Invitrogen

CD45RB Monoclonal Antibody (C363.16A), PE, eBioscience™

1 Published Figure

22 References

View all (44) CD45RB antibodies

Datasheet

Protocols

Questions & Answers

Datasheet

Protocols

Questions & Answers

Antibody Testing Data (1)
Published Figures (1)

CD45RB Antibody in Flow Cytometry (Flow)

FIGURE: 1 / 2

CD45RB Antibody (12-0455-82) in Flow

Staining of C57BL/6 splenocytes with 0.25 µg of Rat IgG2a kappa Isotype Control PE (Product # 12-4321-80) (open histogram) or 0.125 µg of Anti-Mouse CD45RB PE (filled histogram). Total viable cells were used for analysis.

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Product Details

12-0455-82

Applications

Tested Dilution

Publications

Immunocytochemistry (ICC/IF)

View 1 publication 1 publication

Flow Cytometry (Flow)

0.25 µg/test

View 21 publications 21 publications

Product Specifications

Species Reactivity

Mouse

Published species

Mouse

Host/Isotype

Rat / IgG2a, kappa

Recommended Isotype Control

Rat IgG2a kappa Isotype Control (eBR2a), PE, eBioscience™

Class

Monoclonal

Type

Antibody

Clone

C363.16A

Conjugate

PE PE PE

Excitation/Emission Max

565/576 nm View spectra

Form

Liquid

Concentration

0.2 mg/mL

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.2

Contains

0.09% sodium azide

Storage conditions

4° C, store in dark, DO NOT FREEZE!

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_465681

Product Specific Information

Description: The C363.16A monoclonal antibody reacts with the exon B-dependent isoform of mouse CD45. This CD45 isoform is expressed by thymocytes, peripheral B cells and a subpopulation of T cells at high density and by dendritic cells and macrophages at low density. CD45 is a protein tyrosine phosphatase and serves an important role in leukocyte signal transduction.

Applications Reported: The C363.16A antibody has been reported for use in flow cytometric analysis.

Applications Tested: The C363.16A antibody has been tested by flow cytometric analysis of mouse splenocytes. This can be used at less than or equal to 0.25 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

Excitation: 488-561 nm; Emission: 578 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser.

Filtration: 0.2 µm post-manufacturing filtered.

Target Information

CD45RB is a receptor-type protein tyrosine phosphatase, CD45 glycoprotein. CD45 is crucial in lymphocyte development and antigen signaling, serving as an important regulator of Src-family kinases, promotes cell survival by modulating integrin-mediated signal transduction pathway and is also involved in DNA fragmentation during apoptosis. CD45 isoforms differ in their extracellular domains, but share identical transmembrane and cytoplasmic domains. CD45 isoforms differ in their ability to translocate into the glycosphingolipid-enriched membrane domains and their expression depends on cell type and physiological state of the cell. CD45RB is expressed in microglia and inflammatory cells. In addition to involvement in immunoreceptor signaling, CD45 is important in promoting cell survival by modulating integrin-mediated signal transduction pathway and is involved in DNA fragmentation during apoptosis. CD45 is expressed on cells of the human hematopoietic lineage with the exception of mature red cells.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Product References

TNF hampers intestinal tissue repair in colitis by restricting IL-22 bioavailability.

Mucosal immunology

Ninnemann J,Winsauer C,Bondareva M,Kühl AA,Lozza L,Durek P,Lissner D,Siegmund B,Kaufmann SHE,Mashreghi MF,Nedospasov SA,Kruglov AA

12-0455-82 was used in Immunocytochemistry to show how anti-TNF therapy induces mucosal healing by increasing IL-22 availability and implicates new therapeutic opportunities for IBD.

Fri Apr 01 00:00:00 EDT 2022

Mitochondrial transcription factor A in RORγt⁺ lymphocytes regulate small intestine homeostasis and metabolism.

