Invitrogen

CD43 Monoclonal Antibody (eBioR2/60), PE, eBioscience™

Advanced Verification

This Antibody was verified by Cell treatment to ensure that the antibody binds to the antigen stated.

7 Published Figures

25 References

View all (108) CD43 antibodies

Datasheet

Protocols

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Datasheet

Protocols

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FIGURE: 1 / 9

CD43 Antibody (12-0431-82) in Flow

C57BL/6 mouse splenocytes were stained with CD45R (B220) Monoclonal Antibody, APC (Product # 17-0452-82) and 0.25 µg of Rat IgM kappa Isotype Control, PE (Product # 12-4341-82) (left) or 0.25 µg of CD43 Monoclonal Antibody, PE (right). Total viable cells were used for analysis, as determined by 7-AAD (Product # 00-6993-50).

Product Image Gallery

CD43 Antibody — Figure 1 Tissue damage suppresses LPS-induced monocytes/Mphi necroptosis. WT mice were subjected to one of the following six groups: (1) Sham for 12 h followed by PBS (vehicle) 0.2 ml i.p. (Sham+PBS); (2) Sham for 12 h followed by LPS 2 mg/kg BW in 0.2 ml of PBS i.p. for 6 h (Sham+LPS); (3) Pseudo-fracture (PF) for 12 h followed by PBS 0.2 ml i.p. (PF+PBS); and (4) PF for 12 h followed by LPS 2 mg/kg BW i.p. in 0.2 ml of PBS for 6 h (PF+LPS); (5) 0.15 ml bone-crush mixture (BCM) i.p. for 12 h followed by PBS 0.2 ml i.p. (BCM+PBS); and (6) 0.15 ml BCM i.p. for 12 h followed by LPS 2 mg/kg BW i.p. in 0.2 ml of PBS for 6 h (BCM+LPS). ( a and b ) Peritoneal Mphi (PMphi) ( a ) and monocytes ( b ) were gated by F4/80 and double stained with PE-Annexin V and 7-AAD and analyzed by flow cytometer to calculate the cell death rate. ( c and d ) Peritoneal Mphi (PMphi) ( c ) and monocytes ( d ) were double stained with CD43 and Ly6C and analyzed by flow cytometer. ( e ) Confocal microscopy shows colocalization of RIPK1 (green) and RIPK3 (red) in PMphi, indicating necrosome formation. ( f ) BMDM were pretreated with or without 40 mu l/ml of BCM for 12 h and then LPS (1 mu g/ml) for 0-24 h. The cells were double stained with PE-Annexin V and 7-AAD and analyzed by flow cytometer to calculate the cell death rate. ( g ) BMDM were treated with 40 mu l/ml of BCM for 12 h and then with or without LPS (1 mu g/ml) for 6 h. In some groups, necrostain-1 (Nec-1 s) (30 mu M), Ferrostatin-1 (Fer-1) (20 {TM}

Product Details

12-0431-82

Applications

Tested Dilution

Publications

Flow Cytometry (Flow)

0.5 µg/test

View 25 publications 25 publications

Product Specifications

Species Reactivity

Mouse

Published species

Mouse

Host/Isotype

Rat / IgM

Recommended Isotype Control

Rat IgM Isotype Control (eBRM), PE, eBioscience™

Class

Monoclonal

Type

Antibody

Clone

eBioR2/60

Conjugate

PE PE PE

Excitation/Emission Max

565/576 nm View spectra

Form

Liquid

Concentration

0.2 mg/mL

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.2

Contains

0.09% sodium azide

Storage conditions

4° C, store in dark, DO NOT FREEZE!

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_465659

Product Specific Information

Description: The eBioR2/60 monoclonal antibody reacts with mouse CD43, also known as leukosialin and sialophorin. CD43 has glycoforms of approximately 115 and 130 kDa, and is expressed by an early B cell subset in BM, thymocytes and mature T cells, NK cells, myeloid lineage cells and platelets. CD43 is reported to bind to CD54 and play a role in adhesion and B cell survival.

