Invitrogen

CD39 Monoclonal Antibody (24DMS1), eBioscience™

9 Published Figures

16 References

View all (78) CD39 antibodies

Datasheet

Protocols

Questions & Answers

Datasheet

Protocols

Questions & Answers

Antibody Testing Data (1)
Published Figures (9)

FIGURE: 1 / 10

CD39 Antibody (14-0391-82) in WB

Cell lysates prepared from mouse spleen (lane 1), thymocytes (lane 2), or bone marrow (lane 3) were resolved by SDS-PAGE prior to transfer onto a PVDF membrane. The membrane was probed with 2 µg/mL Anti-Mouse CD39 Purified and bands were detected using an HRP-conjugated anti-rat IgG antibody. Note: This antibody works only with non-reduced protein without boiling prior to gel loading.

CD39 Antibody in Western Blot, Flow Cytometry (WB, Flow)

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Product Details

14-0391-82

Applications

Tested Dilution

Publications

Western Blot (WB)

Assay-Dependent

View 1 publication 1 publication

Flow Cytometry (Flow)

0.06 µg/test

View 14 publications 14 publications

Miscellaneous PubMed (Misc)

View 1 publication 1 publication

Product Specifications

Species Reactivity

Mouse

Published species

Mouse

Host/Isotype

Rat / IgG2b, kappa

Class

Monoclonal

Type

Antibody

Clone

24DMS1

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.5 mg/mL

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.2

Contains

0.09% sodium azide

Storage conditions

4° C

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_1210501

Product Specific Information

Description: The 24DMS1 monoclonal antibody reacts with mouse CD39, also known as NTPDaseI. E-NTPDases are enzymes that convert nucleoside tri- and diphosphates (NTDPs) into nucleoside monophosphate (NMP), thereby removing toxic extracellular ATP and ADP. CD39 is the dominant member of this family in the immune system and is involved in suppression of inflammation and control of platelet activation. CD39 can impact expression of CD73, another E-NTPase. Together, these molecules influence inflammation responses. CD39 is expressed on B cells, Langerhans cells and most monocytes. In addition, CD39 is found on a subset of CD4+ T cells that are mostly CD25+FoxP3+ T reg cells. T reg cells from CD39-null mice showed impaired suppressive properties in vitro and in vivo.

Applications Reported: This 24DMS1 antibody has been reported for use in flow cytometric analysis, and immunoblotting (WB).

Applications Tested: This 24DMS1 antibody has been tested by flow cytometric or immunoblot analysis of mouse splenocytes. This can be used at less than or equal to 0.06 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

Purity: Greater than 90%, as determined by SDS-PAGE.

Aggregation: Less than 10%, as determined by HPLC.

Filtration: 0.2 µm post-manufacturing filtered.

Target Information

CD39 cell surface antigen is a 70-100kD molecule expressed on peripheral blood B cells, monocytes, T cell clones, and also weakly expressed on granulocytes. CD39 has intrinsic ecto-ATPase activity. Expression of CD39 is induced on T cells and increased on B cells as a late activation antigen.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Early innate and adaptive immune perturbations determine long-term severity of chronic virus and Mycobacterium tuberculosis coinfection.

Immunity

Xu W,Snell LM,Guo M,Boukhaled G,Macleod BL,Li M,Tullius MV,Guidos CJ,Tsao MS,Divangahi M,Horwitz MA,Liu J,Brooks DG

14-0391-82 was used in Miscellaneous to examine how chronic viral infections alter the pulmonary microenvironment to foster coinfection and worsen disease severity.

Tue Mar 09 00:00:00 EST 2021

Single-cell analysis defines a pancreatic fibroblast lineage that supports anti-tumor immunity.

Cancer cell

Hutton C,Heider F,Blanco-Gomez A,Banyard A,Kononov A,Zhang X,Karim S,Paulus-Hock V,Watt D,Steele N,Kemp S,Hogg EKJ,Kelly J,Jackstadt RF,Lopes F,Menotti M,Chisholm L,Lamarca A,Valle J,Sansom OJ,Springer C,Malliri A,Marais R,Pasca di Magliano M,Zelenay S,Morton JP,Jørgensen C

14-0391-82 was used in Flow cytometry/Cell sorting to reveal two functionally distinct pancreatic fibroblast lineages and highlight the importance of mesenchymal and immune cell interactions in restricting tumor growth.

