Invitrogen

CD154 (CD40 Ligand) Monoclonal Antibody (MR1), PE, eBioscience™

CD154 (CD40 Ligand) Antibody in Flow Cytometry (Flow)

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CD154 (CD40 Ligand) Antibody (12-1541-82) in Flow

T cells sorted from BALB/c splenocytes using the MagniSort™ Mouse T cell Enrichment Kit (Product # 8804-6820-74) were unstimulated (orange histogram) or stimulated with the Cell Stimulation Cocktail (Product # 00-4970-03) (purple histogram) and stained with 0.125 µg of Armenian Hamster IgG Isotype Control PE (Product # 12-4888-81) (left) or 0.125 µg of Anti-Mouse CD154 (CD40 Ligand) PE (right). Cells in the... View More

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Product Details

12-1541-82

Applications

Tested Dilution

Publications

Flow Cytometry (Flow)

0.25 µg/test

View 16 publications 16 publications

Product Specifications

Species Reactivity

Mouse

Published species

Mouse

Host/Isotype

Armenian hamster / IgG

Recommended Isotype Control

Armenian Hamster IgG Isotype Control (eBio299Arm), PE, eBioscience™

Class

Monoclonal

Type

Antibody

Clone

MR1

Conjugate

PE PE PE

Excitation/Emission Max

565/576 nm View spectra

Form

Liquid

Concentration

0.2 mg/mL

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.2

Contains

0.09% sodium azide

Storage conditions

4° C, store in dark, DO NOT FREEZE!

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_465887

Product Specific Information

Description: The MR1 monoclonal antibody reacts with mouse CD154, a 39 kDa transmembrane glycoprotein also known as CD40 ligand (CD40L). CD154 is expressed transiently by activated T cells. Through its binding to CD40 on antigen presenting cells (APC) including B cells, monocytes/macrophages, and dendritic cells, it serves a crucial function in T cell-APC cognate interaction. CD154-interaction with CD40 transduces signals for T-dependent B cell activation and induces B cells to enter the cell cycle.

For staining for flow cytometric analysis, it is important to stimulate enriched T cells or enriched CD4 cells (using depletion strategy) prior to staining with MR1.

Applications Reported: The MR1 antibody has been reported for use in flow cytometric analysis.

Applications Tested: This MR1 antibody has been tested by flow cytometric analysis of stimulated splenocytes. This can be used at less than or equal to 0.25 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

Excitation: 488-561 nm; Emission: 578 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser.

Filtration: 0.2 µm post-manufacturing filtered.

Target Information

CD40 Ligand (CD40-L), or CD154, is a membrane glycoprotein and differentiation antigen expressed on the surface of T cells. The CD40 Ligand stimulates B cell proliferation and secretion of all immunoglobulin isotypes in the presence of cytokines. CD40 Ligand has been shown to induce cytokine production and tumoricidal activity in peripheral blood monocytes. It also co-stimulates proliferation of activated T cells and this is accompanied by the production of IFN-gamma, TNF-alpha, and IL-2.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Product References

Strength of tonic T cell receptor signaling instructs T follicular helper cell-fate decisions.

Nature immunology

Bartleson JM,Viehmann Milam AA,Donermeyer DL,Horvath S,Xia Y,Egawa T,Allen PM

Published figure using CD154 (CD40 Ligand) monoclonal antibody (Product # 12-1541-82) in Flow Cytometry

Sun Nov 01 00:00:00 EDT 2020

Requirements for the differentiation of innate T-bet^high memory-phenotype CD4⁺ T lymphocytes under steady state.

Nature communications

Kawabe T,Yi J,Kawajiri A,Hilligan K,Fang D,Ishii N,Yamane H,Zhu J,Jankovic D,Kim KS,Trinchieri G,Sher A

12-1541 was used in Flow cytometry/Cell sorting to study whether MP CD4+ T cells are functionally heterogeneous and, if so, what signals specify the differentiation of MP cell subpopulations under homeostatic conditions.

Mon Jul 06 00:00:00 EDT 2020

CD103⁺ cDC1 and endogenous CD8⁺ T cells are necessary for improved CD40L-overexpressing CAR T cell antitumor function.

