Invitrogen

CD45RB Monoclonal Antibody (C363.16A), FITC, eBioscience™

1 Published Figure

9 References

View all (44) CD45RB antibodies

Datasheet

Protocols

Questions & Answers

Datasheet

Protocols

Questions & Answers

Antibody Testing Data (1)
Published Figures (1)

CD45RB Antibody in Flow Cytometry (Flow)

FIGURE: 1 / 2

CD45RB Antibody (11-0455-82) in Flow

Staining of BALB/c splenocytes with 0.25 µg of Rat IgG2a kappa Isotype Control FITC (Product # 11-4321-42) (open histogram) or 0.25 µg of Anti-Mouse CD45RB FITC (filled histogram). Total viable cells were used for analysis.

Product Image Gallery

Product Details

11-0455-82

Applications

Tested Dilution

Publications

Flow Cytometry (Flow)

0.5 µg/test

View 9 publications 9 publications

Product Specifications

Species Reactivity

Mouse

Published species

Human, Mouse

Host/Isotype

Rat / IgG2a, kappa

Recommended Isotype Control

Rat IgG2a kappa Isotype Control (eBR2a), FITC, eBioscience™

Class

Monoclonal

Type

Antibody

Clone

C363.16A

Conjugate

FITC FITC FITC

Excitation/Emission Max

498/517 nm View spectra

Form

Liquid

Concentration

0.5 mg/mL

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.2

Contains

0.09% sodium azide

Storage conditions

4° C, store in dark, DO NOT FREEZE!

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_465064

Product Specific Information

Description: The C363.16A monoclonal antibody reacts with the exon B-dependent isoform of mouse CD45. This CD45 isoform is expressed by thymocytes, peripheral B cells and a subpopulation of T cells at high density and by dendritic cells and macrophages at low density. CD45 is a protein tyrosine phosphatase and serves an important role in leukocyte signal transduction.

Applications Reported: The C363.16A antibody has been reported for use in flow cytometric analysis.

Applications Tested: The C363.16A antibody has been tested by flow cytometric analysis of mouse splenocytes. This can be used at less than or equal to 0.5 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

Excitation: 488 nm; Emission: 520 nm; Laser: Blue Laser.

Filtration: 0.2 µm post-manufacturing filtered.

Target Information

CD45RB is a receptor-type protein tyrosine phosphatase, CD45 glycoprotein. CD45 is crucial in lymphocyte development and antigen signaling, serving as an important regulator of Src-family kinases, promotes cell survival by modulating integrin-mediated signal transduction pathway and is also involved in DNA fragmentation during apoptosis. CD45 isoforms differ in their extracellular domains, but share identical transmembrane and cytoplasmic domains. CD45 isoforms differ in their ability to translocate into the glycosphingolipid-enriched membrane domains and their expression depends on cell type and physiological state of the cell. CD45RB is expressed in microglia and inflammatory cells. In addition to involvement in immunoreceptor signaling, CD45 is important in promoting cell survival by modulating integrin-mediated signal transduction pathway and is involved in DNA fragmentation during apoptosis. CD45 is expressed on cells of the human hematopoietic lineage with the exception of mature red cells.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

How to use the Panel Builder

Watch the video to learn how to use the Invitrogen Flow Cytometry Panel Builder to build your next flow cytometry panel in 5 easy steps.

Product References

Cytosolic Nuclear Sensor Dhx9 Controls Medullary Thymic Epithelial Cell Differentiation by p53-Mediated Pathways.

Frontiers in immunology

Dong X,Zhang J,Zhang Q,Liang Z,Xu Y,Zhao Y,Zhang B

11-0455-82 was used in Flow cytometry/Cell sorting to reveal that Dhx9, a cytosolic nuclear sensor recognizes viral DNA or RNA, playing an important role in mTEC development and function in mice.

Tue Jun 21 00:00:00 EDT 2022

Butyrophilin-like proteins display combinatorial diversity in selecting and maintaining signature intraepithelial γδ T cell compartments.

Nature communications

Jandke A,Melandri D,Monin L,Ushakov DS,Laing AG,Vantourout P,East P,Nitta T,Narita T,Takayanagi H,Feederle R,Hayday A

11-0455 was used in Flow cytometry/Cell sorting to conclude that Btnl proteins are required to select and to maintain the phenotypes of tissue-protective γδ IEL compartments, with combinatorially diverse heteromers having differential impacts on different IEL subsets.

Tue Jul 28 00:00:00 EDT 2020

Butyrophilin-like proteins display combinatorial diversity in selecting and maintaining signature intraepithelial γδ T cell compartments.

Nature communications

Jandke A,Melandri D,Monin L,Ushakov DS,Laing AG,Vantourout P,East P,Nitta T,Narita T,Takayanagi H,Feederle R,Hayday A

Tue Jul 28 00:00:00 EDT 2020

Effector CD4 T cells with progenitor potential mediate chronic intestinal inflammation.

