Invitrogen

CD314 (NKG2D) Monoclonal Antibody (CX5), PE, eBioscience™

Advanced Verification

This Antibody was verified by Cell treatment to ensure that the antibody binds to the antigen stated.

6 Published Figures

31 References

View all (49) NKG2D (CD314) antibodies

Datasheet

Protocols

Questions & Answers

Datasheet

Protocols

Questions & Answers

CD314 (NKG2D) Antibody in Flow Cytometry (Flow)

FIGURE: 1 / 8

CD314 (NKG2D) Antibody (12-5882-82) in Flow

Staining of mouse splenocytes with Anti-Mouse CD49b (Integrin alpha 2) FITC (Product # 11-5971-82) and 0.125 µg of Rat IgG1 K Isotype Control PE (Product # 12-4301-82) (left) or 0.125 µg of Anti-Mouse CD314 (NKG2D) PE (right). Cells in the lymphocyte gate were used for analysis.

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Product Details

12-5882-82

Applications

Tested Dilution

Publications

Flow Cytometry (Flow)

0.25 µg/test

View 31 publications 31 publications

Product Specifications

Species Reactivity

Mouse

Published species

Mouse

Host/Isotype

Rat / IgG1, kappa

Recommended Isotype Control

Rat IgG1 kappa Isotype Control (eBRG1), PE, eBioscience™

Class

Monoclonal

Type

Antibody

Clone

CX5

Conjugate

PE PE PE

Excitation/Emission Max

565/576 nm View spectra

Form

Liquid

Concentration

0.2 mg/mL

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.2

Contains

0.09% sodium azide

Storage conditions

4° C, store in dark, DO NOT FREEZE!

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_465996

Product Specific Information

Description: The CX5 monoclonal antibody reacts with the mouse NKG2D, a lectin-like molecule expressed on both human and mouse NK cells. Mouse NKG2D binds to retinoic acid-inducible RAE-1 alpha, beta, gamma, delta, epsilon and the minor histocompatibility molecule H60 and has the ability to costimulate multiple NK activation receptors, through the DAP12/DAP10 adaptor molecules. NKG2D is expressed by all spleen and liver NK cells, NK1.1^+ thymocytes, in vitro activated LAK cells, and a subset of splenic NKT cells.

Applications Reported: The CX5 antibody has been reported for use in flow cytometric analysis.

Applications Tested: The CX5 antibody has been tested by flow cytometric analysis of mouse splenocytes. This can be used at less than or equal to 0.25 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

Excitation: 488-561 nm; Emission: 578 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser.

Filtration: 0.2 µm post-manufacturing filtered.

Target Information

Natural killer (NK) cells are lymphocytes that can mediate lysis of certain tumor cells and virus-infected cells without previous activation. NK cells can regulate specific humoral and cell-mediated immunity, and preferentially express several calcium-dependent (C-type) lectins, which have been implicated in the regulation of NK cell function. The NK gene encodes a member of the NKG2 family, and the encoded transmembrane protein is characterized by a type II membrane orientation (extracellular C terminus) and the presence of a C-type lectin domain. The NKG2 gene family is located within the NK complex, a region that contains several C-type lectin genes preferentially expressed in NK cells.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Product References

Regulatory T cells suppress the formation of potent KLRK1 and IL-7R expressing effector CD8 T cells by limiting IL-2.

eLife

Tsyklauri O,Chadimova T,Niederlova V,Kovarova J,Michalik J,Malatova I,Janusova S,Ivashchenko O,Rossez H,Drobek A,Vecerova H,Galati V,Kovar M,Stepanek O

Published figure using CD314 (NKG2D) monoclonal antibody (Product # 12-5882-82) in Flow Cytometry

Fri Jan 27 00:00:00 EST 2023

Splenic NKG2D confers resilience versus susceptibility in mice after chronic social defeat stress: beneficial effects of (R)-ketamine.

European archives of psychiatry and clinical neuroscience

Zhang K,Sakamoto A,Chang L,Qu Y,Wang S,Pu Y,Tan Y,Wang X,Fujita Y,Ishima T,Hatano M,Hashimoto K

Published figure using CD314 (NKG2D) monoclonal antibody (Product # 12-5882-82) in Flow Cytometry

Thu Apr 01 00:00:00 EDT 2021

Fasudil prevents liver fibrosis via activating natural killer cells and suppressing hepatic stellate cells.

