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Invitrogen
Phospho-S6 (Ser235, Ser236) Monoclonal Antibody (cupk43k), eBioscience™
Advanced Verification
This Antibody was verified by Cell treatment to ensure that the antibody binds to the antigen stated.
FIGURE: 1 / 5
Phospho-S6 (Ser235, Ser236) Antibody (14-9007-82) in WB
Western blot was performed using Anti-Phospho-S6 (Ser235, Ser236) Monoclonal Antibody (cupk43k), eBioscience™(Product # 14-9007-82) and a 32 kDa band corresponding to Phospho-S6 (Ser235, Ser236) was observed across the cell lines tested. Whole cell extracts (30 µg lysate) of U-2 OS (Lane 1), U-2 OS treated with IGF (Lane 2), U-2 OS treated with Rapamycin (Lane 3), U-2 OS treated with both IGF and Rapamycin ... View More
Product Details
14-9007-82
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse
Published species
Mouse
Host/Isotype
Mouse
/ IgG1, kappa
Class
Monoclonal
Type
Antibody
Clone
cupk43k
Conjugate
Form
Liquid
Concentration
0.5 mg/mL
Purification
Affinity chromatography
Storage buffer
PBS, pH 7.2
Contains
0.09% sodium azide
Storage conditions
4° C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2572911
Product Specific Information
Description: This cupk43k monoclonal antibody recognizes human and mouse ribosomal protein S6 (also known as 40S ribosomal protein S6, phosphoprotein NP33, RPS6, RS6, S6) when phosphorylated on serine 235 (S235, human) and serine 236 (S236, mouse). Ribosomal protein S6 is a component of the 40S subunit of the ribosome and is phosphorylated at multiple sites following stimulation of cells by growth factors, tumor promoting agents, or mitogens. Phosphorylation of ribosomal protein S6 by p70S6K and PKDCD results in upregulation of the translation of RNA coding for proteins involved in cell cycle entry. Ribosomal protein S6 is dephosphorylated upon growth arrest.
The specificity of the cupk43k monoclonal antibody was determined by western blotting.
Applications Reported: This cupk43k antibody has been reported for use in intracellular staining followed by flow cytometric analysis, and western blotting. (Fluorochrome conjugated cupk43k is recommended for use in intracellular staining and flow cytometric analysis.).
Applications Tested: This cupk43k antibody has been tested by immunoblotting of stimulated normal human peripheral blood cells. This can be used at less than or equal to 5 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.
Purity: Greater than 90%, as determined by SDS-PAGE.
Aggregation: Less than 10%, as determined by HPLC.
Filtration: 0.2 µm post-manufacturing filtered.
Target Information
Ribosomes, the organelles that catalyze protein synthesis, consist of a small 40S subunit and a large 60S subunit. Together these subunits are composed of 4 RNA species and approximately 80 structurally distinct proteins. This gene encodes a cytoplasmic ribosomal protein that is a component of the 40S subunit. The protein belongs to the S6E family of ribosomal proteins. It is the major substrate of protein kinases in the ribosome, with subsets of five C-terminal serine residues phosphorylated by different protein kinases. Phosphorylation is induced by a wide range of stimuli, including growth factors, tumor-promoting agents, and mitogens. Dephosphorylation occurs at growth arrest. The protein may contribute to the control of cell growth and proliferation through the selective translation of particular classes of mRNA. As is typical for genes encoding ribosomal proteins, there are multiple processed pseudogenes of this gene dispersed through the genome.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: 40S ribosomal protein S6; OTTHUMP00000021121; OTTHUMP00000021122; Phosphoprotein NP33; RP11-513M16.6; RS6; Small ribosomal subunit protein eS6
Gene Aliases: OK/SW-cl.2; RPS6; S6; S6R
UniProt ID: (Human) P62753, (Mouse) P62754
Entrez Gene ID: (Human) 6194, (Mouse) 20104
G1/S transition of mitotic cell cycle
nuclear-transcribed mRNA catabolic process, nonsense-mediated decay
placenta development
T cell proliferation involved in immune response
rRNA processing
translation
translational initiation
SRP-dependent cotranslational protein targeting to membrane
activation-induced cell death of T cells
mitotic nuclear division
mitotic cell cycle checkpoint
gastrulation
viral transcription
oogenesis stage
TOR signaling
T cell differentiation in thymus
ribosomal small subunit biogenesis
glucose homeostasis
positive regulation of apoptotic process
negative regulation of apoptotic process
erythrocyte development
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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