Invitrogen

CD133 (Prominin-1) Monoclonal Antibody (TMP4), APC, eBioscience™

10 Published Figures

23 References

View all (29) CD133 (Prominin-1) antibodies

Datasheet

Protocols

Questions & Answers

Datasheet

Protocols

Questions & Answers

Antibody Testing Data (1)
Published Figures (10)

CD133 (Prominin-1) Antibody in Flow Cytometry (Flow)

FIGURE: 1 / 11

CD133 (Prominin-1) Antibody (17-1338-42) in Flow

Staining of normal human peripheral blood cells with Anti-Human CD45 PE (Product # 12-9459-42) and Mouse IgG1 K Isotype Control APC (Product # 17-4714-81) (left) or Anti-Human CD133 APC (right). Cells in the lymphocyte gate were used for analysis.

Product Image Gallery

Product Details

17-1338-42

Applications

Tested Dilution

Publications

Flow Cytometry (Flow)

5 µL (0.125 µg)/test

View 23 publications 23 publications

Product Specifications

Species Reactivity

Human

Published species

Human, Mouse

Host/Isotype

Mouse / IgG1, kappa

Recommended Isotype Control

Mouse IgG1 kappa Isotype Control (P3.6.2.8.1), APC, eBioscience™

Class

Monoclonal

Type

Antibody

Clone

TMP4

Conjugate

APC APC APC

Excitation/Emission Max

651/660 nm View spectra

Form

Liquid

Concentration

5 µL/Test

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.2, with 0.2% BSA

Contains

0.09% sodium azide

Storage conditions

4° C, store in dark, DO NOT FREEZE!

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_1603199

Product Specific Information

Description: The TMP4 monoclonal antibody reacts with human CD133 (Prominin-1), a 120 kDa member of the pentaspan family of proteins, which also includes Prominin-2. Their expression is found within plasma membrane protrusions such as epithelial microvilli. CD133 can exist in a number of alternatively spliced isoforms, and the protein has several N-linked glycosylation sites: the occurrence of both may be tissue-dependent. Human CD133 was first identified as an epitope expressed on CD34+ hematopoietic progenitors. Although the ligand and function of CD133 remain unknown, it has since proven to be very useful as a marker for both stem cells and cancer stem cells. In addition to its expression on hematopoietic precursors, CD133 has been used to identify tumorigenic colon cancer stem cells, brain cancer stem cells, prostate cancer stem cells, in addition to others.

The binding of the TMP4 antibody does not block the binding of another anti-human CD133 antibody, EMK08 (Product # 12-1339) indicating that they recognize distinct epitopes.

Applications Reported: This TMP4 antibody has been reported for use in flow cytometric analysis.

Applications Tested: This TMP4 antibody has been pre-titrated and tested by flow cytometric analysis of normal human peripheral blood cells. This can be used at 5 µL (0.125 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test.

Excitation: 633-647 nm; Emission: 660 nm; Laser: Red Laser.

Filtration: 0.2 µm post-manufacturing filtered.

Target Information

The CD133 (AC133) antigen is a 97 kDa glycoprotein, with 5 transmembrane domains, that shows up as a 120 kDa band on protein gels due to glycosylation. CD133 is a cell surface marker expressed by immature hematopoietic stem cells but not mature blood cells. In addition to hematopoietic stem and progenitor cells, CD133 has been found to be a marker for other stem and progenitor cells including neural and embryonic stem cells. CD133 has been shown to be expressed in cancers, including some leukemias and brain tumors, that may be derived from stem cells. Low level expression could also be detected in the kidney, pancreas, placenta, and fetal liver. Mutations in this gene have been shown to result in retinitis pigmentosa and Stargardt disease. Expression of the CD133 gene is also associated with several types of cancer. CD133 is expressed from at least five alternative promoters that are expressed in a tissue-dependent manner, and multiple transcript variants encoding different isoforms have been found.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

How to use the Panel Builder

Watch the video to learn how to use the Invitrogen Flow Cytometry Panel Builder to build your next flow cytometry panel in 5 easy steps.