Nature communications

Kuhn NF,Lopez AV,Li X,Cai W,Daniyan AF,Brentjens RJ

12-1541 was used in Flow cytometry/Cell sorting to demonstrate the stimulatory effect of CD40L-overexpressing CAR T cells on innate and adaptive immune cells, and provide a rationale for using CD40L-overexpressing CAR T cells to improve immunotherapy responses.

Wed Dec 02 00:00:00 EST 2020

Manipulation of the tumor microenvironment by cytokine gene transfection enhances dendritic cell-based immunotherapy.

FASEB bioAdvances

Wijesekera DPH,Yuba E,De Silva NH,Watanabe SI,Tsukamoto M,Ichida C,Izawa T,Itoh K,Kanegi R,Hatoya S,Yamate J,Inaba T,Sugiura K

12-1541-82 was used in Flow Cytometry to manipulate the microenvironment so as to elicit activation of T helper type 1 (Th1) responses and the maturation of dendritic cells (DCs).

Wed Jan 01 00:00:00 EST 2020

Toxoplasma Co-infection Prevents Th2 Differentiation and Leads to a Helminth-Specific Th1 Response.

Frontiers in cellular and infection microbiology

Ahmed N,French T,Rausch S,Kühl A,Hemminger K,Dunay IR,Steinfelder S,Hartmann S

12-1541 was used in Flow cytometry/Cell sorting to conclude a previous T. gondii infection limits a helminth-specific Th2 immune response while promoting a shift toward a Th1-type immune response.

Mon Apr 02 00:00:00 EDT 2018

Interleukin-21 signaling in B cells, but not in T cells, is indispensable for the development of collagen-induced arthritis in mice.

Arthritis research & therapy

Sakuraba K,Oyamada A,Fujimura K,Spolski R,Iwamoto Y,Leonard WJ,Yoshikai Y,Yamada H

12-1541 was used in Flow cytometry/Cell sorting to study the role of IL-21 signalling in B and T cells in collagen-induced arthritis.

Wed Aug 17 00:00:00 EDT 2016

Elongated TCR alpha chain CDR3 favors an altered CD4 cytokine profile.

BMC biology

Reynolds C,Chong D,Raynsford E,Quigley K,Kelly D,Llewellyn-Hughes J,Altmann D,Boyton R

12-1541 was used in Flow cytometry/Cell sorting to study how the conformation of TCR alpha chain CDR3 favours different CD4 cytokine profiles.

Fri May 09 00:00:00 EDT 2014

Geranylgeranyl transferase regulates CXC chemokine formation in alveolar macrophages and neutrophil recruitment in septic lung injury.

American journal of physiology. Lung cellular and molecular physiology

Hasan Z,Rahman M,Palani K,Syk I,Jeppsson B,Thorlacius H

12-1541 was used in Flow cytometry/Cell sorting to conclude that inhibition of geranylgeranyl transferase might be a potent way to attenuate acute lung injury in abdominal sepsis.

Fri Feb 15 00:00:00 EST 2013

Detection of Foreign Antigen-specific CD4(+)Foxp3(+) Regulatory T Cells by MHC Class II Tetramer and Intracellular CD154 Staining.

Immune network

Choi JY,Eo SK

12-1541 was used in Flow cytometry/Cell sorting to evaluate the use of intracellular CD154 staining to detect and characterize foreign Ag-specific CD4+Foxp3+ regulatory T-cells in various models.

Sun Dec 01 00:00:00 EST 2013

IL-17RA is essential for optimal localization of follicular Th cells in the germinal center light zone to promote autoantibody-producing B cells.

Journal of immunology (Baltimore, Md. : 1950)

Ding Y,Li J,Wu Q,Yang P,Luo B,Xie S,Druey KM,Zajac AJ,Hsu HC,Mountz JD

12-1541 was used in Flow cytometry/Cell sorting to investigate the role of germinal centres in autoimmunity, showing that IL-17RA is essential for optimal localization of follicular Th cells in the germinal center light zone to promote autoantibody-producing B cells.

Thu Aug 15 00:00:00 EDT 2013

Helper cell-independent antitumor activity of potent CD8⁺ T cell epitope peptide vaccines is dependent upon CD40L.