World journal of gastroenterology

Han QJ,Mu YL,Zhao HJ,Zhao RR,Guo QJ,Su YH,Zhang J

12-5882-82 was used in Flow cytometry/Cell sorting to evaluate the anti-fibrotic effects of Fasudil in a mouse model of liver fibrosis induced by thioacetamide.

Mon Jun 28 00:00:00 EDT 2021

Fasudil prevents liver fibrosis via activating natural killer cells and suppressing hepatic stellate cells.

World journal of gastroenterology

Han QJ,Mu YL,Zhao HJ,Zhao RR,Guo QJ,Su YH,Zhang J

12-5882-82 was used in Flow cytometry/Cell sorting to evaluate the anti-fibrotic effects of Fasudil in a mouse model of liver fibrosis induced by thioacetamide.

Mon Jun 28 00:00:00 EDT 2021

Expansion of an Unusual Virtual Memory CD8⁺ Subpopulation Bearing Vα3.2 TCR in Themis-Deficient Mice.

Frontiers in immunology

Prasad M,Wojciech L,Brzostek J,Hu J,Chua YL,Tung DWH,Yap J,Rybakin V,Gascoigne NRJ

Published figure using CD314 (NKG2D) monoclonal antibody (Product # 12-5882-82) in Flow Cytometry

Thu Sep 23 00:00:00 EDT 2021

Synthesis and use of an amphiphilic dendrimer for siRNA delivery into primary immune cells.

Nature protocols

Chen J,Ellert-Miklaszewska A,Garofalo S,Dey AK,Tang J,Jiang Y,Clément F,Marche PN,Liu X,Kaminska B,Santoni A,Limatola C,Rossi JJ,Zhou J,Peng L

12-5882-82 was used in Flow cytometry/Cell sorting to provide here a protocol for the synthesis of this dendrimer, as well as siRNA delivery to immune cells such as primary T and B cells, natural killer cells, macrophages, and primary microglia.

Fri Jan 01 00:00:00 EST 2021

Control of Viral Infection by Natural Killer Cell Inhibitory Receptors.

Cell reports

Parikh BA,Bern MD,Piersma SJ,Yang L,Beckman DL,Poursine-Laurent J,Plougastel-Douglas B,Yokoyama WM

12-5882 was used in Flow cytometry/Cell sorting to show MHC-I-restricted control of murine cytomegalovirus (MCMV) infection in vivo that is NK cell dependent.

Tue Jul 28 00:00:00 EDT 2020

DKK2 imparts tumor immunity evasion through β-catenin-independent suppression of cytotoxic immune-cell activation.

Nature medicine

Xiao Q,Wu J,Wang WJ,Chen S,Zheng Y,Yu X,Meeth K,Sahraei M,Bothwell ALM,Chen L,Bosenberg M,Chen J,Sexl V,Sun L,Li L,Tang W,Wu D

Published figure using CD314 (NKG2D) monoclonal antibody (Product # 12-5882-82) in Flow Cytometry

Thu Mar 01 00:00:00 EST 2018

Loss of HIF-1α in natural killer cells inhibits tumour growth by stimulating non-productive angiogenesis.

Nature communications

Krzywinska E,Kantari-Mimoun C,Kerdiles Y,Sobecki M,Isagawa T,Gotthardt D,Castells M,Haubold J,Millien C,Viel T,Tavitian B,Takeda N,Fandrey J,Vivier E,Sexl V,Stockmann C

12-5882 was used in Flow cytometry/Cell sorting to investigate the mechanisms by which loss of HIF1α in natural killer cells inhibits tumour growth.

Fri Nov 17 00:00:00 EST 2017

The breast tumor microenvironment alters the phenotype and function of natural killer cells.

Cellular & molecular immunology

Krneta T,Gillgrass A,Chew M,Ashkar AA

12-5882 was used in Flow cytometry/Cell sorting to investigate the developmental stage of tumour-associated NK cells, showing that the tumour microenvironment alters the phenotype and function of NK cells.

