Invitrogen

CD39 Monoclonal Antibody (eBioA1 (A1)), PE-Cyanine7, eBioscience™

Advanced Verification

This Antibody was verified by Cell treatment to ensure that the antibody binds to the antigen stated.

13 Published Figures

23 References

View all (78) CD39 antibodies

Datasheet

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Protocols

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FIGURE: 1 / 15

CD39 Antibody (25-0399-42) in Flow

Staining of normal human peripheral blood cells with Anti-Human CD19 APC (Product # 17-0199-42) and Mouse IgG1 K Isotype Control PE-Cyanine7 (Product # 25-4714-80) (left) or Anti-Human CD39 PE-Cyanine7 (right). Cells in the lymphocyte gate were used for analysis.

Product Image Gallery

CD39 Antibody — Figure 2 Phenotypic characterization of B cells in patients with HNSCC before and after treatment with CRT, respectively Isolated PBMC were stained for flow cytometry and examined for surface expression of ectonucleotidases CD39 and CD73. ( A ) Frequency ( n = 15) and absolute number ( n = 4) of adenosine producing B cells defined by the co-expression of CD39 and CD73. ( B ) Density plot of one representative subject showing CD39 and CD73 expression in CD19+ gated cells. Percentages of CD19+CD39+CD73+ cells are indicated in the relevant quadrant. Box plots showing reduced surface expression of CD39 and CD73 as mean fluorescence intensity (MFI) in patients treated with cisplatin/carboplatin ( C ) while bar charts before and after methotrexate treatment are show no significant difference ( D ). {TM}

Product Details

25-0399-42

Applications

Tested Dilution

Publications

Immunohistochemistry (Frozen) (IHC (F))

View 1 publication 1 publication

Flow Cytometry (Flow)

5 µL (0.25 µg)/test

View 22 publications 22 publications

Product Specifications

Species Reactivity

Human

Published species

Human

Host/Isotype

Mouse / IgG1, kappa

Recommended Isotype Control

Mouse IgG1 kappa Isotype Control (P3.6.2.8.1), PE-Cyanine7, eBioscience™

Class

Monoclonal

Type

Antibody

Clone

eBioA1 (A1)

Conjugate

PE-Cyanine7 PE-Cyanine7 PE-Cyanine7

Excitation/Emission Max

569/780 nm View spectra

Form

Liquid

Concentration

5 µL/Test

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.2, with 0.2% BSA

Contains

0.09% sodium azide

Storage conditions

4° C, store in dark, DO NOT FREEZE!

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_1582280

Product Specific Information

Description: The eBioA1 monoclonal antibody reacts with human CD39 also known as ectonucleoside triphosphate diphosphohydrolase 1 (ENTPD1) or NTPDase. CD39 is an integral membrane protein with two transmembrane domains and exists as a homotetramer. It is the most prominent ectoenzyme of the immune system. The function of CD39 is to effectively remove toxic extracellular ATP by converting it to ADP or AMP. CD39 is thought to work together with CD73 to hydrolyze ATP and has been well characterized on Langerhans cells. Expression of CD39 was originally identified on activated lymphocytes. Expression is also found on a subset of T cells, B cells and dendritic cells as well as weak staining on monocytes and granulocytes.

Recently, CD39 and CD73 have been found on regulatory T cells (Treg). Expression of CD39 on Treg may facilitate their entry into inflamed areas where high levels of ATP are present. Expression of CD39 on Foxp3+CD4+ cells ranges from 25-45%.

Applications Reported: This eBioA1 (A1) antibody has been reported for use in flow cytometric analysis.

Applications Tested: This eBioA1 (A1) antibody has been pre-titrated and tested by flow cytometric analysis of normal human peripheral blood cells. This can be used at 5 µL (0.25 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test.

Light sensitivity: This tandem dye is sensitive photo-induced oxidation. Please protect this vial and stained samples from light.

