Invitrogen

CD154 (CD40 Ligand) Monoclonal Antibody (MR1), Functional Grade, eBioscience™

CD154 (CD40 Ligand) Antibody in Flow Cytometry (Flow)

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CD154 (CD40 Ligand) Antibody (16-1541-82) in Flow

Staining of 6-hour, PMA/Ionomycin-stimulated T cells from BALB/c spleen with 0.25 µg of Armenian Hamster IgG Isotype Control Functional Grade Purified (Product # 16-4888-81) (open histogram) or 0.25 µg of Anti-Mouse CD154 Functional Grade Purified (right) followed by Anti-Armenian Hamster IgG Biotin (Product # 13-4113-85) and Streptavidin PE (Product # 12-4317-87).Total viable cells were used for analysis.

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Product Details

16-1541-82

Applications

Tested Dilution

Publications

Flow Cytometry (Flow)

0.5 µg/test

View 11 publications 11 publications

Neutralization (Neu)

Assay-Dependent

View 1 publication 1 publication

Functional Assay (Functional)

Assay-Dependent

View 2 publications 2 publications

Inhibition Assays (IA)

View 1 publication 1 publication

Product Specifications

Species Reactivity

Mouse

Published species

Mouse

Host/Isotype

Armenian hamster / IgG

Recommended Isotype Control

Armenian Hamster IgG Isotype Control (eBio299Arm), Functional Grade, eBioscience™

Class

Monoclonal

Type

Antibody

Clone

MR1

Conjugate

Functional Grade Functional Grade Functional Grade

Form

Liquid

Concentration

1 mg/mL

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.2

Contains

no preservative

Storage conditions

4° C

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_469085

Product Specific Information

Description: The MR1 monoclonal antibody reacts with mouse CD154, a 39 kDa transmembrane glycoprotein also known as CD40 ligand (CD40L). CD154 is expressed transiently by activated T cells. Through its binding to CD40 on antigen presenting cells (APC) including B cells, monocytes/macrophages, and dendritic cells, it serves a crucial function in T cell-APC cognate interaction. CD154-interaction with CD40 transduces signals for T-dependent B cell activation and induces B cells to enter the cell cycle.

For staining for flow cytometric analysis, it is important to stimulate enriched T cells or enriched CD4 cells (using depletion strategy) prior to staining with MR1.

Applications Reported: The MR1 antibody has been reported for use in flow cytometric analysis. It has also been reported to inhibit binding of CD154 to CD40 and activation of B cells.

Applications Tested: This MR1 antibody has been tested by flow cytometric analysis of stimulated mouse splenocytes. This can be used at less than or equal to 0.5 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

Storage and handling: Use in a sterile environment.

Filtration: 0.2 µm post-manufacturing filtered.

Purity: Greater than 90%, as determined by SDS-PAGE.

Endotoxin Level: Less than 0.001 ng/µg antibody, as determined by LAL assay.

Aggregation: Less than 10%, as determined by HPLC.

Target Information

CD40 Ligand (CD40-L), or CD154, is a membrane glycoprotein and differentiation antigen expressed on the surface of T cells. The CD40 Ligand stimulates B cell proliferation and secretion of all immunoglobulin isotypes in the presence of cytokines. CD40 Ligand has been shown to induce cytokine production and tumoricidal activity in peripheral blood monocytes. It also co-stimulates proliferation of activated T cells and this is accompanied by the production of IFN-gamma, TNF-alpha, and IL-2.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Strength of tonic T cell receptor signaling instructs T follicular helper cell-fate decisions.

Nature immunology

Bartleson JM,Viehmann Milam AA,Donermeyer DL,Horvath S,Xia Y,Egawa T,Allen PM

Published figure using CD154 (CD40 Ligand) monoclonal antibody (Product # 16-1541-82) in Flow Cytometry

Sun Nov 01 00:00:00 EDT 2020

Reprogramming the adjuvant properties of aluminum oxyhydroxide with nanoparticle technology.

NPJ vaccines

Orr MT,Khandhar AP,Seydoux E,Liang H,Gage E,Mikasa T,Beebe EL,Rintala ND,Persson KH,Ahniyaz A,Carter D,Reed SG,Fox CB

Published figure using CD154 (CD40 Ligand) monoclonal antibody (Product # 16-1541-82) in Flow Cytometry

Thu Oct 01 00:00:00 EDT 2020

Cleavage of TL1A Differentially Regulates Its Effects on Innate and Adaptive Immune Cells.