Mon Sep 13 00:00:00 EDT 2021

Selective deletion of ENTPD1/CD39 in macrophages exacerbates biliary fibrosis in a mouse model of sclerosing cholangitis.

Purinergic signalling

Rothweiler S,Feldbrügge L,Jiang ZG,Csizmadia E,Longhi MS,Vaid K,Enjyoji K,Popov YV,Robson SC

Published figure using CD39 monoclonal antibody (Product # 14-0391-82) in Flow Cytometry

Sun Sep 01 00:00:00 EDT 2019

Toll-Like Receptor-Mediated Activation of CD39 Internalization in BMDCs Leads to Extracellular ATP Accumulation and Facilitates P2X7 Receptor Activation.

Frontiers in immunology

Zhao R,Qiao J,Zhang X,Zhao Y,Meng X,Sun D,Peng X

Published figure using CD39 monoclonal antibody (Product # 14-0391-82) in Flow Cytometry

Thu Oct 15 00:00:00 EDT 2020

Toll-Like Receptor-Mediated Activation of CD39 Internalization in BMDCs Leads to Extracellular ATP Accumulation and Facilitates P2X7 Receptor Activation.

Frontiers in immunology

Zhao R,Qiao J,Zhang X,Zhao Y,Meng X,Sun D,Peng X

Published figure using CD39 monoclonal antibody (Product # 14-0391-82) in Flow Cytometry

Thu Oct 15 00:00:00 EDT 2020

IL-27 targets Foxp3+ Tregs to mediate antiinflammatory functions during experimental allergic airway inflammation.

JCI insight

Nguyen QT,Jang E,Le HT,Kim S,Kim D,Dvorina N,Aronica MA,Baldwin WM,Asosingh K,Comhair S,Min B

14-0391 was used in Flow cytometry/Cell sorting to discover that Tregs during allergic airway inflammation are the primary target cells of IL-27 in order to mediate antinflammatory functions.

Thu Jan 24 00:00:00 EST 2019

CD150^high Bone Marrow Tregs Maintain Hematopoietic Stem Cell Quiescence and Immune Privilege via Adenosine.

Cell stem cell

Hirata Y,Furuhashi K,Ishii H,Li HW,Pinho S,Ding L,Robson SC,Frenette PS,Fujisaki J

Published figure using CD39 monoclonal antibody (Product # 14-0391-82) in Flow Cytometry

Thu Mar 01 00:00:00 EST 2018

Differentiation of human and murine induced pluripotent stem cells to microglia-like cells.

Nature neuroscience

Pandya H,Shen MJ,Ichikawa DM,Sedlock AB,Choi Y,Johnson KR,Kim G,Brown MA,Elkahloun AG,Maric D,Sweeney CL,Gossa S,Malech HL,McGavern DB,Park JK

Published figure using CD39 monoclonal antibody (Product # 14-0391-82) in Flow Cytometry

Mon May 01 00:00:00 EDT 2017

Membrane-organizing protein moesin controls Treg differentiation and antitumor immunity via TGF-β signaling.

The Journal of clinical investigation

Ansa-Addo EA,Zhang Y,Yang Y,Hussey GS,Howley BV,Salem M,Riesenberg B,Sun S,Rockey DC,Karvar S,Howe PH,Liu B,Li Z

14-0391 was used in Flow cytometry/Cell sorting to investigate whether moesin plays a role during the generation of TGF-β-induced Tregs, showing that it controls Treg differentiation and antitumor immunity.

Mon Apr 03 00:00:00 EDT 2017

CD73 regulates anti-inflammatory signaling between apoptotic cells and endotoxin-conditioned tissue macrophages.

Cell death and differentiation

Murphy PS,Wang J,Bhagwat SP,Munger JC,Janssen WJ,Wright TW,Elliott MR

14-0391 was used in Flow cytometry/Cell sorting to show that, in RAW264.7 cells, ectopic expression of both A2a and CD73 was required for TNF suppression by apoptotic cells.

Wed Mar 01 00:00:00 EST 2017

The BAFF receptor TACI controls IL-10 production by regulatory B cells and CLL B cells.

