Invitrogen

CD314 (NKG2D) Monoclonal Antibody (CX5), APC, eBioscience™

6 Published Figures

22 References

View all (49) NKG2D (CD314) antibodies

Datasheet

Protocols

Questions & Answers

Datasheet

Protocols

Questions & Answers

Antibody Testing Data (1)
Published Figures (6)

CD314 (NKG2D) Antibody in Flow Cytometry (Flow)

FIGURE: 1 / 7

CD314 (NKG2D) Antibody (17-5882-82) in Flow

Staining of mouse splenocytes with Anti-Mouse CD49b (Integrin alpha 2) PE (Product # 12-5971-82) and 1.0 µg of Rat IgG1 K Isotype Control APC (Product # 17-4301-82) (left) or 1.0 µg of Anti-Mouse CD314 (NKG2D) APC (right). Cells in the lymphocyte gate were used for the analysis.

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Product Details

17-5882-82

Applications

Tested Dilution

Publications

Flow Cytometry (Flow)

1 µg/test

View 22 publications 22 publications

Product Specifications

Species Reactivity

Mouse

Published species

Human, Mouse

Host/Isotype

Rat / IgG1, kappa

Recommended Isotype Control

Rat IgG1 kappa Isotype Control (eBRG1), APC, eBioscience™

Class

Monoclonal

Type

Antibody

Clone

CX5

Conjugate

APC APC APC

Excitation/Emission Max

651/660 nm View spectra

Form

Liquid

Concentration

0.2 mg/mL

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.2

Contains

0.09% sodium azide

Storage conditions

4° C, store in dark, DO NOT FREEZE!

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_469464

Product Specific Information

Description: The CX5 monoclonal antibody reacts with the mouse NKG2D, a lectin-like molecule expressed on both human and mouse NK cells. Mouse NKG2D binds to retinoic acid-inducible RAE-1 alpha, beta, gamma, delta, epsilon and the minor histocompatibility molecule H60 and has the ability to costimulate multiple NK activation receptors, through the DAP12/DAP10 adaptor molecules. NKG2D is expressed by all spleen and liver NK cells, NK1.1^+ thymocytes, in vitro activated LAK cells, and a subset of splenic NKT cells.

Applications Reported: This CX5 antibody has been reported for use in flow cytometric analysis.

Applications Tested: This CX5 antibody has been tested by flow cytometric analysis of mouse splenocytes. This can be used at less than or equal to 1 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

Excitation: 633-647 nm; Emission: 660 nm; Laser: Red Laser.

Filtration: 0.2 µm post-manufacturing filtered.

Target Information

Natural killer (NK) cells are lymphocytes that can mediate lysis of certain tumor cells and virus-infected cells without previous activation. NK cells can regulate specific humoral and cell-mediated immunity, and preferentially express several calcium-dependent (C-type) lectins, which have been implicated in the regulation of NK cell function. The NK gene encodes a member of the NKG2 family, and the encoded transmembrane protein is characterized by a type II membrane orientation (extracellular C terminus) and the presence of a C-type lectin domain. The NKG2 gene family is located within the NK complex, a region that contains several C-type lectin genes preferentially expressed in NK cells.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Product References

Regulatory T cells suppress the formation of potent KLRK1 and IL-7R expressing effector CD8 T cells by limiting IL-2.

eLife

Tsyklauri O,Chadimova T,Niederlova V,Kovarova J,Michalik J,Malatova I,Janusova S,Ivashchenko O,Rossez H,Drobek A,Vecerova H,Galati V,Kovar M,Stepanek O

Published figure using CD314 (NKG2D) monoclonal antibody (Product # 17-5882-82) in Flow Cytometry

Fri Jan 27 00:00:00 EST 2023

Splenic NKG2D confers resilience versus susceptibility in mice after chronic social defeat stress: beneficial effects of (R)-ketamine.

