Invitrogen

CD133 (Prominin-1) Monoclonal Antibody (TMP4), PE, eBioscience™

Advanced Verification

This Antibody was verified by Knockout to ensure that the antibody binds to the antigen stated.

10 Published Figures

17 References

View all (29) CD133 (Prominin-1) antibodies

Datasheet

Protocols

Questions & Answers

Datasheet

Protocols

Questions & Answers

CD133 (Prominin-1) Antibody in Flow Cytometry (Flow)

FIGURE: 1 / 13

CD133 (Prominin-1) Antibody (12-1338-42) in Flow

Knockout of CD133 was achieved by CRISPR-Cas9 genome editing using LentiArray™ Lentiviral sgRNA (Product # A32042, Assay ID CRISPR924819_LV) and LentiArray Cas9 Lentivirus (Product # A32064). Flow cytometry analysis of CD133 was performed by staining HT-29 CD133 Knock out cells with 0.25 µg Mouse IgG1 kappa Isotype Control (P3.6.2.8.1), PE, eBioscience™ (Product # 12-4714-82, yellow histogram) or 0.25 µg CD... View More

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Product Details

12-1338-42

Applications

Tested Dilution

Publications

Immunohistochemistry (Paraffin) (IHC (P))

View 1 publication 1 publication

Immunocytochemistry (ICC/IF)

View 2 publications 2 publications

Flow Cytometry (Flow)

5 µL (0.25 µg)/test

View 13 publications 13 publications

Miscellaneous PubMed (Misc)

View 1 publication 1 publication

Product Specifications

Species Reactivity

Human

Published species

Human

Host/Isotype

Mouse / IgG1, kappa

Recommended Isotype Control

Mouse IgG1 kappa Isotype Control (P3.6.2.8.1), PE, eBioscience™

Class

Monoclonal

Type

Antibody

Clone

TMP4

Conjugate

PE PE PE

Excitation/Emission Max

565/576 nm View spectra

Form

Liquid

Concentration

5 µL/Test

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.2, with 0.2% BSA

Contains

0.09% sodium azide

Storage conditions

4° C, store in dark, DO NOT FREEZE!

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_1582257

Product Specific Information

Description: The TMP4 monoclonal antibody reacts with human CD133 (Prominin-1), a 120 kDa member of the pentaspan family of proteins, which also includes Prominin-2. Their expression is found within plasma membrane protrusions such as epithelial microvilli. CD133 can exist in a number of alternatively spliced isoforms, and the protein has several N-linked glycosylation sites: the occurrence of both may be tissue-dependent. Human CD133 was first identified as an epitope expressed on CD34+ hematopoietic progenitors. Although the ligand and function of CD133 remain unknown, it has since proven to be very useful as a marker for both stem cells and cancer stem cells. In addition to its expression on hematopoietic precursors, CD133 has been used to identify tumorigenic colon cancer stem cells, brain cancer stem cells, prostate cancer stem cells, in addition to others.

The binding of the TMP4 antibody does not block the binding of another anti-human CD133 antibody, EMK08 (Product # 12-1339) indicating that they recognize distinct epitopes.

Applications Reported: This TMP4 antibody has been reported for use in flow cytometric analysis.

Applications Tested: This TMP4 antibody has been pre-titrated and tested by flow cytometric analysis of normal human peripheral blood cells. This can be used at 5 µL (0.25 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test.

Excitation: 488-561 nm; Emission: 578 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser.

Filtration: 0.2 µm post-manufacturing filtered.