Product References

HERC2 promotes inflammation-driven cancer stemness and immune evasion in hepatocellular carcinoma by activating STAT3 pathway.

Journal of experimental & clinical cancer research : CR

Liu Y,Xu Q,Deng F,Zheng Z,Luo J,Wang P,Zhou J,Lu X,Zhang L,Chen Z,Zhang Q,Chen Q,Zuo D

17-1338-42 was used in Flow cytometry/Cell sorting to identify HERC2 functions as a previously unknown modulator of the JAK2/STAT3 pathway, thereby promoting inflammation-induced stemness and immune evasion in HCC.

Wed Feb 01 00:00:00 EST 2023

BML-260 promotes the growth of cord blood and mobilized peripheral blood hematopoietic stem and progenitor cells with improved reconstitution ability.

Journal of cellular biochemistry

Albayrak E,Akgol S,Turan RD,Uslu M,Kocabas F

17-1338-42 was used in Flow cytometry/Cell sorting to show that BML-260 molecules can be used for human HSC expansion and regulation of function.

Thu Dec 01 00:00:00 EST 2022

Circ_RNF13 Regulates the Stemness and Chemosensitivity of Colorectal Cancer by Transcriptional Regulation of DDX27 Mediated by TRIM24 Stabilization.

Cancers

Guo Y,Hu G,Tian B,Ma M,Long F,Chen M

17-1338-42 was used in Flow cytometry/Cell sorting to characterize circ_RNF13 and investigate its role in regulating stemness and chemosensitivity in colorectal cancer (CRC), revealing that circ_RNF13 modulates CRC stemness by regulating DDX27 expression through TRIM24-mediated transcriptional regulation and stabilizing TRIM24 by suppressing FBXW7-mediated TRIM24 degradation.

Fri Dec 16 00:00:00 EST 2022

Astragaloside IV - mediated endothelial progenitor cell exosomes promote autophagy and inhibit apoptosis in hyperglycemic damaged endothelial cells via miR-21/PTEN axis.

Folia histochemica et cytobiologica

Xiong W,Huang XL,Li CY,Wang XL,Lan HW,Wang TT,Chen ZL,Yang QP,Hu AL,Xia YF,Zhu FR,Zhou ZZ

17-1338-42 was used in Flow cytometry/Cell sorting to demonstrate that AS-IV-mediated EPC-exos-miR-21 enhances autophagy and depresses apoptosis in HG-damaged endothelial cells via the miR-21/PTEN axis.

Mon Jan 02 00:00:00 EST 2023

Small molecule-mediated modulation of ubiquitination and neddylation improves HSC function ex vivo.

Journal of cellular physiology

Albayrak E,Uslu M,Akgol S,Tuysuz EC,Kocabas F

17-1338-42 was used in flow cytometry to shed light on the contribution of SKP2, and associated ubiquitination and neddylation in HSC maintenance, self-renewal, and expansion.

Wed Dec 01 00:00:00 EST 2021

EMP3 negatively modulates breast cancer cell DNA replication, DNA damage repair, and stem-like properties.

Cell death & disease

Zhou K,Sun Y,Dong D,Zhao C,Wang W

17-1338-42 was used in Flow cytometry/Cell sorting to indicate that EMP3 is a putative tumor suppressor in breast cancer, and EMP3 downregulation may be responsible for breast cancer chemoresistance.

Sun Sep 12 00:00:00 EDT 2021

IGF2BP2 regulates DANCR by serving as an N6-methyladenosine reader.

Cell death and differentiation

Hu X,Peng WX,Zhou H,Jiang J,Zhou X,Huang D,Mo YY,Yang L

17-1338-42 was used in Flow Cytometry to promote cancer stemness-like properties and pancreatic cancer pathogenesis.

Mon Jun 01 00:00:00 EDT 2020

PIM protein kinases regulate the level of the long noncoding RNA H19 to control stem cell gene transcription and modulate tumor growth.

Molecular oncology

Singh N,Padi SKR,Bearss JJ,Pandey R,Okumura K,Beltran H,Song JH,Kraft AS,Olive V

17-1338 was used in Flow cytometry/Cell sorting to study the mechanisms through which PIM kinases regulate tumor growth.

