Invitrogen

CD309 (FLK1) Monoclonal Antibody (Avas12a1), PE, eBioscience™

40 Published Figures

47 References

View all (71) FLK1 (VEGF Receptor 2) antibodies

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Datasheet

Protocols

Questions & Answers

Antibody Testing Data (1)
Published Figures (40)

CD309 (FLK1) Antibody in Flow Cytometry (Flow)

FIGURE: 1 / 41

CD309 (FLK1) Antibody (12-5821-82) in Flow

Staining of bEnd-3 cell line with 0.5 µg of Rat IgG2a K Isotype Control PE (Product # 12-4321-80) (open histogram) or 0.5 µg of Anti-Mouse CD309 (FLK1) PE (filled histogram). Total viable cells were used for analysis.

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Product Details

12-5821-82

Applications

Tested Dilution

Publications

Flow Cytometry (Flow)

0.5 µg/test

View 47 publications 47 publications

Product Specifications

Species Reactivity

Mouse

Published species

Human, Mouse

Host/Isotype

Rat / IgG2a, kappa

Recommended Isotype Control

Rat IgG2a kappa Isotype Control (eBR2a), PE, eBioscience™

Class

Monoclonal

Type

Antibody

Clone

Avas12a1

Conjugate

PE PE PE

Excitation/Emission Max

565/576 nm View spectra

Form

Liquid

Concentration

0.2 mg/mL

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.2

Contains

0.09% sodium azide

Storage conditions

4° C, store in dark, DO NOT FREEZE!

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_465967

Product Specific Information

Description: The Avas12a1 monoclonal antibody reacts with mouse Flk-1, also known as vascular endothelial growth factor receptor 2 (VEGFR2). Flk-1 is a receptor tyrosine kinase involved in vascular endothelial tissue development and is expressed on endothelial cells during embryonic stages and some endothelial tissues in the adult.

Applications Reported: The Avas12a1 antibody has been reported for use in flow cytometric analysis.

Applications Tested: The Avas12a1 antibody has been tested by flow cytometric analysis of bEnd.3 cells. This can be used at less than or equal to 0.5 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

Excitation: 488-561 nm; Emission: 578 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser.

Filtration: 0.2 µm post-manufacturing filtered.

Target Information

KDR (FLK1, VEGFR2) is a type III receptor tyrosine kinase. It binds vascular endothelial growth factor with high affinity in vitro, and is expressed early in development by endothelial cell precursors. FLK1 (VEGF Receptor 2) encodes one of the two receptors of the VEGF. This receptor, known as kinase insert domain receptor, is a type III receptor tyrosine kinase. The signalling and trafficking of this receptor are regulated by multiple factors, including Rab GTPase, P2Y purine nucleotide receptor, integrin alphaVbeta3, T-cell protein tyrosine phosphatase, etc. Mutations of FLK1 (VEGF Receptor 2) are implicated in infantile capillary hemangiomas.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Product References

CXCL5 suppression recovers neovascularization and accelerates wound healing in diabetes mellitus.

Cardiovascular diabetology

Chen C,Lin LY,Chen JW,Chang TT

12-5821-82 was used in Flow Cytometry to explore the impacts and mechanistic insights of CXCL5 in neovasculogenesis and wound healing in diabetes mellitus.

Fri Jul 07 00:00:00 EDT 2023

Lamin A/C-dependent chromatin architecture safeguards naïve pluripotency to prevent aberrant cardiovascular cell fate and function.

Nature communications

Wang Y,Elsherbiny A,Kessler L,Cordero J,Shi H,Serke H,Lityagina O,Trogisch FA,Mohammadi MM,El-Battrawy I,Backs J,Wieland T,Heineke J,Dobreva G

Published figure using CD309 (FLK1) monoclonal antibody (Product # 12-5821-82) in Flow Cytometry

Fri Nov 04 00:00:00 EDT 2022

Canonical Wnt signaling promotes pacemaker cell specification of cardiac mesodermal cells derived from mouse and human embryonic stem cells.