Oncoimmunology

Llopiz D,Huarte E,Ruiz M,Bezunartea J,Belsúe V,Zabaleta A,Lasarte JJ,Prieto J,Borrás-Cuesta F,Sarobe P

Published figure using CD154 (CD40 Ligand) monoclonal antibody (Product # 12-1541-82) in Flow Cytometry

Sun Dec 01 00:00:00 EST 2013

Platelet shedding of CD40L is regulated by matrix metalloproteinase-9 in abdominal sepsis.

Journal of thrombosis and haemostasis : JTH

Rahman M,Zhang S,Chew M,Syk I,Jeppsson B,Thorlacius H

12-1541 was used in Flow cytometry/Cell sorting to investigate whether matrix metalloproteinase-9 cleaves surface-expressed CD40L and regulates pulmonary accumulation of neutrophils in sepsis.

Mon Jul 01 00:00:00 EDT 2013

ICOS expression by effector T cells influences the ability of regulatory T cells to inhibit anti-chromatin B cell responses in recipient mice.

Journal of autoimmunity

Hondowicz BD,Batheja AO,Metzgar MH,Caton AJ,Erikson J

12-1541 was used in Flow cytometry/Cell sorting to study the effect of elevated effector T-cell ICOS levels on immunity and immune cells.

Tue Jun 01 00:00:00 EDT 2010

Role of Valpha14+ NKT cells in the development of Hepatitis B virus-specific CTL: activation of Valpha14+ NKT cells promotes the breakage of CTL tolerance.

International immunology

Ito H,Ando K,Ishikawa T,Nakayama T,Taniguchi M,Saito K,Imawari M,Moriwaki H,Yokochi T,Kakumu S,Seishima M

12-1541 was used in Flow cytometry/Cell sorting to open up a new method for clearing the virus from patients with persistent HBV infection.

Tue Jul 01 00:00:00 EDT 2008

Naive CD4 T cells constitutively express CD40L and augment autoreactive B cell survival.

Proceedings of the National Academy of Sciences of the United States of America

Lesley R,Kelly LM,Xu Y,Cyster JG

Tue Jul 11 00:00:00 EDT 2006

Cytosolic entry controls CD8+-T-cell potency during bacterial infection.

Infection and immunity

Bahjat KS,Liu W,Lemmens EE,Schoenberger SP,Portnoy DA,Dubensky TW,Brockstedt DG

12-1541 was used in Flow cytometry/Cell sorting to investigate microbial invasion of the cytosol as a key factor in inducing CD8+- T cell-mediated protection against L.monocytogenes.

Wed Nov 01 00:00:00 EST 2006

Bioinformatics

Protein Aliases: CD154; CD40 ligand; CD40-L; H-CD-40-L; M-CD-40-L; RP23-153G22.3; T-cell antigen Gp39; TNF-related activation protein; TRAP; Tumor necrosis factor ligand superfamily member 5

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Gene Aliases: CD154; CD40-L; Cd40l; Cd40lg; gp39; HIGM1; IGM; IMD3; Ly-62; Ly62; T-BAM; Tnfsf5; TRAP

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UniProt ID: (Mouse) P27548

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Entrez Gene ID: (Mouse) 21947

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Function(s)

cytokine activity tumor necrosis factor receptor binding CD40 receptor binding

Process(es)

positive regulation of immune response to tumor cell inflammatory response immune response signal transduction platelet activation B cell differentiation positive regulation of B cell proliferation positive regulation of interferon-gamma production positive regulation of interleukin-10 production positive regulation of interleukin-12 production B cell proliferation negative regulation of apoptotic process isotype switching positive regulation of MHC class II biosynthetic process immunoglobulin secretion regulation of immunoglobulin secretion dendritic cell differentiation positive regulation of endothelial cell apoptotic process

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CD154 (CD40 Ligand) Monoclonal Antibody (MR1), PE, eBioscience™

CD154 (CD40 Ligand) Antibody (12-1541-82) in Flow

Product Details

12-1541-82

Flow Cytometry (Flow)

Species Reactivity

Published species

Host/Isotype

Recommended Isotype Control

Class

Type

Clone

Conjugate

Excitation/Emission Max

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

How to use the Panel Builder

Bioinformatics

Performance Guarantee

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