Invitrogen

CD90.2 (Thy-1.2) Monoclonal Antibody (53-2.1), PE, eBioscience™

CD90.2 (Thy-1.2) Antibody in Flow Cytometry (Flow)

FIGURE: 1 / 16

CD90.2 (Thy-1.2) Antibody (12-0902-82) in Flow

Staining of mouse splenocytes with Rat IgG2a kappa Isotype Control FITC (Product # 11-4321-42) (left, open histogram) or Rat IgG2a kappa Isotype Control PE (Product # 12-4321-80) (right, open histogram) and Anti-Mouse CD90.2 (Thy-1.2) FITC (Product # 11-0903-82) (left, filled histogram) or Anti-Mouse CD90.2 (Thy-1.2) PE (right, filled histogram). Total viable cells were used for analysis.

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Product Details

12-0902-82

Applications

Tested Dilution

Publications

Immunohistochemistry (Frozen) (IHC (F))

View 1 publication 1 publication

Flow Cytometry (Flow)

0.03 µg/test

View 27 publications 27 publications

Miscellaneous PubMed (Misc)

View 1 publication 1 publication

Product Specifications

Species Reactivity

Mouse

Published species

Mouse

Host/Isotype

Rat / IgG2a, kappa

Recommended Isotype Control

Rat IgG2a kappa Isotype Control (eBR2a), PE, eBioscience™

Class

Monoclonal

Type

Antibody

Clone

53-2.1

Conjugate

PE PE PE

Excitation/Emission Max

565/576 nm View spectra

Form

Liquid

Concentration

0.2 mg/mL

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.2

Contains

0.09% sodium azide

Storage conditions

4° C, store in dark, DO NOT FREEZE!

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_465776

Product Specific Information

Description: The 53-2.1 monoclonal antibody reacts with mouse CD90.2 also known as Thy-1.2, a GPI-linked membrane molecule. CD90.2 is expressed by mouse thymocytes and mature T cells as well as neurons in CD90.2-expressing mouse strains. These strains include BALB/c, CBA, C3H, C57BL/6, C58/, SJL and others. Cells from CD90.1-expressing strains including PL and AKR do not stain with 53-2.1. CD90 is involved in regulation of adhesion and signal transduction by T cells.

Applications Reported: The 53-2.1 antibody has been reported for use in flow cytometric analysis.

Applications Tested: The 53-2.1 antibody has been tested by flow cytometric analysis of mouse splenocytes. This can be used at less than or equal to 0.03 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

Excitation: 488-561 nm; Emission: 578 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser.

Filtration: 0.2 µm post-manufacturing filtered.

Target Information

CD90.2 (Thy 1) antigen is a GPI linked glycoprotein member of the Immunoglobulin super family. CD90.2 is expressed in murine T cells, thymocytes, neural cells, Kupffer's cells and fibroblasts. Thy 1 may play a role in cell-cell or cell-ligand interactions during synaptogenesis and other events in the brain. CD90.2 is an 18 kDa plasma membrane antigen expressed by hematopoietic cells and neurons in all species studied, highly expressed in connective tissue, various fibroblast and stromal cell lines and lastly, by tumor endothelial cell lines. In the mouse, CD90.2 is expressed on all thymocytes and peripheral T lymphocytes.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Product References

A single-cell transcriptional atlas reveals resident progenitor cell niche functions in TMJ disc development and injury.

Nature communications

Bi R,Yin Q,Li H,Yang X,Wang Y,Li Q,Fang H,Li P,Lyu P,Fan Y,Ying B,Zhu S

Published figure using CD90.2 (Thy-1.2) monoclonal antibody (Product # 12-0902-82) in Flow Cytometry

Tue Feb 14 00:00:00 EST 2023

Up-regulation of CREB-1 regulates tendon adhesion in the injury tendon healing through the CREB-1/TGF-β3 signaling pathway.

BMC musculoskeletal disorders

Wu LM,Wang YJ,Li SF,Wang JK,Liu J,Fan CC,Xiong Y

12-0902-82 was used in Flow cytometry/Cell sorting to explore the mechanism of the healing of tendon tissue and anti-adhesion, and to discuss the role of the transforming growth factor-β3 (TGF-β3)/cAMP response element binding protein-1 (CREB-1) signaling pathway in the healing process of tendons.

Tue Apr 25 00:00:00 EDT 2023

Therapeutic Potential of Mesenchymal Stem Cells in a Pre-Clinical Model of Diabetic Kidney Disease and Obesity.