Stem cells (Dayton, Ohio)

Liang W,Han P,Kim EH,Mak J,Zhang R,Torrente AG,Goldhaber JI,Marbán E,Cho HC

12-5821-82 was used in Flow Cytometry to investigate the mechanisms for nodal pacemaker cell commitment.

Sun Mar 01 00:00:00 EST 2020

High expression of endothelial progenitor cell-induced angiogenic markers is associated with bile acid levels in HCC.

Oncology letters

Chen JL,Wang L,Li R,Jiao YF,Yu WF

12-5821 was used in Flow cytometry/Cell sorting to investigate the factors influencing the number of EPCs and circulating progenitor cells (CPCs), as well as the expression levels of vascular endothelial growth factor receptor 2 (VEGFR-2) and CD34, in patients with hepatocellular carcinoma.

Tue Sep 01 00:00:00 EDT 2020

Dual Targeting of Endothelial and Cancer Cells Potentiates In Vitro Nanobody-Targeted Photodynamic Therapy.

Cancers

Mashayekhi V,Xenaki KT,van Bergen En Henegouwen PMP,Oliveira S

12-5821 was used in Flow cytometry/Cell sorting to show that the anti-VEGFR2 conjugates are specific and potent PDT agents.

Wed Sep 23 00:00:00 EDT 2020

Simultaneous isolation of mesenchymal stem cells and endothelial progenitor cells derived from murine bone marrow.

Experimental and therapeutic medicine

Wang X,Zhao Z,Zhang H,Hou J,Feng W,Zhang M,Guo J,Xia J,Ge Q,Chen X,Wu X

12-5821-82 was used in Flow cytometry/Cell sorting to develop a method for the simultaneous isolation of homogenous populations of mesenchymal stem or stromal cells and endothelial progenitor cells from murine bone marrow.

Sat Dec 01 00:00:00 EST 2018

SCL/TAL1 cooperates with Polycomb RYBP-PRC1 to suppress alternative lineages in blood-fated cells.

Nature communications

Chagraoui H,Kristiansen MS,Ruiz JP,Serra-Barros A,Richter J,Hall-Ponselé E,Gray N,Waithe D,Clark K,Hublitz P,Repapi E,Otto G,Sopp P,Taylor S,Thongjuea S,Vyas P,Porcher C

12-5821 was used in Flow cytometry/Cell sorting to investigate how mesoderm specifies into blood-fated cells.

Tue Dec 18 00:00:00 EST 2018

Single-cell transcriptomics reveals a new dynamical function of transcription factors during embryonic hematopoiesis.

eLife

Bergiers I,Andrews T,Vargel Bölükbaşı Ö,Buness A,Janosz E,Lopez-Anguita N,Ganter K,Kosim K,Celen C,Itır Perçin G,Collier P,Baying B,Benes V,Hemberg M,Lancrin C

Published figure using CD309 (FLK1) monoclonal antibody (Product # 12-5821-82) in Flow Cytometry

Tue Mar 20 00:00:00 EDT 2018

Antifibrotic role of vascular endothelial growth factor in pulmonary fibrosis.

JCI insight

Murray LA,Habiel DM,Hohmann M,Camelo A,Shang H,Zhou Y,Coelho AL,Peng X,Gulati M,Crestani B,Sleeman MA,Mustelin T,Moore MW,Ryu C,Osafo-Addo AD,Elias JA,Lee CG,Hu B,Herazo-Maya JD,Knight DA,Hogaboam CM,Herzog EL

12-5821 was used in Flow cytometry/Cell sorting to investigate the association of VEGF with the idiopathic pulmonary fibrosis disease state.

Thu Aug 17 00:00:00 EDT 2017

Cancer cell-secreted IGF2 instigates fibroblasts and bone marrow-derived vascular progenitor cells to promote cancer progression.

Nature communications

Xu WW,Li B,Guan XY,Chung SK,Wang Y,Yip YL,Law SY,Chan KT,Lee NP,Chan KW,Xu LY,Li EM,Tsao SW,He QY,Cheung AL

12-5821 was used in Flow cytometry/Cell sorting to investigate how the Id1/IGF2/VEGF/VEGFR1 cascade plays a critical role in tumour-driven pathophysiological processes underlying cancer progression.

