Invitrogen

CD357 (AITR/GITR) Monoclonal Antibody (DTA-1), PE, eBioscience™

CD357 (AITR/GITR) Antibody in Flow Cytometry (Flow)

FIGURE: 1 / 1

CD357 (AITR/GITR) Antibody (12-5874-82) in Flow

Staining of C57BL/6 splenocytes with Anti-Mouse CD4 APC (Product # 17-0042-82), Anti-Mouse CD25 eFluor® 450 (Product # 48-0253-82) and 0.002 µg of Rat IgG2b K Isotype Control PE (Product # 12-4031-82) (left) or 0.002 µg of Anti-Mouse CD357 (GITR) PE (right). Cells in the CD4+ lymphocyte gate were used for analysis.

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Product Details

12-5874-82

Applications

Tested Dilution

Publications

Flow Cytometry (Flow)

0.004 µg/test

View 16 publications 16 publications

Product Specifications

Species Reactivity

Mouse

Published species

Mouse

Host/Isotype

Rat / IgG2b, kappa

Recommended Isotype Control

Rat IgG2b kappa Isotype Control (eB149/10H5), PE, eBioscience™

Class

Monoclonal

Type

Antibody

Clone

DTA-1

Conjugate

PE PE PE

Excitation/Emission Max

565/576 nm View spectra

Form

Liquid

Concentration

0.2 mg/mL

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.2

Contains

0.09% sodium azide

Storage conditions

4° C, store in dark, DO NOT FREEZE!

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_465986

Product Specific Information

Description: The DTA-1 monoclonal antibody reacts with mouse GITR, Glucocorticoid-Induced TNFR family gene, also known as TNFRSF18. In naive mice, GITR is expressed predominantly by CD4+CD25+ T regulatory cells (Treg) and by CD25+ CD4+ CD8- thymocytes. Stimulation of GITR with DTA-1 antibody abrogates Treg cell-mediated suppression. Although it does not deplete CD357 expressing cells in vivo, it has been shown to induce tumorogenesis. The removal of GITR-expressing Treg cells or the administration of DTA-1 resulted in organ specific autoimmune disease.

Applications Reported: The DTA-1 antibody has been reported for use in flow cytometric analysis.

Applications Tested: This DTA-1 antibody has been tested by flow cytometric analysis of mouse splenocytes. This can be used at less than or equal to 0.004 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

Excitation: 488-561 nm; Emission: 578 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser.

Filtration: 0.2 µm post-manufacturing filtered.

Target Information

Tumor necrosis factor receptor superfamily member 18 (TNFRSF18), also called GITR or AITR is a protein that in humans is encoded by the TNFRSF18 gene. It is mapped to 1p36.33. This gene encodes a member of the TNF-receptor superfamily. The encoded receptor has been shown to have increased expression upon T-cell activation, and it is thought to play a key role in dominant immunological self-tolerance maintained by CD25 (+)CD4 (+) regulatory T cells. Knockout studies in mice also suggest the role of this receptor is in the regulation of CD3-driven T-cell activation and programmed cell death. Three alternatively spliced transcript variants of this gene encoding distinct isoforms have been reported.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Product References

CRISPR screens unveil signal hubs for nutrient licensing of T cell immunity.

Nature

Long L,Wei J,Lim SA,Raynor JL,Shi H,Connelly JP,Wang H,Guy C,Xie B,Chapman NM,Fu G,Wang Y,Huang H,Su W,Saravia J,Risch I,Wang YD,Li Y,Niu M,Dhungana Y,Kc A,Zhou P,Vogel P,Yu J,Pruett-Miller SM,Peng J,Chi H

12-5874-82 was used in Flow cytometry/Cell sorting to establish epigenetic and post-translational mechanisms that underpin how nutrient transporters, sensors and transducers interplay with immune signals for three-tiered regulation of mTORC1 activity and identify their pivotal roles in licensing T cell immunity and immune tolerance.

Wed Dec 01 00:00:00 EST 2021

Cyclooxygenase-1 Regulates the Development of Follicular Th Cells via Prostaglandin E₂.