Thu Sep 01 00:00:00 EDT 2016

Intestinal microbiota modulates gluten-induced immunopathology in humanized mice.

The American journal of pathology

Galipeau HJ,McCarville JL,Huebener S,Litwin O,Meisel M,Jabri B,Sanz Y,Murray JA,Jordana M,Alaedini A,Chirdo FG,Verdu EF

12-5882 was used in Flow cytometry/Cell sorting to assess whether specific microbiota compositions influence immune responses to gluten in mice expressing the human DQ8 gene, which confers moderate CD genetic susceptibility.

Sun Nov 01 00:00:00 EDT 2015

Alopecia areata is driven by cytotoxic T lymphocytes and is reversed by JAK inhibition.

Nature medicine

Xing L,Dai Z,Jabbari A,Cerise JE,Higgins CA,Gong W,de Jong A,Harel S,DeStefano GM,Rothman L,Singh P,Petukhova L,Mackay-Wiggan J,Christiano AM,Clynes R

Published figure using CD314 (NKG2D) monoclonal antibody (Product # 12-5882-82) in Flow Cytometry

Mon Sep 01 00:00:00 EDT 2014

Natural killer cell NKG2D and granzyme B are critical for allergic pulmonary inflammation.

The Journal of allergy and clinical immunology

Farhadi N,Lambert L,Triulzi C,Openshaw PJ,Guerra N,Culley FJ

12-5882 was used in Flow cytometry/Cell sorting to investigate the importance of natural killer cells in house dust mite-triggered allergic response.

Sat Mar 01 00:00:00 EST 2014

Viral MHC class I-like molecule allows evasion of NK cell effector responses in vivo.

Journal of immunology (Baltimore, Md. : 1950)

Pyzik M,Dumaine A,Charbonneau B,Fodil-Cornu N,Jonjic S,Vidal SM

12-5882 was used in Flow cytometry/Cell sorting to show that recognition of the viral protein m157 by the inhibitory Ly49C receptor allows escape from natural killer cell control of viral replication.

Mon Dec 15 00:00:00 EST 2014

Perturbation of NK cell peripheral homeostasis accelerates prostate carcinoma metastasis.

The Journal of clinical investigation

Liu G,Lu S,Wang X,Page ST,Higano CS,Plymate SR,Greenberg NM,Sun S,Li Z,Wu JD

12-5882 was used in Flow cytometry/Cell sorting to provide evidence in vivo that soluble and membrane-restricted NKG2D ligands pose opposite impacts on cancer progression.

Tue Oct 01 00:00:00 EDT 2013

Intratumoral hu14.18-IL-2 (IC) induces local and systemic antitumor effects that involve both activated T and NK cells as well as enhanced IC retention.

Journal of immunology (Baltimore, Md. : 1950)

Yang RK,Kalogriopoulos NA,Rakhmilevich AL,Ranheim EA,Seo S,Kim K,Alderson KL,Gan J,Reisfeld RA,Gillies SD,Hank JA,Sondel PM

12-5882 was used in Flow cytometry/Cell sorting to study how IT-IC resulted in increased numbers of activated T and NK cells within tumours, better IC retention in the tumour, enhanced inhibition of tumour growth, and improved survival compared with i.v.-IC.

Sat Sep 01 00:00:00 EDT 2012

Augmentation of natural cytotoxicity by chronic low-dose ionizing radiation in murine natural killer cells primed by IL-2.

Journal of radiation research

Sonn CH,Choi JR,Kim TJ,Yu YB,Kim K,Shin SC,Park GH,Shirakawa T,Kim HS,Lee KM

12-5882 was used in Flow cytometry/Cell sorting to demonstrate how exposure to LDR can lead to subtle immune-enhancing effects on NK cells.

Thu Nov 01 00:00:00 EDT 2012

Cancer immunotherapy using a bispecific NK receptor fusion protein that engages both T cells and tumor cells.

Cancer research

Zhang T,Sentman CL

12-5882 was used in Flow cytometry/Cell sorting to investigate of a new approach to selectively activate a T-cell attack against tumor cells.

Tue Mar 15 00:00:00 EDT 2011

Natural killer cells recognize friend retrovirus-infected erythroid progenitor cells through NKG2D-RAE-1 interactions In Vivo.