Fri May 01 00:00:00 EDT 2020

Curcumin Enhances Radiosensitization of Nasopharyngeal Carcinoma via Mediating Regulation of Tumor Stem-like Cells by a CircRNA Network.

Journal of Cancer

Zhu D,Shao M,Yang J,Fang M,Liu S,Lou D,Gao R,Liu Y,Li A,Lv Y,Mo Z,Fan Q

17-1338-42 was used in Flow Cytometry to explore the potential relationships among curcumin, circRNAs, and nasopharyngeal carcinoma (NPC).

Mon Sep 28 00:00:00 EDT 2020

High expression of endothelial progenitor cell-induced angiogenic markers is associated with bile acid levels in HCC.

Oncology letters

Chen JL,Wang L,Li R,Jiao YF,Yu WF

17-1338 was used in Flow cytometry/Cell sorting to investigate the factors influencing the number of EPCs and circulating progenitor cells (CPCs), as well as the expression levels of vascular endothelial growth factor receptor 2 (VEGFR-2) and CD34, in patients with hepatocellular carcinoma.

Tue Sep 01 00:00:00 EDT 2020

The Deubiquitinase USP4 Stabilizes Twist1 Protein to Promote Lung Cancer Cell Stemness.

Cancers

Li F,Hu Q,He T,Xu J,Yi Y,Xie S,Ding L,Fu M,Guo R,Xiao ZJ,Niu M

Published figure using CD133 (Prominin-1) monoclonal antibody (Product # 17-1338-42) in Flow Cytometry

Mon Jun 15 00:00:00 EDT 2020

Chemerin enhances the adhesion and migration of human endothelial progenitor cells and increases lipid accumulation in mice with atherosclerosis.

Lipids in health and disease

Jia J,Yu F,Xiong Y,Wei W,Ma H,Nisi F,Song X,Yang L,Wang D,Yuan G,Zhou H

Published figure using CD133 (Prominin-1) monoclonal antibody (Product # 17-1338-42) in Flow Cytometry

Sun Sep 20 00:00:00 EDT 2020

Profiling and Targeting of Energy and Redox Metabolism in Grade 2 Bladder Cancer Cells with Different Invasiveness Properties.

Cells

Pasquale V,Ducci G,Campioni G,Ventrici A,Assalini C,Busti S,Vanoni M,Vago R,Sacco E

Published figure using CD133 (Prominin-1) monoclonal antibody (Product # 17-1338-42) in Flow Cytometry

Fri Dec 11 00:00:00 EST 2020

FZD5 contributes to TNBC proliferation, DNA damage repair and stemness.

Cell death & disease

Sun Y,Wang Z,Na L,Dong D,Wang W,Zhao C

17-1338-42 was used in Flow Cytometry to show that FZD5 is a novel target for the development of therapeutic strategies to overcome chemoresistance and prevent recurrence in TNBC.

Sat Dec 12 00:00:00 EST 2020

Overexpression of Aiolos promotes epithelial-mesenchymal transition and cancer stem cell-like properties in lung cancer cells.

Scientific reports

Hung JJ,Kao YS,Huang CH,Hsu WH

17-1338 was used in Flow cytometry/Cell sorting to study the role of Aiolos/Ikaros family zinc finger 3 in epithelial-mesenchymal transition and cancer stem cell-like properties in lung cancer.

Thu Feb 28 00:00:00 EST 2019

Loss of Disabled-2 Expression in Pancreatic Cancer Progression.

Scientific reports

Hocevar BA

17-1338 was used in Flow cytometry/Cell sorting to identify Disabled-2 as a tumor suppressor by modulation of TGFβ-stimulated epithelial-to-mesenchymal transition and cancer stem cell phenotype.

Fri May 17 00:00:00 EDT 2019

ING5 activity in self-renewal of glioblastoma stem cells via calcium and follicle stimulating hormone pathways.

