Invitrogen

CD63 Monoclonal Antibody (H5C6), PE, eBioscience™

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Antibody Testing Data (1)
Published Figures (9)

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CD63 Antibody (12-0639-42) in Flow

Staining of normal human peripheral blood cells with Anti-Human CD193 (CCR3) APC (Product # 17-1939-42), Anti-Human IgE FITC (Product # 11-6986-42) and Mouse IgG1 K Isotype Control PE (Product # 12-4714-81) (blue histogram) or Anti-Human CD63 PE (purple histogram). CD193+IgE+ cells (left) were used for analysis.

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Product Details

12-0639-42

Applications

Tested Dilution

Publications

Western Blot (WB)

View 2 publications 2 publications

Flow Cytometry (Flow)

5 µL (0.125 µg)/test

View 12 publications 12 publications

Product Specifications

Species Reactivity

Human

Published species

Human

Host/Isotype

Mouse / IgG1, kappa

Recommended Isotype Control

Mouse IgG1 kappa Isotype Control (P3.6.2.8.1), PE, eBioscience™

Class

Monoclonal

Type

Antibody

Clone

H5C6

Conjugate

PE PE PE

Excitation/Emission Max

565/576 nm View spectra

Form

Liquid

Concentration

5 µL/Test

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.2, with 0.2% BSA

Contains

0.09% sodium azide

Storage conditions

4° C, store in dark, DO NOT FREEZE!

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2572565

Product Specific Information

Description: This H5C6 monoclonal antibody reacts with human CD63, a type III member of the tetraspanin family of transmembrane proteins. CD63 is expressed intracellularly on lysosomes, endosomes, and granules of resting platelets and basophils. However, cell surface expression of CD63 can be detected on activated basophils and platelets, monocytes, macrophages, and granulocytes. This receptor is also expressed on endothelial cells, fibroblasts, and smooth muscle cells. Studies have demonstrated that CD63 associates with integrins (VLA-3 and VLA-6) and TIMP-1 to mediate the allergic response.

Applications Reported: This H5C6 antibody has been reported for use in flow cytometric analysis.

Applications Tested: This H5C6 antibody has been pre-titrated and tested by flow cytometric of normal human peripheral blood cells. This can be used at 5 µL (0.125 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test.

Excitation: 488-561 nm; Emission: 578 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser.

Filtration: 0.2 µm post-manufacturing filtered.

Target Information

CD63 (LAMP-3, lysosome-associated membrane protein-3), a glycoprotein of tetraspanin family, is present in late endosomes, lysosomes and secretory vesicles of various cell types. CD63 is also present in the plasma membrane, usually following cell activation. Hence, CD63 has become a widely used basophil activation marker. In mast cells, however, CD63 exposition does not need their activation. CD63 interacts with integrins and affects phagocytosis and cell migration, it is also involved in H/K-ATPase trafficking regulation of ROMK1 channels. CD63 also serves as a T-cell costimulation molecule. Expression of CD63 can be used for predicting the prognosis in earlier stages of carcinomas. CD63 is expressed on activated platelets, and is a lysosomal membrane glycoprotein that is translocated to plasma membrane after platelet activation. CD63 is also present in monocytes and macrophages and is weakly expressed on granulocytes, B, and T cells. CD63 is identical to the melanoma-associated antigen which is ME491 and to the platelet antigen PTLGP40. Diseases associated with CD63 dysfunction include melanoma and Hermansky-Pudlak Syndrome.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Product References

Stem cell-derived exosomes from human exfoliated deciduous teeth promote angiogenesis in hyperglycemic-induced human umbilical vein endothelial cells.

Journal of applied oral science : revista FOB

Sunartvanichkul T,Arayapisit T,Sangkhamanee SS,Chaweewannakorn C,Iwasaki K,Klaihmon P,Sritanaudomchai H

12-0639-42 was used in Flow cytometry/Cell sorting to investigate the angiogenesis in human umbilical vein endothelial cells (HUVEC) under high glucose concentration, treated with exosomes derived from stem cells from human exfoliated deciduous teeth (SHED).

Fri Apr 14 00:00:00 EDT 2023

Stem cell-derived exosomes from human exfoliated deciduous teeth promote angiogenesis in hyperglycemic-induced human umbilical vein endothelial cells.

