (A1)

Conjugate

PE PE PE

Excitation/Emission Max

565/576 nm View spectra

Form

Liquid

Concentration

5 µL/Test

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.2, with 0.2% BSA

Contains

0.09% sodium azide

Storage conditions

4° C, store in dark, DO NOT FREEZE!

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_1272091

Product Specific Information

Description: The eBioA1 monoclonal antibody reacts with human CD39 also known as ectonucleoside triphosphate diphosphohydrolase 1 (ENTPD1) or NTPDase. CD39 is an integral membrane protein with two transmembrane domains and exists as a homotetramer. It is the most prominent ectoenzyme of the immune system. The function of CD39 is to effectively remove toxic extracellular ATP by converting it to ADP or AMP. CD39 is thought to work together with CD73 to hydrolyze ATP and has been well characterized on Langerhans cells. Expression of CD39 was originally identified on activated lymphocytes. Expression is also found on a subset of T cells, B cells and dendritic cells as well as weak staining on monocytes and granulocytes.

Recently, CD39 and CD73 have been found on regulatory T cells (Treg). Expression of CD39 on Treg may facilitate their entry into inflamed areas where high levels of ATP are present. Expression of CD39 on Foxp3+CD4+ cells ranges from 25-45%.

Applications Reported: This eBioA1 (A1) antibody has been reported for use in flow cytometric analysis.

Applications Tested: This eBioA1 (A1) antibody has been pre-titrated and tested flow cytometric analysis of normal human peripheral blood cells. his can be used at 5 µL (0.125 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test.

Excitation: 488-561 nm; Emission: 578 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser.

Filtration: 0.2 µm post-manufacturing filtered.

Target Information

CD39 cell surface antigen is a 70-100kD molecule expressed on peripheral blood B cells, monocytes, T cell clones, and also weakly expressed on granulocytes. CD39 has intrinsic ecto-ATPase activity. Expression of CD39 is induced on T cells and increased on B cells as a late activation antigen.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Product References

Pinpointing the tumor-specific T cells via TCR clusters.

eLife

Goncharov MM,Bryushkova EA,Sharaev NI,Skatova VD,Baryshnikova AM,Sharonov GV,Karnaukhov V,Vakhitova MT,Samoylenko IV,Demidov LV,Lukyanov S,Chudakov DM,Serebrovskaya EO

Published figure using CD39 monoclonal antibody (Product # 12-0399-42) in Flow Cytometry

Mon Apr 04 00:00:00 EDT 2022

Neoadjuvant anti-OX40 (MEDI6469) therapy in patients with head and neck squamous cell carcinoma activates and expands antigen-specific tumor-infiltrating T cells.

Nature communications

Duhen R,Ballesteros-Merino C,Frye AK,Tran E,Rajamanickam V,Chang SC,Koguchi Y,Bifulco CB,Bernard B,Leidner RS,Curti BD,Fox BA,Urba WJ,Bell RB,Weinberg AD

Published figure using CD39 monoclonal antibody (Product # 12-0399-42) in Flow Cytometry

Tue Feb 16 00:00:00 EST 2021

Molecular profiling of driver events in metastatic uveal melanoma.

Nature communications

Karlsson J,Nilsson LM,Mitra S,Alsén S,Shelke GV,Sah VR,Forsberg EMV,Stierner U,All-Eriksson C,Einarsdottir B,Jespersen H,Ny L,Lindnér P,Larsson E,Olofsson Bagge R,Nilsson JA

Published figure using CD39 monoclonal antibody (Product # 12-0399-42) in Flow Cytometry

Mon Apr 20 00:00:00 EDT 2020

Immune Suppressive Effects of Plasma-Derived Exosome Populations in Head and Neck Cancer.

Cancers

Beccard IJ,Hofmann L,Schroeder JC,Ludwig S,Laban S,Brunner C,Lotfi R,Hoffmann TK,Jackson EK,Schuler PJ,Theodoraki MN

Published figure using CD39 monoclonal antibody (Product # 12-0399-42) in Flow Cytometry

Tue Jul 21 00:00:00 EDT 2020

Circulating Exosomes Inhibit B Cell Proliferation and Activity.

Cancers

Schroeder JC,Puntigam L,Hofmann L,Jeske SS,Beccard IJ,Doescher J,Laban S,Hoffmann TK,Brunner C,Theodoraki MN,Schuler PJ

Published figure using CD39 monoclonal antibody (Product # 12-0399-42) in Flow Cytometry

Wed Jul 29 00:00:00 EDT 2020

Differential Dynamics of Regulatory T-Cell and Th17 Cell Balance in Mesenteric Lymph Nodes and Blood following Early Antiretroviral Initiation during Acute Simian Immunodeficiency Virus Infection.

Journal of virology

Yero A,Farnos O,Rabezanahary H,Racine G,Estaquier J,Jenabian MA

Published figure using CD39 monoclonal antibody (Product # 12-0399-42) in Flow Cytometry

Tue Oct 01 00:00:00 EDT 2019

Spatial and Single-Cell Transcriptional Profiling Identifies Functionally Distinct Human Dermal Fibroblast Subpopulations.

The Journal of investigative dermatology

Philippeos C,Telerman SB,Oulès B,Pisco AO,Shaw TJ,Elgueta R,Lombardi G,Driskell RR,Soldin M,Lynch MD,Watt FM

Published figure using CD39 monoclonal antibody (Product # 12-0399-42) in Flow Cytometry

Sun Apr 01 00:00:00 EDT 2018

The influence of chemotherapy on adenosine-producing B cells in patients with head and neck squamous cell carcinoma.