Journal of virology

Ogawa T,Tsuji-Kawahara S,Yuasa T,Kinoshita S,Chikaishi T,Takamura S,Matsumura H,Seya T,Saga T,Miyazawa M

12-5882 was used in Flow cytometry/Cell sorting to demonstrate a correlation between the expression of the products of RAE-1 genes in target cells and their susceptibility to killing by NK cells from FV-infected animals.

Wed Jun 01 00:00:00 EDT 2011

Transience in polarization of cytolytic effectors is required for efficient killing and controlled by Cdc42.

Proceedings of the National Academy of Sciences of the United States of America

Sinai P,Nguyen C,Schatzle JD,Wülfing C

12-5882 was used in Flow cytometry/Cell sorting to support the notion that transience in polarization is critical for cytolytic effector function, likely by preventing cytolytic effectors from becoming trapped in nonproductive target cell interactions.

Tue Jun 29 00:00:00 EDT 2010

Increased killing of liver NK cells by Fas/Fas ligand and NKG2D/NKG2D ligand contributes to hepatocyte necrosis in virus-induced liver failure.

Journal of immunology (Baltimore, Md. : 1950)

Zou Y,Chen T,Han M,Wang H,Yan W,Song G,Wu Z,Wang X,Zhu C,Luo X,Ning Q

12-5882 was used in Flow cytometry/Cell sorting to reveal a pivotal role for liver natural killer cells in the pathogenesis of virus-induced fulminant hepatic failure and acute-on-chronic liver failure.

Fri Jan 01 00:00:00 EST 2010

Effect of polyI:C cotreatment on halothane-induced liver injury in mice.

Hepatology (Baltimore, Md.)

Cheng L,You Q,Yin H,Holt M,Franklin C,Ju C

12-5882 was used in Flow cytometry/Cell sorting to investigate the effect of viral infection on halothane-induced hepatotoxicity in mice using a viral RNA mimetic.

Thu Jan 01 00:00:00 EST 2009

IL-15 is critical for the maintenance and innate functions of self-specific CD8(+) T cells.

European journal of immunology

Itsumi M,Yoshikai Y,Yamada H

12-5882 was used in Flow cytometry/Cell sorting to study the role of memory phenotype CD8+ cells in host defence mechanisms and their origin and antigen specificity.

Wed Jul 01 00:00:00 EDT 2009

Natural killer cells modulate overt autoimmunity to homeostasis in nonobese diabetic mice after anti-CD3 F(ab')2 antibody treatment through secreting transforming growth factor-beta.

The American journal of pathology

Chen G,Han G,Wang J,Wang R,Xu R,Shen B,Qian J,Li Y

12-5882 was used in Flow cytometry/Cell sorting to investigate the mechanisms underlying the use of anti-CD3 for treatment of overt autoimmunity, showing that natural killer cells modulate autoimmunity through secreting TGF-beta.

Tue Sep 01 00:00:00 EDT 2009

MHC class I Dk locus and Ly49G2+ NK cells confer H-2k resistance to murine cytomegalovirus.

Journal of immunology (Baltimore, Md. : 1950)

Xie X,Stadnisky MD,Brown MG

12-5882 was used in Flow cytometry/Cell sorting to investigate resistance to murine cytomegalovirus, showing that the MHC class I Dk locus and Ly49G2+ NK cells confer H-2k resistance.

Mon Jun 01 00:00:00 EDT 2009

Evidence for differential roles for NKG2D receptor signaling in innate host defense against coronavirus-induced neurological and liver disease.

Journal of virology

Walsh KB,Lodoen MB,Edwards RA,Lanier LL,Lane TE

12-5882 was used in Flow cytometry/Cell sorting to investigate the underlying mechanisms associated with protection from disease in MHV-CXCL10-infected mice.

Sat Mar 01 00:00:00 EST 2008

Mechanisms involved in synergistic anticancer immunity of anti-4-1BB and anti-CD4 therapy.

Cancer research

Choi BK,Kim YH,Kang WJ,Lee SK,Kim KH,Shin SM,Yokoyama WM,Kim TY,Kwon BS

12-5882 was used in Flow cytometry/Cell sorting to demonstrate a therapy that combines the use of two mAbs for the enhanced production of efficient tumour-killing cytotoxic T lymphocytes.