European archives of psychiatry and clinical neuroscience

Zhang K,Sakamoto A,Chang L,Qu Y,Wang S,Pu Y,Tan Y,Wang X,Fujita Y,Ishima T,Hatano M,Hashimoto K

Published figure using CD314 (NKG2D) monoclonal antibody (Product # 17-5882-82) in Flow Cytometry

Thu Apr 01 00:00:00 EDT 2021

Fasudil prevents liver fibrosis via activating natural killer cells and suppressing hepatic stellate cells.

World journal of gastroenterology

Han QJ,Mu YL,Zhao HJ,Zhao RR,Guo QJ,Su YH,Zhang J

Published figure using CD314 (NKG2D) monoclonal antibody (Product # 17-5882-82) in Flow Cytometry

Mon Jun 28 00:00:00 EDT 2021

Expansion of an Unusual Virtual Memory CD8⁺ Subpopulation Bearing Vα3.2 TCR in Themis-Deficient Mice.

Frontiers in immunology

Prasad M,Wojciech L,Brzostek J,Hu J,Chua YL,Tung DWH,Yap J,Rybakin V,Gascoigne NRJ

Published figure using CD314 (NKG2D) monoclonal antibody (Product # 17-5882-82) in Flow Cytometry

Thu Sep 23 00:00:00 EDT 2021

DKK2 imparts tumor immunity evasion through β-catenin-independent suppression of cytotoxic immune-cell activation.

Nature medicine

Xiao Q,Wu J,Wang WJ,Chen S,Zheng Y,Yu X,Meeth K,Sahraei M,Bothwell ALM,Chen L,Bosenberg M,Chen J,Sexl V,Sun L,Li L,Tang W,Wu D

Published figure using CD314 (NKG2D) monoclonal antibody (Product # 17-5882-82) in Flow Cytometry

Thu Mar 01 00:00:00 EST 2018

Dysfunction of Natural Killer Cells by FBP1-Induced Inhibition of Glycolysis during Lung Cancer Progression.

Cell metabolism

Cong J,Wang X,Zheng X,Wang D,Fu B,Sun R,Tian Z,Wei H

17-5882 was used in Flow cytometry/Cell sorting to identify novel mechanisms in natural killer cell dysfunction.

Tue Aug 07 00:00:00 EDT 2018

The breast tumor microenvironment alters the phenotype and function of natural killer cells.

Cellular & molecular immunology

Krneta T,Gillgrass A,Chew M,Ashkar AA

Published figure using CD314 (NKG2D) monoclonal antibody (Product # 17-5882-82) in Flow Cytometry

Thu Sep 01 00:00:00 EDT 2016

Diacylglycerol Kinase ζ Is a Target To Enhance NK Cell Function.

Journal of immunology (Baltimore, Md. : 1950)

Yang E,Singh BK,Paustian AM,Kambayashi T

17-5882 was used in Flow cytometry/Cell sorting to investigate methods to enhance NK cell function, showing that diacylglycerol kinase ζ is a target for enhancement of NK function.

Mon Aug 01 00:00:00 EDT 2016

Taxanes enhance trastuzumab-mediated ADCC on tumor cells through NKG2D-mediated NK cell recognition.

Oncotarget

Di Modica M,Sfondrini L,Regondi V,Varchetta S,Oliviero B,Mariani G,Bianchi GV,Generali D,Balsari A,Triulzi T,Tagliabue E

17-5882 was used in Flow cytometry/Cell sorting to indicate that taxanes increase tumor susceptibility to ADCC by acting on tumor and NK cells.

Tue Jan 05 00:00:00 EST 2016

Increased serum NKG2D-ligands and downregulation of NKG2D in peripheral blood NK cells of patients with major burns.

Oncotarget

Haik J,Nardini G,Goldman N,Galore-Haskel G,Harats M,Zilinsky I,Weissman O,Schachter J,Winkler E,Markel G

17-5882 was used in Flow cytometry/Cell sorting to comparatively study the process and recovery potential of cohorts of patients with Major and Non-Major Burns as well as healthy donors.