Fri Feb 10 00:00:00 EST 2017

Cancer cell-secreted IGF2 instigates fibroblasts and bone marrow-derived vascular progenitor cells to promote cancer progression.

Nature communications

Xu WW,Li B,Guan XY,Chung SK,Wang Y,Yip YL,Law SY,Chan KT,Lee NP,Chan KW,Xu LY,Li EM,Tsao SW,He QY,Cheung AL

12-5821 was used in Flow cytometry/Cell sorting to investigate how the Id1/IGF2/VEGF/VEGFR1 cascade plays a critical role in tumour-driven pathophysiological processes underlying cancer progression.

Fri Feb 10 00:00:00 EST 2017

Arginase inhibition suppresses lung metastasis in the 4T1 breast cancer model independently of the immunomodulatory and anti-metastatic effects of VEGFR-2 blockade.

Oncoimmunology

Secondini C,Coquoz O,Spagnuolo L,Spinetti T,Peyvandi S,Ciarloni L,Botta F,Bourquin C,Rüegg C

12-5821 was used in Flow cytometry/Cell sorting to investigate the effect of VEGF/VEGFR-2 axis inhibition on lung metastasis, myeloid-derived suppressor cells and regulatory T cells.

Tue Jan 12 00:00:00 EST 2021

EphrinB2 regulates the emergence of a hemogenic endothelium from the aorta.

Scientific reports

Chen II,Caprioli A,Ohnuki H,Kwak H,Porcher C,Tosato G

12-5821 was used in Flow cytometry/Cell sorting to show that EphrinB2 regulates the emergence of a hemogenic endothelium from the aorta.

Thu Jun 02 00:00:00 EDT 2016

The Isl1/Ldb1 Complex Orchestrates Genome-wide Chromatin Organization to Instruct Differentiation of Multipotent Cardiac Progenitors.

Cell stem cell

Caputo L,Witzel HR,Kolovos P,Cheedipudi S,Looso M,Mylona A,van IJcken WF,Laugwitz KL,Evans SM,Braun T,Soler E,Grosveld F,Dobreva G

Published figure using CD309 (FLK1) monoclonal antibody (Product # 12-5821-82) in Flow Cytometry

Thu Sep 03 00:00:00 EDT 2015

The C-terminal CGHC motif of protein disulfide isomerase supports thrombosis.

The Journal of clinical investigation

Zhou J,Wu Y,Wang L,Rauova L,Hayes VM,Poncz M,Essex DW

12-5821 was used in Flow cytometry/Cell sorting to investigate the contribution of protein disulfide isomerase CGHC sites during physiological reaction such as thrombosis.

Tue Nov 03 00:00:00 EST 2015

Mouse bone marrow-derived mesenchymal stem cells inhibit leukemia/lymphoma cell proliferation in vitro and in a mouse model of allogeneic bone marrow transplant.

International journal of molecular medicine

Song N,Gao L,Qiu H,Huang C,Cheng H,Zhou H,Lv S,Chen L,Wang J

12-5821 was used in Flow cytometry/Cell sorting to determine that MSCs may be safe and effective when used in allogeneic BMT for the treatment of haemotological malignancies.

Wed Jul 01 00:00:00 EDT 2015

Endoglin potentiates nitric oxide synthesis to enhance definitive hematopoiesis.

Biology open

Nasrallah R,Knezevic K,Thai T,Thomas SR,Göttgens B,Lacaud G,Kouskoff V,Pimanda JE

12582182 was used in flow cytometry to investigate the role of endoglin in the development of hematopoietic cells.

Fri May 15 00:00:00 EDT 2015

Histone Demethylases KDM4A and KDM4C Regulate Differentiation of Embryonic Stem Cells to Endothelial Cells.

Stem cell reports

Wu L,Wary KK,Revskoy S,Gao X,Tsang K,Komarova YA,Rehman J,Malik AB

Published figure using CD309 (FLK1) monoclonal antibody (Product # 12-5821-82) in Flow Cytometry

Tue Jul 14 00:00:00 EDT 2015

Rivaroxaban, a factor Xa inhibitor, improves neovascularization in the ischemic hindlimb of streptozotocin-induced diabetic mice.