While the S7 and 1B11 monoclonal antibody clones detect only certain isoforms of CD43, data suggests that R2/60 detects the expression of both isoforms. It has been demonstrated that R2/60 stains the majority of CD4+ and CD8+ splenocytes, as well as a significant population of CD19+ splenocytes. However, the clones S7 and 1B11 stain only CD4+ and CD8+, or CD19+ splenocytes, respectively. This suggests that S7 and 1B11 recognize distinct epitopes which are differentially expressed by splenic B and T cells, whereas R2/60 recognizes an epitope shared by both isoforms of CD43.

Applications Reported: This eBioR2/60 antibody has been reported for use in flow cytometric analysis.

Applications Tested: This eBioR2/60 antibody has been tested by flow cytometric analysis of mouse splenocytes. This can be used at less than or equal to 0.5 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

Excitation: 488-561 nm; Emission: 578 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser.

Filtration: 0.2 µm post-manufacturing filtered.

Target Information

CD43 (leukosialin, sialophorin) is a transmembrane mucin-like protein with high negative charge, expressed on the surface of most hematopoietic cells. CD43 contributes to a repulsive barrier that interferes with cellular adhesion, however, in certain cases also promotes leukocyte aggregation. By interaction with actin-binding proteins ezrin and moesin CD43 plays a regulatory role in remodeling T-cell morphology and regulates cell-cell interactions during lymphocyte traffic. CD43 signaling both enhances LFA-1 adhesiveness and counteracts LFA-1 induction via other receptors. Expression of CD43 causes induction of functionally active tumor suppressor p53 protein, but in case of p53 and ARF deficiency CD43 promotes tumor proliferation and viability. CD43 appears to be an important modulator of leukocyte functions. Diseases associated with CD43 dysfunction include Wiscott-Aldrich Syndrome and Adenoid Basal Cell Carcinoma.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Product References

The onset of circulation triggers a metabolic switch required for endothelial to hematopoietic transition.

Cell reports

Azzoni E,Frontera V,Anselmi G,Rode C,James C,Deltcheva EM,Demian AS,Brown J,Barone C,Patelli A,Harman JR,Nicholls M,Conway SJ,Morrissey E,Jacobsen SEW,Sparrow DB,Harris AL,Enver T,de Bruijn MFTR

12-0431-82 was used in Immunocytochemistry-immunoflourescence to investigate how and when the onset of circulation affects Hematopoietic stem cells emergence.

Tue Dec 14 00:00:00 EST 2021

The evolution of zebrafish RAG2 protein is required for adapting to the elevated body temperature of the higher endothermic vertebrates.

Scientific reports

Sun A,Xu K,Liu H,Li H,Shi Y,Zhu X,Liang T,Li X,Cao X,Ji Y,Jiang T,Xu C,Liu X

Published figure using CD43 monoclonal antibody (Product # 12-0431-82) in Flow Cytometry

Thu Mar 05 00:00:00 EST 2020

Modest Declines in Proteome Quality Impair Hematopoietic Stem Cell Self-Renewal.

Cell reports

Hidalgo San Jose L,Sunshine MJ,Dillingham CH,Chua BA,Kruta M,Hong Y,Hatters DM,Signer RAJ

12-0431 was used in Flow cytometry/Cell sorting to show that hematopoietic stem cells (HSCs) are dependent on low protein synthesis to maintain proteostasis, which promotes their self-renewal.

Tue Jan 07 00:00:00 EST 2020

ImmGen report: sexual dimorphism in the immune system transcriptome.

Nature communications

Gal-Oz ST,Maier B,Yoshida H,Seddu K,Elbaz N,Czysz C,Zuk O,Stranger BE,Ner-Gaon H,Shay T

12-0431 was used in Flow cytometry/Cell sorting to show transcriptional sexual dimorphism in macrophages.

