Invitrogen

Gata-3 Monoclonal Antibody (TWAJ), PE, eBioscience™

Gata-3 Antibody in Flow Cytometry (Flow)

FIGURE: 1 / 1

Gata-3 Antibody (12-9966-42) in Flow

Staining of CD4 (Product # 11-0041-82) single positive (left) and CD8 (Product # 45-0081-82) single positive (right) BALB/c thymocytes using the Foxp3 Staining Buffer Set (Product # 00-5523-00) with Rat IgG2b K Isotype Control PE (Product # 12-4031-82) (blue histogram) or Anti-Human/Mouse Gata-3 PE (purple histogram).

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Product Details

12-9966-42

Applications

Tested Dilution

Publications

Flow Cytometry (Flow)

5 µL (0.06 µg)/test

View 58 publications 58 publications

Product Specifications

Species Reactivity

Human, Mouse, Pig, Rhesus monkey

Published species

Human, Mouse

Host/Isotype

Rat / IgG2b, kappa

Recommended Isotype Control

Rat IgG2b kappa Isotype Control (eB149/10H5), PE, eBioscience™

Class

Monoclonal

Type

Antibody

Clone

TWAJ

Conjugate

PE PE PE

Excitation/Emission Max

565/576 nm View spectra

Form

Liquid

Concentration

5 µL/Test

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.2, with 0.2% BSA

Contains

0.09% sodium azide

Storage conditions

4° C, store in dark, DO NOT FREEZE!

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_1963600

Product Specific Information

Description: The monoclonal antibody TWAJ recognizes mouse and human Gata-3, a member of the Gata family of transcription factors. Gata-3 is a T cell-specific transcription factor important for thymic development and Th2 differentiation. Expression during embryonic development is found in the central nervous system, skin, mammary glands and kidney. During development, the expression of Gata-3 is essential as homozygous knock-out of Gata-3 is embryonic lethal. The Gata-3 is also essential for T cell commitment and survival. In the thymus, expression is found mainly on the CD4 single positive cells. During Th2 differentiation, Gata-3 binds to the IL-4 promoter as well as represses the expression of T-bet, thus inhibiting Th1 differentiation.

Alternative splice variants have been reported especially in the MCF7 cell line. The TWAJ Human/Mouse Gata-3 antibody will recognize both forms (50 and 45 kDa) of the protein.

Staining with the TWAJ Human/Mouse Gata-3 antibody requires the use of the Foxp3/Transcription Factor Staining Buffer Set.(Product # 00-5523-00)

Crossreactivity in rhesus monkeys has been published.

Applications Reported: This TWAJ antibody has been reported for use in intracellular staining followed by flow cytometric analysis.

Applications Tested: This TWAJ antibody has been pre-titrated and tested by intracellular staining and flow cytometric analysis of mouse thymocytes using the Foxp3/Transcription Factor Staining Buffer Set (Product # 00-5523) and protocol. Please see Best Protocols Section (Staining Intracellular Antigens for Flow Cytometry) for staining protocol (refer to Protocol B: One-step protocol for intracellular (nuclear) proteins). This can be used at 5 µL (0.06 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test.

Excitation: 488-561 nm; Emission: 578 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser.

Filtration: 0.2 µm post-manufacturing filtered.

Target Information

The genes for all 4 subunits of the T-cell antigen receptor (alpha, beta, gamma and delta) are controlled by distinct enhancers and their enhancer-binding proteins. Marine and Winoto (1991) identified a common TCR regulatory element by demonstrating binding of the enhancer-binding protein GATA3 to the enhancer elements of all 4 TCR genes. GATA3 had been shown in the chicken to be an enhancer-binding protein containing a zinc finger domain. GATA3 mRNA was demonstrated by Northern blot analysis in T cells but not in B cells or macrophages. GATA3 is abundantly expressed in the T-lymphocyte lineage and is thought to participate in T-cell receptor gene activation through binding to enhancers. Labastie et al. (1994) cloned the human gene and the 5-prime end of the mouse gene. The human gene comprises 6 exons distributed over 17 kb of DNA. Its 2 zinc fingers are encoded by 2 separate exons highly conserved with those of GATA1, but no other structural homologies between the 2 genes could be found.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Product References

RIPK3 controls MAIT cell accumulation during development but not during infection.

Cell death & disease

Patton T,Zhao Z,Lim XY,Eddy E,Wang H,Nelson AG,Ennis B,Eckle SBG,Souter MNT,Pediongco TJ,Koay HF,Zhang JG,Djajawi TM,Louis C,Lalaoui N,Jacquelot N,Lew AM,Pellicci DG,McCluskey J,Zhan Y,Chen Z,Lawlor KE,Corbett AJ

12-9966-42 was used in Flow cytometry/Cell sorting to suggest that, distinct from conventional T cells, the accumulation of MAIT cells is restrained by RIPK3 signalling, likely prior to thymic egress, in a manner independent of canonical apoptotic and necroptotic cell death pathways.

Sat Feb 11 00:00:00 EST 2023

The activity of the aryl hydrocarbon receptor in T cells tunes the gut microenvironment to sustain autoimmunity and neuroinflammation.