Oncogene

Wang F,Wang AY,Chesnelong C,Yang Y,Nabbi A,Thalappilly S,Alekseev V,Riabowol K

Published figure using CD133 (Prominin-1) monoclonal antibody (Product # 17-1338-42) in Flow Cytometry

Thu Jan 18 00:00:00 EST 2018

Inhibition of Fas associated phosphatase 1 (Fap1) facilitates apoptosis of colon cancer stem cells and enhances the effects of oxaliplatin.

Oncotarget

Huang W,Bei L,Eklund EA

Published figure using CD133 (Prominin-1) monoclonal antibody (Product # 17-1338-42) in Flow Cytometry

Fri May 25 00:00:00 EDT 2018

Immunophenotypic comparison of heterogenous non-sorted versus sorted mononuclear cells from human umbilical cord blood: a novel cell enrichment approach.

Cytotechnology

Indumathi S,Harikrishnan R,Rajkumar JS,Dhanasekaran M

17-1338 was used in Flow cytometry/Cell sorting to enrich the stem/progenitor cell population from human umbilical cord blood.

Thu Jan 01 00:00:00 EST 2015

Evaluation of cancer stem cell markers CD133, CD44, CD24: association with AKT isoforms and radiation resistance in colon cancer cells.

PloS one

Sahlberg SH,Spiegelberg D,Glimelius B,Stenerlöw B,Nestor M

Fri Jan 09 00:00:00 EST 2015

Comparative evaluation of differentiation potential of menstrual blood- versus bone marrow-derived stem cells into hepatocyte-like cells.

PloS one

Khanjani S,Khanmohammadi M,Zarnani AH,Akhondi MM,Ahani A,Ghaempanah Z,Naderi MM,Eghtesad S,Kazemnejad S

Sat Nov 08 00:00:00 EST 2014

Detection and validation of circulating endothelial cells, a blood-based diagnostic marker of acute myocardial infarction.

PloS one

Li C,Wu Q,Liu B,Yao Y,Chen Y,Zhang H,Wang C,Cao J,Ge S

17-1338-42 was used in Flow cytometry/Cell sorting to investigate the diagnostic and prognostic value of circulating endothelial cell monitoring in acute myocardial infarction patients.

Tue Sep 03 00:00:00 EDT 2013

Inhibition of p38 MAPK activity promotes ex vivo expansion of human cord blood hematopoietic stem cells.

Annals of hematology

Zou J,Zou P,Wang J,Li L,Wang Y,Zhou D,Liu L

17-1338 was used in Flow cytometry/Cell sorting to examine the effect of inhibition of p38 mitogen-activated protein kinase on expansion of human umbilical cord blood CD133(+) cells.

Fri Jun 01 00:00:00 EDT 2012

Bioinformatics

Protein Aliases: Antigen AC133; antigen CD133; CD133; hematopoietic stem cell antigen; hProminin; Lamp II; Lamp-2a; Lamp-2b; Lamp-2c; Lamp2; Mac3; Prominin-1; Prominin-like protein 1; Prominin1

View more View less

Gene Aliases: AC133; CD133; CORD12; MCDR2; MSTP061; PROM1; PROML1; RP41; STGD4

View more View less

UniProt ID: (Human) Q6SV49

View more View less

Entrez Gene ID: (Human) 8842

View more View less

Function(s)

protein binding actinin binding cadherin binding

Process(es)

retina layer formation photoreceptor cell maintenance retina morphogenesis in camera-type eye camera-type eye photoreceptor cell differentiation glomerular visceral epithelial cell differentiation glomerular parietal epithelial cell differentiation positive regulation of nephron tubule epithelial cell differentiation

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

Learn more

CD133 (Prominin-1) Monoclonal Antibody (TMP4), APC, eBioscience™

CD133 (Prominin-1) Antibody (17-1338-42) in Flow

Product Details

17-1338-42

Flow Cytometry (Flow)

Species Reactivity

Published species

Host/Isotype

Recommended Isotype Control

Class

Type

Clone

Conjugate

Excitation/Emission Max

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

How to use the Panel Builder

Bioinformatics

Performance Guarantee

We're here to help

Your items have has been added!