International journal of molecular sciences

Sávio-Silva C,Soinski-Sousa PE,Simplício-Filho A,Bastos RMC,Beyerstedt S,Rangel ÉB

Published figure using CD90.2 (Thy-1.2) monoclonal antibody (Product # 12-0902-82) in Flow Cytometry

Thu Feb 04 00:00:00 EST 2021

Extracellular HMGB-1 activates inflammatory signaling in tendon cells and tissues.

Therapeutic advances in chronic disease

Zhang C,Gu X,Zhao G,Wang W,Shao J,Zhu J,Yuan T,Sun J,Nie D,Zhou Y

Published figure using CD90.2 (Thy-1.2) monoclonal antibody (Product # 12-0902-82) in Flow Cytometry

Sun Apr 17 00:00:00 EDT 2022

Loss of Fas signaling in fibroblasts impairs homeostatic fibrosis resolution and promotes persistent pulmonary fibrosis.

JCI insight

Redente EF,Chakraborty S,Sajuthi S,Black BP,Edelman BL,Seibold MA,Riches DW

Published figure using CD90.2 (Thy-1.2) monoclonal antibody (Product # 12-0902-82) in Flow Cytometry

Tue Dec 08 00:00:00 EST 2020

Cardiac fibroblast proliferation rates and collagen expression mature early and are unaltered with advancing age.

JCI insight

Wu R,Ma F,Tosevska A,Farrell C,Pellegrini M,Deb A

Published figure using CD90.2 (Thy-1.2) monoclonal antibody (Product # 12-0902-82) in Flow Cytometry

Thu Dec 17 00:00:00 EST 2020

Loss of Arid1a Promotes Neuronal Survival Following Optic Nerve Injury.

Frontiers in cellular neuroscience

Peng XQ,Dai SK,Li CP,Liu PP,Wang ZM,Du HZ,Teng ZQ,Yang SG,Liu CM

12-0902 was used in Flow cytometry/Cell sorting to show that optic nerve injury induces dynamic changes of Arid1a expression.

Mon Sep 28 00:00:00 EDT 2020

Knocking Out Non-muscle Myosin II in Retinal Ganglion Cells Promotes Long-Distance Optic Nerve Regeneration.

Cell reports

Wang XW,Yang SG,Zhang C,Hu MW,Qian J,Ma JJ,Zhang Y,Yang BB,Weng YL,Ming GL,Kosanam AR,Zhou FQ

12-0902 was used in Flow cytometry/Cell sorting to demonstrate that knocking out myosin IIA and IIB (myosin IIA/B) in retinal ganglion cells alone, either before or after optic nerve crush, induces significant optic nerve regeneration.

Tue Apr 21 00:00:00 EDT 2020

Thymic Development of a Unique Bone Marrow-Resident Innate-like T Cell Subset with a Potent Innate Immune Function.

Journal of immunology (Baltimore, Md. : 1950)

Yamamoto R,Xu Y,Ikeda S,Sumida K,Tanaka H,Hozumi K,Takaori-Kondo A,Minato N

12-0902-82 was used in Flow Cytometry to suggest that αβT lineage cells that are developed in the thymus by default of TCR-mediated selection are maintained and differentiated to innate-like T cells in BM and may play a role in innate immunity in the hematopoietic environment.

Mon Jul 01 00:00:00 EDT 2019

Mutations in 5-methylcytosine oxidase TET2 and RhoA cooperatively disrupt T cell homeostasis.

The Journal of clinical investigation

Zang S,Li J,Yang H,Zeng H,Han W,Zhang J,Lee M,Moczygemba M,Isgandarova S,Yang Y,Zhou Y,Rao A,You MJ,Sun D,Huang Y

Published figure using CD90.2 (Thy-1.2) monoclonal antibody (Product # 12-0902-82) in Flow Cytometry

Tue Aug 01 00:00:00 EDT 2017

Lymphatic Contribution to the Cellular Niche in Heterotopic Ossification.

Annals of surgery

Loder S,Agarwal S,Sorkin M,Breuler C,Li J,Peterson J,Gardenier J,Hsieh HH,Wang SC,Mehrara BJ,Levi B

Published figure using CD90.2 (Thy-1.2) monoclonal antibody (Product # 12-0902-82) in Flow Cytometry

Thu Dec 01 00:00:00 EST 2016

Injectable, spontaneously assembling, inorganic scaffolds modulate immune cells in vivo and increase vaccine efficacy.

Nature biotechnology

Kim J,Li WA,Choi Y,Lewin SA,Verbeke CS,Dranoff G,Mooney DJ

Published figure using CD90.2 (Thy-1.2) monoclonal antibody (Product # 12-0902-82) in Flow Cytometry

Thu Jan 01 00:00:00 EST 2015

Disruption of p21-activated kinase 1 gene diminishes atherosclerosis in apolipoprotein E-deficient mice.