Fixation: Samples can be stored in IC Fixation Buffer (Product # 00-8222) (100 µL cell sample + 100 µL IC Fixation Buffer) or 1-step Fix/Lyse Solution (Product # 00-5333) for up to 3 days in the dark at 4°C with minimal impact on brightness and FRET efficiency/compensation. Some generalizations regarding fluorophore performance after fixation can be made, but clone specific performance should be determined empirically.

Excitation: 488-561 nm; Emission: 775 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser.

Filtration: 0.2 µm post-manufacturing filtered.

Target Information

CD39 cell surface antigen is a 70-100kD molecule expressed on peripheral blood B cells, monocytes, T cell clones, and also weakly expressed on granulocytes. CD39 has intrinsic ecto-ATPase activity. Expression of CD39 is induced on T cells and increased on B cells as a late activation antigen.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Product References

Pinpointing the tumor-specific T cells via TCR clusters.

eLife

Goncharov MM,Bryushkova EA,Sharaev NI,Skatova VD,Baryshnikova AM,Sharonov GV,Karnaukhov V,Vakhitova MT,Samoylenko IV,Demidov LV,Lukyanov S,Chudakov DM,Serebrovskaya EO

Published figure using CD39 monoclonal antibody (Product # 25-0399-42) in Flow Cytometry

Mon Apr 04 00:00:00 EDT 2022

Mesenchymal stem cells transfer mitochondria to allogeneic Tregs in an HLA-dependent manner improving their immunosuppressive activity.

Nature communications

Piekarska K,Urban-Wójciuk Z,Kurkowiak M,Pelikant-Małecka I,Schumacher A,Sakowska J,Spodnik JH,Arcimowicz Ł,Zielińska H,Tymoniuk B,Renkielska A,Siebert J,Słomińska E,Trzonkowski P,Hupp T,Marek-Trzonkowska NM

25-0399-42 was used in Flow Cytometry to demonstrate that both direct and indirect contact with allogeneic ASCs improves therapeutic potential of Tregs (Regulatory T cells).

Mon Feb 14 00:00:00 EST 2022

Neoadjuvant anti-OX40 (MEDI6469) therapy in patients with head and neck squamous cell carcinoma activates and expands antigen-specific tumor-infiltrating T cells.

Nature communications

Duhen R,Ballesteros-Merino C,Frye AK,Tran E,Rajamanickam V,Chang SC,Koguchi Y,Bifulco CB,Bernard B,Leidner RS,Curti BD,Fox BA,Urba WJ,Bell RB,Weinberg AD

Published figure using CD39 monoclonal antibody (Product # 25-0399-42) in Flow Cytometry

Tue Feb 16 00:00:00 EST 2021

Molecular profiling of driver events in metastatic uveal melanoma.

Nature communications

Karlsson J,Nilsson LM,Mitra S,Alsén S,Shelke GV,Sah VR,Forsberg EMV,Stierner U,All-Eriksson C,Einarsdottir B,Jespersen H,Ny L,Lindnér P,Larsson E,Olofsson Bagge R,Nilsson JA

25-0399 was used in Flow cytometry/Cell sorting to genomically profile metastatic tumors and infiltrating lymphocytes.

Mon Apr 20 00:00:00 EDT 2020

Molecular profiling of driver events in metastatic uveal melanoma.

Nature communications

Karlsson J,Nilsson LM,Mitra S,Alsén S,Shelke GV,Sah VR,Forsberg EMV,Stierner U,All-Eriksson C,Einarsdottir B,Jespersen H,Ny L,Lindnér P,Larsson E,Olofsson Bagge R,Nilsson JA

25-0399 was used in Flow cytometry/Cell sorting to genomically profile metastatic tumors and infiltrating lymphocytes.

Mon Apr 20 00:00:00 EDT 2020

Immune Suppressive Effects of Plasma-Derived Exosome Populations in Head and Neck Cancer.