Tue Jan 19 00:00:00 EST 2016

Toll-Like receptor 2 (TLR2) and TLR9 play opposing roles in host innate immunity against Salmonella enterica serovar Typhimurium infection.

Infection and immunity

Zhan R,Han Q,Zhang C,Tian Z,Zhang J

17-5882 was used in Flow cytometry/Cell sorting to investigate the roles of TLR2 and TLR9 in regulating innate immunity against oral infection of Salmonella enterica serovar Typhimurium in mice.

Wed Apr 01 00:00:00 EDT 2015

Alopecia areata is driven by cytotoxic T lymphocytes and is reversed by JAK inhibition.

Nature medicine

Xing L,Dai Z,Jabbari A,Cerise JE,Higgins CA,Gong W,de Jong A,Harel S,DeStefano GM,Rothman L,Singh P,Petukhova L,Mackay-Wiggan J,Christiano AM,Clynes R

Published figure using CD314 (NKG2D) monoclonal antibody (Product # 17-5882-82) in Flow Cytometry

Mon Sep 01 00:00:00 EDT 2014

NK cells impede glioblastoma virotherapy through NKp30 and NKp46 natural cytotoxicity receptors.

Nature medicine

Alvarez-Breckenridge CA,Yu J,Price R,Wojton J,Pradarelli J,Mao H,Wei M,Wang Y,He S,Hardcastle J,Fernandez SA,Kaur B,Lawler SE,Vivier E,Mandelboim O,Moretta A,Caligiuri MA,Chiocca EA

17-5882 was used in Flow cytometry/Cell sorting to demonstrate that glioblastoma virotherapy is limited partially by an antiviral NK cell response involving specific NCRs, uncovering new potential targets to enhance cancer virotherapy.

Sat Dec 01 00:00:00 EST 2012

Histone deacetylase inhibitors impair NK cell viability and effector functions through inhibition of activation and receptor expression.

Journal of leukocyte biology

Rossi LE,Avila DE,Spallanzani RG,Ziblat A,Fuertes MB,Lapyckyj L,Croci DO,Rabinovich GA,Domaica CI,Zwirner NW

17-5882 was used in Flow cytometry/Cell sorting to investigate the effect of histone deacetylase inhibitors on immune cells, showing that they can impair NK cell viability and effector functions.

Wed Feb 01 00:00:00 EST 2012

Small intestinal intraepithelial lymphocytes expressing CD8 and T cell receptor γδ are involved in bacterial clearance during Salmonella enterica serovar Typhimurium infection.

Infection and immunity

Li Z,Zhang C,Zhou Z,Zhang J,Zhang J,Tian Z

17-5882 was used in Flow cytometry/Cell sorting to investigate the role of intestinal intraepithelial lymphocytes in responding to oral infection, showing that they express CD8 and T cell receptor γδ for bacterial clearance.

Wed Feb 01 00:00:00 EST 2012

Myeloid-derived suppressor cells are implicated in regulating permissiveness for tumor metastasis during mouse gestation.

The Journal of clinical investigation

Mauti LA,Le Bitoux MA,Baumer K,Stehle JC,Golshayan D,Provero P,Stamenkovic I

17-5882 was used in Flow cytometry/Cell sorting to suggest that the functions of MDSCs may represent a shared mechanism of immunosuppression that occurs during gestation and tumour growth.

Fri Jul 01 00:00:00 EDT 2011

Regulatory role of Vγ1 γδ T cells in tumor immunity through IL-4 production.

Journal of immunology (Baltimore, Md. : 1950)

Hao J,Dong S,Xia S,He W,Jia H,Zhang S,Wei J,O'Brien RL,Born WK,Wu Z,Wang P,Han J,Hong Z,Zhao L,Yin Z

17-5882 was used in Flow cytometry/Cell sorting to establish a negative regulatory role of Vγ1 γδ T cells in Vγ4 γδ T cell-mediated antitumor immunity through cell contact-independent and IL-4-mediated mechanisms.