Journal of immunology (Baltimore, Md. : 1950)

Liu T,Yang Q,Cao YJ,Yuan WM,Lei AH,Zhou P,Zhou W,Liu YD,Shi MH,Yang Q,Tang JY,Wang HK,Zhang H,Yu Y,Zhou J

12-5874-82 was used in Flow Cytometry to indicate that COX-1 plays an important role in the development of TFH cells.

Thu Aug 15 00:00:00 EDT 2019

Interleukin-27 Is Essential for Type 1 Diabetes Development and Sjögren Syndrome-like Inflammation.

Cell reports

Ciecko AE,Foda B,Barr JY,Ramanathan S,Atkinson MA,Serreze DV,Geurts AM,Lieberman SM,Chen YG

12-5874-82 was used in Flow Cytometry to reveal that IL-27 contributes to autoimmunity in NOD mice through multiple mechanisms and provides substantial evidence to support its pathogenic role in human T1D.

Tue Dec 03 00:00:00 EST 2019

PD-1 Blockade and CD27 Stimulation Activate Distinct Transcriptional Programs That Synergize for CD8⁺ T-Cell-Driven Antitumor Immunity.

Clinical cancer research : an official journal of the American Association for Cancer Research

Buchan SL,Fallatah M,Thirdborough SM,Taraban VY,Rogel A,Thomas LJ,Penfold CA,He LZ,Curran MA,Keler T,Al-Shamkhani A

12-5874-82 was used in Flow Cytometry to show that suboptimal T-cell invigoration in cancer patients undergoing treatment with PD-1 checkpoint blockers will be improved by dual PD-1 blockade and CD27 agonism.

Tue May 15 00:00:00 EDT 2018

Interleukin-21 promotes germinal center reaction by skewing the follicular regulatory T cell to follicular helper T cell balance in autoimmune BXD2 mice.

Arthritis & rheumatology (Hoboken, N.J.)

Ding Y,Li J,Yang P,Luo B,Wu Q,Zajac AJ,Wildner O,Hsu HC,Mountz JD

12-5874 was used in Flow cytometry/Cell sorting to indicate that high levels of IL-21 selectively enhance Tfh cell differentiation but inhibit Tfr cell commitment and the suppressive function of Tfr cells on Tfh cells and B cells.

Mon Sep 01 00:00:00 EDT 2014

Pegylated bisacycloxypropylcysteine, a diacylated lipopeptide ligand of TLR6, plays a host-protective role against experimental Leishmania major infection.

Journal of immunology (Baltimore, Md. : 1950)

Pandey SP,Chandel HS,Srivastava S,Selvaraj S,Jha MK,Shukla D,Ebensen T,Guzman CA,Saha B

12-5874 was used in Flow cytometry/Cell sorting to investigate the functional differences in different TLR heterodimers.

Wed Oct 01 00:00:00 EDT 2014

Heme oxygenase-1 protects regulatory T cells from hypoxia-induced cellular stress in an experimental mouse brain tumor model.

Journal of neuroimmunology

Dey M,Chang AL,Wainwright DA,Ahmed AU,Han Y,Balyasnikova IV,Lesniak MS

12-5874 was used in Flow cytometry/Cell sorting to assess the benefit of higher HO1 expression in Tregs in glioma.

Wed Jan 15 00:00:00 EST 2014

Alterations in regulatory T cells induced by specific oligosaccharides improve vaccine responsiveness in mice.

PloS one

Schijf MA,Kerperien J,Bastiaans J,Szklany K,Meerding J,Hofman G,Boon L,van Wijk F,Garssen J,Van't Land B

12-5874 was used in Flow cytometry/Cell sorting to identify factors affecting the quality of the immune response to vaccination.

Tue May 27 00:00:00 EDT 2014

Impact of enzymatic tissue disintegration on the level of surface molecule expression and immune cell function.

European journal of microbiology & immunology

Autengruber A,Gereke M,Hansen G,Hennig C,Bruder D

12-5874 was used in Flow cytometry/Cell sorting to indicate that enzymatic tissue disintegration can have profound effects on the expression of a variety of cell-surface molecules.

Fri Jun 01 00:00:00 EDT 2012

CD40 expression levels modulate regulatory T cells in Leishmania donovani infection.

Journal of immunology (Baltimore, Md. : 1950)

Martin S,Agarwal R,Murugaiyan G,Saha B

12-5874 was used in Flow cytometry/Cell sorting to investigate the role of dendritic cell-expressed CD40 in the generation of Tregs.