Cancers

Beccard IJ,Hofmann L,Schroeder JC,Ludwig S,Laban S,Brunner C,Lotfi R,Hoffmann TK,Jackson EK,Schuler PJ,Theodoraki MN

25-0399 was used in Flow cytometry/Cell sorting to show the potential role of exosomes as liquid biomarkers for disease stage and level of immune suppression.

Tue Jul 21 00:00:00 EDT 2020

Immune Suppressive Effects of Plasma-Derived Exosome Populations in Head and Neck Cancer.

Cancers

Beccard IJ,Hofmann L,Schroeder JC,Ludwig S,Laban S,Brunner C,Lotfi R,Hoffmann TK,Jackson EK,Schuler PJ,Theodoraki MN

25-0399 was used in Flow cytometry/Cell sorting to show the potential role of exosomes as liquid biomarkers for disease stage and level of immune suppression.

Tue Jul 21 00:00:00 EDT 2020

Circulating Exosomes Inhibit B Cell Proliferation and Activity.

Cancers

Schroeder JC,Puntigam L,Hofmann L,Jeske SS,Beccard IJ,Doescher J,Laban S,Hoffmann TK,Brunner C,Theodoraki MN,Schuler PJ

Published figure using CD39 monoclonal antibody (Product # 25-0399-42) in Flow Cytometry

Wed Jul 29 00:00:00 EDT 2020

Circulating Exosomes Inhibit B Cell Proliferation and Activity.

Cancers

Schroeder JC,Puntigam L,Hofmann L,Jeske SS,Beccard IJ,Doescher J,Laban S,Hoffmann TK,Brunner C,Theodoraki MN,Schuler PJ

Published figure using CD39 monoclonal antibody (Product # 25-0399-42) in Flow Cytometry

Wed Jul 29 00:00:00 EDT 2020

Differential Dynamics of Regulatory T-Cell and Th17 Cell Balance in Mesenteric Lymph Nodes and Blood following Early Antiretroviral Initiation during Acute Simian Immunodeficiency Virus Infection.

Journal of virology

Yero A,Farnos O,Rabezanahary H,Racine G,Estaquier J,Jenabian MA

Published figure using CD39 monoclonal antibody (Product # 25-0399-42) in Flow Cytometry

Tue Oct 01 00:00:00 EDT 2019

Spatial and Single-Cell Transcriptional Profiling Identifies Functionally Distinct Human Dermal Fibroblast Subpopulations.

The Journal of investigative dermatology

Philippeos C,Telerman SB,Oulès B,Pisco AO,Shaw TJ,Elgueta R,Lombardi G,Driskell RR,Soldin M,Lynch MD,Watt FM

Published figure using CD39 monoclonal antibody (Product # 25-0399-42) in Flow Cytometry

Sun Apr 01 00:00:00 EDT 2018

The influence of chemotherapy on adenosine-producing B cells in patients with head and neck squamous cell carcinoma.

Oncotarget

Ziebart A,Huber U,Jeske S,Laban S,Doescher J,Hoffmann TK,Brunner C,Jackson EK,Schuler PJ

Published figure using CD39 monoclonal antibody (Product # 25-0399-42) in Flow Cytometry

Fri Jan 19 00:00:00 EST 2018

Co-expression of CD39 and CD103 identifies tumor-reactive CD8 T cells in human solid tumors.

Nature communications

Duhen T,Duhen R,Montler R,Moses J,Moudgil T,de Miranda NF,Goodall CP,Blair TC,Fox BA,McDermott JE,Chang SC,Grunkemeier G,Leidner R,Bell RB,Weinberg AD

25-0399 was used in Flow cytometry/Cell sorting to develop an approach for detecting tumour-reactive tumour-infiltrating CD8 T cells that will help define mechanisms of existing immunotherapy treatments.

Fri Jul 13 00:00:00 EDT 2018

Circulating gluten-specific FOXP3⁺CD39⁺ regulatory T cells have impaired suppressive function in patients with celiac disease.