The Journal of experimental medicine

Shin B,Kress RL,Kramer PA,Darley-Usmar VM,Bellis SL,Harrington LE

11-0455 was used in Flow cytometry/Cell sorting to indicate that the chronicity of T cell-mediated inflammation is perpetuated by specific effector CD4 T cells with stem-like properties.

Mon Jul 02 00:00:00 EDT 2018

IL-12 and IL-4 activate a CD39-dependent intrinsic peripheral tolerance mechanism in CD8(+) T cells.

European journal of immunology

Noble A,Mehta H,Lovell A,Papaioannou E,Fairbanks L

11-0455 was used in Flow cytometry/Cell sorting to study the upregulation of CD39 on CD8(+) T cells and identify a novel pathway of peripheral tolerance.

Wed Jun 01 00:00:00 EDT 2016

Adjuvanted multi-epitope vaccines protect HLA-A*11:01 transgenic mice against Toxoplasma gondii.

JCI insight

El Bissati K,Chentoufi AA,Krishack PA,Zhou Y,Woods S,Dubey JP,Vang L,Lykins J,Broderick KE,Mui E,Suzuki Y,Sa Q,Bi S,Cardona N,Verma SK,Fraczek L,Reardon CA,Sidney J,Alexander J,Sette A,Vedvick T,Fox C,Guderian JA,Reed S,Roberts CW,McLeod R

11-0455 was used in Flow cytometry/Cell sorting to develop multi-epitope DNA or protein vaccines with TLR4 ligand emulsion adjuvant to protect against Toxoplasma gondii.

Thu Sep 22 00:00:00 EDT 2016

STAT4 controls GM-CSF production by both Th1 and Th17 cells during EAE.

Journal of neuroinflammation

McWilliams IL,Rajbhandari R,Nozell S,Benveniste E,Harrington LE

11-0455 was used in Flow cytometry/Cell sorting to establish the role of STAT4 in the regulation of cytokine production during experimental autoimmune encephalomyelitis pathogenesis.

Tue Jun 30 00:00:00 EDT 2015

Leishmania braziliensis infection induces dendritic cell activation, ISG15 transcription, and the generation of protective immune responses.

Journal of immunology (Baltimore, Md. : 1950)

Vargas-Inchaustegui DA,Xin L,Soong L

11-0455 was used in Flow cytometry/Cell sorting to investigate whether Leishmania braziliensis infection efficiently triggers innate responses in dendritic cells and the subsequent adaptive immune responses for parasite clearance.

Sun Jun 01 00:00:00 EDT 2008

Effects of CXCL10 on dendritic cell and CD4+ T-cell functions during Leishmania amazonensis infection.

Infection and immunity

Vasquez RE,Xin L,Soong L

11-0455 was used in Flow cytometry/Cell sorting to investigate the mechanism underlying enhanced immunity against L. amazonensis infection following CXC10 treatment, showing that treatment has an effect on dendritic cell and CD4+ T-cell functions.

Tue Jan 01 00:00:00 EST 2008

Role of interleukin-1beta in activating the CD11c(high) CD45RB- dendritic cell subset and priming Leishmania amazonensis-specific CD4+ T cells in vitro and in vivo.

Infection and immunity

Xin L,Li Y,Soong L

11-0455 was used in Flow cytometry/Cell sorting to investigate the mechanism by which the Leishmania amazonensis parasite modulates antigen-presenting cells and disrupts the immune system.

Mon Oct 01 00:00:00 EDT 2007

Bioinformatics

Protein Aliases: CD45; L-CA; Leukocyte common antigen; Ly-5; Lymphocyte antigen 5; lymphocyte common antigen; Receptor-type tyrosine-protein phosphatase C; T200

View more View less

Gene Aliases: B220; Cd45; CD45R; L-CA; loc; Ly-5; Lyt-4; Ptprc; T200

View more View less

UniProt ID: (Mouse) P06800

View more View less

Entrez Gene ID: (Mouse) 19264

View more View less

Function(s)

phosphoprotein phosphatase activity protein tyrosine phosphatase activity protein binding heparin binding hydrolase activity phosphatase activity protein kinase binding heparan sulfate proteoglycan binding protein phosphatase protein modifying enzyme

Process(es)

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

Learn more

CD45RB Monoclonal Antibody (C363.16A), FITC, eBioscience™

CD45RB Antibody (11-0455-82) in Flow

Product Details

11-0455-82

Flow Cytometry (Flow)

Species Reactivity

Published species

Host/Isotype

Recommended Isotype Control

Class

Type

Clone

Conjugate

Excitation/Emission Max

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

How to use the Panel Builder

Bioinformatics

Performance Guarantee

We're here to help

Your items have has been added!