Invitrogen

Phospho-S6 (Ser235, Ser236) Monoclonal Antibody (cupk43k), PE, eBioscience™

Phospho-S6 (Ser235, Ser236) Antibody in Flow Cytometry (Flow)

FIGURE: 1 / 1

Phospho-S6 (Ser235, Ser236) Antibody (12-9007-42) in Flow

Intracellular staining of freshly-harvested (left), unstimulated (middle), or 30-minute LPS-stimulated (right) normal human peripheral blood cells with Anti-Human CD14 FITC (Product # 11-0149-42) and Anti-Human phospho-S6 (S235/S236) PE, using the Intracellular Fixation and Permeabilization Buffer Set (Product # 88-8824-00) and protocol. Cells in the lymphocyte/monocyte gate were used for analysis.

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Product Details

12-9007-42

Applications

Tested Dilution

Publications

Immunocytochemistry (ICC/IF)

- -

Flow Cytometry (Flow)

5 µL (0.125 µg)/test

View 7 publications 7 publications

Product Specifications

Species Reactivity

Human, Mouse

Published species

Mouse

Host/Isotype

Mouse / IgG1, kappa

Recommended Isotype Control

Mouse IgG1 kappa Isotype Control (P3.6.2.8.1), PE, eBioscience™

Class

Monoclonal

Type

Antibody

Clone

cupk43k

Conjugate

PE PE PE

Excitation/Emission Max

565/576 nm View spectra

Form

Liquid

Concentration

5 µL/Test

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.2, with 0.2% BSA

Contains

0.09% sodium azide

Storage conditions

4° C, store in dark, DO NOT FREEZE!

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2572667

Product Specific Information

Description: This cupk43k monoclonal antibody recognizes human and mouse ribosomal protein S6 (also known as 40S ribosomal protein S6, phosphoprotein NP33, RPS6, RS6, S6) when phosphorylated on serine 235 (S235, human) and serine 236 (S236, mouse). Ribosomal protein S6 is a component of the 40S subunit of the ribosome and is phosphorylated at multiple sites following stimulation of cells by growth factors, tumor promoting agents, or mitogens. Phosphorylation of ribosomal protein S6 by p70S6K and PKDCD results in upregulation of the translation of RNA coding for proteins involved in cell cycle entry. Ribosomal protein S6 is dephosphorylated upon growth arrest.

The specificity of the cupk43k monoclonal antibody was determined by western blotting.

Applications Reported: This cupk43k antibody has been reported for use in intracellular staining followed by flow cytometric analysis.

Applications Tested: This cupk43k antibody has been pre-titrated and tested by intracellular staining and flow cytometric analysis of stimulated normal human peripheral blood cells. This can be used at 5 µL (0.125 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test.

Use of Protocol A: Two-step protocol: intracellular (cytoplasmic) proteins allows for the greatest flexibility for detection of surface and intracellular (cytoplasmic) proteins. Use of Protocol B: One-step protocol: intracellular (nuclear) proteins is recommended for staining of transcription factors in conjunction with surface and phosphorylated intracellular (cytoplasmic) proteins. Protocol C: Two-step protocol: Fixation/Methanol allows for the greatest discrimination of phospho-specific signaling between unstimulated and stimulated samples, but with limitations on the ability to stain specific surface proteins (refer to "Clone Performance Following Fixation/Permeabilization" located in the Best Protocols Section under the Resources tab online). All Protocols can be found in the "Staining Intracellular Antigens for Flow Cytometry Protocol" located in the Best Protocols Section under the Resources tab online.

Excitation: 488-561 nm; Emission: 578 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser.

Filtration: 0.2 µm post-manufacturing filtered.

Target Information

Ribosomes, the organelles that catalyze protein synthesis, consist of a small 40S subunit and a large 60S subunit. Together these subunits are composed of 4 RNA species and approximately 80 structurally distinct proteins. This gene encodes a cytoplasmic ribosomal protein that is a component of the 40S subunit. The protein belongs to the S6E family of ribosomal proteins. It is the major substrate of protein kinases in the ribosome, with subsets of five C-terminal serine residues phosphorylated by different protein kinases. Phosphorylation is induced by a wide range of stimuli, including growth factors, tumor-promoting agents, and mitogens. Dephosphorylation occurs at growth arrest. The protein may contribute to the control of cell growth and proliferation through the selective translation of particular classes of mRNA. As is typical for genes encoding ribosomal proteins, there are multiple processed pseudogenes of this gene dispersed through the genome.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Product References

Extracellular acidosis restricts one-carbon metabolism and preserves T cell stemness.

Nature metabolism

Cheng H,Qiu Y,Xu Y,Chen L,Ma K,Tao M,Frankiw L,Yin H,Xie E,Pan X,Du J,Wang Z,Zhu W,Chen L,Zhang L,Li G

12-9007-42 was used in Flow cytometry/Cell sorting to reveal an unexpected capacity of extracellular acidosis to maintain the stem-like properties of T cells, but also advance our understanding of how methionine metabolism affects T cell stemness.