Sat Sep 15 00:00:00 EDT 2007

Blockade of NKG2D on NKT cells prevents hepatitis and the acute immune response to hepatitis B virus.

Proceedings of the National Academy of Sciences of the United States of America

Vilarinho S,Ogasawara K,Nishimura S,Lanier LL,Baron JL

12-5882 was used in Flow cytometry/Cell sorting to demonstrate that surface expression of NKG2D and its ligand RAE-1 are modulated in an Hepatitis B virus-dependent manner.

Tue Nov 13 00:00:00 EST 2007

Natural killer cell education in mice with single or multiple major histocompatibility complex class I molecules.

The Journal of experimental medicine

Johansson S,Johansson M,Rosmaraki E,Vahlne G,Mehr R,Salmon-Divon M,Lemonnier F,Kärre K,Höglund P

12-5882 was used in Flow cytometry/Cell sorting to indicate that natural killer cell education may be regulated by a combination of qualitative and quantitative events.

Mon Apr 04 00:00:00 EDT 2005

Suppression of tumor formation in lymph nodes by L-selectin-mediated natural killer cell recruitment.

The Journal of experimental medicine

Chen S,Kawashima H,Lowe JB,Lanier LL,Fukuda M

12-5882 was used in Flow cytometry/Cell sorting to indicate that L-selectin-mediated NK cell recruitment plays a crucial role in the control of tumour metastasis into secondary lymphoid organs.

Mon Dec 19 00:00:00 EST 2005

Important roles for gamma interferon and NKG2D in gammadelta T-cell-induced demyelination in T-cell receptor beta-deficient mice infected with a coronavirus.

Journal of virology

Dandekar AA,O'Malley K,Perlman S

12-5882 was used in Flow cytometry/Cell sorting to investigate the role of gammadelta T cells in demyelination in TCRbeta(-/-) mice.

Mon Aug 01 00:00:00 EDT 2005

NKT cell stimulation with glycolipid antigen in vivo: costimulation-dependent expansion, Bim-dependent contraction, and hyporesponsiveness to further antigenic challenge.

Journal of immunology (Baltimore, Md. : 1950)

Uldrich AP,Crowe NY,Kyparissoudis K,Pellicci DG,Zhan Y,Lew AM,Bouillet P,Strasser A,Smyth MJ,Godfrey DI

12-5882 was used in Flow cytometry/Cell sorting to investigate how NKT cells respond to primary and secondary antigenic challenge in vivo.

Thu Sep 01 00:00:00 EDT 2005

Bioinformatics

Protein Aliases: CD314; Killer cell lectin-like receptor subfamily K member 1; natural killer cell group 2D; natural killer cell receptor; NK cell receptor D; NKG2-D type II integral membrane protein; NKG2-D-activating NK receptor

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Gene Aliases: D6H12S2489E; Klrk1; NKG2-D; Nkg2d

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UniProt ID: (Mouse) O54709

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Entrez Gene ID: (Mouse) 27007

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Function(s)

protein binding kinase binding carbohydrate binding MHC class Ib receptor activity MHC class I protein binding protein homodimerization activity

Process(es)

stimulatory C-type lectin receptor signaling pathway immune system process positive regulation of natural killer cell mediated cytotoxicity directed against tumor cell target natural killer cell activation cell differentiation positive regulation of myeloid dendritic cell activation positive regulation of interferon-gamma production positive regulation of apoptotic process innate immune response positive regulation of nitric oxide biosynthetic process positive regulation of natural killer cell mediated cytotoxicity defense response to Gram-positive bacterium cellular response to lipopolysaccharide

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CD314 (NKG2D) Monoclonal Antibody (CX5), PE, eBioscience™

CD314 (NKG2D) Antibody (12-5882-82) in Flow

Product Details

12-5882-82

Flow Cytometry (Flow)

Species Reactivity

Published species

Host/Isotype

Recommended Isotype Control

Class

Type

Clone

Conjugate

Excitation/Emission Max

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

How to use the Panel Builder

Bioinformatics

Performance Guarantee

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