Thu Jul 01 00:00:00 EDT 2010

Erbb2 DNA vaccine combined with regulatory T cell deletion enhances antibody response and reveals latent low-avidity T cells: potential and limits of its therapeutic efficacy.

Journal of immunology (Baltimore, Md. : 1950)

Rolla S,Ria F,Occhipinti S,Di Sante G,Iezzi M,Spadaro M,Nicolò C,Ambrosino E,Merighi IF,Musiani P,Forni G,Cavallo F

12-5874 was used in Flow cytometry/Cell sorting to assess the therapeutic efficacy of a combination anti-rErbb2 vaccine treatment for blocking tumour progression.

Tue Jun 01 00:00:00 EDT 2010

IL-2-deprivation and TGF-beta are two non-redundant suppressor mechanisms of CD4+CD25+ regulatory T cell which jointly restrain CD4+CD25- cell activation.

Immunology letters

Wang G,Khattar M,Guo Z,Miyahara Y,Linkes SP,Sun Z,He X,Stepkowski SM,Chen W

12-5874 was used in Flow cytometry/Cell sorting to demonstrate that IL-2-deprivation conditions create a unique environment for efficient TGF-β-mediated suppression by Tregs.

Mon Aug 16 00:00:00 EDT 2010

Glutamic acid decarboxylase-derived epitopes with specific domains expand CD4(+)CD25(+) regulatory T cells.

PloS one

Chen G,Han G,Feng J,Wang J,Wang R,Xu R,Shen B,Qian J,Li Y

12-5874 was used in Flow cytometry/Cell sorting to investigate how structural analysis of epitopes may aid predictions of protective epitopes to expand functional Tregs.

Sun Sep 13 00:00:00 EDT 2009

Dicer-dependent microRNA pathway safeguards regulatory T cell function.

The Journal of experimental medicine

Liston A,Lu LF,O'Carroll D,Tarakhovsky A,Rudensky AY

12-5874 was used in Flow cytometry/Cell sorting to study regulatory T cell-mediated tolerance, showing that it is dependent on the Dicer-controlled microRNA pathway.

Mon Sep 01 00:00:00 EDT 2008

TNF-alpha antagonism generates a population of antigen-specific CD4+CD25+ T cells that inhibit protective immunity in murine histoplasmosis.

Journal of immunology (Baltimore, Md. : 1950)

Deepe GS,Gibbons RS

12-5874 was used in Flow cytometry/Cell sorting to investigate whether neutralisation of TNF-alpha triggers the emergence of T cells that dampen immunity.

Tue Jan 15 00:00:00 EST 2008

Immunosurveillance of Erbb2 carcinogenesis in transgenic mice is concealed by a dominant regulatory T-cell self-tolerance.

Cancer research

Ambrosino E,Spadaro M,Iezzi M,Curcio C,Forni G,Musiani P,Wei WZ,Cavallo F

12-5874 was used in Flow cytometry/Cell sorting to investigate role of CD4(+)CD25(+)Foxp3(+) Tregs in overcoming immunosurveillance of Erbb2 mammary lesions, showing that immunosurveillance is concealed by a dominant regulatory T-cell self-tolerance.

Tue Aug 01 00:00:00 EDT 2006

Bioinformatics

Protein Aliases: CD357; Glucocorticoid-induced TNFR-related protein; PRO364; Tumor necrosis factor receptor superfamily member 18; UNQ319

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Gene Aliases: AITR; Gitr; Tnfrsf18

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UniProt ID: (Mouse) O35714

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Entrez Gene ID: (Mouse) 21936

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Function(s)

protein binding transmembrane signal receptor

Process(es)

positive regulation of leukocyte migration apoptotic process positive regulation of tyrosine phosphorylation of Stat1 protein positive regulation of cell adhesion

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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CD357 (AITR/GITR) Monoclonal Antibody (DTA-1), PE, eBioscience™

CD357 (AITR/GITR) Antibody (12-5874-82) in Flow

Product Details

12-5874-82

Flow Cytometry (Flow)

Species Reactivity

Published species

Host/Isotype

Recommended Isotype Control

Class

Type

Clone

Conjugate

Excitation/Emission Max

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

How to use the Panel Builder

Bioinformatics

Performance Guarantee

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