Tue Nov 15 00:00:00 EST 2011

NKG2D receptor signaling enhances cytolytic activity by virus-specific CD8+ T cells: evidence for a protective role in virus-induced encephalitis.

Journal of virology

Walsh KB,Lanier LL,Lane TE

17-5882 was used in Flow cytometry/Cell sorting to elucidate the functional contributions of the activating NKG2D receptor in host defense and disease following JHMV infection.

Sat Mar 01 00:00:00 EST 2008

The immune evasion paradox: immunoevasins of murine cytomegalovirus enhance priming of CD8 T cells by preventing negative feedback regulation.

Journal of virology

Böhm V,Simon CO,Podlech J,Seckert CK,Gendig D,Deegen P,Gillert-Marien D,Lemmermann NA,Holtappels R,Reddehase MJ

17-5882 was used in Flow cytometry/Cell sorting to support the model that immunoevasins improve antigen supply for indirect priming by uninfected antigen-presenting cells.

Mon Dec 01 00:00:00 EST 2008

NKG2D is critical for NK cell activation in host defense against Pseudomonas aeruginosa respiratory infection.

Journal of immunology (Baltimore, Md. : 1950)

Wesselkamper SC,Eppert BL,Motz GT,Lau GW,Hassett DJ,Borchers MT

17-5882 was used in Flow cytometry/Cell sorting to investigate the cellular and molecular mechanisms of NKG2D-mediated clearance of P. aeruginosa.

Wed Oct 15 00:00:00 EDT 2008

Unique receptor repertoire in mouse uterine NK cells.

Journal of immunology (Baltimore, Md. : 1950)

Yadi H,Burke S,Madeja Z,Hemberger M,Moffett A,Colucci F

17-5882-82 was used in Flow Cytometry to define the receptor repertoire and phenotype of uterine NK cells at mid-gestation in mice.

Sat Nov 01 00:00:00 EDT 2008

Zoonotic orthopoxviruses encode a high-affinity antagonist of NKG2D.

The Journal of experimental medicine

Campbell JA,Trossman DS,Yokoyama WM,Carayannopoulos LN

17-5882 was used in Flow cytometry/Cell sorting to investigate how viruses interfere with the function of major histocompatibility complex class I superfamily (MHCISF) receptors to evade immunologic cytotoxicity, showing that zoonotic orthopoxviruses encode a high-affinity antagonist of NKG2D.

Mon Jun 11 00:00:00 EDT 2007

Bioinformatics

Protein Aliases: CD314; Killer cell lectin-like receptor subfamily K member 1; natural killer cell group 2D; natural killer cell receptor; NK cell receptor D; NKG2-D type II integral membrane protein; NKG2-D-activating NK receptor

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Gene Aliases: D6H12S2489E; Klrk1; NKG2-D; Nkg2d

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UniProt ID: (Mouse) O54709

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Entrez Gene ID: (Mouse) 27007

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Function(s)

protein binding kinase binding carbohydrate binding MHC class Ib receptor activity MHC class I protein binding protein homodimerization activity

Process(es)

stimulatory C-type lectin receptor signaling pathway immune system process positive regulation of natural killer cell mediated cytotoxicity directed against tumor cell target natural killer cell activation cell differentiation positive regulation of myeloid dendritic cell activation positive regulation of interferon-gamma production positive regulation of apoptotic process innate immune response positive regulation of nitric oxide biosynthetic process positive regulation of natural killer cell mediated cytotoxicity defense response to Gram-positive bacterium cellular response to lipopolysaccharide

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CD314 (NKG2D) Monoclonal Antibody (CX5), APC, eBioscience™

CD314 (NKG2D) Antibody (17-5882-82) in Flow

Product Details

17-5882-82

Flow Cytometry (Flow)

Species Reactivity

Published species

Host/Isotype

Recommended Isotype Control

Class

Type

Clone

Conjugate

Excitation/Emission Max

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

How to use the Panel Builder

Bioinformatics

Performance Guarantee

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