Leukemia

Saulep-Easton D,Vincent FB,Quah PS,Wei A,Ting SB,Croce CM,Tam C,Mackay F

14-0391 was used in Flow cytometry/Cell sorting to uncover a major targetable pathway important for the generation of regulatory B cells that is detrimental to immunity in chronic lymphocytic leukemia.

Fri Jan 01 00:00:00 EST 2016

CD39 Expression Identifies Terminally Exhausted CD8+ T Cells.

PLoS pathogens

Gupta PK,Godec J,Wolski D,Adland E,Yates K,Pauken KE,Cosgrove C,Ledderose C,Junger WG,Robson SC,Wherry EJ,Alter G,Goulder PJ,Klenerman P,Sharpe AH,Lauer GM,Haining WN

14-0391 was used in Flow cytometry/Cell sorting to compare the expression of CD39 by CD8+ T cells from healthy donors and subjects with chronic viral infections.

Thu Oct 01 00:00:00 EDT 2015

Interleukin-35 administration counteracts established murine type 1 diabetes--possible involvement of regulatory T cells.

Scientific reports

Singh K,Kadesjö E,Lindroos J,Hjort M,Lundberg M,Espes D,Carlsson PO,Sandler S,Thorvaldson L

14-0391 was used in Flow cytometry/Cell sorting to suggest that insufficient IL-35 levels play a pivotal role in the development of T1D and that treatment with IL-35 should be investigated in treatment of T1D and other autoimmune diseases.

Thu Jul 30 00:00:00 EDT 2015

Cutaneous immunosurveillance and regulation of inflammation by group 2 innate lymphoid cells.

Nature immunology

Roediger B,Kyle R,Yip KH,Sumaria N,Guy TV,Kim BS,Mitchell AJ,Tay SS,Jain R,Forbes-Blom E,Chen X,Tong PL,Bolton HA,Artis D,Paul WE,Fazekas de St Groth B,Grimbaldeston MA,Le Gros G,Weninger W

14-0391 was used in Flow cytometry/Cell sorting to report the identification in normal mouse dermis of an abundant, phenotypically unique group 2 innate lymphoid cell subset that depended on interleukin 7 and constitutively produced IL-13.

Sat Jun 01 00:00:00 EDT 2013

The transcription cofactor Hopx is required for regulatory T cell function in dendritic cell-mediated peripheral T cell unresponsiveness.

Nature immunology

Hawiger D,Wan YY,Eynon EE,Flavell RA

14-0391 was used in Flow cytometry/Cell sorting to study the role of the homeodomain-only protein (Hopx) in induced regulatory T cells (iT(regs)).

Fri Oct 01 00:00:00 EDT 2010

Self-regulation of inflammatory cell trafficking in mice by the leukocyte surface apyrase CD39.

The Journal of clinical investigation

Hyman MC,Petrovic-Djergovic D,Visovatti SH,Liao H,Yanamadala S,Bouïs D,Su EJ,Lawrence DA,Broekman MJ,Marcus AJ,Pinsky DJ

Published figure using CD39 monoclonal antibody (Product # 14-0391-82) in Flow Cytometry

Fri May 01 00:00:00 EDT 2009

Bioinformatics

Protein Aliases: ATP diphosphohydrolase; ATP-DPH; CD39; DKFZp686D194; DKFZp686I093; Ecto-apyrase; Ecto-ATP diphosphohydrolase 1; Ecto-ATPDase 1; Ectonucleoside triphosphate diphosphohydrolase 1; FLJ40921; FLJ40959; Lymphoid cell activation antigen; NTPDase 1; NTPDase1; Nucleoside triphosphate diphosphohydrolase 1

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Gene Aliases: 2610206B08Rik; AA408691; Cd39; Entpd1; NTPDase-1

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UniProt ID: (Mouse) P55772

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Entrez Gene ID: (Mouse) 12495

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Function(s)

hydrolase nucleotide phosphatase phosphatase metabolite interconversion enzyme

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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CD39 Monoclonal Antibody (24DMS1), eBioscience™

CD39 Antibody (14-0391-82) in WB

Product Details

14-0391-82

Western Blot (WB)

Flow Cytometry (Flow)

Miscellaneous PubMed (Misc)

Species Reactivity

Published species

Host/Isotype

Class

Type

Clone

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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