Oncotarget

Ziebart A,Huber U,Jeske S,Laban S,Doescher J,Hoffmann TK,Brunner C,Jackson EK,Schuler PJ

Published figure using CD39 monoclonal antibody (Product # 12-0399-42) in Flow Cytometry

Fri Jan 19 00:00:00 EST 2018

Low-dose interleukin-2 promotes STAT-5 phosphorylation, T_reg survival and CTLA-4-dependent function in autoimmune liver diseases.

Clinical and experimental immunology

Jeffery HC,Jeffery LE,Lutz P,Corrigan M,Webb GJ,Hirschfield GM,Adams DH,Oo YH

12-0399 was used in Flow cytometry/Cell sorting to establish the potential of interleukin-2 to enhance regulatory T cell therapy in autoimmune liver disease.

Thu Jun 01 00:00:00 EDT 2017

Tumor-derived exosomes regulate expression of immune function-related genes in human T cell subsets.

Scientific reports

Muller L,Mitsuhashi M,Simms P,Gooding WE,Whiteside TL

Published figure using CD39 monoclonal antibody (Product # 12-0399-42) in Flow Cytometry

Thu Feb 04 00:00:00 EST 2016

Monitoring regulatory T cells in clinical samples: consensus on an essential marker set and gating strategy for regulatory T cell analysis by flow cytometry.

Cancer immunology, immunotherapy : CII

Santegoets SJ,Dijkgraaf EM,Battaglia A,Beckhove P,Britten CM,Gallimore A,Godkin A,Gouttefangeas C,de Gruijl TD,Koenen HJ,Scheffold A,Shevach EM,Staats J,Taskén K,Whiteside TL,Kroep JR,Welters MJ,van der Burg SH

12-0399 was used in Flow cytometry/Cell sorting to exhibit a novel strategy and system for the context-dependent analysis of Tregs.

Thu Oct 01 00:00:00 EDT 2015

Critical Role for the Adenosine Pathway in Controlling Simian Immunodeficiency Virus-Related Immune Activation and Inflammation in Gut Mucosal Tissues.

Journal of virology

He T,Brocca-Cofano E,Gillespie DG,Xu C,Stock JL,Ma D,Policicchio BB,Raehtz KD,Rinaldo CR,Apetrei C,Jackson EK,Macatangay BJ,Pandrea I

12-0399-42 was used in Flow cytometry/Cell sorting to examine the role of the adenosine pathway in human immunodeficiency virus type 1/simian immunodeficiency virus infection.

Tue Sep 01 00:00:00 EDT 2015

Effect of chorioamnionitis on regulatory T cells in moderate/late preterm neonates.

Human immunology

Rueda CM,Wells CB,Gisslen T,Jobe AH,Kallapur SG,Chougnet CA

12-0399 was used in Flow cytometry/Cell sorting to show that relative to term, prematurity and chorioamnionitis decrease Treg cell functionality without affecting their frequency or phenotype.

Thu Jan 01 00:00:00 EST 2015

CD39 expression by hepatic myeloid dendritic cells attenuates inflammation in liver transplant ischemia-reperfusion injury in mice.

Hepatology (Baltimore, Md.)

Yoshida O,Kimura S,Jackson EK,Robson SC,Geller DA,Murase N,Thomson AW

12-0399 was used in Flow cytometry/Cell sorting to study whether CD39 expression on liver dendritic cells contributes to the regulation of their innate immune functions.

Sun Dec 01 00:00:00 EST 2013

Galectin-9 and IL-21 mediate cross-regulation between Th17 and Treg cells during acute hepatitis C.

PLoS pathogens

Kared H,Fabre T,Bédard N,Bruneau J,Shoukry NH

12-0399 was used in Flow cytometry/Cell sorting to longitudinally analyse CD4 helper T cell function as well as the interaction between CD4 and CD8 T cells during acute HCV infection in individuals with spontaneous resolution vs persistent viremia.

Fri Jan 17 00:00:00 EST 2014

OMIP-006: phenotypic subset analysis of human T regulatory cells via polychromatic flow cytometry.

Cytometry. Part A : the journal of the International Society for Analytical Cytology

Murdoch DM,Staats JS,Weinhold KJ

Published figure using CD39 monoclonal antibody (Product # 12-0399-42) in Flow Cytometry

Sun Apr 01 00:00:00 EDT 2012

Immunohistochemical markers for quantitative studies of neurons and glia in human neocortex.

The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society

Lyck L,Dalmau I,Chemnitz J,Finsen B,Schrøder HD

Sat Mar 01 00:00:00 EST 2008

Bioinformatics

Protein Aliases: ATP diphosphohydrolase; ATP-DPH; CD39; CD39 antigen; DKFZp686D194; DKFZp686I093; Ecto-apyrase; Ecto-ATP diphosphohydrolase 1; ecto-ATPase 1; Ecto-ATPDase 1; Ectonucleoside triphosphate diphosphohydrolase 1; FLJ40921; FLJ40959; Lymphoid cell activation antigen; NTPDase1; Nucleoside triphosphate diphosphohydrolase 1

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Gene Aliases: ATPDase; CD39; ENTPD1; NTPDase-1; SPG64

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UniProt ID: (Human) P49961

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Entrez Gene ID: (Human) 953

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Function(s)

protein binding ATP binding hydrolase activity hydrolase nucleotide phosphatase phosphatase metabolite interconversion enzyme

Process(es)

cell adhesion blood coagulation

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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CD39 Monoclonal Antibody (eBioA1 (A1)), PE, eBioscience™

CD39 Antibody (12-0399-42) in Flow

Product Details

12-0399-42

Immunohistochemistry (Frozen) (IHC (F))

Flow Cytometry (Flow)

Species Reactivity

Published species

Host/Isotype

Recommended Isotype Control

Class

Type

Clone

Conjugate

Excitation/Emission Max

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

How to use the Panel Builder

Bioinformatics

Performance Guarantee

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