PLoS biology

Merchak AR,Cahill HJ,Brown LC,Brown RM,Rivet-Noor C,Beiter RM,Slogar ER,Olgun DG,Gaultier A

12-9966-42 was used in Flow cytometry/Cell sorting to demonstrate a newly emerging role for AHR in mediating the interdependence between T lymphocytes and the microbiota, while simultaneously identifying new potential molecular targets for the treatment of MS and other autoimmune diseases.

Wed Feb 01 00:00:00 EST 2023

The transcription factor HIF-1α mediates plasticity of NKp46+ innate lymphoid cells in the gut.

The Journal of experimental medicine

Krzywinska E,Sobecki M,Nagarajan S,Zacharjasz J,Tambuwala MM,Pelletier A,Cummins E,Gotthardt D,Fandrey J,Kerdiles YM,Peyssonnaux C,Taylor CT,Sexl V,Stockmann C

12-9966-42 was used in Flow cytometry/Cell sorting to suggest that HIF-1α shapes the ILC phenotype in the gut.

Mon Feb 07 00:00:00 EST 2022

Intestinal fibroblastic reticular cell niches control innate lymphoid cell homeostasis and function.

Nature communications

Cheng HW,Mörbe U,Lütge M,Engetschwiler C,Onder L,Novkovic M,Gil-Cruz C,Perez-Shibayama C,Hehlgans T,Scandella E,Ludewig B

12-9966-42 was used in flow cytometry to indicate critical fibroblastic niches within the intestinal lamina propria that control ILC homeostasis and functionality and thereby secure protective gut immunity.

Tue Apr 19 00:00:00 EDT 2022

Candida tropicalis Infection Modulates the Gut Microbiome and Confers Enhanced Susceptibility to Colitis in Mice.

Cellular and molecular gastroenterology and hepatology

Di Martino L,De Salvo C,Buela KA,Hager C,Ghannoum M,Osme A,Buttò L,Bamias G,Pizarro TT,Cominelli F

12-9966-42 was used in Flow Cytometry to determine the effects and mechanisms of action of Candida tropicalis on intestinal inflammation and injury in mouse models.

Mon Apr 04 00:00:00 EDT 2022

Eosinophils, but Not Type 2 Innate Lymphoid Cells, Are the Predominant Source of Interleukin 4 during the Innate Phase of Leishmania major Infection.

Pathogens (Basel, Switzerland)

Sasse C,Barinberg D,Obermeyer S,Debus A,Schleicher U,Bogdan C

12-9966-42 was used in Flow cytometry/Cell sorting to investigate if eosinophils, but not ILC2, are a major innate source of IL-4 at the skin site of L. major infection.

Mon Jul 25 00:00:00 EDT 2022

Guidelines for the use of flow cytometry and cell sorting in immunological studies (third edition).

European journal of immunology

Cossarizza A,Chang HD,Radbruch A,Abrignani S,Addo R,Akdis M,Andrä I,Andreata F,Annunziato F,Arranz E,Bacher P,Bari S,Barnaba V,Barros-Martins J,Baumjohann D,Beccaria CG,Bernardo D,Boardman DA,Borger J,Böttcher C,Brockmann L,Burns M,Busch DH,Cameron G,Cammarata I,Cassotta A,Chang Y,Chirdo FG,Christakou E,Čičin-Šain L,Cook L,Corbett AJ,Cornelis R,Cosmi L,Davey MS,De Biasi S,De Simone G,Del Zotto G,Delacher M,Di Rosa F,Di Santo J,Diefenbach A,Dong J,Dörner T,Dress RJ,Dutertre CA,Eckle SBG,Eede P,Evrard M,Falk CS,Feuerer M,Fillatreau S,Fiz-Lopez A,Follo M,Foulds GA,Fröbel J,Gagliani N,Galletti G,Gangaev A,Garbi N,Garrote JA,Geginat J,Gherardin NA,Gibellini L,Ginhoux F,Godfrey DI,Gruarin P,Haftmann C,Hansmann L,Harpur CM,Hayday AC,Heine G,Hernández DC,Herrmann M,Hoelsken O,Huang Q,Huber S,Huber JE,Huehn J,Hundemer M,Hwang WYK,Iannacone M,Ivison SM,Jäck HM,Jani PK,Keller B,Kessler N,Ketelaars S,Knop L,Knopf J,Koay HF,Kobow K,Kriegsmann K,Kristyanto H,Krueger A,Kuehne JF,Kunze-Schumacher H,Kvistborg P,Kwok I,Latorre D,Lenz D,Levings MK,Lino AC,Liotta F,Long HM,Lugli E,MacDonald KN,Maggi L,Maini MK,Mair F,Manta C,Manz RA,Mashreghi MF,Mazzoni A,McCluskey J,Mei HE,Melchers F,Melzer S,Mielenz D,Monin L,Moretta L,Multhoff G,Muñoz LE,Muñoz-Ruiz M,Muscate F,Natalini A,Neumann K,Ng LG,Niedobitek A,Niemz J,Almeida LN,Notarbartolo S,Ostendorf L,Pallett LJ,Patel AA,Percin GI,Peruzzi G,Pinti M,Pockley AG,Pracht K,Prinz I,Pujol-Autonell I,Pulvirenti N,Quatrini L,Quinn KM,Radbruch H,Rhys H,Rodrigo MB,Romagnani C,Saggau C,Sakaguchi S,Sallusto F,Sanderink L,Sandrock I,Schauer C,Scheffold A,Scherer HU,Schiemann M,Schildberg FA,Schober K,Schoen J,Schuh W,Schüler T,Schulz AR,Schulz S,Schulze J,Simonetti S,Singh J,Sitnik KM,Stark R,Starossom S,Stehle C,Szelinski F,Tan L,Tarnok A,Tornack J,Tree TIM,van Beek JJP,van de Veen W,van Gisbergen K,Vasco C,Verheyden NA,von Borstel A,Ward-Hartstonge KA,Warnatz K,Waskow C,Wiedemann A,Wilharm A,Wing J,Wirz O,Wittner J,Yang JHM,Yang J