Target Information

The CD133 (AC133) antigen is a 97 kDa glycoprotein, with 5 transmembrane domains, that shows up as a 120 kDa band on protein gels due to glycosylation. CD133 is a cell surface marker expressed by immature hematopoietic stem cells but not mature blood cells. In addition to hematopoietic stem and progenitor cells, CD133 has been found to be a marker for other stem and progenitor cells including neural and embryonic stem cells. CD133 has been shown to be expressed in cancers, including some leukemias and brain tumors, that may be derived from stem cells. Low level expression could also be detected in the kidney, pancreas, placenta, and fetal liver. Mutations in this gene have been shown to result in retinitis pigmentosa and Stargardt disease. Expression of the CD133 gene is also associated with several types of cancer. CD133 is expressed from at least five alternative promoters that are expressed in a tissue-dependent manner, and multiple transcript variants encoding different isoforms have been found.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Product References

Non-canonical Wnt/Ca2+ signaling is essential to promote self-renewal and proliferation in colon cancer stem cells.

Frontiers in oncology

Sarabia-Sánchez MA,Moreno-Londoño AP,Castañeda-Patlán MC,Alvarado-Ortiz E,Martínez-Morales JC,Robles-Flores M

12-1338-42 was used in Flow Cytometry to indicate that both types of ligands activate the Wnt/Ca2+ signaling axis to induce/maintain the self-renewal efficiency of CSCs, demonstrating to be essential for the functions of CSC in colon cancer.

Tue Mar 28 00:00:00 EDT 2023

Human Adult Renal Progenitor Cells Prevent Cisplatin-Nephrotoxicity by Inducing CYP1B1 Overexpression and miR-27b-3p Down-Regulation through Extracellular Vesicles.

Cells

Franzin R,Stasi A,De Palma G,Picerno A,Curci C,Sebastiano S,Campioni M,Cicirelli A,Rizzo A,Di Lorenzo VF,Pontrelli P,Pertosa GB,Castellano G,Gesualdo L,Sallustio F

12-1338-42 was used in Immunohistochemistry (Paraffin) to investigate the molecular mechanisms underlying the protective effect of tARPC on renal epithelium during cisplatin nephrotoxicity.

Sat Jun 17 00:00:00 EDT 2023

Lipogenesis promotes mitochondrial fusion and maintains cancer stemness in human NSCLC.

JCI insight

Liu Z,Lei J,Wu T,Hu W,Zheng M,Wang Y,Song J,Ruan H,Xu L,Ren T,Xu W,Wen Z

12-1338-42 was used in Flow cytometry/Cell sorting to study OPA1hi with mitochondrial fusion as a metabolic feature of human lung CSCs, licensing their stem-like properties.

Wed Mar 22 00:00:00 EDT 2023

Suppression of Cancer Cell Stemness and Drug Resistance via MYC Destabilization by Deubiquitinase USP45 Inhibition with a Natural Small Molecule.

Cancers

Tu X,Li C,Sun W,Tian X,Li Q,Wang S,Ding X,Huang Z

12-1338-42 was used in Flow cytometry/Cell sorting to identify that USP45 can be specifically bound and inhibited by a natural small molecule (α-mangostin), in turn significantly suppressing USP45-induced stemness and drug resistance.

Wed Feb 01 00:00:00 EST 2023

SUL-109 Protects Hematopoietic Stem Cells from Apoptosis Induced by Short-Term Hypothermic Preservation and Maintains Their Engraftment Potential.

Biology of blood and marrow transplantation : journal of the American Society for Blood and Marrow Transplantation

Aerts-Kaya FSF,Visser TP,Pervin B,Mammadova A,Özyüncü Ö,Wagemaker G,Uçkan-Çetinkaya FD

12-1338 was used in Flow cytometry/Cell sorting to show SUL-109 is a promising novel chemical for use as a protective agent during cold storage of human and murine HSCs.

Wed Apr 01 00:00:00 EDT 2020

Human umbilical cord-derived mesenchymal stem cells and human cord blood mononuclear cells protect against cisplatin-induced acute kidney injury in rat models.

