Invitrogen

CD39 Monoclonal Antibody (24DMS1), PE, eBioscience™

9 Published Figures

16 References

View all (78) CD39 antibodies

Datasheet

Protocols

Questions & Answers

Datasheet

Protocols

Questions & Answers

Antibody Testing Data (1)
Published Figures (9)

FIGURE: 1 / 10

CD39 Antibody (12-0391-82) in Flow

Staining of mouse splenocytes with 0.125 µg Anti-Mouse CD39 PE and Anti-Mouse CD4 FITC (Product # 11-0041-82), followed by fixation and permeabilization with the Foxp3 Staining Buffers (Product # 00-5523-00), and intracellular staining with Anti-Mouse Foxp3 Alexa Fluor® 647. The histogram (right) demonstrates staining of Anti-Mouse CD39 PE on CD4+Foxp3. Cells (purple histogram) and CD4+Foxp3+ cells (blue histogram) as gated in the dot plot (left).

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Product Details

12-0391-82

Applications

Tested Dilution

Publications

Flow Cytometry (Flow)

0.25 µg/test

View 16 publications 16 publications

Product Specifications

Species Reactivity

Mouse

Published species

Mouse

Host/Isotype

Rat / IgG2b, kappa

Recommended Isotype Control

Rat IgG2b kappa Isotype Control (eB149/10H5), PE, eBioscience™

Class

Monoclonal

Type

Antibody

Clone

24DMS1

Conjugate

PE PE PE

Excitation/Emission Max

565/576 nm View spectra

Form

Liquid

Concentration

0.2 mg/mL

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.2

Contains

0.09% sodium azide

Storage conditions

4° C, store in dark, DO NOT FREEZE!

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_1210740

Product Specific Information

Description: The 24DMS1 monoclonal antibody reacts with mouse CD39, also known as NTPDaseI. E-NTPDases are enzymes that convert nucleoside tri- and diphosphates (NTDPs) into nucleoside monophosphate (NMP), thereby removing toxic extracellular ATP and ADP. CD39 is the dominant member of this family in the immune system and is involved in suppression of inflammation and control of platelet activation. CD39 can impact expression of CD73, another E-NTPase. Together, these molecules influence inflammation responses. CD39 is expressed on B cells, Langerhans cells and most monocytes. In addition, CD39 is found on a subset of CD4+ T cells that are mostly CD25+FoxP3+ T reg cells. T reg cells from CD39-null mice showed impaired suppressive properties in vitro and in vivo.

Applications Reported: This 24DMS1 antibody has been reported for use in flow cytometric analysis.

Applications Tested: This 24DMS1 antibody has been tested by flow cytometric analysis of mouse splenocytes. This can be used at less than or equal to 0.25 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

Excitation: 488-561 nm; Emission: 578 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser.

Filtration: 0.2 µm post-manufacturing filtered.

Target Information

CD39 cell surface antigen is a 70-100kD molecule expressed on peripheral blood B cells, monocytes, T cell clones, and also weakly expressed on granulocytes. CD39 has intrinsic ecto-ATPase activity. Expression of CD39 is induced on T cells and increased on B cells as a late activation antigen.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Product References

Loss of C3a and C5a receptors promotes adipocyte browning and attenuates diet-induced obesity via activating inosine/A2aR pathway.

Cell reports

Kong LR,Chen XH,Sun Q,Zhang KY,Xu L,Ding L,Zhou YP,Zhang ZB,Lin JR,Gao PJ

12-0391-82 was used in Flow cytometry/Cell sorting to identify C3aR and C5aR as critical switches to control adipocyte browning and energy balance in male mice, via inosine/A2aR pathway.

Tue Feb 28 00:00:00 EST 2023

The transcription factor Bach2 negatively regulates murine natural killer cell maturation and function.

eLife

Li S,Bern MD,Miao B,Fan C,Xing X,Inoue T,Piersma SJ,Wang T,Colonna M,Kurosaki T,Yokoyama WM

12-0391-82 was used in Flow cytometry/Cell sorting to investigate the role of Bach2 in natural killer (NK) cell development, revealing that Bach2 expression peaked in immature CD27+CD11b+ cells, and its deficiency led to increased proportions of terminally differentiated NK cells with enhanced granzyme and cytokine production, as well as improved NK cell-mediated control of tumor metastasis.