Cardiovascular diabetology

Wu TC,Chan JS,Lee CY,Leu HB,Huang PH,Chen JS,Lin SJ,Chen JW

12-5821 was used in Flow cytometry/Cell sorting to demonstrate that rivaroxaban promotes vessel formation in diabetic mice and improves endothelial progenitor cell function under hyperglycemic conditions.

Tue Jun 16 00:00:00 EDT 2015

Mesenchymal-endothelial transition contributes to cardiac neovascularization.

Nature

Ubil E,Duan J,Pillai IC,Rosa-Garrido M,Wu Y,Bargiacchi F,Lu Y,Stanbouly S,Huang J,Rojas M,Vondriska TM,Stefani E,Deb A

Published figure using CD309 (FLK1) monoclonal antibody (Product # 12-5821-82) in Flow Cytometry

Thu Oct 30 00:00:00 EDT 2014

The miR-126-VEGFR2 axis controls the innate response to pathogen-associated nucleic acids.

Nature immunology

Agudo J,Ruzo A,Tung N,Salmon H,Leboeuf M,Hashimoto D,Becker C,Garrett-Sinha LA,Baccarini A,Merad M,Brown BD

12-5821 was used in Flow cytometry/Cell sorting to identify the miR-126-VEGFR2 axis as an important regulator of the innate response that operates through multiscale control of pDCs.

Wed Jan 01 00:00:00 EST 2014

BMP signaling balances murine myeloid potential through SMAD-independent p38MAPK and NOTCH pathways.

Blood

Cook BD,Evans T

12-5821 was used in Flow cytometry/Cell sorting to investigate SMAD-independent control of haematopoietic potential in mice, identifying a previously unrecognised bone morphogenetic protein signalling axis.

Thu Jul 17 00:00:00 EDT 2014

SOX9 accelerates ESC differentiation to three germ layer lineages by repressing SOX2 expression through P21 (WAF1/CIP1).

Development (Cambridge, England)

Yamamizu K,Schlessinger D,Ko MS

Published figure using CD309 (FLK1) monoclonal antibody (Product # 12-5821-82) in Flow Cytometry

Sat Nov 01 00:00:00 EDT 2014

Expression of podocalyxin separates the hematopoietic and vascular potentials of mouse embryonic stem cell-derived mesoderm.

Stem cells (Dayton, Ohio)

Zhang H,Nieves JL,Fraser ST,Isern J,Douvaras P,Papatsenko D,D'Souza SL,Lemischka IR,Dyer MA,Baron MH

Published figure using CD309 (FLK1) monoclonal antibody (Product # 12-5821-82) in Flow Cytometry

Wed Jan 01 00:00:00 EST 2014

Sox7 is regulated by ETV2 during cardiovascular development.

Stem cells and development

Behrens AN,Zierold C,Shi X,Ren Y,Koyano-Nakagawa N,Garry DJ,Martin CM

12-5821 was used in Flow cytometry/Cell sorting to investigate the mechanistic interaction between Sox7 and Etv2 during cardiovascular development.

Mon Sep 01 00:00:00 EDT 2014

Cooperative interaction of Etv2 and Gata2 regulates the development of endothelial and hematopoietic lineages.

Developmental biology

Shi X,Richard J,Zirbes KM,Gong W,Lin G,Kyba M,Thomson JA,Koyano-Nakagawa N,Garry DJ

Published figure using CD309 (FLK1) monoclonal antibody (Product # 12-5821-82) in Flow Cytometry

Thu May 15 00:00:00 EDT 2014

Gene targeting study reveals unexpected expression of brain-expressed X-linked 2 in endocrine and tissue stem/progenitor cells in mice.