Nature communications

Fu Z,Dean JW,Xiong L,Dougherty MW,Oliff KN,Chen ZE,Jobin C,Garrett TJ,Zhou L

12-0455-82 was used in Flow Cytometry to show that IL-22, a cytokine produced by RORγt+ lymphocytes inhibits IL-13-induced tuft cell differentiation in vitro, and suppresses the tuft cell-type 2 immune circuit and small intestine lengthening in vivo, highlighting its key role in gut tissue remodeling.

Thu Jul 22 00:00:00 EDT 2021

Butyrophilin-like proteins display combinatorial diversity in selecting and maintaining signature intraepithelial γδ T cell compartments.

Nature communications

Jandke A,Melandri D,Monin L,Ushakov DS,Laing AG,Vantourout P,East P,Nitta T,Narita T,Takayanagi H,Feederle R,Hayday A

Published figure using CD45RB monoclonal antibody (Product # 12-0455-82) in Flow Cytometry

Tue Jul 28 00:00:00 EDT 2020

Modulation of autoimmune pathogenesis by T cell-triggered inflammatory cell death.

Nature communications

Sasaki K,Himeno A,Nakagawa T,Sasaki Y,Kiyonari H,Iwai K

12-0455-82 was used in Flow Cytometry to show that T cell-mediated killing and sequential autoinflammation are common and crucial for pathogenic diversity during T cell-mediated autoimmune responses.

Wed Aug 28 00:00:00 EDT 2019

CD69 enhances immunosuppressive function of regulatory T-cells and attenuates colitis by prompting IL-10 production.

Cell death & disease

Yu L,Yang F,Zhang F,Guo D,Li L,Wang X,Liang T,Wang J,Cai Z,Jin H

Wed Sep 05 00:00:00 EDT 2018

β7-Integrin exacerbates experimental DSS-induced colitis in mice by directing inflammatory monocytes into the colon.

Mucosal immunology

Schippers A,Muschaweck M,Clahsen T,Tautorat S,Grieb L,Tenbrock K,Gaßler N,Wagner N

12-0455 was used in Flow cytometry/Cell sorting to elucidate the significance of β7-integrin expression on innate immune cells for the pathogenesis of inflammatory bowel disease.

Tue Mar 01 00:00:00 EST 2016

Foxp3(+) T cells expressing RORγt represent a stable regulatory T-cell effector lineage with enhanced suppressive capacity during intestinal inflammation.

Mucosal immunology

Yang BH,Hagemann S,Mamareli P,Lauer U,Hoffmann U,Beckstette M,Föhse L,Prinz I,Pezoldt J,Suerbaum S,Sparwasser T,Hamann A,Floess S,Huehn J,Lochner M

12-0455 was used in Flow cytometry/Cell sorting to demonstrate that Foxp3(+) T cells expressing retinoic acid-related orphan receptor-γt, represent a stable regulatory T cell lineage with a highly suppressive phenotype in vivo.

Tue Mar 01 00:00:00 EST 2016

Loss of the Protein Tyrosine Phosphatase PTPN22 Reduces Mannan-Induced Autoimmune Arthritis in SKG Mice.

Journal of immunology (Baltimore, Md. : 1950)

Sood S,Brownlie RJ,Garcia C,Cowan G,Salmond RJ,Sakaguchi S,Zamoyska R

12-0455 was used in Flow cytometry/Cell sorting to show that small perturbations in TCR signal transduction pathways can have profound consequences on T cell differentiation and the development of autoimmune disease.

Fri Jul 15 00:00:00 EDT 2016

Calcitonin gene-related peptide regulates type IV hypersensitivity through dendritic cell functions.

PloS one

Mikami N,Sueda K,Ogitani Y,Otani I,Takatsuji M,Wada Y,Watanabe K,Yoshikawa R,Nishioka S,Hashimoto N,Miyagi Y,Fukada S,Yamamoto H,Tsujikawa K

12-0455 was used in an ELISA assay to study how calcionin gene-related peptide (CGRP) affects dendritic cells in adaptive immunity, showing that CGRP suppresses Th1 cell differentiation via inhibition of IL-12 and down regulates the expression of chemokine receptor and its ligand.