12-9966-42 was used in Flow cytometry/Cell sorting to provide the key aspects to consider when performing flow cytometry experiments.

Wed Dec 01 00:00:00 EST 2021

Lymph node-resident dendritic cells drive T_H2 cell development involving MARCH1.

Science immunology

Castellanos CA,Ren X,Gonzalez SL,Li HK,Schroeder AW,Liang HE,Laidlaw BJ,Hu D,Mak ACY,Eng C,Rodríguez-Santana JR,LeNoir M,Yan Q,Celedón JC,Burchard EG,Zamvil SS,Ishido S,Locksley RM,Cyster JG,Huang X,Shin JS

12-9966-42 was used in Flow cytometry/Cell sorting to suggest that TH2 cell development depends on ubiquitin-mediated clearance of antigen-presenting and costimulatory molecules by LN-resident DCs and consequent control of TCR signaling.

Fri Oct 15 00:00:00 EDT 2021

Regulatory T-cells inhibit microglia-induced pain hypersensitivity in female mice.

eLife

Kuhn JA,Vainchtein ID,Braz J,Hamel K,Bernstein M,Craik V,Dahlgren MW,Ortiz-Carpena J,Molofsky AB,Molofsky AV,Basbaum AI

12-9966-42 was used in Flow Cytometry to conclude that sexual dimorphism in the contribution of microglia to pain results from Treg-mediated suppression of microglial activation and pain hypersensitivity in female mice.

Fri Oct 15 00:00:00 EDT 2021

Sex-specific adipose tissue imprinting of regulatory T cells.

Nature

Vasanthakumar A,Chisanga D,Blume J,Gloury R,Britt K,Henstridge DC,Zhan Y,Torres SV,Liene S,Collins N,Cao E,Sidwell T,Li C,Spallanzani RG,Liao Y,Beavis PA,Gebhardt T,Trevaskis N,Nutt SL,Zajac JD,Davey RA,Febbraio MA,Mathis D,Shi W,Kallies A

12-9966-42 was used in Flow Cytometry to find that sex-specific differences in Treg cells from visceral adipose tissue are determined by the tissue niche in a sex-hormone-dependent manner to limit adipose tissue inflammation.

Sun Mar 01 00:00:00 EST 2020

Insulin-Like Growth Factors Are Key Regulators of T Helper 17 Regulatory T Cell Balance in Autoimmunity.

Immunity

DiToro D,Harbour SN,Bando JK,Benavides G,Witte S,Laufer VA,Moseley C,Singer JR,Frey B,Turner H,Bruning J,Darley-Usmar V,Gao M,Conover C,Hatton RD,Frank S,Colonna M,Weaver CT

12-9966 was used in Flow cytometry/Cell sorting to show that, among effector T cell subsets, Th17 and Treg cells selectively expressed multiple components of the IGF system.

Tue Apr 14 00:00:00 EDT 2020

Dietary Glucose Consumption Promotes RALDH Activity in Small Intestinal CD103⁺CD11b⁺ Dendritic Cells.

Frontiers in immunology

Ko HJ,Hong SW,Verma R,Jung J,Lee M,Kim N,Kim D,Surh CD,Kim KS,Rudra D,Im SH

12-9966 was used in Flow cytometry/Cell sorting to reveal previously underappreciated role of dietary glucose concentration in establishing regulatory properties in intestinal DCs, thereby extending a potential therapeutic module against intestinal inflammation.

Mon Apr 19 00:00:00 EDT 2021

Type 2 Innate Lymphoid Cells Accumulate in the Brain After Hypoxia-Ischemia but Do Not Contribute to the Development of Preterm Brain Injury.

Frontiers in cellular neuroscience

Zelco A,Rocha-Ferreira E,Nazmi A,Ardalan M,Chumak T,Nilsson G,Hagberg H,Mallard C,Wang X

12-9966 was used in Flow cytometry/Cell sorting to investigate if innate lymphoid cells, especially ILC2s, play a role in preterm brain injury.

Sat Apr 16 00:00:00 EDT 2022

Attenuation of TCR-induced transcription by Bach2 controls regulatory T cell differentiation and homeostasis.