Fri Sep 20 00:00:00 EDT 2019

53BP1 cooperation with the REV7-shieldin complex underpins DNA structure-specific NHEJ.

Nature

Ghezraoui H,Oliveira C,Becker JR,Bilham K,Moralli D,Anzilotti C,Fischer R,Deobagkar-Lele M,Sanchiz-Calvo M,Fueyo-Marcos E,Bonham S,Kessler BM,Rottenberg S,Cornall RJ,Green CM,Chapman JR

Published figure using CD43 monoclonal antibody (Product # 12-0431-82) in Flow Cytometry

Wed Aug 01 00:00:00 EDT 2018

Inactivation of mTORC1 Signaling in Osterix-Expressing Cells Impairs B-cell Differentiation.

Journal of bone and mineral research : the official journal of the American Society for Bone and Mineral Research

Wang Y,Xiao M,Tao C,Chen J,Wang Z,Yang J,Chen Z,Zou Z,Liu A,Cai D,Jiang Y,Ding C,Li M,Bai X

Published figure using CD43 monoclonal antibody (Product # 12-0431-82) in Flow Cytometry

Sun Apr 01 00:00:00 EDT 2018

Lineage-Specific Genes Are Prominent DNA Damage Hotspots during Leukemic Transformation of B Cell Precursors.

Cell reports

Boulianne B,Robinson ME,May PC,Castellano L,Blighe K,Thomas J,Reid A,Müschen M,Apperley JF,Stebbing J,Feldhahn N

12-0431 was used in Flow cytometry/Cell sorting to demonstrate lineage-specific transcription predisposes genes in B cells to DNA damage.

Tue Feb 14 00:00:00 EST 2017

Tissue damage negatively regulates LPS-induced macrophage necroptosis.

Cell death and differentiation

Li Z,Scott MJ,Fan EK,Li Y,Liu J,Xiao G,Li S,Billiar TR,Wilson MA,Jiang Y,Fan J

12-0431 was used in Flow cytometry/Cell sorting to investigate the mechanism and role of trauma/tissue damage in the regulation of LPS-induced MΦ necroptosis.

Thu Sep 01 00:00:00 EDT 2016

miR-150 regulates obesity-associated insulin resistance by controlling B cell functions.

Scientific reports

Ying W,Tseng A,Chang RC,Wang H,Lin YL,Kanameni S,Brehm T,Morin A,Jones B,Splawn T,Criscitiello M,Golding MC,Bazer FW,Safe S,Zhou B

12-0431 was used in Flow cytometry/Cell sorting to demonstrate a role for miR-150 in regulating B cell functions in adipose tissue.

Mon Feb 01 00:00:00 EST 2016

Inductive interactions mediated by interplay of asymmetric signalling underlie development of adult haematopoietic stem cells.

Nature communications

Souilhol C,Gonneau C,Lendinez JG,Batsivari A,Rybtsov S,Wilson H,Morgado-Palacin L,Hills D,Taoudi S,Antonchuk J,Zhao S,Medvinsky A

12-0431 was used in Flow cytometry/Cell sorting to study how several signalling pathways interact to regulate the emergence of haematopoietic stem cells in the aorta.

Tue Mar 08 00:00:00 EST 2016

Angiotensin converting enzyme inhibitors and angiotensin II receptor antagonist attenuate tumor growth via polarization of neutrophils toward an antitumor phenotype.

Oncoimmunology

Shrestha S,Noh JM,Kim SY,Ham HY,Kim YJ,Yun YJ,Kim MJ,Kwon MS,Song DK,Hong CW

12-0431 was used in Flow cytometry/Cell sorting to investigate how modulation of Ang II pathway attenuates tumour growth via polarisation of neutrophils to an antitumoural phenotype.

Tue Jan 12 00:00:00 EST 2021

Immunomodulatory effect of captopril and local irradiation on myeloid-derived suppressor cells.

Radiation oncology journal

Cho WK,Shin SW,Kim SY,Hong CW,Choi C,Park W,Noh JM

12-0431 was used in Flow cytometry/Cell sorting to explore the anti-tumour effects of the enzyme inhibitor, Captopril.