Nature communications

Singh NK,Kotla S,Dyukova E,Traylor JG,Orr AW,Chernoff J,Marion TN,Rao GN

12-0902 was used in Flow cytometry/Cell sorting to investigate the role of Pak1 in atherogenesis.

Wed Jun 24 00:00:00 EDT 2015

Induction of immunomodulatory monocytes by human mesenchymal stem cell-derived hepatocyte growth factor through ERK1/2.

Journal of leukocyte biology

Chen PM,Liu KJ,Hsu PJ,Wei CF,Bai CH,Ho LJ,Sytwu HK,Yen BL

12-0902-82 was used in Magnetic cell separation to study the pleomorphic nature of mesenchymal stem cell immunomodulation, and highlight the important role of immunomodulatory monocytes in altering T cell effector function.

Fri Aug 01 00:00:00 EDT 2014

Twist2 promotes self-renewal of liver cancer stem-like cells by regulating CD24.

Carcinogenesis

Liu AY,Cai Y,Mao Y,Lin Y,Zheng H,Wu T,Huang Y,Fang X,Lin S,Feng Q,Huang Z,Yang T,Luo Q,Ouyang G

12-0902 was used in Flow cytometry/Cell sorting to demonstrate that Twist2 promotes self-renewal in liver cancer stem-like cells by regulating CD24.

Sat Mar 01 00:00:00 EST 2014

Adoptive transfer of cytotoxic T lymphocytes targeting two different antigens limits antigen loss and tumor escape.

Human gene therapy

Kaluza KM,Kottke T,Diaz RM,Rommelfanger D,Thompson J,Vile R

12-0902 was used in Flow cytometry/Cell sorting to study whether cytotoxic T lymphocytes targeting multiple antigens are more effective for preventing tumour escape than those targeting a single antigen.

Mon Oct 01 00:00:00 EDT 2012

Partial characterization of the Sox2+ cell population in an adult murine model of digit amputation.

Tissue engineering. Part A

Agrawal V,Siu BF,Chao H,Hirschi KK,Raborn E,Johnson SA,Tottey S,Hurley KB,Medberry CJ,Badylak SF

Published figure using CD90.2 (Thy-1.2) monoclonal antibody (Product # 12-0902-82) in Flow Cytometry

Sun Jul 01 00:00:00 EDT 2012

Nuclear export of the NF-κB inhibitor IκBα is required for proper B cell and secondary lymphoid tissue formation.

Immunity

Wuerzberger-Davis SM,Chen Y,Yang DT,Kearns JD,Bates PW,Lynch C,Ladell NC,Yu M,Podd A,Zeng H,Huang TT,Wen R,Hoffmann A,Wang D,Miyamoto S

Published figure using CD90.2 (Thy-1.2) monoclonal antibody (Product # 12-0902-82) in Flow Cytometry

Fri Feb 25 00:00:00 EST 2011

Bone marrow-derived smooth muscle-like cells are infrequent in advanced primary atherosclerotic plaques but promote atherosclerosis.

Arteriosclerosis, thrombosis, and vascular biology

Yu H,Stoneman V,Clarke M,Figg N,Xin HB,Kotlikoff M,Littlewood T,Bennett M

12-0902 was used in Flow cytometry/Cell sorting to discover that bone marrow-derived SMC-like cells are infrequent in advanced primary atherosclerotic plaques and absent in fibrous caps.

Wed Jun 01 00:00:00 EDT 2011

Distinct developmental requirements for isolated lymphoid follicle formation in the small and large intestine: RANKL is essential only in the small intestine.

The American journal of pathology

Knoop KA,Butler BR,Kumar N,Newberry RD,Williams IR

12-0902 was used in Immunofluorescence to study the role of RANKL in the development of cryptopatches and isolated lymphoid follicles within intestinal lymphoid tissues.

Sat Oct 01 00:00:00 EDT 2011

T regulatory cells maintain intestinal homeostasis by suppressing γδ T cells.

Immunity

Park SG,Mathur R,Long M,Hosh N,Hao L,Hayden MS,Ghosh S

12-0902 was used in Flow cytometry/Cell sorting to explore the regulatory processes maintaining a tolerogenic attitude towards enteric commensal bacteria.

Wed Nov 24 00:00:00 EST 2010

c-Myb promotes the survival of CD4+CD8+ double-positive thymocytes through upregulation of Bcl-xL.