Nature communications

Sidwell T,Liao Y,Garnham AL,Vasanthakumar A,Gloury R,Blume J,Teh PP,Chisanga D,Thelemann C,de Labastida Rivera F,Engwerda CR,Corcoran L,Kometani K,Kurosaki T,Smyth GK,Shi W,Kallies A

12-9966 was used in Flow cytometry/Cell sorting to show homeostasis of thymically-derived and peripherally-derived Treg cells is maintained through the balancing of TCR signaling induced transcriptional activity by Bach2.

Tue Jan 14 00:00:00 EST 2020

Mitochondrial Oxidative Phosphorylation Regulates the Fate Decision between Pathogenic Th17 and Regulatory T Cells.

Cell reports

Shin B,Benavides GA,Geng J,Koralov SB,Hu H,Darley-Usmar VM,Harrington LE

12-9966 was used in Flow cytometry/Cell sorting to study the role of mitochondrial oxidative phosphorylation in pathogenic Th17 differentiation.

Tue Feb 11 00:00:00 EST 2020

Compartmentalized gut lymph node drainage dictates adaptive immune responses.

Nature

Esterházy D,Canesso MCC,Mesin L,Muller PA,de Castro TBR,Lockhart A,ElJalby M,Faria AMC,Mucida D

12-9966-42 was used in Flow Cytometry to show drainage of gut-draining lymph nodes can resolve conflicting tolerogenic and inflammatory intestinal responses by encouraging antigen targeting to specific gut segments.

Wed May 01 00:00:00 EDT 2019

Tissue-Resident Group 2 Innate Lymphoid Cells Differentiate by Layered Ontogeny and In Situ Perinatal Priming.

Immunity

Schneider C,Lee J,Koga S,Ricardo-Gonzalez RR,Nussbaum JC,Smith LK,Villeda SA,Liang HE,Locksley RM

12-9966 was used in Flow cytometry/Cell sorting to show that ILC2 appeared in multiple organs during lat gestation like tissue macrophages, but, unlike the latter, a majority of peripheral ILC2 pools were generated de novo during the postnatal window.

Tue Jun 18 00:00:00 EDT 2019

A Mutation in the Transcription Factor Foxp3 Drives T Helper 2 Effector Function in Regulatory T Cells.

Immunity

Van Gool F,Nguyen MLT,Mumbach MR,Satpathy AT,Rosenthal WL,Giacometti S,Le DT,Liu W,Brusko TM,Anderson MS,Rudensky AY,Marson A,Chang HY,Bluestone JA

12-9966 was used in Flow cytometry/Cell sorting to identify an IPEX (immune dysregulation polyendocrinopathy enteropathy X-linked) syndrome patient with a FOXP3 mutation in the domain swap interface of the protein.

Tue Feb 19 00:00:00 EST 2019

A chemical biology toolbox to study protein methyltransferases and epigenetic signaling.

Nature communications

Scheer S,Ackloo S,Medina TS,Schapira M,Li F,Ward JA,Lewis AM,Northrop JP,Richardson PL,Kaniskan HÜ,Shen Y,Liu J,Smil D,McLeod D,Zepeda-Velazquez CA,Luo M,Jin J,Barsyte-Lovejoy D,Huber KVM,De Carvalho DD,Vedadi M,Zaph C,Brown PJ,Arrowsmith CH

12-9966 was used in Flow cytometry/Cell sorting to present a collection of chemical probes and associated reagents and data to elucidate the function of human and murine protein methyltransferases in cellular studies.

Thu Jan 03 00:00:00 EST 2019

Ets1 suppresses atopic dermatitis by suppressing pathogenic T cell responses.

JCI insight

Lee CG,Kwon HK,Kang H,Kim Y,Nam JH,Won YH,Park S,Kim T,Kang K,Rudra D,Jun CD,Park ZY,Im SH

12-9966 was used in Flow cytometry/Cell sorting to reveal a protective role of Ets1 in restricting pathogenic Th cell responses and suggest a potential therapeutic target for atopic dermatitis (AD) treatment.

Thu Mar 07 00:00:00 EST 2019

Distinct immunocyte-promoting and adipocyte-generating stromal components coordinate adipose tissue immune and metabolic tenors.

Science immunology

Spallanzani RG,Zemmour D,Xiao T,Jayewickreme T,Li C,Bryce PJ,Benoist C,Mathis D

12-9966 was used in Flow cytometry/Cell sorting to identify and further characterize the cellular sources of IL-33 within visceral adipose tissue (VAT) and to determine how these cells correlate with local Tregs in diverse physiologic and pathologic states.

Fri May 03 00:00:00 EDT 2019

TNFR2 Signaling Enhances ILC2 Survival, Function, and Induction of Airway Hyperreactivity.

Cell reports

Hurrell BP,Galle-Treger L,Jahani PS,Howard E,Helou DG,Banie H,Soroosh P,Akbari O

12-9966 was used in Flow cytometry/Cell sorting to investigate the role of TNF/TNFR2 axis in pulmonary ILC2s for possible use in treating ILC2-dependent asthma, in murine and human subjects.