Mon Sep 15 00:00:00 EDT 2014

Cd72(c) is a modifier gene that regulates Fas(lpr)-induced autoimmune disease.

Journal of immunology (Baltimore, Md. : 1950)

Xu M,Hou R,Sato-Hayashizaki A,Man R,Zhu C,Wakabayashi C,Hirose S,Adachi T,Tsubata T

12-0455 was used in Flow cytometry/Cell sorting to suggest that Cd72(c) is a crucial modifier gene that regulates Fas(lpr)-induced autoimmune disease by reducing B cell signal regulation.

Sat Jun 01 00:00:00 EDT 2013

Nod2 activates NF-kB in CD4+ T cells but its expression is dispensable for T cell-induced colitis.

PloS one

Zanello G,Goethel A,Forster K,Geddes K,Philpott DJ,Croitoru K

12-0455 was used in Flow cytometry/Cell sorting to show how Nod2 activates NF-kB in CD4+ T cells, but its expression is dispensable for T cell-induced colitis.

Thu Jul 31 00:00:00 EDT 2014

Type I interferons maintain Foxp3 expression and T-regulatory cell functions under inflammatory conditions in mice.

Gastroenterology

Lee SE,Li X,Kim JC,Lee J,González-Navajas JM,Hong SH,Park IK,Rhee JH,Raz E

12-0455 was used in Flow cytometry/Cell sorting to study the role of type I interferons during intestinal homeostasis regulation in a T cell-induced colitis mouse model.

Sun Jul 01 00:00:00 EDT 2012

Alterations of peripheral CD4+CD25+Foxp3+ T regulatory cells in mice with STZ-induced diabetes.

Cellular & molecular immunology

Zhen Y,Sun L,Liu H,Duan K,Zeng C,Zhang L,Jin D,Peng J,Ding W,Zhao Y

12-0455 was used in Flow cytometry/Cell sorting to investigate the effect of hyperglycemia on CD4+CD25+Tregs, showing alterations of peripheral CD4+CD25+Foxp3+ Tregs in STZ-induced diabetes.

Sun Jan 01 00:00:00 EST 2012

Indoleamine 2,3-dioxygenase and regulatory dendritic cells contribute to the allograft protection induced by infusion of donor-specific splenic stromal cells.

Cellular & molecular immunology

Liu L,Duan L,Gong M,Dai H,Gong Q,Zheng F,Tan Z,Wang C,Gong F,Fang M

12-0455 was used in Flow cytometry/Cell sorting to suggest that donor-derived splenic stromal cells could be used as a therapeutic target to promote transplantation tolerance.

Sat Jan 01 00:00:00 EST 2011

Identification of a novel antigen cross-presenting cell type in spleen.

Journal of cellular and molecular medicine

Tan JK,Quah BJ,Griffiths KL,Periasamy P,Hey YY,O'Neill HC

12-0455 was used in Flow cytometry/Cell sorting to study a novel DC type produced by in vitro haematopoiesis which has distinct antigen-presenting potential and reflects a DC subset present also in vivo in spleen.

Sun May 01 00:00:00 EDT 2011

CCR6 marks regulatory T cells as a colon-tropic, IL-10-producing phenotype.

Journal of immunology (Baltimore, Md. : 1950)

Kitamura K,Farber JM,Kelsall BL

12-0455 was used in Flow cytometry/Cell sorting to conclude that CCR6 expression on Treg cells was required for the full function of Treg cell-mediated suppression in a model of colitis.

Wed Sep 15 00:00:00 EDT 2010

c-Rel is required for the development of thymic Foxp3+ CD4 regulatory T cells.