Wed Feb 01 00:00:00 EST 2023

Cbl Ubiquitin Ligases Control B Cell Exit from the Germinal-Center Reaction.

Immunity

Li X,Gadzinsky A,Gong L,Tong H,Calderon V,Li Y,Kitamura D,Klein U,Langdon WY,Hou F,Zou YR,Gu H

12-9007-42 was used in Flow Cytometry to show that deleting E3 ubiquitin ligases Cbl and Cbl-b (Cbls) in GC B cells results in the early exit of high-affinity antigen-specific B cells from the GC reaction and thus impaired clonal expansion.

Tue Mar 20 00:00:00 EDT 2018

Autophagy orchestrates the regulatory program of tumor-associated myeloid-derived suppressor cells.

The Journal of clinical investigation

Alissafi T,Hatzioannou A,Mintzas K,Barouni RM,Banos A,Sormendi S,Polyzos A,Xilouri M,Wielockx B,Gogas H,Verginis P

12-9007 was used in Flow cytometry/Cell sorting to identify autophagy as a crucial pathway for myeloid-derived suppressor cell-mediated suppression of antitumour immunity.

Fri Aug 31 00:00:00 EDT 2018

Intestinal Epithelial and Intraepithelial T Cell Crosstalk Mediates a Dynamic Response to Infection.

Cell

Hoytema van Konijnenburg DP,Reis BS,Pedicord VA,Farache J,Victora GD,Mucida D

12-9007 was used in Flow cytometry/Cell sorting to investigate the nature of intestinal intraepithelial lymphocyte responses to luminal microbes.

Thu Nov 02 00:00:00 EDT 2017

Dichotomous Expression of TNF Superfamily Ligands on Antigen-Presenting Cells Controls Post-priming Anti-viral CD4⁺ T Cell Immunity.

Immunity

Chang YH,Wang KC,Chu KL,Clouthier DL,Tran AT,Torres Perez MS,Zhou AC,Abdul-Sater AA,Watts TH

12-9007-42 was used in Flow Cytometry to investigate how different subtypes of T cell antigen-presenting cell contribute to determining viral set point and disease outcome.

Tue Nov 21 00:00:00 EST 2017

Osteoblasts support megakaryopoiesis through production of interleukin-9.

Blood

Xiao M,Wang Y,Tao C,Wang Z,Yang J,Chen Z,Zou Z,Li M,Liu A,Jia C,Huang B,Yan B,Lai P,Ding C,Cai D,Xiao G,Jiang Y,Bai X

12-9007-42 was used in Flow Cytometry to indicate that IL-9 is an essential regulator of megakaryopoiesis and a promising therapeutic agent for treatment of thrombocytopenia such as CIT.

Thu Jun 15 00:00:00 EDT 2017

PTEN drives Th17 cell differentiation by preventing IL-2 production.

The Journal of experimental medicine

Kim HS,Jang SW,Lee W,Kim K,Sohn H,Hwang SS,Lee GR

12-9007 was used in Flow cytometry/Cell sorting to show that PTEN plays a key role in Th17 cell differentiation by blocking IL-2 expression.

Mon Nov 06 00:00:00 EST 2017

Bioinformatics

Protein Aliases: 40S ribosomal protein S6; OTTHUMP00000021121; OTTHUMP00000021122; Phosphoprotein NP33; RP11-513M16.6; RS6; Small ribosomal subunit protein eS6

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Gene Aliases: OK/SW-cl.2; RPS6; S6; S6R

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UniProt ID: (Human) P62753, (Mouse) P62754

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Entrez Gene ID: (Human) 6194, (Mouse) 20104

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Function(s)

structural constituent of ribosome protein binding protein kinase binding poly(A) RNA binding ribosomal protein translational protein

Process(es)

G1/S transition of mitotic cell cycle nuclear-transcribed mRNA catabolic process, nonsense-mediated decay placenta development T cell proliferation involved in immune response rRNA processing translation translational initiation SRP-dependent cotranslational protein targeting to membrane activation-induced cell death of T cells mitotic nuclear division mitotic cell cycle checkpoint gastrulation viral transcription oogenesis stage TOR signaling T cell differentiation in thymus ribosomal small subunit biogenesis glucose homeostasis positive regulation of apoptotic process negative regulation of apoptotic process erythrocyte development

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Phospho-S6 (Ser235, Ser236) Monoclonal Antibody (cupk43k), PE, eBioscience™

Phospho-S6 (Ser235, Ser236) Antibody (12-9007-42) in Flow

Product Details

12-9007-42

Immunocytochemistry (ICC/IF)

Flow Cytometry (Flow)

Species Reactivity

Published species

Host/Isotype

Recommended Isotype Control

Class

Type

Clone

Conjugate

Excitation/Emission Max

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

How to use the Panel Builder

Bioinformatics

Performance Guarantee

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