Tue Dec 24 00:00:00 EST 2019

Female and male mouse lung group 2 innate lymphoid cells differ in gene expression profiles and cytokine production.

PloS one

Mathä L,Shim H,Steer CA,Yin YH,Martinez-Gonzalez I,Takei F

12-9966-42 was used in Flow Cytometry to suggest that female ILC2s are more readily activated than male ILC2s due to their gene expression at the naïve state, which is potentially influenced by the lung environment.

Mon Dec 16 00:00:00 EST 2019

TCR Transgenic Mice Reveal Stepwise, Multi-site Acquisition of the Distinctive Fat-Treg Phenotype.

Cell

Li C,DiSpirito JR,Zemmour D,Spallanzani RG,Kuswanto W,Benoist C,Mathis D

12-9966-42 was used in Flow Cytometry to establish a role for T cell receptor specificity, uncovered an unexpected function for the primordial Treg transcription-factor, Foxp3, evidenced a cell-intrinsic role for interleukin-33 receptor, and ordered these dependencies within a coherent scenario.

Thu Jul 12 00:00:00 EDT 2018

Proteomic Analyses of Human Regulatory T Cells Reveal Adaptations in Signaling Pathways that Protect Cellular Identity.

Immunity

Cuadrado E,van den Biggelaar M,de Kivit S,Chen YY,Slot M,Doubal I,Meijer A,van Lier RAW,Borst J,Amsen D

12-9966 was used in Flow cytometry/Cell sorting to suggest that adaptation in signaling pathways protect Treg cell identity and present a resource for further research into Treg cell biology.

Tue May 15 00:00:00 EDT 2018

E3 Ligase VHL Promotes Group 2 Innate Lymphoid Cell Maturation and Function via Glycolysis Inhibition and Induction of Interleukin-33 Receptor.

Immunity

Li Q,Li D,Zhang X,Wan Q,Zhang W,Zheng M,Zou L,Elly C,Lee JH,Liu YC

12-9966 was used in Flow Cytometry to indicate that the VHL-HIF-glycolysis axis is essential for the late-stage maturation and function of ILC2s via targeting IL-33-ST2 pathway.

Tue Feb 20 00:00:00 EST 2018

Autoimmune Th17 Cells Induced Synovial Stromal and Innate Lymphoid Cell Secretion of the Cytokine GM-CSF to Initiate and Augment Autoimmune Arthritis.

Immunity

Hirota K,Hashimoto M,Ito Y,Matsuura M,Ito H,Tanaka M,Watanabe H,Kondoh G,Tanaka A,Yasuda K,Kopf M,Potocnik AJ,Stockinger B,Sakaguchi N,Sakaguchi S

12-9966 was used in Flow cytometry/Cell sorting to study the signalling pathway responsible for mediating the stimulation of fibroblast-like synoviocytes by arthritogenic Th17 cells.

Tue Jun 19 00:00:00 EDT 2018

CD4⁺ T Cell Fate Decisions Are Stochastic, Precede Cell Division, Depend on GITR Co-Stimulation, and Are Associated With Uropodium Development.

Frontiers in immunology

Cobbold SP,Adams E,Howie D,Waldmann H

12-9966 was used in Flow cytometry/Cell sorting to develop an in vitro model of T cell differentiation suited to testing how manipulation of components of the microenvironment might be therapeutically exploited.

Mon Jan 31 00:00:00 EST 2022

β-Arrestin-2-Dependent Signaling Promotes CCR4-mediated Chemotaxis of Murine T-Helper Type 2 Cells.

American journal of respiratory cell and molecular biology

Lin R,Choi YH,Zidar DA,Walker JKL

12-9966 was used in Flow cytometry/Cell sorting to point to a proinflammatory role for β-arr2-dependent signaling and support β-arr2 as a novel therapeutic target in asthma.

Fri Jun 01 00:00:00 EDT 2018

Effects of Anti-Integrin Treatment With Vedolizumab on Immune Pathways and Cytokines in Inflammatory Bowel Diseases.

Frontiers in immunology

Rath T,Billmeier U,Ferrazzi F,Vieth M,Ekici A,Neurath MF,Atreya R

12-9966 was used in Flow cytometry/Cell sorting to identify genetic, cellular, and immunological mechanisms that define response and failure to vedolizumab treatment.

Mon Sep 09 00:00:00 EDT 2019

Regulatory T cells are required for normal and activin-promoted wound repair in mice.

European journal of immunology

Haertel E,Joshi N,Hiebert P,Kopf M,Werner S

12-9966-42 was used in Flow cytometry/Cell sorting to demonstrate that Treg depletion in wounds facilitates the expansion of an αβ T-cell population with features of Th1 and Th2 cells, and suggest that concomitant changes in the cytokine milieu disturb the healing process.

Fri Jun 01 00:00:00 EDT 2018

The Combination Therapy of Dietary Galacto-Oligosaccharides With Budesonide Reduces Pulmonary Th2 Driving Mediators and Mast Cell Degranulation in a Murine Model of House Dust Mite Induced Asthma.