Experimental and therapeutic medicine

Xu Q,Yan P,Duan XJ,Wu X,Chen XJ,Luo M,Peng JC,Feng LX,Liu J,Zhong HL,Cheng W,Zou QY,Duan SB

12-1338-42 was used in Flow Cytometry to determine the protective effects of hUCMSCs and hCBMNCs transplantation therapies on an established CP-induced rat model and explore their underlying mechanisms of action.

Tue Dec 01 00:00:00 EST 2020

The Deubiquitinase USP4 Stabilizes Twist1 Protein to Promote Lung Cancer Cell Stemness.

Cancers

Li F,Hu Q,He T,Xu J,Yi Y,Xie S,Ding L,Fu M,Guo R,Xiao ZJ,Niu M

12-1338 was used in Flow cytometry/Cell sorting to demonstrate that the deubiquitinase USP4 is critically important in promoting lung cancer stemness.

Mon Jun 15 00:00:00 EDT 2020

The Deubiquitinase USP4 Stabilizes Twist1 Protein to Promote Lung Cancer Cell Stemness.

Cancers

Li F,Hu Q,He T,Xu J,Yi Y,Xie S,Ding L,Fu M,Guo R,Xiao ZJ,Niu M

12-1338 was used in Flow cytometry/Cell sorting to demonstrate that the deubiquitinase USP4 is critically important in promoting lung cancer stemness.

Mon Jun 15 00:00:00 EDT 2020

Chemerin enhances the adhesion and migration of human endothelial progenitor cells and increases lipid accumulation in mice with atherosclerosis.

Lipids in health and disease

Jia J,Yu F,Xiong Y,Wei W,Ma H,Nisi F,Song X,Yang L,Wang D,Yuan G,Zhou H

12-1338 was used in Flow cytometry/Cell sorting to explore the effects of chemerin on the functions of human endothelial progenitor cells and to investigate its role in lipid accumulation in ApoE-knockout mice.

Sun Sep 20 00:00:00 EDT 2020

Chemerin enhances the adhesion and migration of human endothelial progenitor cells and increases lipid accumulation in mice with atherosclerosis.

Lipids in health and disease

Jia J,Yu F,Xiong Y,Wei W,Ma H,Nisi F,Song X,Yang L,Wang D,Yuan G,Zhou H

Sun Sep 20 00:00:00 EDT 2020

Profiling and Targeting of Energy and Redox Metabolism in Grade 2 Bladder Cancer Cells with Different Invasiveness Properties.

Cells

Pasquale V,Ducci G,Campioni G,Ventrici A,Assalini C,Busti S,Vanoni M,Vago R,Sacco E

Published figure using CD133 (Prominin-1) monoclonal antibody (Product # 12-1338-42) in Flow Cytometry

Fri Dec 11 00:00:00 EST 2020

Profiling and Targeting of Energy and Redox Metabolism in Grade 2 Bladder Cancer Cells with Different Invasiveness Properties.

Cells

Pasquale V,Ducci G,Campioni G,Ventrici A,Assalini C,Busti S,Vanoni M,Vago R,Sacco E

Published figure using CD133 (Prominin-1) monoclonal antibody (Product # 12-1338-42) in Flow Cytometry

Fri Dec 11 00:00:00 EST 2020

ING5 activity in self-renewal of glioblastoma stem cells via calcium and follicle stimulating hormone pathways.

Oncogene

Wang F,Wang AY,Chesnelong C,Yang Y,Nabbi A,Thalappilly S,Alekseev V,Riabowol K

Published figure using CD133 (Prominin-1) monoclonal antibody (Product # 12-1338-42) in Flow Cytometry

Thu Jan 18 00:00:00 EST 2018

Inhibition of Fas associated phosphatase 1 (Fap1) facilitates apoptosis of colon cancer stem cells and enhances the effects of oxaliplatin.

Oncotarget

Huang W,Bei L,Eklund EA

12-1338 was used in Flow cytometry/Cell sorting to study the effects of inhibiting Fas associated phosphatase 1 in colon cancer stem cells.