The Journal of biological chemistry

Ito K,Yamazaki S,Yamamoto R,Tajima Y,Yanagida A,Kobayashi T,Kato-Itoh M,Kakuta S,Iwakura Y,Nakauchi H,Kamiya A

12-5821 was used in Flow cytometry/Cell sorting to investigate the precise role and expression pattern of brain-expressed X-linked genes in endocrine and tissue stem/progenitor cells in mice.

Fri Oct 24 00:00:00 EDT 2014

Nkx2-5 mediates differential cardiac differentiation through interaction with Hoxa10.

Stem cells and development

Behrens AN,Iacovino M,Lohr JL,Ren Y,Zierold C,Harvey RP,Kyba M,Garry DJ,Martin CM

Thu Aug 01 00:00:00 EDT 2013

Etv2 rescues Flk1 mutant embryoid bodies.

Genesis (New York, N.Y. : 2000)

Rasmussen TL,Martin CM,Walter CA,Shi X,Perlingeiro R,Koyano-Nakagawa N,Garry DJ

Published figure using CD309 (FLK1) monoclonal antibody (Product # 12-5821-82) in Flow Cytometry

Mon Jul 01 00:00:00 EDT 2013

Ectopic Runx1 expression rescues Tal-1-deficiency in the generation of primitive and definitive hematopoiesis.

PloS one

Tornack J,Seiler K,Grützkau A,Grün JR,Onodera M,Melchers F,Tsuneto M

12-5821 was used in Flow cytometry/Cell sorting to suggest that Tal-1 expression is needed to express Runx1 in the mesoderm.

Thu Aug 21 00:00:00 EDT 2014

Molecular imaging of bone marrow mononuclear cell survival and homing in murine peripheral artery disease.

JACC. Cardiovascular imaging

van der Bogt KE,Hellingman AA,Lijkwan MA,Bos EJ,de Vries MR,van Rappard JR,Fischbein MP,Quax PH,Robbins RC,Hamming JF,Wu JC

12-5821 was used in Flow cytometry/Cell sorting to assess the kinetics of transplanted MNCs in PAD using in vivo molecular imaging.

Sun Jan 01 00:00:00 EST 2012

Sympathetic nervous system regulates bone marrow-derived cell egress through endothelial nitric oxide synthase activation: role in postischemic tissue remodeling.

Arteriosclerosis, thrombosis, and vascular biology

Récalde A,Richart A,Guérin C,Cochain C,Zouggari Y,Yin KH,Vilar J,Drouet I,Lévy B,Varoquaux O,Silvestre JS

12-5821 was used in Flow cytometry/Cell sorting to describe a major role for the sympathetic nervous system in BM-derived cell egress through stromal eNOS activation.

Thu Mar 01 00:00:00 EST 2012

Etv2 is expressed in the yolk sac hematopoietic and endothelial progenitors and regulates Lmo2 gene expression.

Stem cells (Dayton, Ohio)

Koyano-Nakagawa N,Kweon J,Iacovino M,Shi X,Rasmussen TL,Borges L,Zirbes KM,Li T,Perlingeiro RC,Kyba M,Garry DJ

Published figure using CD309 (FLK1) monoclonal antibody (Product # 12-5821-82) in Flow Cytometry

Wed Aug 01 00:00:00 EDT 2012

Snail promotes the cell-autonomous generation of Flk1(+) endothelial cells through the repression of the microRNA-200 family.

Stem cells and development

Gill JG,Langer EM,Lindsley RC,Cai M,Murphy TL,Murphy KM

12-5821 was used in Flow cytometry/Cell sorting to assess the role of snail to promote the cell-autonomous induction of Flk1(+) endothelial cells in mouse embryonic stem cells.

Fri Jan 20 00:00:00 EST 2012

VEGF/Flk1 signaling cascade transactivates Etv2 gene expression.

PloS one

Rasmussen TL,Shi X,Wallis A,Kweon J,Zirbes KM,Koyano-Nakagawa N,Garry DJ

Published figure using CD309 (FLK1) monoclonal antibody (Product # 12-5821-82) in Flow Cytometry

Mon May 13 00:00:00 EDT 2013

The notch ligand delta-like 4 regulates multiple stages of early hemato-vascular development.