Mon Oct 03 00:00:00 EDT 2022

Selective deletion of ENTPD1/CD39 in macrophages exacerbates biliary fibrosis in a mouse model of sclerosing cholangitis.

Purinergic signalling

Rothweiler S,Feldbrügge L,Jiang ZG,Csizmadia E,Longhi MS,Vaid K,Enjyoji K,Popov YV,Robson SC

Published figure using CD39 monoclonal antibody (Product # 12-0391-82) in Flow Cytometry

Sun Sep 01 00:00:00 EDT 2019

Toll-Like Receptor-Mediated Activation of CD39 Internalization in BMDCs Leads to Extracellular ATP Accumulation and Facilitates P2X7 Receptor Activation.

Frontiers in immunology

Zhao R,Qiao J,Zhang X,Zhao Y,Meng X,Sun D,Peng X

Published figure using CD39 monoclonal antibody (Product # 12-0391-82) in Flow Cytometry

Thu Oct 15 00:00:00 EDT 2020

Bacteroides fragilis polysaccharide A induces IL-10 secreting B and T cells that prevent viral encephalitis.

Nature communications

Ramakrishna C,Kujawski M,Chu H,Li L,Mazmanian SK,Cantin EM

12-0391 was used in Flow cytometry/Cell sorting to study polysaccharide A as an immunomodulatory symbiosis factor whcih coordinates anti-inflammatory responses during viral infections.

Tue May 14 00:00:00 EDT 2019

CD150^high Bone Marrow Tregs Maintain Hematopoietic Stem Cell Quiescence and Immune Privilege via Adenosine.

Cell stem cell

Hirata Y,Furuhashi K,Ishii H,Li HW,Pinho S,Ding L,Robson SC,Frenette PS,Fujisaki J

Published figure using CD39 monoclonal antibody (Product # 12-0391-82) in Flow Cytometry

Thu Mar 01 00:00:00 EST 2018

Response of regulatory T cells to classic heat stroke in mice.

Experimental and therapeutic medicine

Hu J,Kang HJ,Liu C,Hu P,Yang MM,Zhou FH

12-0391-82 was used in Flow cytometry/Cell sorting to investigate the splenic Treg response in a classic heat stroke mouse model.

Sat Dec 01 00:00:00 EST 2018

Dichotomous Expression of TNF Superfamily Ligands on Antigen-Presenting Cells Controls Post-priming Anti-viral CD4⁺ T Cell Immunity.

Immunity

Chang YH,Wang KC,Chu KL,Clouthier DL,Tran AT,Torres Perez MS,Zhou AC,Abdul-Sater AA,Watts TH

12-0391-82 was used in Flow Cytometry to investigate how different subtypes of T cell antigen-presenting cell contribute to determining viral set point and disease outcome.

Tue Nov 21 00:00:00 EST 2017

Differentiation of human and murine induced pluripotent stem cells to microglia-like cells.

Nature neuroscience

Pandya H,Shen MJ,Ichikawa DM,Sedlock AB,Choi Y,Johnson KR,Kim G,Brown MA,Elkahloun AG,Maric D,Sweeney CL,Gossa S,Malech HL,McGavern DB,Park JK

Published figure using CD39 monoclonal antibody (Product # 12-0391-82) in Flow Cytometry

Mon May 01 00:00:00 EDT 2017

IL-12 and IL-4 activate a CD39-dependent intrinsic peripheral tolerance mechanism in CD8(+) T cells.

European journal of immunology

Noble A,Mehta H,Lovell A,Papaioannou E,Fairbanks L

12-0391 was used in Flow cytometry/Cell sorting to study the upregulation of CD39 on CD8(+) T cells and identify a novel pathway of peripheral tolerance.

Wed Jun 01 00:00:00 EDT 2016

Developmental Progression and Interrelationship of Central and Effector Regulatory T Cell Subsets.