Journal of applied oral science : revista FOB

Sunartvanichkul T,Arayapisit T,Sangkhamanee SS,Chaweewannakorn C,Iwasaki K,Klaihmon P,Sritanaudomchai H

Fri Apr 14 00:00:00 EDT 2023

M2-like tumor-associated macrophages transmit exosomal miR-27b-3p and maintain glioblastoma stem-like cell properties.

Cell death discovery

Zhao G,Ding L,Yu H,Wang W,Wang H,Hu Y,Qin L,Deng G,Xie B,Li G,Qi L

Published figure using CD63 monoclonal antibody (Product # 12-0639-42) in Flow Cytometry

Thu Aug 04 00:00:00 EDT 2022

M2-like tumor-associated macrophages transmit exosomal miR-27b-3p and maintain glioblastoma stem-like cell properties.

Cell death discovery

Zhao G,Ding L,Yu H,Wang W,Wang H,Hu Y,Qin L,Deng G,Xie B,Li G,Qi L

Published figure using CD63 monoclonal antibody (Product # 12-0639-42) in Flow Cytometry

Thu Aug 04 00:00:00 EDT 2022

Excess fatty acids induce pancreatic acinar cell pyroptosis through macrophage M1 polarization.

BMC gastroenterology

Xia W,Lu Z,Chen W,Zhou J,Zhao Y

Published figure using CD63 monoclonal antibody (Product # 12-0639-42) in Western Blot

Sat Feb 19 00:00:00 EST 2022

Unsupervised Machine Learning-Based Clustering of Nanosized Fluorescent Extracellular Vesicles.

Small (Weinheim an der Bergstrasse, Germany)

Kuypers S,Smisdom N,Pintelon I,Timmermans JP,Ameloot M,Michiels L,Hendrix J,Hosseinkhani B

12-0639-42 was used in Western Blot to assess both the purity and the inflammatory status of EV, and compare cell culture and plasma-derived EV isolated via different purification methods.

Mon Feb 01 00:00:00 EST 2021

Enrichment of CD44 in Exosomes From Breast Cancer Cells Treated With Doxorubicin Promotes Chemoresistance.

Frontiers in oncology

Wang X,Cheng K,Zhang G,Jia Z,Yu Y,Guo J,Hua Y,Guo F,Li X,Zou W,Sun H,Dong J,Yang Z

12-0639 was used in Flow cytometry/Cell sorting to conclude that breast cancer cells could promote chemoresistance via exosomal transfer of proteins, such as CD44.

Mon Sep 28 00:00:00 EDT 2020

Proteomic and lipidomic analysis of exosomes derived from ovarian cancer cells and ovarian surface epithelial cells.

Journal of ovarian research

Cheng L,Zhang K,Qing Y,Li D,Cui M,Jin P,Xu T

Published figure using CD63 monoclonal antibody (Product # 12-0639-42) in Flow Cytometry

Wed Jan 22 00:00:00 EST 2020

Comprehensive Cell Surface Antigen Analysis Identifies Transferrin Receptor Protein-1 (CD71) as a Negative Selection Marker for Human Neuronal Cells.

Stem cells (Dayton, Ohio)

Menon V,Thomas R,Elgueta C,Horl M,Osborn T,Hallett PJ,Bartos M,Isacson O,Pruszak J

Tue Oct 01 00:00:00 EDT 2019

Ionizing Radiation Increases the Activity of Exosomal Secretory Pathway in MCF-7 Human Breast Cancer Cells: A Possible Way to Communicate Resistance against Radiotherapy.

International journal of molecular sciences

Jabbari N,Nawaz M,Rezaie J

Published figure using CD63 monoclonal antibody (Product # 12-0639-42) in Flow Cytometry

Thu Jul 25 00:00:00 EDT 2019

Human basophils are a source of - and are differentially activated by - IL-31.

Clinical and experimental allergy : journal of the British Society for Allergy and Clinical Immunology

Raap U,Gehring M,Kleiner S,Rüdrich U,Eiz-Vesper B,Haas H,Kapp A,Gibbs BF

12063942 was used in flow cytometry to assess the functional role of IL-31 in basophil biology

Sat Apr 01 00:00:00 EDT 2017

MicroRNA-containing extracellular vesicles released from endothelial colony-forming cells modulate angiogenesis during ischaemic retinopathy.