Frontiers in immunology

Verheijden KAT,Braber S,Leusink-Muis T,Jeurink PV,Thijssen S,Kraneveld AD,Garssen J,Folkerts G,Willemsen LEM

12-9966 was used in Flow cytometry/Cell sorting to compare the efficacy of budesonide or different doses of galacto-oligosaccharides alone or with a combination therapy of budesonide and galacto-oligosaccharides on house dust mite-allergic responses in mice.

Mon Sep 30 00:00:00 EDT 2019

ILC2s regulate adaptive Th2 cell functions via PD-L1 checkpoint control.

The Journal of experimental medicine

Schwartz C,Khan AR,Floudas A,Saunders SP,Hams E,Rodewald HR,McKenzie ANJ,Fallon PG

12-9966 was used in Flow cytometry/Cell sorting to study how group 2 innate lymphoid cells regulate adaptive Th2 cell functions.

Mon Sep 04 00:00:00 EDT 2017

Primary Heligmosomoides polygyrus bakeri infection induces myeloid-derived suppressor cells that suppress CD4⁺ Th2 responses and promote chronic infection.

Mucosal immunology

Valanparambil RM,Tam M,Jardim A,Geary TG,Stevenson MM

12-9966 was used in Flow cytometry/Cell sorting to study the exact identity and role of the F4/80-CD11b+Gr+ cells accumulating around encysting larvae in intestinal tissue during primary Heligmosomoides polygyrus bakeri infection in mice.

Sun Jan 01 00:00:00 EST 2017

IRF-8 regulates expansion of myeloid-derived suppressor cells and Foxp3+ regulatory T cells and modulates Th2 immune responses to gastrointestinal nematode infection.

PLoS pathogens

Valanparambil RM,Tam M,Gros PP,Auger JP,Segura M,Gros P,Jardim A,Geary TG,Ozato K,Stevenson MM

12-9966 was used in Flow cytometry/Cell sorting to study the role of interferon regulatory factor-8 on Th2 immunity to Helimosomoides polygyrus bakeri infection.

Sun Oct 01 00:00:00 EDT 2017

Composition of the Schistosoma mansoni worm secretome: Identification of immune modulatory Cyclophilin A.

PLoS neglected tropical diseases

Floudas A,Cluxton CD,Fahel J,Khan AR,Saunders SP,Amu S,Alcami A,Fallon PG

12-9966 was used in Flow cytometry/Cell sorting to demonstrate the unique protein composition of the S. mansoni male worm secretome and immunomodulatory activity of S. mansoni Cyclophilin A.

Sun Oct 01 00:00:00 EDT 2017

Intratumoral modulation of the inducible co-stimulator ICOS by recombinant oncolytic virus promotes systemic anti-tumour immunity.

Nature communications

Zamarin D,Holmgaard RB,Ricca J,Plitt T,Palese P,Sharma P,Merghoub T,Wolchok JD,Allison JP

12-9966 was used in Flow cytometry/Cell sorting to highlight that intratumoral immunomodulation with an oncolytic virus expressing a rationally selected ligand can be an effective strategy to drive systemic efficacy of immune checkpoint blockade.

Mon Feb 13 00:00:00 EST 2017

Treatment with cyclophosphamide supported by various dendritic cell-based vaccines induces diversification in CD4⁺ T cell response against MC38 colon carcinoma.

International journal of oncology

Wojas-Turek J,Szczygieł A,Kicielińska J,Rossowska J,Piasecki E,Pajtasz-Piasecka E

12-9966 was used in Flow cytometry/Cell sorting to show that an application of cyclophosphamide supported by dendritic cell based vaccines affected differentiation of the activity of CD4+ T cell subpopulations accompanied by an alteration in CD8+ cell number.

Mon Feb 01 00:00:00 EST 2016

Characterization and Quantification of Innate Lymphoid Cell Subsets in Human Lung.

PloS one

De Grove KC,Provoost S,Verhamme FM,Bracke KR,Joos GF,Maes T,Brusselle GG

12-9966 was used in Flow cytometry/Cell sorting to study innate lymphoid cells in the human lung in healthy patients and those with chronic pulmonary disease.

Fri Jul 08 00:00:00 EDT 2016

Group 2 innate lymphoid cells promote beiging of white adipose tissue and limit obesity.

Nature

Brestoff JR,Kim BS,Saenz SA,Stine RR,Monticelli LA,Sonnenberg GF,Thome JJ,Farber DL,Lutfy K,Seale P,Artis D

12-9966 was used in Flow cytometry/Cell sorting to study how ILC2s can regulate adipose function and metabolic homeostasis.

Thu Mar 12 00:00:00 EDT 2015

The immunosuppressive enzyme IL4I1 promotes FoxP3(+) regulatory T lymphocyte differentiation.

European journal of immunology

Cousin C,Aubatin A,Le Gouvello S,Apetoh L,Castellano F,Molinier-Frenkel V

12-9966 was used in Flow cytometry/Cell sorting to study the effect of IL4I1 on naive CD4+ T cell differentiation.

Mon Jun 01 00:00:00 EDT 2015

Expression of GM-CSF in T Cells Is Increased in Multiple Sclerosis and Suppressed by IFN-β Therapy.