The Journal of allergy and clinical immunology

Cook L,Munier CML,Seddiki N,van Bockel D,Ontiveros N,Hardy MY,Gillies JK,Levings MK,Reid HH,Petersen J,Rossjohn J,Anderson RP,Zaunders JJ,Tye-Din JA,Kelleher AD

25-0399 was used in Flow cytometry/Cell sorting to define whether patients with celiac disease have a dysfunction or lack of gluten-specific forkhead box protein 3+Treg cells.

Fri Dec 01 00:00:00 EST 2017

Two separate effects contribute to regulatory T cell defect in systemic lupus erythematosus patients and their unaffected relatives.

Clinical and experimental immunology

Costa N,Marques O,Godinho SI,Carvalho C,Leal B,Figueiredo AM,Vasconcelos C,Marinho A,Moraes-Fontes MF,Gomes da Costa A,Ponte C,Campanilho-Marques R,Cóias T,Martins AR,Viana JF,Lima M,Martins B,Fesel C

25-0399 was used in Flow cytometry/Cell sorting to determine the origin and role of the SLE Treg phenotype in systemic lupus erythematosus.

Fri Sep 01 00:00:00 EDT 2017

Impaired P2X1 Receptor-Mediated Adhesion in Eosinophils from Asthmatic Patients.

Journal of immunology (Baltimore, Md. : 1950)

Wright A,Mahaut-Smith M,Symon F,Sylvius N,Ran S,Bafadhel M,Muessel M,Bradding P,Wardlaw A,Vial C

25-0399 was used in Flow cytometry/Cell sorting to study the expression of P2X1, P2X4, and P2X5 receptors in healthy and asthmatic eosinophils.

Wed Jun 15 00:00:00 EDT 2016

Tumor-derived exosomes regulate expression of immune function-related genes in human T cell subsets.

Scientific reports

Muller L,Mitsuhashi M,Simms P,Gooding WE,Whiteside TL

Published figure using CD39 monoclonal antibody (Product # 25-0399-42) in Flow Cytometry

Thu Feb 04 00:00:00 EST 2016

Correlation of low CD73 expression on synovial lymphocytes with reduced adenosine generation and higher disease severity in juvenile idiopathic arthritis.

Arthritis & rheumatology (Hoboken, N.J.)

Botta Gordon-Smith S,Ursu S,Eaton S,Moncrieffe H,Wedderburn LR

25-0399 was used in Flow cytometry/Cell sorting to compare the expression of CD73 on lymphocytes from patients with juvenile idiopathic arthritis to healthy control subjects.

Sun Feb 01 00:00:00 EST 2015

PD-L1 Blockade Differentially Impacts Regulatory T Cells from HIV-Infected Individuals Depending on Plasma Viremia.

PLoS pathogens

Peligero C,Argilaguet J,Güerri-Fernandez R,Torres B,Ligero C,Colomer P,Plana M,Knobel H,García F,Meyerhans A

25-0399 was used in Flow cytometry/Cell sorting to define the consequences of ex vivo PD-L1 blockade on Treg cells from HIV-infected individuals, showing that it differentially impacts depending on plasma viremia.

Tue Dec 01 00:00:00 EST 2015

Interleukin-6 receptor blockade enhances CD39+ regulatory T cell development in rheumatoid arthritis and in experimental arthritis.

Arthritis & rheumatology (Hoboken, N.J.)

Thiolat A,Semerano L,Pers YM,Biton J,Lemeiter D,Portales P,Quentin J,Jorgensen C,Decker P,Boissier MC,Louis-Plence P,Bessis N

25-0399 was used in Flow cytometry/Cell sorting to elucidate the mechanism of action of anti-IL-6R antibody treatment by studying the effects on regulatory T cells in an experimental arthritis model and in patients with rheumatoid arthritis.

Sat Feb 01 00:00:00 EST 2014

Galectin-9 and IL-21 mediate cross-regulation between Th17 and Treg cells during acute hepatitis C.

