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Invitrogen
CD309 (FLK1) Monoclonal Antibody (Avas12a1), Super Bright™ 600, eBioscience™
FIGURE: 1 / 7
CD309 (FLK1) Antibody (63-5821-82) in Flow
bEnd.3 cells were stained with 0.5 µg of Rat IgG2a kappa Isotype Control, Super Bright 600 (Product # 63-4321-82) (blue histogram) or 0.5 µg of CD309 (Flk-1) Monoclonal Antibody, Super Bright 600 (purple histogram). Viable cells were used for analysis.
Product Details
63-5821-82
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Mouse
Published species
Not Applicable
Host/Isotype
Rat
/ IgG2a, kappa
Recommended Isotype Control
Class
Monoclonal
Type
Antibody
Clone
Avas12a1
Conjugate
Excitation/Emission Max
414/601 nm
View spectra
Form
Liquid
Concentration
0.2 mg/mL
Purification
Affinity chromatography
Storage buffer
PBS, pH 7.2, with BSA
Contains
0.09% sodium azide
Storage conditions
4° C, store in dark, DO NOT FREEZE!
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2688141
Product Specific Information
Description: The Avas12a1 monoclonal antibody reacts with mouse Flk-1, also known as vascular endothelial growth factor receptor 2 (VEGFR2) and CD309. Flk-1 is a receptor tyrosine kinase involved in vascular endothelial tissue development and is expressed on endothelial cells during embryonic stages and some endothelial tissues in the adult.
Applications Reported: This Avas12a1 antibody has been reported for use in flow cytometric analysis.
Applications Tested: This Avas12a1 antibody has been tested by flow cytometric analysis of bEnd.3 cells. This may be used at less than or equal to 1.0 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.
Super Bright 600 is a tandem dye that can be excited with the violet laser line (405 nm) and emits at 600 nm. We recommend using a 610/20 bandpass filter. Please make sure that your instrument is capable of detecting this fluorochrome.
When using two or more Super Bright dye-conjugated antibodies in a staining panel, it is recommended to use Super Bright Complete Staining Buffer (Product # SB-4401) to minimize any non-specific polymer interactions. Please refer to the datasheet for Super Bright Staining Buffer for more information.
Light sensitivity: This tandem dye is sensitive to photo-induced oxidation. Please protect this vial and stained samples from light.
Fixation: Samples can be stored in IC Fixation Buffer (Product # 00-8222) (100 µL of cell sample + 100 µL of IC Fixation Buffer) or 1-step Fix/Lyse Solution (Product # 00-5333) for up to 3 days in the dark at 4°C with minimal impact on brightness and FRET efficiency/compensation. Some generalizations regarding fluorophore performance after fixation can be made, but clone specific performance should be determined empirically.
Excitation: 405 nm; Emission: 600 nm; Laser: Violet Laser
Super Bright Polymer Dyes are sold under license from Becton, Dickinson and Company.
Target Information
KDR (FLK1, VEGFR2) is a type III receptor tyrosine kinase. It binds vascular endothelial growth factor with high affinity in vitro, and is expressed early in development by endothelial cell precursors. FLK1 (VEGF Receptor 2) encodes one of the two receptors of the VEGF. This receptor, known as kinase insert domain receptor, is a type III receptor tyrosine kinase. The signalling and trafficking of this receptor are regulated by multiple factors, including Rab GTPase, P2Y purine nucleotide receptor, integrin alphaVbeta3, T-cell protein tyrosine phosphatase, etc. Mutations of FLK1 (VEGF Receptor 2) are implicated in infantile capillary hemangiomas.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
How to use the Panel Builder
Watch the video to learn how to use the Invitrogen Flow Cytometry Panel Builder to build your next flow cytometry panel in 5 easy steps.
Bioinformatics
Protein Aliases: CD309; CD309 antigen; Fetal liver kinase 1; FLK-1; Kinase NYK; Protein-tyrosine kinase receptor flk-1; sCD309; soluble CD309; soluble vascular endothelial growth factor receptor 2; soluble VEGFR 2; Vascular endothelial growth factor receptor 2; vascular endothelial growth factor receptor- 2; vascular endothelial growth factor receptor-2; vascular endothelial growth factor receptor-3; VEGF R2; VEGF receptor-2; VEGFR-2
Gene Aliases: 6130401C07; Flk-1; Flk1; Kdr; Krd-1; Ly73; sVEGFR-2; VEGFR-2; VEGFR2
UniProt ID: (Mouse) P35918
Entrez Gene ID: (Mouse) 16542
nucleotide binding
protein kinase activity
protein tyrosine kinase activity
transmembrane receptor protein tyrosine kinase activity
vascular endothelial growth factor-activated receptor activity
integrin binding
protein binding
ATP binding
kinase activity
transferase activity
transferase activity, transferring phosphorus-containing groups
growth factor binding
vascular endothelial growth factor binding
transmembrane signal receptor
angiogenesis
ovarian follicle development
vasculogenesis
response to hypoxia
positive regulation of protein phosphorylation
regulation of endothelial cell proliferation
positive regulation of endothelial cell proliferation
lymph vessel development
cell migration involved in sprouting angiogenesis
positive regulation of mesenchymal cell proliferation
endocardium development
endothelium development
endochondral bone growth
protein phosphorylation
transmembrane receptor protein tyrosine kinase signaling pathway
positive regulation of cytosolic calcium ion concentration
multicellular organism development
positive regulation of cell proliferation
regulation of cell shape
male gonad development
positive regulation of endothelial cell migration
positive regulation of phosphatidylinositol 3-kinase signaling
phosphorylation
cell migration
peptidyl-tyrosine phosphorylation
hemopoiesis
cell differentiation
lung development
positive regulation of cell migration
positive regulation of BMP signaling pathway
positive regulation of vascular endothelial growth factor receptor signaling pathway
positive regulation of TOR signaling
embryonic hemopoiesis
calcium-mediated signaling using intracellular calcium source
cellular response to vascular endothelial growth factor stimulus
peptidyl-tyrosine autophosphorylation
vascular endothelial growth factor signaling pathway
response to drug
negative regulation of apoptotic process
surfactant homeostasis
positive regulation of MAPK cascade
negative regulation of neuron apoptotic process
cell fate commitment
endothelial cell differentiation
regulation of endothelial cell differentiation
positive regulation of angiogenesis
positive regulation of vasodilation
protein autophosphorylation
vascular endothelial growth factor receptor signaling pathway
positive regulation of long-term neuronal synaptic plasticity
lung alveolus development
cell maturation
branching morphogenesis of an epithelial tube
neuron projection morphogenesis
positive regulation of epithelial cell proliferation
positive regulation of calcium-mediated signaling
positive regulation of positive chemotaxis
positive regulation of nitric-oxide synthase biosynthetic process
positive regulation of focal adhesion assembly
calcium ion homeostasis
blood vessel endothelial cell differentiation
positive regulation of ERK1 and ERK2 cascade
regulation of hematopoietic progenitor cell differentiation
negative regulation of endothelial cell apoptotic process
positive regulation of vasculogenesis
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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