Thu Sep 01 00:00:00 EDT 2016

Kidins220/ARMS binds to the B cell antigen receptor and regulates B cell development and activation.

The Journal of experimental medicine

Fiala GJ,Janowska I,Prutek F,Hobeika E,Satapathy A,Sprenger A,Plum T,Seidl M,Dengjel J,Reth M,Cesca F,Brummer T,Minguet S,Schamel WW

12-0431 was used in Flow cytometry/Cell sorting to study Kidins220/ARMS binding to the B cell antigen receptor and its regulation of B cell development and activation.

Mon Sep 21 00:00:00 EDT 2015

The immunosenescence-related gene Zizimin2 is associated with early bone marrow B cell development and marginal zone B cell formation.

Immunity & ageing : I & A

Matsuda T,Yanase S,Takaoka A,Maruyama M

Published figure using CD43 monoclonal antibody (Product # 12-0431-82) in Flow Cytometry

Mon Mar 02 00:00:00 EST 2015

Isolation and immunomodulatory activity of bursal peptide, a novel bursal peptide from the chicken bursa of Fabricius.

Journal of veterinary science

Liu XD,Qian Y,Jung YS,Chen PY

12-0431 was used in Flow cytometry/Cell sorting to provide novel insights into the use of a potential candidate reagent for B cell development and future immuno-pharmacological use.

Thu Sep 22 00:00:00 EDT 2016

Oral combined therapy with probiotics and alloantigen induces B cell-dependent long-lasting specific tolerance.

Journal of immunology (Baltimore, Md. : 1950)

Mercadante AC,Perobelli SM,Alves AP,Gonçalves-Silva T,Mello W,Gomes-Santos AC,Miyoshi A,Azevedo V,Faria AM,Bonomo A

12-0431 was used in Flow cytometry/Cell sorting to suggest that combined therapy is a promising strategy for prevention of GVHD with preservation of graft-versus-leukemia in allogenic HSCT.

Sat Feb 15 00:00:00 EST 2014

Cutting edge: The transcription factor Bob1 counteracts B cell activation and regulates miR-146a in B cells.

Journal of immunology (Baltimore, Md. : 1950)

Lindner JM,Kayo H,Hedlund S,Fukuda Y,Fukao T,Nielsen PJ

12-0431 was used in Flow cytometry/Cell sorting to investigate the role of Bob1 in B cells.

Thu May 15 00:00:00 EDT 2014

Conditional deletion of ferritin h in mice reduces B and T lymphocyte populations.

PloS one

Vanoaica L,Richman L,Jaworski M,Darshan D,Luther SA,Kühn LC

12-0431 was used in Flow cytometry/Cell sorting to investigate the effect of ferritin H deletion on lymphocytes, showing that it reduces B and T lymphocyte populations.

Tue Jan 13 00:00:00 EST 2015

A mouse model for inducible overexpression of Prdm14 results in rapid-onset and highly penetrant T-cell acute lymphoblastic leukemia (T-ALL).

Disease models & mechanisms

Carofino BL,Ayanga B,Justice MJ

Published figure using CD43 monoclonal antibody (Product # 12-0431-82) in Flow Cytometry

Fri Nov 01 00:00:00 EDT 2013

TDP2-dependent non-homologous end-joining protects against topoisomerase II-induced DNA breaks and genome instability in cells and in vivo.

PLoS genetics

Gómez-Herreros F,Romero-Granados R,Zeng Z,Alvarez-Quilón A,Quintero C,Ju L,Umans L,Vermeire L,Huylebroeck D,Caldecott KW,Cortés-Ledesma F

Published figure using CD43 monoclonal antibody (Product # 12-0431-82) in Flow Cytometry

Thu Jun 13 00:00:00 EDT 2013

IKKα-mediated signaling circuitry regulates early B lymphopoiesis during hematopoiesis.