Journal of immunology (Baltimore, Md. : 1950)

Ferdinand JR,Richard AC,Meylan F,Al-Shamkhani A,Siegel RM

16-1541 was used in Flow cytometry/Cell sorting to suggest differential roles for membrane and soluble TL1A on adaptive and innate immune cells and have implications for the consequences of blocking these two forms of TL1A.

Thu Feb 15 00:00:00 EST 2018

The transcription factor Runx3 guards cytotoxic CD8⁺ effector T cells against deviation towards follicular helper T cell lineage.

Nature immunology

Shan Q,Zeng Z,Xing S,Li F,Hartwig SM,Gullicksrud JA,Kurup SP,Van Braeckel-Budimir N,Su Y,Martin MD,Varga SM,Taniuchi I,Harty JT,Peng W,Badovinac VP,Xue HH

16-1541-82 was used in Flow Cytometry to show that Runx3-mediated Tcf7 repression coordinately enforced acquisition of cytotoxic functions and protected the cytotoxic lineage integrity by preventing TFH-lineage deviation.

Tue Aug 01 00:00:00 EDT 2017

Aging Converts Innate B1a Cells into Potent CD8+ T Cell Inducers.

Journal of immunology (Baltimore, Md. : 1950)

Lee-Chang C,Bodogai M,Moritoh K,Chen X,Wersto R,Sen R,Young HA,Croft M,Ferrucci L,Biragyn A

16-1541-82 was used in Inhibition Assays to indicate that aging affects the function of B1a cells.

Fri Apr 15 00:00:00 EDT 2016

Enhanced CD8 T cell responses through GITR-mediated costimulation resolve chronic viral infection.

PLoS pathogens

Pascutti MF,Geerman S,Slot E,van Gisbergen KP,Boon L,Arens R,van Lier RA,Wolkers MC,Nolte MA

16-1541 was used in Flow cytometry/Cell sorting to study resolution of chronic viral infection through GITR-mediated co-stimulation enhancing CD8 T cell responses.

Sun Mar 01 00:00:00 EST 2015

CD4⁺ and CD8⁺ T cell-dependent antiviral immunity requires STIM1 and STIM2.

The Journal of clinical investigation

Shaw PJ,Weidinger C,Vaeth M,Luethy K,Kaech SM,Feske S

16-1541 was used in Flow cytometry/Cell sorting to investigate the role of STIM1 and STIM2 in CD4+ and CD8+ T cell-dependent antiviral immunity.

Wed Oct 01 00:00:00 EDT 2014

Natural killer T cells are essential for the development of contact hypersensitivity in BALB/c mice.

The Journal of investigative dermatology

Shimizuhira C,Otsuka A,Honda T,Kitoh A,Egawa G,Nakajima S,Nakashima C,Watarai H,Miyachi Y,Kabashima K

16-1541 was used in Flow cytometry/Cell sorting to report the pivotal contribution of dendritic and natural killer T cell interactions to hypersensitivity.

Sat Nov 01 00:00:00 EDT 2014

Cancer immunotherapy employing an innovative strategy to enhance CD4+ T cell help in the tumor microenvironment.

PloS one

Song L,Yang MC,Knoff J,Wu TC,Hung CF

16-1541 was used in Flow cytometry/Cell sorting to investigate the effect of intratumoral injection of PADRE and the adjuvant CpG into HPV16 E7-expressing TC-1 tumours following cisplatin chemotherapy, showing potent antitumor effects and antigen-specific cell-mediated immune response.

Tue Dec 15 00:00:00 EST 2015

Helper cell-independent antitumor activity of potent CD8⁺ T cell epitope peptide vaccines is dependent upon CD40L.

Oncoimmunology

Llopiz D,Huarte E,Ruiz M,Bezunartea J,Belsúe V,Zabaleta A,Lasarte JJ,Prieto J,Borrás-Cuesta F,Sarobe P

Published figure using CD154 (CD40 Ligand) monoclonal antibody (Product # 16-1541-82) in Flow Cytometry

Sun Dec 01 00:00:00 EST 2013

A self-help program for memory CD8+ T cells: positive feedback via CD40-CD40L signaling as a critical determinant of secondary expansion.