PLoS pathogens

Kared H,Fabre T,Bédard N,Bruneau J,Shoukry NH

25-0399 was used in Flow cytometry/Cell sorting to longitudinally analyse CD4 helper T cell function as well as the interaction between CD4 and CD8 T cells during acute HCV infection in individuals with spontaneous resolution vs persistent viremia.

Fri Jan 17 00:00:00 EST 2014

Ribavirin Does Not Impair the Suppressive Activity of Foxp3(+)CD4(+)CD25(+) Regulatory T Cells.

Immune network

Lee J,Choi YS,Shin EC

25-0399 was used in Flow cytometry/Cell sorting to demonstrate that ribavirin did not attenuate the suppressive activity of Foxp3(+)CD4(+)CD25(+) Treg cells.

Fri Feb 01 00:00:00 EST 2013

OMIP-006: phenotypic subset analysis of human T regulatory cells via polychromatic flow cytometry.

Cytometry. Part A : the journal of the International Society for Analytical Cytology

Murdoch DM,Staats JS,Weinhold KJ

Published figure using CD39 monoclonal antibody (Product # 25-0399-42) in Flow Cytometry

Sun Apr 01 00:00:00 EDT 2012

Bet v 1-specific T-cell receptor/forkhead box protein 3 transgenic T cells suppress Bet v 1-specific T-cell effector function in an activation-dependent manner.

The Journal of allergy and clinical immunology

Schmetterer KG,Haiderer D,Leb-Reichl VM,Neunkirchner A,Jahn-Schmid B,Küng HJ,Schuch K,Steinberger P,Bohle B,Pickl WF

25-0399 was used in Flow cytometry/Cell sorting to demonstrate a transgenic approach to engineer customised, allergen-specific, human Treg cells, with potential implications for the treatment of allergic diseases.

Sat Jan 01 00:00:00 EST 2011

Monitoring of antigen-specific CD8 T cells in patients with type 1 diabetes treated with antiCD3 monoclonal antibodies.

Clinical immunology (Orlando, Fla.)

Cernea S,Herold KC

25-0399 was used in Flow cytometry/Cell sorting to demonstrate that class I MHC tetramers can identify antigen-specific CD8+ T-cells in patients with Type 1 diabetes.

Mon Feb 01 00:00:00 EST 2010

Immunohistochemical markers for quantitative studies of neurons and glia in human neocortex.

The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society

Lyck L,Dalmau I,Chemnitz J,Finsen B,Schrøder HD

Sat Mar 01 00:00:00 EST 2008

Bioinformatics

Protein Aliases: ATP diphosphohydrolase; ATP-DPH; CD39; CD39 antigen; DKFZp686D194; DKFZp686I093; Ecto-apyrase; Ecto-ATP diphosphohydrolase 1; ecto-ATPase 1; Ecto-ATPDase 1; Ectonucleoside triphosphate diphosphohydrolase 1; FLJ40921; FLJ40959; Lymphoid cell activation antigen; NTPDase1; Nucleoside triphosphate diphosphohydrolase 1

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Gene Aliases: ATPDase; CD39; ENTPD1; NTPDase-1; SPG64

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UniProt ID: (Human) P49961

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Entrez Gene ID: (Human) 953

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Function(s)

protein binding ATP binding hydrolase activity hydrolase nucleotide phosphatase phosphatase metabolite interconversion enzyme

Process(es)

cell adhesion blood coagulation

Performance Guarantee

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CD39 Monoclonal Antibody (eBioA1 (A1)), PE-Cyanine7, eBioscience™

CD39 Antibody (25-0399-42) in Flow

Product Details

25-0399-42

Immunohistochemistry (Frozen) (IHC (F))

Flow Cytometry (Flow)

Species Reactivity

Published species

Host/Isotype

Recommended Isotype Control

Class

Type

Clone

Conjugate

Excitation/Emission Max

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

How to use the Panel Builder

Bioinformatics

Performance Guarantee

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