Journal of immunology (Baltimore, Md. : 1950)

Yuan J,Crittenden RB,Bender TP

12-0902 was used in Flow cytometry/Cell sorting to elucidate the c-Myb dependent mechanism controlling CD4(+)CD8(+) double positive thymocyte survival during maturation.

Mon Mar 15 00:00:00 EDT 2010

Immortalization eliminates a roadblock during cellular reprogramming into iPS cells.

Nature

Utikal J,Polo JM,Stadtfeld M,Maherali N,Kulalert W,Walsh RM,Khalil A,Rheinwald JG,Hochedlinger K

12-0902 was used in Flow cytometry/Cell sorting to investigate the rare events that allow generation of induced pluripotent stem cells.

Thu Aug 27 00:00:00 EDT 2009

A critical role of TAK1 in B-cell receptor-mediated nuclear factor kappaB activation.

Blood

Schuman J,Chen Y,Podd A,Yu M,Liu HH,Wen R,Chen ZJ,Wang D

Published figure using CD90.2 (Thy-1.2) monoclonal antibody (Product # 12-0902-82) in Flow Cytometry

Thu May 07 00:00:00 EDT 2009

Tracking epitope-specific T cells.

Nature protocols

Moon JJ,Chu HH,Hataye J,Pagán AJ,Pepper M,McLachlan JB,Zell T,Jenkins MK

Published figure using CD90.2 (Thy-1.2) monoclonal antibody (Product # 12-0902-82) in Flow Cytometry

Tue Jun 30 00:00:00 EDT 2009

Manganese superoxide dismutase gene dosage affects chromosomal instability and tumor onset in a mouse model of T cell lymphoma.

Free radical biology & medicine

van de Wetering CI,Coleman MC,Spitz DR,Smith BJ,Knudson CM

Published figure using CD90.2 (Thy-1.2) monoclonal antibody (Product # 12-0902-82) in Flow Cytometry

Tue Apr 15 00:00:00 EDT 2008

Homeostasis and effector function of lymphopenia-induced "memory-like" T cells in constitutively T cell-depleted mice.

Journal of immunology (Baltimore, Md. : 1950)

Voehringer D,Liang HE,Locksley RM

12-0902-82 was used in Flow Cytometry to demonstrate that T(ML) cells are not sufficient to mediate an effective immune response in constitutively T cell-depleted mice.

Tue Apr 01 00:00:00 EDT 2008

Converting IL-15 to a superagonist by binding to soluble IL-15R{alpha}.

Proceedings of the National Academy of Sciences of the United States of America

Rubinstein MP,Kovar M,Purton JF,Cho JH,Boyman O,Surh CD,Sprent J

12-0902 was used in Flow cytometry/Cell sorting to study how IL-2 function is markedly inhibited by binding to soluble IL-2Ralpha.

Tue Jun 13 00:00:00 EDT 2006

Biochemical and functional characterization of three activated macrophage populations.

Journal of leukocyte biology

Edwards JP,Zhang X,Frauwirth KA,Mosser DM

12-0902 was used in Flow cytometry/Cell sorting to identify Ca-Mphi, Mphi-II, and AA-Mphi as three populations of macrophages with different biological functions.

Fri Dec 01 00:00:00 EST 2006

Bioinformatics

Protein Aliases: CD90; FLJ33325; Thy 1.2; Thy-1 antigen; Thy-1 membrane glycoprotein

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Gene Aliases: CD90; T25; Thy-1; Thy-1.2; Thy1; Thy1.1; Thy1.2

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UniProt ID: (Mouse) P01831

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Entrez Gene ID: (Mouse) 21838

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Function(s)

GTPase activator activity integrin binding enzyme binding protein kinase binding GPI anchor binding cell adhesion molecule

Process(es)

angiogenesis negative regulation of protein kinase activity cytoskeleton organization single organismal cell-cell adhesion negative regulation of cell migration positive regulation of GTPase activity retinal cone cell development protein autophosphorylation focal adhesion assembly negative regulation of axonogenesis T cell receptor signaling pathway negative regulation of T cell receptor signaling pathway positive regulation of T cell activation positive regulation of release of sequestered calcium ion into cytosol

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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CD90.2 (Thy-1.2) Monoclonal Antibody (53-2.1), PE, eBioscience™

CD90.2 (Thy-1.2) Antibody (12-0902-82) in Flow

Product Details

12-0902-82

Immunohistochemistry (Frozen) (IHC (F))

Flow Cytometry (Flow)

Miscellaneous PubMed (Misc)

Species Reactivity

Published species

Host/Isotype

Recommended Isotype Control

Class

Type

Clone

Conjugate

Excitation/Emission Max

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

How to use the Panel Builder

Bioinformatics

Performance Guarantee

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