PloS one

Laranjeiro R,Alcobia I,Neves H,Gomes AC,Saavedra P,Carvalho CC,Duarte A,Cidadão A,Parreira L

12-5821 was used in Flow cytometry/Cell sorting to indicate a role for the notch ligand delta-like 4 in the quantitative regulation of early hemato-vascular precursors.

Thu Aug 30 00:00:00 EDT 2012

Recapitulation of the embryonic cardiovascular progenitor cell niche.

Biomaterials

Schenke-Layland K,Nsair A,Van Handel B,Angelis E,Gluck JM,Votteler M,Goldhaber JI,Mikkola HK,Kahn M,Maclellan WR

Published figure using CD309 (FLK1) monoclonal antibody (Product # 12-5821-82) in Flow Cytometry

Fri Apr 01 00:00:00 EDT 2011

Snail and the microRNA-200 family act in opposition to regulate epithelial-to-mesenchymal transition and germ layer fate restriction in differentiating ESCs.

Stem cells (Dayton, Ohio)

Gill JG,Langer EM,Lindsley RC,Cai M,Murphy TL,Kyba M,Murphy KM

12-5821 was used in Flow cytometry/Cell sorting to investigate the role of Snail and microRNA-200 in regulating epithelial-to-mesenchymal transition and embryonic stem cell differentiation.

Sun May 01 00:00:00 EDT 2011

Assessment of the myogenic stem cell compartment following transplantation of Pax3/Pax7-induced embryonic stem cell-derived progenitors.

Stem cells (Dayton, Ohio)

Darabi R,Santos FN,Filareto A,Pan W,Koene R,Rudnicki MA,Kyba M,Perlingeiro RC

12-5821 was used in Flow cytometry/Cell sorting to investigate Pax3/Pax7-induced embryonic stem cell-derived progenitors as a therapeutic application of ES-derived myogenic progenitors in muscle disorders.

Sun May 01 00:00:00 EDT 2011

A triple-targeted ultrasound contrast agent provides improved localization to tumor vasculature.

Journal of ultrasound in medicine : official journal of the American Institute of Ultrasound in Medicine

Warram JM,Sorace AG,Saini R,Umphrey HR,Zinn KR,Hoyt K

Published figure using CD309 (FLK1) monoclonal antibody (Product # 12-5821-82) in Flow Cytometry

Fri Jul 01 00:00:00 EDT 2011

Hepatoblast-like progenitor cells derived from embryonic stem cells can repopulate livers of mice.

Gastroenterology

Li F,Liu P,Liu C,Xiang D,Deng L,Li W,Wangensteen K,Song J,Ma Y,Hui L,Wei L,Li L,Ding X,Hu Y,He Z,Wang X

12-5821 was used in Flow cytometry/Cell sorting to identify and purify ES cell-derived hepatoblast-like progenitor cells and to explore their capacity for liver repopulation in mice after in vitro expansion.

Wed Dec 01 00:00:00 EST 2010

Major histocompatibility complex-I expression on embryonic stem cell-derived vascular progenitor cells is critical for syngeneic transplant survival.

Stem cells (Dayton, Ohio)

Ma M,Ding S,Lundqvist A,San H,Fang F,Konoplyannikov M,Berry C,Beltran LE,Chen G,Kovacic JC,Boehm M

Published figure using CD309 (FLK1) monoclonal antibody (Product # 12-5821-82) in Flow Cytometry

Wed Sep 01 00:00:00 EDT 2010

Mesenchymal origin of hepatic stellate cells, submesothelial cells, and perivascular mesenchymal cells during mouse liver development.

Hepatology (Baltimore, Md.)

Asahina K,Tsai SY,Li P,Ishii M,Maxson RE,Sucov HM,Tsukamoto H

Published figure using CD309 (FLK1) monoclonal antibody (Product # 12-5821-82) in Flow Cytometry

Sun Mar 01 00:00:00 EST 2009

Nox2-containing NADPH oxidase deficiency confers protection from hindlimb ischemia in conditions of increased oxidative stress.