Fri May 25 00:00:00 EDT 2018

Modified Leukocyte Filter Removes Tumor Cells from the Salvaged Blood.

PloS one

Mei K,Du L,Yan M,Zhang Z,Zhang F,Gong L,Sun K,Zhang J,Tang Y,Jiang C,Liu J

12-1338 was used in Immunocytochemistry to evaluate the application of modified leukocyte depletion filter for once-surgery, and suggest the potential clinical application for blood cancers treatment.

Mon Mar 21 00:00:00 EDT 2016

Evaluation of cancer stem cell markers CD133, CD44, CD24: association with AKT isoforms and radiation resistance in colon cancer cells.

PloS one

Sahlberg SH,Spiegelberg D,Glimelius B,Stenerlöw B,Nestor M

Fri Jan 09 00:00:00 EST 2015

Comparative evaluation of differentiation potential of menstrual blood- versus bone marrow-derived stem cells into hepatocyte-like cells.

PloS one

Khanjani S,Khanmohammadi M,Zarnani AH,Akhondi MM,Ahani A,Ghaempanah Z,Naderi MM,Eghtesad S,Kazemnejad S

Sat Nov 08 00:00:00 EST 2014

Comparative evaluation of differentiation potential of menstrual blood- versus bone marrow-derived stem cells into hepatocyte-like cells.

PloS one

Khanjani S,Khanmohammadi M,Zarnani AH,Akhondi MM,Ahani A,Ghaempanah Z,Naderi MM,Eghtesad S,Kazemnejad S

Sat Nov 08 00:00:00 EST 2014

Comparative evaluation of differentiation potential of menstrual blood- versus bone marrow-derived stem cells into hepatocyte-like cells.

PloS one

Khanjani S,Khanmohammadi M,Zarnani AH,Akhondi MM,Ahani A,Ghaempanah Z,Naderi MM,Eghtesad S,Kazemnejad S

Sat Nov 08 00:00:00 EST 2014

Established breast cancer stem cell markers do not correlate with in vivo tumorigenicity of tumor-initiating cells.

International journal of oncology

Lehmann C,Jobs G,Thomas M,Burtscher H,Kubbies M

12-1338 was used in Flow cytometry/Cell sorting to determine that established markers of breast cancer stem cells are not indicative of the in vivo tumourigenicity of putative breast tumour-initiating cells.

Sat Dec 01 00:00:00 EST 2012

Bioinformatics

Protein Aliases: Antigen AC133; antigen CD133; CD133; hematopoietic stem cell antigen; hProminin; Lamp II; Lamp-2a; Lamp-2b; Lamp-2c; Lamp2; Mac3; Prominin-1; Prominin-like protein 1; Prominin1

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Gene Aliases: AC133; CD133; CORD12; MCDR2; MSTP061; PROM1; PROML1; RP41; STGD4

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UniProt ID: (Human) Q6SV49

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Entrez Gene ID: (Human) 8842

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Function(s)

protein binding actinin binding cadherin binding

Process(es)

retina layer formation photoreceptor cell maintenance retina morphogenesis in camera-type eye camera-type eye photoreceptor cell differentiation glomerular visceral epithelial cell differentiation glomerular parietal epithelial cell differentiation positive regulation of nephron tubule epithelial cell differentiation

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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CD133 (Prominin-1) Monoclonal Antibody (TMP4), PE, eBioscience™

CD133 (Prominin-1) Antibody (12-1338-42) in Flow

Product Details

12-1338-42

Immunohistochemistry (Paraffin) (IHC (P))

Immunocytochemistry (ICC/IF)

Flow Cytometry (Flow)

Miscellaneous PubMed (Misc)

Species Reactivity

Published species

Host/Isotype

Recommended Isotype Control

Class

Type

Clone

Conjugate

Excitation/Emission Max

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

How to use the Panel Builder

Bioinformatics

Performance Guarantee

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