Blood

Balkhi MY,Willette-Brown J,Zhu F,Chen Z,Liu S,Guttridge DC,Karin M,Hu Y

12-0431-82 was used in Flow Cytometry to demonstrate that IKKα regulates B-lineage commitment via combined canonical and noncanonical NF-κB transcriptional activities to target Pax5 expression during hematopoiesis.

Thu Jun 07 00:00:00 EDT 2012

Nucleoside salvage pathway kinases regulate hematopoiesis by linking nucleotide metabolism with replication stress.

The Journal of experimental medicine

Austin WR,Armijo AL,Campbell DO,Singh AS,Hsieh T,Nathanson D,Herschman HR,Phelps ME,Witte ON,Czernin J,Radu CG

12-0431 was used in Flow cytometry/Cell sorting to investigate a functional link between nucleotide deficiency, replication stress, and two enzymes of the nucleoside salvage pathway: deoxycytidine kinase and thymidine kinase.

Mon Nov 19 00:00:00 EST 2012

Mouse Ly49G2+ NK cells dominate early responses during both immune reconstitution and activation independently of MHC.

Blood

Barao I,Alvarez M,Ames E,Orr MT,Stefanski HE,Blazar BR,Lanier LL,Anderson SK,Redelman D,Murphy WJ

12-0431 was used in Flow cytometry/Cell sorting to show that during reconstitution after HSCT and various activation stimuli, preferential expansion of the Ly49G2 receptor on NK cells occurs independently of MHC haplotype.

Thu Jun 30 00:00:00 EDT 2011

Hydrodynamic delivery of human IL-15 cDNA increases murine natural killer cell recovery after syngeneic bone marrow transplantation.

Biology of blood and marrow transplantation : journal of the American Society for Blood and Marrow Transplantation

Barao I,Alvarez M,Redelman D,Weiss JM,Ortaldo JR,Wiltrout RH,Murphy WJ

12-0431 was used in Flow cytometry/Cell sorting to highlight cytokine gene transfer-based methods as a potential platform for natural killer cell recovery after bone marrow transplantation in mice.

Thu Dec 01 00:00:00 EST 2011

Homologous recombination is necessary for normal lymphocyte development.

Molecular and cellular biology

Caddle LB,Hasham MG,Schott WH,Shirley BJ,Mills KD

12-0431 was used in Flow cytometry/Cell sorting to demonstrate a key role for HR in lymphoid development and suggest that Xrcc2 defects could underlie some human primary immunodeficiencies.

Tue Apr 01 00:00:00 EDT 2008

Bioinformatics

Protein Aliases: 3E8 antigen; B-cell differentiation antigen LP-3; CD43; Leukocyte sialoglycoprotein; Leukosialin; Ly-48; Lymphocyte antigen 48; Sialophorin; sialophorin gpL115

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Gene Aliases: A630014B01Rik; Cd43; Galgp; Ly-48; Ly48; Spn

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UniProt ID: (Mouse) P15702

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Entrez Gene ID: (Mouse) 20737

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Function(s)

protein binding protein domain specific binding

Process(es)

response to protozoan negative regulation of type IV hypersensitivity positive regulation of protein phosphorylation negative regulation of cell adhesion cell surface receptor signaling pathway T cell costimulation positive regulation of T cell proliferation negative regulation of T cell proliferation positive regulation of tumor necrosis factor biosynthetic process defense response to bacterium negative thymic T cell selection regulation of defense response to virus regulation of immune response negative regulation of T cell activation thymocyte aggregation apoptotic signaling pathway

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CD43 Monoclonal Antibody (eBioR2/60), PE, eBioscience™

CD43 Antibody (12-0431-82) in Flow

Product Details

12-0431-82

Flow Cytometry (Flow)

Species Reactivity

Published species

Host/Isotype

Recommended Isotype Control

Class

Type

Clone

Conjugate

Excitation/Emission Max

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

How to use the Panel Builder

Bioinformatics

Performance Guarantee

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