Journal of immunology (Baltimore, Md. : 1950)

Rasouli J,Ciric B,Imitola J,Gonnella P,Hwang D,Mahajan K,Mari ER,Safavi F,Leist TP,Zhang GX,Rostami A

12-9966 was used in Flow cytometry/Cell sorting to characterise GM-CSF production by T cells of MS patients and to determine the effect of IFN-β therapy on its production.

Mon Jun 01 00:00:00 EDT 2015

Triclosan Induces Thymic Stromal Lymphopoietin in Skin Promoting Th2 Allergic Responses.

Toxicological sciences : an official journal of the Society of Toxicology

Marshall NB,Lukomska E,Long CM,Kashon ML,Sharpnack DD,Nayak AP,Anderson KL,Jean Meade B,Anderson SE

12-9966 was used in Flow cytometry/Cell sorting to examine the effects of dermal triclosan exposure on the skin and lymph nodes of mice and in a human skin model to identify mechanisms for augmenting allergic responses.

Tue Sep 01 00:00:00 EDT 2015

Mesenchymal Stem Cells Reduce Murine Atherosclerosis Development.

Scientific reports

Frodermann V,van Duijn J,van Pel M,van Santbrink PJ,Bot I,Kuiper J,de Jager SC

12-9966 was used in Flow cytometry/Cell sorting to study how MSC treatment affects not only inflammatory responses but also significantly reduces dyslipidaemia in mice.

Thu Oct 22 00:00:00 EDT 2015

Hyperreactive onchocerciasis is characterized by a combination of Th17-Th2 immune responses and reduced regulatory T cells.

PLoS neglected tropical diseases

Katawa G,Layland LE,Debrah AY,von Horn C,Batsa L,Kwarteng A,Arriens S,W Taylor D,Specht S,Hoerauf A,Adjobimey T

12-9966 was used in Flow cytometry/Cell sorting to provide initial evidence that elevated frequencies of Th17 and Th2 cells form part of the immune network instigating the development of severe onchocerciasis.

Thu Jan 01 00:00:00 EST 2015

Microbial Ligand Costimulation Drives Neutrophilic Steroid-Refractory Asthma.

PloS one

Hadebe S,Kirstein F,Fierens K,Chen K,Drummond RA,Vautier S,Sajaniemi S,Murray G,Williams DL,Redelinghuys P,Reinhart TA,Fallert Junecko BA,Kolls JK,Lambrecht BN,Brombacher F,Brown GD

12-9966 was used in Flow cytometry/Cell sorting to investigate the effects of LPS and β-glucan on the pathogenesis of allergic and non-allergic respiratory responses to house dust mite allergen.

Tue May 17 00:00:00 EDT 2016

Activated innate lymphoid cells are associated with a reduced susceptibility to graft-versus-host disease.

Blood

Munneke JM,Björklund AT,Mjösberg JM,Garming-Legert K,Bernink JH,Blom B,Huisman C,van Oers MH,Spits H,Malmberg KJ,Hazenberg MD

12-9966 was used in Flow cytometry/Cell sorting to suggest that innate lymphoid cell recovery and treatment-related tissue damage are interrelated and affect the development of GVHD.

Thu Jul 31 00:00:00 EDT 2014

MicroRNA-155 is essential for T(H)2-mediated allergen-induced eosinophilic inflammation in the lung.

The Journal of allergy and clinical immunology

Malmhäll C,Alawieh S,Lu Y,Sjöstrand M,Bossios A,Eldh M,Rådinger M

12-9966 was used in Flow cytometry/Cell sorting to investigate the contribution of microRNA-155 to TH2-mediated allergen-induced airway inflammation in a mouse model.

Thu May 01 00:00:00 EDT 2014

T cell-derived IL-4/IL-13 protects mice against fatal Schistosoma mansoni infection independently of basophils.

Journal of immunology (Baltimore, Md. : 1950)

Schwartz C,Oeser K,Prazeres da Costa C,Layland LE,Voehringer D

12-9966 was used in Flow cytometry/Cell sorting to determine the contribution of cytokines and basophils for protective immunity against S. mansoni egg-induced pathology.

Wed Oct 01 00:00:00 EDT 2014

Phenotypic Identification of Spinal Cord-Infiltrating CD4⁺ T Lymphocytes in a Murine Model of Neuropathic Pain.

Journal of pain & relief

Draleau K,Maddula S,Slaiby A,Nutile-McMenemy N,De Leo J,Cao L

12-9966 was used in Flow cytometry/Cell sorting to investigate the phenotype of spinal cord-infiltrating CD4+ T lymphocytes involved in the maintenance of neuropathic pain in a murine model.

Sun Jun 01 00:00:00 EDT 2014

The transcription factor GATA3 is essential for the function of human type 2 innate lymphoid cells.

Immunity

Mjösberg J,Bernink J,Golebski K,Karrich JJ,Peters CP,Blom B,te Velde AA,Fokkens WJ,van Drunen CM,Spits H

12-9966 was used in Flow cytometry/Cell sorting to investigate the role of GATA3 in type 2 innate lymphoid cells.

Fri Oct 19 00:00:00 EDT 2012

STAT6 regulates natural helper cell proliferation during lung inflammation initiated by Alternaria.