Journal of immunology (Baltimore, Md. : 1950)

Toomer KH,Yuan X,Yang J,Dee MJ,Yu A,Malek TR

12-0391 was used in Flow cytometry/Cell sorting to elucidate the developmental progression and interrelationship of central and effector regulatory T cell subsets.

Sun May 01 00:00:00 EDT 2016

Vitamin A Impairs the Reprogramming of Tregs into IL-17-Producing Cells during Intestinal Inflammation.

BioMed research international

Tejón G,Manríquez V,De Calisto J,Flores-Santibáñez F,Hidalgo Y,Crisóstomo N,Fernández D,Sauma D,Mora JR,Bono MR,Rosemblatt M

12-0391 was used in Flow cytometry/Cell sorting to study the contribution of different factors to the reprogramming of iTregs into IL-17-producing cells, showing that vitamin A impairs reprogramming during intestinal inflammation.

Fri Sep 02 00:00:00 EDT 2016

CD39 modulates hematopoietic stem cell recruitment and promotes liver regeneration in mice and humans after partial hepatectomy.

Annals of surgery

Schmelzle M,Duhme C,Junger W,Salhanick SD,Chen Y,Wu Y,Toxavidis V,Csizmadia E,Han L,Bian S,Fürst G,Nowak M,Karp SJ,Knoefel WT,Esch JSA,Robson SC

12-0391 was used in Flow cytometry/Cell sorting to explore the effect of CD39 family-derived ectonucleotidases on hematopoietic stem cell mobility and function following liver injury.

Mon Apr 01 00:00:00 EDT 2013

Butyrophilin-like 2 modulates B7 costimulation to induce Foxp3 expression and regulatory T cell development in mature T cells.

Journal of immunology (Baltimore, Md. : 1950)

Swanson RM,Gavin MA,Escobar SS,Rottman JB,Lipsky BP,Dube S,Li L,Bigler J,Wolfson M,Arnett HA,Viney JL

12-0391 was used in Flow cytometry/Cell sorting to investigate the mechanism by which butyrophilin-like 2 modulates B7 costimulation and regulatory T cell development.

Fri Mar 01 00:00:00 EST 2013

Radiation enhances regulatory T cell representation.

International journal of radiation oncology, biology, physics

Kachikwu EL,Iwamoto KS,Liao YP,DeMarco JJ,Agazaryan N,Economou JS,McBride WH,Schaue D

12-0391 was used in Flow cytometry/Cell sorting to suggest that Tregs are a radio-resistant subset of lymphocytes that may play a role in tumour evasion during radiotherapy.

Tue Nov 15 00:00:00 EST 2011

Self-regulation of inflammatory cell trafficking in mice by the leukocyte surface apyrase CD39.

The Journal of clinical investigation

Hyman MC,Petrovic-Djergovic D,Visovatti SH,Liao H,Yanamadala S,Bouïs D,Su EJ,Lawrence DA,Broekman MJ,Marcus AJ,Pinsky DJ

Fri May 01 00:00:00 EDT 2009

Bioinformatics

Protein Aliases: ATP diphosphohydrolase; ATP-DPH; CD39; DKFZp686D194; DKFZp686I093; Ecto-apyrase; Ecto-ATP diphosphohydrolase 1; Ecto-ATPDase 1; Ectonucleoside triphosphate diphosphohydrolase 1; FLJ40921; FLJ40959; Lymphoid cell activation antigen; NTPDase 1; NTPDase1; Nucleoside triphosphate diphosphohydrolase 1

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Gene Aliases: 2610206B08Rik; AA408691; Cd39; Entpd1; NTPDase-1

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UniProt ID: (Mouse) P55772

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Entrez Gene ID: (Mouse) 12495

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Function(s)

hydrolase nucleotide phosphatase phosphatase metabolite interconversion enzyme

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CD39 Monoclonal Antibody (24DMS1), PE, eBioscience™

CD39 Antibody (12-0391-82) in Flow

Product Details

12-0391-82

Flow Cytometry (Flow)

Species Reactivity

Published species

Host/Isotype

Recommended Isotype Control

Class

Type

Clone

Conjugate

Excitation/Emission Max

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

How to use the Panel Builder

Bioinformatics

Performance Guarantee

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