Invitrogen

CD43 Monoclonal Antibody (eBioR2/60), eBioscience™

8 Published Figures

12 References

View all (108) CD43 antibodies

Datasheet

Protocols

Questions & Answers

Datasheet

Protocols

Questions & Answers

Antibody Testing Data (1)
Published Figures (8)

FIGURE: 1 / 9

CD43 Antibody (14-0431-82) in Flow

Staining of C57Bl/6 splenocytes with 0.25 µg of Rat IgM Isotype Control Purified (Product # 14-4341-82) (open histogram) or 0.25 µg of Anti-Mouse CD43 Purified (filled histogram) followed by Anti-Rat IgM PE (Product # 12-0990-82). Cells in the lymphocyte gate were used for analysis.

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Product Details

14-0431-82

Applications

Tested Dilution

Publications

Western Blot (WB)

Assay-Dependent

Flow Cytometry (Flow)

0.5 µg/test

View 12 publications 12 publications

Immunoprecipitation (IP)

Assay-Dependent

Product Specifications

Species Reactivity

Mouse

Published species

Mouse

Host/Isotype

Rat / IgM

Class

Monoclonal

Type

Antibody

Clone

eBioR2/60

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.5 mg/mL

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.2

Contains

0.09% sodium azide

Storage conditions

4° C

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_467241

Product Specific Information

Description: The eBioR2/60 monoclonal antibody reacts with mouse CD43, also known as leukosialin and sialophorin. CD43 has glycoforms of approximately 115 and 130 kDa, and is expressed by an early B cell subset in BM, thymocytes and mature T cells, NK cells, myeloid lineage cells and platelets. CD43 is reported to bind to CD54 and play a role in adhesion and B cell survival.

While the S7 and 1B11 monoclonal antibody clones detect only certain isoforms of CD43, data suggests that R2/60 detects the expression of both isoforms. It has been demonstrated that R2/60 stains the majority of CD4+ and CD8+ splenocytes, as well as a significant population of CD19+ splenocytes. However, the clones S7 and 1B11 stain only CD4+ and CD8+, or CD19+ splenocytes, respectively. This suggests that S7 and 1B11 recognize distinct epitopes which are differentially expressed by splenic B and T cells, whereas R2/60 recognizes an epitope shared by both isoforms of CD43.

Applications Reported: This eBioR2/60 antibody has been reported for use in flow cytometric analysis, immunoprecipitation, and immunoblotting (WB). (Please use Functional Grade eBioR2/60 (Product # 16-0431) in functional assays).

Applications Tested: This eBioR2/60 antibody has been tested by flow cytometric analysis of mouse splenocytes. This can be used at less than or equal to 0.5 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

Purity: Greater than 90%, as determined by SDS-PAGE.

Aggregation: Less than 10%, as determined by HPLC.

Filtration: 0.2 µm post-manufacturing filtered.

Target Information

CD43 (leukosialin, sialophorin) is a transmembrane mucin-like protein with high negative charge, expressed on the surface of most hematopoietic cells. CD43 contributes to a repulsive barrier that interferes with cellular adhesion, however, in certain cases also promotes leukocyte aggregation. By interaction with actin-binding proteins ezrin and moesin CD43 plays a regulatory role in remodeling T-cell morphology and regulates cell-cell interactions during lymphocyte traffic. CD43 signaling both enhances LFA-1 adhesiveness and counteracts LFA-1 induction via other receptors. Expression of CD43 causes induction of functionally active tumor suppressor p53 protein, but in case of p53 and ARF deficiency CD43 promotes tumor proliferation and viability. CD43 appears to be an important modulator of leukocyte functions. Diseases associated with CD43 dysfunction include Wiscott-Aldrich Syndrome and Adenoid Basal Cell Carcinoma.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

The evolution of zebrafish RAG2 protein is required for adapting to the elevated body temperature of the higher endothermic vertebrates.