Arteriosclerosis, thrombosis, and vascular biology

Haddad P,Dussault S,Groleau J,Turgeon J,Michaud SE,Ménard C,Perez G,Maingrette F,Rivard A

12-5821 was used in Flow cytometry/Cell sorting to investigate the role for Nox2-containing NADPH oxidase in the modulation of neovascularisation in conditions of increased oxidative stress.

Thu Oct 01 00:00:00 EDT 2009

Voluntary running suppresses proinflammatory cytokines and bone marrow endothelial progenitor cell levels in apolipoprotein-E-deficient mice.

Antioxidants & redox signaling

Ajijola OA,Dong C,Herderick EE,Ma Q,Goldschmidt-Clermont PJ,Yan Z

12-5821 was used in Flow cytometry/Cell sorting to determine whether exercise-induced enhancement of endothelial progenitor cells is associated with reduced atherosclerosis and inflammation.

Thu Jan 01 00:00:00 EST 2009

A critical role of IFNgamma in priming MSC-mediated suppression of T cell proliferation through up-regulation of B7-H1.

Cell research

Sheng H,Wang Y,Jin Y,Zhang Q,Zhang Y,Wang L,Shen B,Yin S,Liu W,Cui L,Li N

12-5821 was used in Flow cytometry/Cell sorting to investigate the role that IFNγ plays in mesenchymal stem cell mediated suppression of T cell proliferation.

Fri Aug 01 00:00:00 EDT 2008

Important role of erythropoietin receptor to promote VEGF expression and angiogenesis in peripheral ischemia in mice.

Circulation research

Nakano M,Satoh K,Fukumoto Y,Ito Y,Kagaya Y,Ishii N,Sugamura K,Shimokawa H

12-5821 was used in Flow cytometry/Cell sorting to indicate that vascular EpoR system also plays an important role in angiogenesis in response to hindlimb ischemia.

Fri Mar 16 00:00:00 EDT 2007

VEGF-A and FGF-2 synergistically promote neoangiogenesis through enhancement of endogenous PDGF-B-PDGFRbeta signaling.

Journal of cell science

Kano MR,Morishita Y,Iwata C,Iwasaka S,Watabe T,Ouchi Y,Miyazono K,Miyazawa K

12-5821 was used in Flow cytometry/Cell sorting to demonstrate that enhancement of endogenous PDGF-B-PDGFR signaling is critical for the synergistic effect of VEGF-A and FGF-2 on neoangiogenesis in adults.

Mon Aug 15 00:00:00 EDT 2005

Bioinformatics

Protein Aliases: CD309; CD309 antigen; Fetal liver kinase 1; FLK-1; Kinase NYK; Protein-tyrosine kinase receptor flk-1; sCD309; soluble CD309; soluble vascular endothelial growth factor receptor 2; soluble VEGFR 2; Vascular endothelial growth factor receptor 2; vascular endothelial growth factor receptor- 2; vascular endothelial growth factor receptor-2; vascular endothelial growth factor receptor-3; VEGF R2; VEGF receptor-2; VEGFR-2

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Gene Aliases: 6130401C07; Flk-1; Flk1; Kdr; Krd-1; Ly73; sVEGFR-2; VEGFR-2; VEGFR2

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UniProt ID: (Mouse) P35918

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Entrez Gene ID: (Mouse) 16542

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Function(s)

nucleotide binding protein kinase activity protein tyrosine kinase activity transmembrane receptor protein tyrosine kinase activity vascular endothelial growth factor-activated receptor activity integrin binding protein binding ATP binding kinase activity transferase activity transferase activity, transferring phosphorus-containing groups growth factor binding vascular endothelial growth factor binding transmembrane signal receptor

Process(es)

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CD309 (FLK1) Monoclonal Antibody (Avas12a1), PE, eBioscience™

CD309 (FLK1) Antibody (12-5821-82) in Flow

Product Details

12-5821-82

Flow Cytometry (Flow)

Species Reactivity

Published species

Host/Isotype

Recommended Isotype Control

Class

Type

Clone

Conjugate

Excitation/Emission Max

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

How to use the Panel Builder

Bioinformatics

Performance Guarantee

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