American journal of physiology. Lung cellular and molecular physiology

Doherty TA,Khorram N,Chang JE,Kim HK,Rosenthal P,Croft M,Broide DH

12-9966 was used in Flow cytometry/Cell sorting to examine whether Alternaria, an aeroallergen specifically associated with severe asthma, induces a unique innate immune response compared with other allergens in mice.

Mon Oct 01 00:00:00 EDT 2012

CD69 regulates type I IFN-induced tolerogenic signals to mucosal CD4 T cells that attenuate their colitogenic potential.

Journal of immunology (Baltimore, Md. : 1950)

Radulovic K,Manta C,Rossini V,Holzmann K,Kestler HA,Wegenka UM,Nakayama T,Niess JH

12-9966 was used in Flow cytometry/Cell sorting to demonstrate that CD69 knockout can modulate mucosal CD4 T cells and increase colitis levels in a mouse transfer model of the disease.

Wed Feb 15 00:00:00 EST 2012

IL-1 family members IL-18 and IL-33 upregulate the inflammatory potential of differentiated human Th1 and Th2 cultures.

Journal of immunology (Baltimore, Md. : 1950)

Blom L,Poulsen LK

12-9966 was used in Flow cytometry/Cell sorting to demonstrate that IL-1 family "alarmins" IL-18 and IL-33, amplify Th1- and Th2-associated cytokines, and block IL-10 production in Th1 cultures.

Thu Nov 01 00:00:00 EDT 2012

Th-1 lymphocytes induce dendritic cell tumor killing activity by an IFN-γ-dependent mechanism.

Journal of immunology (Baltimore, Md. : 1950)

LaCasse CJ,Janikashvili N,Larmonier CB,Alizadeh D,Hanke N,Kartchner J,Situ E,Centuori S,Har-Noy M,Bonnotte B,Katsanis E,Larmonier N

12-9966 was used in Flow cytometry/Cell sorting to investigate the regulation of murine dendritic cell cytotoxic functions by T lymphocytes.

Thu Dec 15 00:00:00 EST 2011

Requirement of GATA-binding protein 3 for II13 gene expression in IL-18-stimulated Th1 cells.

International immunology

Nakahira M,Nakanishi K

12-9966 was used in Flow cytometry/Cell sorting to elucidate the molecular mechanism by which gene expression pattern is altered in T(h)1 cells in response to IL-18, showing that Gata3 is required for this altered gene expression.

Thu Dec 01 00:00:00 EST 2011

IL-33 induces IL-9 production in human CD4+ T cells and basophils.

PloS one

Blom L,Poulsen BC,Jensen BM,Hansen A,Poulsen LK

12-9966 was used in Flow cytometry/Cell sorting to indicate that barrier functioning cells and the cytokine IL-33 are important for the regulation of IL-9 production by immune cells in inflamed tissue.

Wed Nov 09 00:00:00 EST 2011

FOXP3+ regulatory T cells and T helper 1, T helper 2, and T helper 17 cells in human early pregnancy decidua.

Biology of reproduction

Mjösberg J,Berg G,Jenmalm MC,Ernerudh J

12-9966 was used in Flow cytometry/Cell sorting to study the chemokine receptor expression profiles in human pregnancy decidua to monitor levels of TH1, TH2, TH17 and Treg cells.

Thu Apr 01 00:00:00 EDT 2010

Bioinformatics

Protein Aliases: GATA binding factor-3; GATA binding protein-3; GATA-binding factor 3; GATA-binding protein 3; MGC2346; MGC5199; MGC5445; Trans-acting T-cell-specific transcription factor GATA-3; Transacting T-cell-specific transcription factor GATA-3

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Gene Aliases: EGK_19445; Gata-3; GATA3; HDR; HDRS; jal

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UniProt ID: (Human) P23771, (Mouse) P23772

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Entrez Gene ID: (Pig) 733631, (Rhesus monkey) 713840, (Human) 2625, (Mouse) 14462

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Function(s)

transcription regulatory region sequence-specific DNA binding RNA polymerase II regulatory region sequence-specific DNA binding RNA polymerase II core promoter sequence-specific DNA binding core promoter proximal region sequence-specific DNA binding core promoter sequence-specific DNA binding nucleic acid binding transcription factor activity transcriptional activator activity, RNA polymerase II core promoter proximal region sequence-specific binding transcriptional repressor activity, RNA polymerase II core promoter proximal region sequence-specific binding RNA polymerase II transcription factor binding enhancer sequence-specific DNA binding transcriptional activator activity, RNA polymerase II transcription regulatory region sequence-specific binding DNA binding chromatin binding transcription factor activity, sequence-specific DNA binding transcription coactivator activity interleukin-2 receptor binding protein binding transcription factor binding zinc ion binding transcription regulatory region DNA binding protein dimerization activity E-box binding HMG box domain binding sequence-specific DNA binding metal ion binding DNA-binding transcription factor

Process(es)

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Gata-3 Monoclonal Antibody (TWAJ), PE, eBioscience™

Gata-3 Antibody (12-9966-42) in Flow

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12-9966-42

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