Scientific reports

Sun A,Xu K,Liu H,Li H,Shi Y,Zhu X,Liang T,Li X,Cao X,Ji Y,Jiang T,Xu C,Liu X

14-0431-82 was used in Flow Cytometry to illustrate that the evolution of zebrafish RAG2 protein is required for adapting to the elevated body temperature of the higher endothermic vertebrates.

Thu Mar 05 00:00:00 EST 2020

The evolution of zebrafish RAG2 protein is required for adapting to the elevated body temperature of the higher endothermic vertebrates.

Scientific reports

Sun A,Xu K,Liu H,Li H,Shi Y,Zhu X,Liang T,Li X,Cao X,Ji Y,Jiang T,Xu C,Liu X

14-0431-82 was used in Flow Cytometry to illustrate that the evolution of zebrafish RAG2 protein is required for adapting to the elevated body temperature of the higher endothermic vertebrates.

Thu Mar 05 00:00:00 EST 2020

A Subset of Type I Conventional Dendritic Cells Controls Cutaneous Bacterial Infections through VEGFα-Mediated Recruitment of Neutrophils.

Immunity

Janela B,Patel AA,Lau MC,Goh CC,Msallam R,Kong WT,Fehlings M,Hubert S,Lum J,Simoni Y,Malleret B,Zolezzi F,Chen J,Poidinger M,Satpathy AT,Briseno C,Wohn C,Malissen B,Murphy KM,Maini AA,Vanhoutte L,Guilliams M,Vial E,Hennequin L,Newell E,Ng LG,Musette P,Yona S,Hacini-Rachinel F,Ginhoux F

14-0431 was used in Flow cytometry/Cell sorting to show that skin cDC1s are essential regulators of the innate response in cutaneous immunity and have roles beyond classical antigen presentation.

Tue Apr 16 00:00:00 EDT 2019

53BP1 cooperation with the REV7-shieldin complex underpins DNA structure-specific NHEJ.

Nature

Ghezraoui H,Oliveira C,Becker JR,Bilham K,Moralli D,Anzilotti C,Fischer R,Deobagkar-Lele M,Sanchiz-Calvo M,Fueyo-Marcos E,Bonham S,Kessler BM,Rottenberg S,Cornall RJ,Green CM,Chapman JR

Published figure using CD43 monoclonal antibody (Product # 14-0431-82) in Flow Cytometry

Wed Aug 01 00:00:00 EDT 2018

Inactivation of mTORC1 Signaling in Osterix-Expressing Cells Impairs B-cell Differentiation.

Journal of bone and mineral research : the official journal of the American Society for Bone and Mineral Research

Wang Y,Xiao M,Tao C,Chen J,Wang Z,Yang J,Chen Z,Zou Z,Liu A,Cai D,Jiang Y,Ding C,Li M,Bai X

Published figure using CD43 monoclonal antibody (Product # 14-0431-82) in Flow Cytometry

Sun Apr 01 00:00:00 EDT 2018

Identification of Distinct Unmutated Chronic Lymphocytic Leukemia Subsets in Mice Based on Their T Cell Dependency.

Frontiers in immunology

Pal Singh S,de Bruijn MJW,de Almeida MP,Meijers RWJ,Nitschke L,Langerak AW,Pillai SY,Stadhouders R,Hendriks RW

14-0431-81 was used in Flow Cytometry to provide evidence for two distinct unmutated CLL subsets with a specific transcriptional signature: one is T cell-independent and B-1 cell-derived while the other arises upon antigen stimulation in the context of T-cell help.

Mon Sep 30 00:00:00 EDT 2019

TLR4 signals in B lymphocytes are transduced via the B cell antigen receptor and SYK.

The Journal of experimental medicine

Schweighoffer E,Nys J,Vanes L,Smithers N,Tybulewicz VLJ

14043182 was used in flow cytometry to show that TLR4 signals through the BCR leading to activation of SYK, ERK, and AKT as well as through MYD88 leading to activation of NFkappaB

Mon May 01 00:00:00 EDT 2017

Defective germinal center B-cell response and reduced arthritic pathology in microRNA-29a-deficient mice.