The Journal of experimental medicine

Isomura I,Palmer S,Grumont RJ,Bunting K,Hoyne G,Wilkinson N,Banerjee A,Proietto A,Gugasyan R,Wu L,McNally A,Steptoe RJ,Thomas R,Shannon MF,Gerondakis S

12-0455 was used in Flow cytometry/Cell sorting to demonstrate that c-Rel is important for both the thymic development and peripheral homeostatic proliferation of T reg cells in mice.

Mon Dec 21 00:00:00 EST 2009

Epigenetic regulation of Foxp3 expression in regulatory T cells by DNA methylation.

Journal of immunology (Baltimore, Md. : 1950)

Lal G,Zhang N,van der Touw W,Ding Y,Ju W,Bottinger EP,Reid SP,Levy DE,Bromberg JS

12-0455 was used in Flow cytometry/Cell sorting to investigate Foxp3 as the master switch for Tregs, showing that expression is regulated by DNA methylation in Tregs.

Thu Jan 01 00:00:00 EST 2009

GITR Blockade Facilitates Treg Mediated Allograft Survival.

Transplantation

Sonawane SB,Kim JI,Lee MK,Lee SH,Duff PE,Moore DJ,Lian MM,Deng S,Choi Y,Yeh H,Caton AJ,Markmann JF

12-0455 was used in Flow cytometry/Cell sorting to study how inflammation and other innate immune signals may activate antigen presenting cells to up-regulate GITR.

Fri Nov 27 00:00:00 EST 2009

Primary activation of antigen-specific naive CD4+ and CD8+ T cells following intranasal vaccination with recombinant bacteria.

Infection and immunity

Ciabattini A,Pettini E,Andersen P,Pozzi G,Medaglini D

12-0455 was used in Flow cytometry/Cell sorting to study the primary activation of T cells following mucosal immunisation with recombinant Streptococcus gordonii.

Mon Dec 01 00:00:00 EST 2008

IFN-gamma acts directly on activated CD4+ T cells during mycobacterial infection to promote apoptosis by inducing components of the intracellular apoptosis machinery and by inducing extracellular proapoptotic signals.

Journal of immunology (Baltimore, Md. : 1950)

Li X,McKinstry KK,Swain SL,Dalton DK

12-0455 was used in Flow cytometry/Cell sorting to investigate IFN-γ-mediated apoptosis mechanisms of CD4+ T Cells during mycobacterial infection, which operate through induction of intra- and extracellular apoptosis signals.

Sun Jul 15 00:00:00 EDT 2007

Light chain editing generates polyreactive antibodies in chronic graft-versus-host reaction.

The Journal of experimental medicine

Witsch EJ,Cao H,Fukuyama H,Weigert M

12-0455 was used in Flow cytometry/Cell sorting to investigate the structure, specificity, and origin of the induced autoantibodies in chronic graft-versus-host disease, showing that light chain editing generates polyreactive antibodies.

Mon Jul 10 00:00:00 EDT 2006

Bioinformatics

Protein Aliases: CD45; L-CA; Leukocyte common antigen; Ly-5; Lymphocyte antigen 5; lymphocyte common antigen; Receptor-type tyrosine-protein phosphatase C; T200

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Gene Aliases: B220; Cd45; CD45R; L-CA; loc; Ly-5; Lyt-4; Ptprc; T200

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UniProt ID: (Mouse) P06800

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Entrez Gene ID: (Mouse) 19264

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Function(s)

phosphoprotein phosphatase activity protein tyrosine phosphatase activity protein binding heparin binding hydrolase activity phosphatase activity protein kinase binding heparan sulfate proteoglycan binding protein phosphatase protein modifying enzyme

Process(es)

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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CD45RB Monoclonal Antibody (C363.16A), PE, eBioscience™

CD45RB Antibody (12-0455-82) in Flow

Product Details

12-0455-82

Immunocytochemistry (ICC/IF)

Flow Cytometry (Flow)

Species Reactivity

Published species

Host/Isotype

Recommended Isotype Control

Class

Type

Clone

Conjugate

Excitation/Emission Max

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

How to use the Panel Builder

Bioinformatics

Performance Guarantee

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