PloS one

Shugart JA,Bambina S,Alice AF,Montler R,Bahjat KS

16-1541 was used in Flow cytometry/Cell sorting to demonstrate that vaccination regimens differ in their dependence on CD40L-expressing CD8+ T cells for secondary expansion.

Tue Jan 07 00:00:00 EST 2014

Constitutive CD40L expression on B cells prematurely terminates germinal center response and leads to augmented plasma cell production in T cell areas.

Journal of immunology (Baltimore, Md. : 1950)

Bolduc A,Long E,Stapler D,Cascalho M,Tsubata T,Koni PA,Shimoda M

16-1541 was used in Flow cytometry/Cell sorting to elucidate the role of CD40 signalling through cognate T-B interaction in the generation of germinal centre and memory B cells.

Thu Jul 01 00:00:00 EDT 2010

The Toll-IL-1R member Tir8/SIGIRR negatively regulates adaptive immunity against kidney grafts.

Journal of immunology (Baltimore, Md. : 1950)

Noris M,Cassis P,Azzollini N,Cavinato R,Cugini D,Casiraghi F,Aiello S,Solini S,Cassis L,Mister M,Todeschini M,Abbate M,Benigni A,Trionfini P,Tomasoni S,Mele C,Garlanda C,Polentarutti N,Mantovani A,Remuzzi G

16-1541 was used in Functional assays to test the hypothesis that Toll-IL-1R8, a negative regulator of TLR/IL-1R highly expressed in the kidney, modulates immune cell activation underlying kidney rejection.

Thu Oct 01 00:00:00 EDT 2009

Naive CD4 T cells constitutively express CD40L and augment autoreactive B cell survival.

Proceedings of the National Academy of Sciences of the United States of America

Lesley R,Kelly LM,Xu Y,Cyster JG

Published figure using CD154 (CD40 Ligand) monoclonal antibody (Product # 16-1541-82) in Flow Cytometry

Tue Jul 11 00:00:00 EDT 2006

Preventing restimulation of memory B cells in hemophilia A: a potential new strategy for the treatment of antibody-dependent immune disorders.

Blood

Hausl C,Ahmad RU,Schwarz HP,Muchitsch EM,Turecek PL,Dorner F,Reipert BM

16-1541 was used in Blocking experiments to study the requirements for the restimulation of FVIII-specific memory B cells and their differentiation into anti-FVIII antibody-producing cells.

Thu Jul 01 00:00:00 EDT 2004

Bioinformatics

Protein Aliases: CD154; CD40 ligand; CD40-L; H-CD-40-L; M-CD-40-L; RP23-153G22.3; T-cell antigen Gp39; TNF-related activation protein; TRAP; Tumor necrosis factor ligand superfamily member 5

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Gene Aliases: CD154; CD40-L; Cd40l; Cd40lg; gp39; HIGM1; IGM; IMD3; Ly-62; Ly62; T-BAM; Tnfsf5; TRAP

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UniProt ID: (Mouse) P27548

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Entrez Gene ID: (Mouse) 21947

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Function(s)

cytokine activity tumor necrosis factor receptor binding CD40 receptor binding

Process(es)

positive regulation of immune response to tumor cell inflammatory response immune response signal transduction platelet activation B cell differentiation positive regulation of B cell proliferation positive regulation of interferon-gamma production positive regulation of interleukin-10 production positive regulation of interleukin-12 production B cell proliferation negative regulation of apoptotic process isotype switching positive regulation of MHC class II biosynthetic process immunoglobulin secretion regulation of immunoglobulin secretion dendritic cell differentiation positive regulation of endothelial cell apoptotic process

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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CD154 (CD40 Ligand) Monoclonal Antibody (MR1), Functional Grade, eBioscience™

CD154 (CD40 Ligand) Antibody (16-1541-82) in Flow

Product Details

16-1541-82

Flow Cytometry (Flow)

Neutralization (Neu)

Functional Assay (Functional)

Inhibition Assays (IA)

Species Reactivity

Published species

Host/Isotype

Recommended Isotype Control

Class

Type

Clone

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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