Cellular and molecular life sciences : CMLS

van Nieuwenhuijze A,Dooley J,Humblet-Baron S,Sreenivasan J,Koenders M,Schlenner SM,Linterman M,Liston A

14043182 was used in flow cytometry to evaluate miR-29a in B cells as a potential therapeutic target in arthritis

Thu Jun 01 00:00:00 EDT 2017

Functional characterization of the lysosomal membrane protein TMEM192 in mice.

Oncotarget

Nguyen TL,Schneppenheim J,Rudnik S,Lüllmann-Rauch R,Bernreuther C,Hermans-Borgmeyer I,Glatzel M,Saftig P,Schröder B

14-0431-82 was used in Flow Cytometry to conclude that under basal conditions in vivo the loss of TMEM192 can be efficiently compensated by alternative pathways.

Tue Jul 04 00:00:00 EDT 2017

The immunosenescence-related gene Zizimin2 is associated with early bone marrow B cell development and marginal zone B cell formation.

Immunity & ageing : I & A

Matsuda T,Yanase S,Takaoka A,Maruyama M

Published figure using CD43 monoclonal antibody (Product # 14-0431-82) in Flow Cytometry

Mon Mar 02 00:00:00 EST 2015

A mouse model for inducible overexpression of Prdm14 results in rapid-onset and highly penetrant T-cell acute lymphoblastic leukemia (T-ALL).

Disease models & mechanisms

Carofino BL,Ayanga B,Justice MJ

Published figure using CD43 monoclonal antibody (Product # 14-0431-82) in Flow Cytometry

Fri Nov 01 00:00:00 EDT 2013

TDP2-dependent non-homologous end-joining protects against topoisomerase II-induced DNA breaks and genome instability in cells and in vivo.

PLoS genetics

Gómez-Herreros F,Romero-Granados R,Zeng Z,Alvarez-Quilón A,Quintero C,Ju L,Umans L,Vermeire L,Huylebroeck D,Caldecott KW,Cortés-Ledesma F

Published figure using CD43 monoclonal antibody (Product # 14-0431-82) in Flow Cytometry

Thu Jun 13 00:00:00 EDT 2013

Generation and evaluation of an IPTG-regulated version of Vav-gene promoter for mouse transgenesis.

PloS one

Grespi F,Ottina E,Yannoutsos N,Geley S,Villunger A

14-0431 was used in Flow cytometry/Cell sorting to evaluate the suitability of the lac-operon/lac-repressor (lacO/lacI) system for the regulation of the Vav-gene promoter, and the importance of IPTG.

Mon Mar 21 00:00:00 EDT 2011

Bioinformatics

Protein Aliases: 3E8 antigen; B-cell differentiation antigen LP-3; CD43; Leukocyte sialoglycoprotein; Leukosialin; Ly-48; Lymphocyte antigen 48; Sialophorin; sialophorin gpL115

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Gene Aliases: A630014B01Rik; Cd43; Galgp; Ly-48; Ly48; Spn

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UniProt ID: (Mouse) P15702

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Entrez Gene ID: (Mouse) 20737

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Function(s)

protein binding protein domain specific binding

Process(es)

response to protozoan negative regulation of type IV hypersensitivity positive regulation of protein phosphorylation negative regulation of cell adhesion cell surface receptor signaling pathway T cell costimulation positive regulation of T cell proliferation negative regulation of T cell proliferation positive regulation of tumor necrosis factor biosynthetic process defense response to bacterium negative thymic T cell selection regulation of defense response to virus regulation of immune response negative regulation of T cell activation thymocyte aggregation apoptotic signaling pathway

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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CD43 Monoclonal Antibody (eBioR2/60), eBioscience™

CD43 Antibody (14-0431-82) in Flow

Product Details

14-0431-82

Western Blot (WB)

Flow Cytometry (Flow)

Immunoprecipitation (IP)

Species Reactivity

Published species

Host/Isotype

Class

Type

Clone

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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