Journal of cellular and molecular medicine

Dellett M,Brown ED,Guduric-Fuchs J,O'Connor A,Stitt AW,Medina RJ,Simpson DA

12-0639 was used in Flow cytometry/Cell sorting to evaluate the potential of isolated extracellular vesicles to influence endothelial cell function, and act as a therapy to modulate angiogenesis.

Fri Dec 01 00:00:00 EST 2017

MicroRNA-containing extracellular vesicles released from endothelial colony-forming cells modulate angiogenesis during ischaemic retinopathy.

Journal of cellular and molecular medicine

Dellett M,Brown ED,Guduric-Fuchs J,O'Connor A,Stitt AW,Medina RJ,Simpson DA

12-0639 was used in Flow cytometry/Cell sorting to evaluate the potential of isolated extracellular vesicles to influence endothelial cell function, and act as a therapy to modulate angiogenesis.

Fri Dec 01 00:00:00 EST 2017

Urinary exosomes as a novel biomarker for evaluation of α-lipoic acid's protective effect in early diabetic nephropathy.

Journal of clinical laboratory analysis

Sun H,Yao W,Tang Y,Zhuang W,Wu D,Huang S,Sheng H

Published figure using CD63 monoclonal antibody (Product # 12-0639-42) in Flow Cytometry

Wed Nov 01 00:00:00 EDT 2017

Pathological manifestations in lymphatic filariasis correlate with lack of inhibitory properties of IgG4 antibodies on IgE-activated granulocytes.

PLoS neglected tropical diseases

Prodjinotho UF,von Horn C,Debrah AY,Batsa Debrah L,Albers A,Layland LE,Hoerauf A,Adjobimey T

12-0639 was used in Flow cytometry/Cell sorting to suggest that alterations in IgG4 antibodies are associated with the different clinical phenotypes in lymphatic filariasis endemic regions.

Sat Jul 01 00:00:00 EDT 2017

The regulatory effect of UL-16 binding protein-3 expression on the cytotoxicity of NK cells in cancer patients.

Scientific reports

Mou X,Zhou Y,Jiang P,Zhou T,Jiang Q,Xu C,Liu H,Zheng T,Yuan G,Zhang Y,Chen D,Mao C

12-0639 was used in Flow cytometry/Cell sorting to demonstrate that tumor cells express surface and soluble ULBP3, which regulate NK cell activity.

Wed Aug 20 00:00:00 EDT 2014

Microvesicles derived from human umbilical cord Wharton's jelly mesenchymal stem cells attenuate bladder tumor cell growth in vitro and in vivo.

PloS one

Wu S,Ju GQ,Du T,Zhu YJ,Liu GH

Published figure using CD63 monoclonal antibody (Product # 12-0639-42) in Flow Cytometry

Tue Oct 22 00:00:00 EDT 2013

Bioinformatics

Protein Aliases: CD 63; CD63; CD63 antigen; CD63 antigen (melanoma 1 antigen); Granulophysin; LAMP-3; Limp1; Lysosomal-associated membrane protein 3; Lysosome integral membrane protein 1; lysosome-associated membrane glycoprotein 3; Melanoma-associated antigen ME491; melanoma-associated antigen MLA1; Ocular melanoma-associated antigen; OMA81H; Tetraspanin-30; Tspan-30

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Gene Aliases: CD63; LAMP-3; ME491; MLA1; OMA81H; TSPAN30

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UniProt ID: (Human) P08962

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Entrez Gene ID: (Human) 967

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Function(s)

protein binding protein complex binding

Process(es)

positive regulation of receptor internalization platelet degranulation cell-matrix adhesion negative regulation of epithelial cell migration protein transport cell migration epithelial cell differentiation cellular protein localization endosome to melanosome transport positive regulation of cell adhesion pigment granule maturation regulation of vascular endothelial growth factor signaling pathway regulation of rubidium ion transport positive regulation of integrin-mediated signaling pathway

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CD63 Monoclonal Antibody (H5C6), PE, eBioscience™

CD63 Antibody (12-0639-42) in Flow

Product Details

12-0639-42

Western Blot (WB)

Flow Cytometry (Flow)

Species Reactivity

Published species

Host/Isotype

Recommended Isotype Control

Class

Type

Clone

Conjugate

Excitation/Emission Max

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

How to use the Panel Builder

Bioinformatics

Performance Guarantee

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